Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia

Autores
Tolomeo, Anna Maria; Fabozzo, Assunta; Malvicini, Ricardo; De Lazzari, Giada; Bisaccia, Paola; Gaburro, Gianluca; Arcidiacono, Diletta; Notarangelo, Denni; Caicci, Federico; Zanella, Fabio; Marchesan, Massimo; Yannarelli, Gustavo Gabriel; Santovito, Gianfranco; Muraca, Maurizio; Gerosa, Gino
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Insufficient supply of cardiac grafts represents a severe obstacle in heart transplantation. Donation after circulatory death (DCD), in addition to conventional donation after brain death, is one promising option to overcome the organ shortage. However, DCD organs undergo an inevitably longer period of unprotected warm ischemia between circulatory arrest and graft procurement. In this scenario, we aim to improve heart preservation after a warm ischemic period of 20 min by testing different settings of myocardial protective strategies. Pig hearts were collected from a slaughterhouse and assigned to one of the five experimental groups: baseline (BL), cold cardioplegia (CC), cold cardioplegia + adenosine (CC-ADN), normothermic cardioplegia (NtC + CC) or normothermic cardioplegia + cold cardioplegia + adenosine (NtC-ADN + CC). After treatment, tissue biopsies were taken to assess mitochondrial morphology, antioxidant enzyme activity, lipid peroxidation and cytokine and chemokine expressions. NtC + CC treatment significantly prevented mitochondria swelling and mitochondrial cristae loss. Moreover, the antioxidant enzyme activity was lower in this group, as was lipid peroxidation, and the pro-inflammatory chemokine GM-CSF was diminished. Finally, we demonstrated that normothermic cardioplegia preserved mitochondria morphology, thus preventing oxidative stress and the subsequent inflammatory response. Therefore, normothermic cardioplegia is a better approach to preserve the heart after a warm ischemia period, with respect to cold cardioplegia, before transplantation.
Fil: Tolomeo, Anna Maria. Università di Padova; Italia
Fil: Fabozzo, Assunta. Università di Padova; Italia
Fil: Malvicini, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: De Lazzari, Giada. Università di Padova; Italia
Fil: Bisaccia, Paola. Università di Padova; Italia
Fil: Gaburro, Gianluca. Università di Padova; Italia
Fil: Arcidiacono, Diletta. Veneto Institute of Oncology; Italia
Fil: Notarangelo, Denni. Università di Padova; Italia
Fil: Caicci, Federico. Università di Padova; Italia
Fil: Zanella, Fabio. Università di Padova; Italia
Fil: Marchesan, Massimo. No especifíca;
Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Santovito, Gianfranco. Università di Padova; Italia
Fil: Muraca, Maurizio. Università di Padova; Italia
Fil: Gerosa, Gino. Università di Padova; Italia
Materia
DONATION AFTER CIRCULATORY DEATH
ENRICHED CARDIOPLEGIA
ORGAN RECONDITIONING
OXIDATIVE STRESS
TRANSPLANTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214460
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm IschemiaTolomeo, Anna MariaFabozzo, AssuntaMalvicini, RicardoDe Lazzari, GiadaBisaccia, PaolaGaburro, GianlucaArcidiacono, DilettaNotarangelo, DenniCaicci, FedericoZanella, FabioMarchesan, MassimoYannarelli, Gustavo GabrielSantovito, GianfrancoMuraca, MaurizioGerosa, GinoDONATION AFTER CIRCULATORY DEATHENRICHED CARDIOPLEGIAORGAN RECONDITIONINGOXIDATIVE STRESSTRANSPLANTATIONhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Insufficient supply of cardiac grafts represents a severe obstacle in heart transplantation. Donation after circulatory death (DCD), in addition to conventional donation after brain death, is one promising option to overcome the organ shortage. However, DCD organs undergo an inevitably longer period of unprotected warm ischemia between circulatory arrest and graft procurement. In this scenario, we aim to improve heart preservation after a warm ischemic period of 20 min by testing different settings of myocardial protective strategies. Pig hearts were collected from a slaughterhouse and assigned to one of the five experimental groups: baseline (BL), cold cardioplegia (CC), cold cardioplegia + adenosine (CC-ADN), normothermic cardioplegia (NtC + CC) or normothermic cardioplegia + cold cardioplegia + adenosine (NtC-ADN + CC). After treatment, tissue biopsies were taken to assess mitochondrial morphology, antioxidant enzyme activity, lipid peroxidation and cytokine and chemokine expressions. NtC + CC treatment significantly prevented mitochondria swelling and mitochondrial cristae loss. Moreover, the antioxidant enzyme activity was lower in this group, as was lipid peroxidation, and the pro-inflammatory chemokine GM-CSF was diminished. Finally, we demonstrated that normothermic cardioplegia preserved mitochondria morphology, thus preventing oxidative stress and the subsequent inflammatory response. Therefore, normothermic cardioplegia is a better approach to preserve the heart after a warm ischemia period, with respect to cold cardioplegia, before transplantation.Fil: Tolomeo, Anna Maria. Università di Padova; ItaliaFil: Fabozzo, Assunta. Università di Padova; ItaliaFil: Malvicini, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: De Lazzari, Giada. Università di Padova; ItaliaFil: Bisaccia, Paola. Università di Padova; ItaliaFil: Gaburro, Gianluca. Università di Padova; ItaliaFil: Arcidiacono, Diletta. Veneto Institute of Oncology; ItaliaFil: Notarangelo, Denni. Università di Padova; ItaliaFil: Caicci, Federico. Università di Padova; ItaliaFil: Zanella, Fabio. Università di Padova; ItaliaFil: Marchesan, Massimo. No especifíca;Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Santovito, Gianfranco. Università di Padova; ItaliaFil: Muraca, Maurizio. Università di Padova; ItaliaFil: Gerosa, Gino. Università di Padova; ItaliaMDPI2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214460Tolomeo, Anna Maria; Fabozzo, Assunta; Malvicini, Ricardo; De Lazzari, Giada; Bisaccia, Paola; et al.; Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia; MDPI; Antioxidants; 11; 3; 2-2022; 1-172076-3921CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/11/3/476info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox11030476info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:28Zoai:ri.conicet.gov.ar:11336/214460instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:29.059CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
title Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
spellingShingle Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
Tolomeo, Anna Maria
DONATION AFTER CIRCULATORY DEATH
ENRICHED CARDIOPLEGIA
ORGAN RECONDITIONING
OXIDATIVE STRESS
TRANSPLANTATION
title_short Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
title_full Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
title_fullStr Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
title_full_unstemmed Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
title_sort Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia
dc.creator.none.fl_str_mv Tolomeo, Anna Maria
Fabozzo, Assunta
Malvicini, Ricardo
De Lazzari, Giada
Bisaccia, Paola
Gaburro, Gianluca
Arcidiacono, Diletta
Notarangelo, Denni
Caicci, Federico
Zanella, Fabio
Marchesan, Massimo
Yannarelli, Gustavo Gabriel
Santovito, Gianfranco
Muraca, Maurizio
Gerosa, Gino
author Tolomeo, Anna Maria
author_facet Tolomeo, Anna Maria
Fabozzo, Assunta
Malvicini, Ricardo
De Lazzari, Giada
Bisaccia, Paola
Gaburro, Gianluca
Arcidiacono, Diletta
Notarangelo, Denni
Caicci, Federico
Zanella, Fabio
Marchesan, Massimo
Yannarelli, Gustavo Gabriel
Santovito, Gianfranco
Muraca, Maurizio
Gerosa, Gino
author_role author
author2 Fabozzo, Assunta
Malvicini, Ricardo
De Lazzari, Giada
Bisaccia, Paola
Gaburro, Gianluca
Arcidiacono, Diletta
Notarangelo, Denni
Caicci, Federico
Zanella, Fabio
Marchesan, Massimo
Yannarelli, Gustavo Gabriel
Santovito, Gianfranco
Muraca, Maurizio
Gerosa, Gino
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DONATION AFTER CIRCULATORY DEATH
ENRICHED CARDIOPLEGIA
ORGAN RECONDITIONING
OXIDATIVE STRESS
TRANSPLANTATION
topic DONATION AFTER CIRCULATORY DEATH
ENRICHED CARDIOPLEGIA
ORGAN RECONDITIONING
OXIDATIVE STRESS
TRANSPLANTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Insufficient supply of cardiac grafts represents a severe obstacle in heart transplantation. Donation after circulatory death (DCD), in addition to conventional donation after brain death, is one promising option to overcome the organ shortage. However, DCD organs undergo an inevitably longer period of unprotected warm ischemia between circulatory arrest and graft procurement. In this scenario, we aim to improve heart preservation after a warm ischemic period of 20 min by testing different settings of myocardial protective strategies. Pig hearts were collected from a slaughterhouse and assigned to one of the five experimental groups: baseline (BL), cold cardioplegia (CC), cold cardioplegia + adenosine (CC-ADN), normothermic cardioplegia (NtC + CC) or normothermic cardioplegia + cold cardioplegia + adenosine (NtC-ADN + CC). After treatment, tissue biopsies were taken to assess mitochondrial morphology, antioxidant enzyme activity, lipid peroxidation and cytokine and chemokine expressions. NtC + CC treatment significantly prevented mitochondria swelling and mitochondrial cristae loss. Moreover, the antioxidant enzyme activity was lower in this group, as was lipid peroxidation, and the pro-inflammatory chemokine GM-CSF was diminished. Finally, we demonstrated that normothermic cardioplegia preserved mitochondria morphology, thus preventing oxidative stress and the subsequent inflammatory response. Therefore, normothermic cardioplegia is a better approach to preserve the heart after a warm ischemia period, with respect to cold cardioplegia, before transplantation.
Fil: Tolomeo, Anna Maria. Università di Padova; Italia
Fil: Fabozzo, Assunta. Università di Padova; Italia
Fil: Malvicini, Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: De Lazzari, Giada. Università di Padova; Italia
Fil: Bisaccia, Paola. Università di Padova; Italia
Fil: Gaburro, Gianluca. Università di Padova; Italia
Fil: Arcidiacono, Diletta. Veneto Institute of Oncology; Italia
Fil: Notarangelo, Denni. Università di Padova; Italia
Fil: Caicci, Federico. Università di Padova; Italia
Fil: Zanella, Fabio. Università di Padova; Italia
Fil: Marchesan, Massimo. No especifíca;
Fil: Yannarelli, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Santovito, Gianfranco. Università di Padova; Italia
Fil: Muraca, Maurizio. Università di Padova; Italia
Fil: Gerosa, Gino. Università di Padova; Italia
description Insufficient supply of cardiac grafts represents a severe obstacle in heart transplantation. Donation after circulatory death (DCD), in addition to conventional donation after brain death, is one promising option to overcome the organ shortage. However, DCD organs undergo an inevitably longer period of unprotected warm ischemia between circulatory arrest and graft procurement. In this scenario, we aim to improve heart preservation after a warm ischemic period of 20 min by testing different settings of myocardial protective strategies. Pig hearts were collected from a slaughterhouse and assigned to one of the five experimental groups: baseline (BL), cold cardioplegia (CC), cold cardioplegia + adenosine (CC-ADN), normothermic cardioplegia (NtC + CC) or normothermic cardioplegia + cold cardioplegia + adenosine (NtC-ADN + CC). After treatment, tissue biopsies were taken to assess mitochondrial morphology, antioxidant enzyme activity, lipid peroxidation and cytokine and chemokine expressions. NtC + CC treatment significantly prevented mitochondria swelling and mitochondrial cristae loss. Moreover, the antioxidant enzyme activity was lower in this group, as was lipid peroxidation, and the pro-inflammatory chemokine GM-CSF was diminished. Finally, we demonstrated that normothermic cardioplegia preserved mitochondria morphology, thus preventing oxidative stress and the subsequent inflammatory response. Therefore, normothermic cardioplegia is a better approach to preserve the heart after a warm ischemia period, with respect to cold cardioplegia, before transplantation.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214460
Tolomeo, Anna Maria; Fabozzo, Assunta; Malvicini, Ricardo; De Lazzari, Giada; Bisaccia, Paola; et al.; Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia; MDPI; Antioxidants; 11; 3; 2-2022; 1-17
2076-3921
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214460
identifier_str_mv Tolomeo, Anna Maria; Fabozzo, Assunta; Malvicini, Ricardo; De Lazzari, Giada; Bisaccia, Paola; et al.; Temperature-Related Effects of Myocardial Protection Strategies in Swine Hearts after Prolonged Warm Ischemia; MDPI; Antioxidants; 11; 3; 2-2022; 1-17
2076-3921
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-3921/11/3/476
info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox11030476
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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