Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings

Autores
Tonón, Claudia Virginia; Terrile, Maria Cecilia; Iglesias, María José; Lamattina, Lorenzo; Casalongue, Claudia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Etiolated Arabidopsis thaliana seedlings germinated in the presence of reducing buffers such as reduced gluthathione (GSH) and dithiothreitol (DTT) have altered morphology. GSH and DTT inhibited hypocotyl elongation in a dose-dependent manner. The GSH-mediated effect was prevented by the simultaneous addition of extracellular ATP (eATP). NADPH oxidase (NOX) activity and endogenous nitric oxide (NO) generation were required to mediate eATP action on the hypocotyl elongation. A correlation was observed between hypocotyl length, eATP concentration and NO production. The action of eATP and NO on superoxide accumulation and peroxidase activity was investigated. The superoxide distribution was regulated by eATP and NO during hypocotyl elongation. Our data suggest that a finely tuned balance of redox status and optimal levels of ATP and NO are essential to regulate the hypocotyl elongation in the dark.
Fil: Tonón, Claudia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Terrile, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Iglesias, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
ARABIDOPSIS THALIANA
EXTRACELLULAR ATP
GLUTATHIONE
HYPOCOTYL ELONGATION
NITRIC OXIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133991
Acceder
id CONICETDig_a70922de854090f0354463918f93df5d
oai_identifier_str oai:ri.conicet.gov.ar:11336/133991
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlingsTonón, Claudia VirginiaTerrile, Maria CeciliaIglesias, María JoséLamattina, LorenzoCasalongue, ClaudiaARABIDOPSIS THALIANAEXTRACELLULAR ATPGLUTATHIONEHYPOCOTYL ELONGATIONNITRIC OXIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Etiolated Arabidopsis thaliana seedlings germinated in the presence of reducing buffers such as reduced gluthathione (GSH) and dithiothreitol (DTT) have altered morphology. GSH and DTT inhibited hypocotyl elongation in a dose-dependent manner. The GSH-mediated effect was prevented by the simultaneous addition of extracellular ATP (eATP). NADPH oxidase (NOX) activity and endogenous nitric oxide (NO) generation were required to mediate eATP action on the hypocotyl elongation. A correlation was observed between hypocotyl length, eATP concentration and NO production. The action of eATP and NO on superoxide accumulation and peroxidase activity was investigated. The superoxide distribution was regulated by eATP and NO during hypocotyl elongation. Our data suggest that a finely tuned balance of redox status and optimal levels of ATP and NO are essential to regulate the hypocotyl elongation in the dark.Fil: Tonón, Claudia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Terrile, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Iglesias, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaElsevier Gmbh2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133991Tonón, Claudia Virginia; Terrile, Maria Cecilia; Iglesias, María José; Lamattina, Lorenzo; Casalongue, Claudia; Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings; Elsevier Gmbh; Journal of Plant Physiology; 167; 7; 12-2009; 540-5460176-1617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2009.11.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:29:26Zoai:ri.conicet.gov.ar:11336/133991instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:29:26.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
title Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
spellingShingle Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
Tonón, Claudia Virginia
ARABIDOPSIS THALIANA
EXTRACELLULAR ATP
GLUTATHIONE
HYPOCOTYL ELONGATION
NITRIC OXIDE
title_short Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
title_full Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
title_fullStr Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
title_full_unstemmed Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
title_sort Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
dc.creator.none.fl_str_mv Tonón, Claudia Virginia
Terrile, Maria Cecilia
Iglesias, María José
Lamattina, Lorenzo
Casalongue, Claudia
author Tonón, Claudia Virginia
author_facet Tonón, Claudia Virginia
Terrile, Maria Cecilia
Iglesias, María José
Lamattina, Lorenzo
Casalongue, Claudia
author_role author
author2 Terrile, Maria Cecilia
Iglesias, María José
Lamattina, Lorenzo
Casalongue, Claudia
author2_role author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS THALIANA
EXTRACELLULAR ATP
GLUTATHIONE
HYPOCOTYL ELONGATION
NITRIC OXIDE
topic ARABIDOPSIS THALIANA
EXTRACELLULAR ATP
GLUTATHIONE
HYPOCOTYL ELONGATION
NITRIC OXIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Etiolated Arabidopsis thaliana seedlings germinated in the presence of reducing buffers such as reduced gluthathione (GSH) and dithiothreitol (DTT) have altered morphology. GSH and DTT inhibited hypocotyl elongation in a dose-dependent manner. The GSH-mediated effect was prevented by the simultaneous addition of extracellular ATP (eATP). NADPH oxidase (NOX) activity and endogenous nitric oxide (NO) generation were required to mediate eATP action on the hypocotyl elongation. A correlation was observed between hypocotyl length, eATP concentration and NO production. The action of eATP and NO on superoxide accumulation and peroxidase activity was investigated. The superoxide distribution was regulated by eATP and NO during hypocotyl elongation. Our data suggest that a finely tuned balance of redox status and optimal levels of ATP and NO are essential to regulate the hypocotyl elongation in the dark.
Fil: Tonón, Claudia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Terrile, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Iglesias, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Etiolated Arabidopsis thaliana seedlings germinated in the presence of reducing buffers such as reduced gluthathione (GSH) and dithiothreitol (DTT) have altered morphology. GSH and DTT inhibited hypocotyl elongation in a dose-dependent manner. The GSH-mediated effect was prevented by the simultaneous addition of extracellular ATP (eATP). NADPH oxidase (NOX) activity and endogenous nitric oxide (NO) generation were required to mediate eATP action on the hypocotyl elongation. A correlation was observed between hypocotyl length, eATP concentration and NO production. The action of eATP and NO on superoxide accumulation and peroxidase activity was investigated. The superoxide distribution was regulated by eATP and NO during hypocotyl elongation. Our data suggest that a finely tuned balance of redox status and optimal levels of ATP and NO are essential to regulate the hypocotyl elongation in the dark.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133991
Tonón, Claudia Virginia; Terrile, Maria Cecilia; Iglesias, María José; Lamattina, Lorenzo; Casalongue, Claudia; Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings; Elsevier Gmbh; Journal of Plant Physiology; 167; 7; 12-2009; 540-546
0176-1617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133991
identifier_str_mv Tonón, Claudia Virginia; Terrile, Maria Cecilia; Iglesias, María José; Lamattina, Lorenzo; Casalongue, Claudia; Extracellular ATP, nitric oxide and superoxide act coordinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings; Elsevier Gmbh; Journal of Plant Physiology; 167; 7; 12-2009; 540-546
0176-1617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2009.11.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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