Nanoscale separation of molecular species based on their rotational mobility
- Autores
- Testa, Ilaria; Schönle, Andreas; V. Middendorff, Claas; Geisler, Claudia; Medda, Rebecca; Wurm, Christian A.; Stiel, Andre C.; Jakobs, Stefan; Bossi, Mariano Luis; Eggeling, Christian; Hell, Stefan W.; Egner, Alexander
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We combine far-field fluorescence nanoscopy through serializedrecording of switchable emitters with polarization-sensitive fluorescencedetection. In addition to imaging with nanoscale spatial resolution, thistechnique allows determination of the fluorescence anisotropy of eachdetected dipole emitter and thus an estimate of its rotational mobility. Subpopulationsof fluorescent markers can thus be separated based on theirinteraction with the sample. We applied this new functional nanoscopy toimaging of living mammalian cells.
Fil: Testa, Ilaria. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Schönle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: V. Middendorff, Claas. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Geisler, Claudia. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Stiel, Andre C.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania - Materia
-
Fluorescence
Microscopy
biological imaging - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/103202
Ver los metadatos del registro completo
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Nanoscale separation of molecular species based on their rotational mobilityTesta, IlariaSchönle, AndreasV. Middendorff, ClaasGeisler, ClaudiaMedda, RebeccaWurm, Christian A.Stiel, Andre C.Jakobs, StefanBossi, Mariano LuisEggeling, ChristianHell, Stefan W.Egner, AlexanderFluorescenceMicroscopybiological imaginghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We combine far-field fluorescence nanoscopy through serializedrecording of switchable emitters with polarization-sensitive fluorescencedetection. In addition to imaging with nanoscale spatial resolution, thistechnique allows determination of the fluorescence anisotropy of eachdetected dipole emitter and thus an estimate of its rotational mobility. Subpopulationsof fluorescent markers can thus be separated based on theirinteraction with the sample. We applied this new functional nanoscopy toimaging of living mammalian cells.Fil: Testa, Ilaria. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Schönle, Andreas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: V. Middendorff, Claas. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Geisler, Claudia. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Stiel, Andre C.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bossi, Mariano Luis. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; AlemaniaOptical Society of America2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103202Testa, Ilaria; Schönle, Andreas; V. Middendorff, Claas; Geisler, Claudia; Medda, Rebecca; et al.; Nanoscale separation of molecular species based on their rotational mobility; Optical Society of America; Optics Express; 16; 25; 12-2008; 21093-211041094-4087CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/oe/abstract.cfm?uri=oe-16-25-21093info:eu-repo/semantics/altIdentifier/doi/10.1364/OE.16.021093info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:03Zoai:ri.conicet.gov.ar:11336/103202instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:03.533CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Nanoscale separation of molecular species based on their rotational mobility |
| title |
Nanoscale separation of molecular species based on their rotational mobility |
| spellingShingle |
Nanoscale separation of molecular species based on their rotational mobility Testa, Ilaria Fluorescence Microscopy biological imaging |
| title_short |
Nanoscale separation of molecular species based on their rotational mobility |
| title_full |
Nanoscale separation of molecular species based on their rotational mobility |
| title_fullStr |
Nanoscale separation of molecular species based on their rotational mobility |
| title_full_unstemmed |
Nanoscale separation of molecular species based on their rotational mobility |
| title_sort |
Nanoscale separation of molecular species based on their rotational mobility |
| dc.creator.none.fl_str_mv |
Testa, Ilaria Schönle, Andreas V. Middendorff, Claas Geisler, Claudia Medda, Rebecca Wurm, Christian A. Stiel, Andre C. Jakobs, Stefan Bossi, Mariano Luis Eggeling, Christian Hell, Stefan W. Egner, Alexander |
| author |
Testa, Ilaria |
| author_facet |
Testa, Ilaria Schönle, Andreas V. Middendorff, Claas Geisler, Claudia Medda, Rebecca Wurm, Christian A. Stiel, Andre C. Jakobs, Stefan Bossi, Mariano Luis Eggeling, Christian Hell, Stefan W. Egner, Alexander |
| author_role |
author |
| author2 |
Schönle, Andreas V. Middendorff, Claas Geisler, Claudia Medda, Rebecca Wurm, Christian A. Stiel, Andre C. Jakobs, Stefan Bossi, Mariano Luis Eggeling, Christian Hell, Stefan W. Egner, Alexander |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Fluorescence Microscopy biological imaging |
| topic |
Fluorescence Microscopy biological imaging |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
We combine far-field fluorescence nanoscopy through serializedrecording of switchable emitters with polarization-sensitive fluorescencedetection. In addition to imaging with nanoscale spatial resolution, thistechnique allows determination of the fluorescence anisotropy of eachdetected dipole emitter and thus an estimate of its rotational mobility. Subpopulationsof fluorescent markers can thus be separated based on theirinteraction with the sample. We applied this new functional nanoscopy toimaging of living mammalian cells. Fil: Testa, Ilaria. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Schönle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania Fil: V. Middendorff, Claas. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Geisler, Claudia. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Stiel, Andre C.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Bossi, Mariano Luis. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania |
| description |
We combine far-field fluorescence nanoscopy through serializedrecording of switchable emitters with polarization-sensitive fluorescencedetection. In addition to imaging with nanoscale spatial resolution, thistechnique allows determination of the fluorescence anisotropy of eachdetected dipole emitter and thus an estimate of its rotational mobility. Subpopulationsof fluorescent markers can thus be separated based on theirinteraction with the sample. We applied this new functional nanoscopy toimaging of living mammalian cells. |
| publishDate |
2008 |
| dc.date.none.fl_str_mv |
2008-12 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/103202 Testa, Ilaria; Schönle, Andreas; V. Middendorff, Claas; Geisler, Claudia; Medda, Rebecca; et al.; Nanoscale separation of molecular species based on their rotational mobility; Optical Society of America; Optics Express; 16; 25; 12-2008; 21093-21104 1094-4087 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/103202 |
| identifier_str_mv |
Testa, Ilaria; Schönle, Andreas; V. Middendorff, Claas; Geisler, Claudia; Medda, Rebecca; et al.; Nanoscale separation of molecular species based on their rotational mobility; Optical Society of America; Optics Express; 16; 25; 12-2008; 21093-21104 1094-4087 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.osapublishing.org/oe/abstract.cfm?uri=oe-16-25-21093 info:eu-repo/semantics/altIdentifier/doi/10.1364/OE.16.021093 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Optical Society of America |
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Optical Society of America |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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