Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos

Autores
Muller, Mariel; Hess, Leonardo; Tardivo, Agustina; Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; Poletta, Gisela Laura; Simoniello, Maria Fernanda; Yodice, Agustina; Lavarello, Simona; Chialvo, Dante Renato; Scremin, Oscar Umberto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlorpyrifos (CPF) is an organophosphorus cholinesterase inhibitor widely used as an insecticide. Neuro and genotoxicity of this agent were evaluated following daily subcutaneous injections at 0.1, 1 and 10 mg/kg or its vehicle to laboratory rats during one week, at the end of which somatosensory evoked potentials (SEP) and power spectrum of the electroencephalogram (EEGp) were recorded under urethane anesthesia. In another group of conscious animals, auditory startle reflex (ASR) was evaluated followed, after euthanasia, with measurements of plasma B-esterases, and genotoxicity with the alkaline comet assay (ACA) at the same CPF doses. The results indicated a CPF dose related inhibition of B-esterases. Enhanced inhibition of the ASR by a subthreshold pre-pulse was observed at all doses and ACA showed a significant higher DNA damage than vehicle controls in animals exposed to 10 mg/kg CPF. A trend to higher frequencies of EEGp and an increase in amplitude of the first negative wave of the SEP were found at all doses. The first positive wave of the SEP decreased at the CPF dose of 10 mg/kg. In summary, a shift to higher EEG frequencies and alterations of somatosensory and auditory input to the central nervous system were sensitive manifestations of CPF toxicity, associated with depression of B-esterases. The changes in electrical activity of the cerebral cortex and DNA damage observed at doses that do not elicit overt toxicity may be useful in the detection of CPF exposure before clinical signs appear.
Fil: Muller, Mariel. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Hess, Leonardo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Tardivo, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Poletta, Gisela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
Fil: Yodice, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Lavarello, Simona. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Chialvo, Dante Renato. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scremin, Oscar Umberto. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. University Of California At Los Angeles. School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Acetylcholinesterase Inhibitors
Comet Assay
Auditory Startle
Eeg
Evoked Potentials
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30417
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30417
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifosMuller, MarielHess, LeonardoTardivo, AgustinaLajmanovich, Rafael CarlosAttademo, Andres MaximilianoPoletta, Gisela LauraSimoniello, Maria FernandaYodice, AgustinaLavarello, SimonaChialvo, Dante RenatoScremin, Oscar UmbertoAcetylcholinesterase InhibitorsComet AssayAuditory StartleEegEvoked Potentialshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chlorpyrifos (CPF) is an organophosphorus cholinesterase inhibitor widely used as an insecticide. Neuro and genotoxicity of this agent were evaluated following daily subcutaneous injections at 0.1, 1 and 10 mg/kg or its vehicle to laboratory rats during one week, at the end of which somatosensory evoked potentials (SEP) and power spectrum of the electroencephalogram (EEGp) were recorded under urethane anesthesia. In another group of conscious animals, auditory startle reflex (ASR) was evaluated followed, after euthanasia, with measurements of plasma B-esterases, and genotoxicity with the alkaline comet assay (ACA) at the same CPF doses. The results indicated a CPF dose related inhibition of B-esterases. Enhanced inhibition of the ASR by a subthreshold pre-pulse was observed at all doses and ACA showed a significant higher DNA damage than vehicle controls in animals exposed to 10 mg/kg CPF. A trend to higher frequencies of EEGp and an increase in amplitude of the first negative wave of the SEP were found at all doses. The first positive wave of the SEP decreased at the CPF dose of 10 mg/kg. In summary, a shift to higher EEG frequencies and alterations of somatosensory and auditory input to the central nervous system were sensitive manifestations of CPF toxicity, associated with depression of B-esterases. The changes in electrical activity of the cerebral cortex and DNA damage observed at doses that do not elicit overt toxicity may be useful in the detection of CPF exposure before clinical signs appear.Fil: Muller, Mariel. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Hess, Leonardo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Tardivo, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Poletta, Gisela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; ArgentinaFil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; ArgentinaFil: Yodice, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Lavarello, Simona. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; ArgentinaFil: Chialvo, Dante Renato. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scremin, Oscar Umberto. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. University Of California At Los Angeles. School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30417Muller, Mariel; Hess, Leonardo; Tardivo, Agustina; Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; et al.; Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos; Elsevier; Neurotoxicology And Teratology.; 45; 9-2014; 22-300892-0362CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuro.2014.08.012info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0161813X1400151Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:30:43Zoai:ri.conicet.gov.ar:11336/30417instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:30:43.673CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
title Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
spellingShingle Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
Muller, Mariel
Acetylcholinesterase Inhibitors
Comet Assay
Auditory Startle
Eeg
Evoked Potentials
title_short Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
title_full Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
title_fullStr Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
title_full_unstemmed Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
title_sort Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos
dc.creator.none.fl_str_mv Muller, Mariel
Hess, Leonardo
Tardivo, Agustina
Lajmanovich, Rafael Carlos
Attademo, Andres Maximiliano
Poletta, Gisela Laura
Simoniello, Maria Fernanda
Yodice, Agustina
Lavarello, Simona
Chialvo, Dante Renato
Scremin, Oscar Umberto
author Muller, Mariel
author_facet Muller, Mariel
Hess, Leonardo
Tardivo, Agustina
Lajmanovich, Rafael Carlos
Attademo, Andres Maximiliano
Poletta, Gisela Laura
Simoniello, Maria Fernanda
Yodice, Agustina
Lavarello, Simona
Chialvo, Dante Renato
Scremin, Oscar Umberto
author_role author
author2 Hess, Leonardo
Tardivo, Agustina
Lajmanovich, Rafael Carlos
Attademo, Andres Maximiliano
Poletta, Gisela Laura
Simoniello, Maria Fernanda
Yodice, Agustina
Lavarello, Simona
Chialvo, Dante Renato
Scremin, Oscar Umberto
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Acetylcholinesterase Inhibitors
Comet Assay
Auditory Startle
Eeg
Evoked Potentials
topic Acetylcholinesterase Inhibitors
Comet Assay
Auditory Startle
Eeg
Evoked Potentials
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chlorpyrifos (CPF) is an organophosphorus cholinesterase inhibitor widely used as an insecticide. Neuro and genotoxicity of this agent were evaluated following daily subcutaneous injections at 0.1, 1 and 10 mg/kg or its vehicle to laboratory rats during one week, at the end of which somatosensory evoked potentials (SEP) and power spectrum of the electroencephalogram (EEGp) were recorded under urethane anesthesia. In another group of conscious animals, auditory startle reflex (ASR) was evaluated followed, after euthanasia, with measurements of plasma B-esterases, and genotoxicity with the alkaline comet assay (ACA) at the same CPF doses. The results indicated a CPF dose related inhibition of B-esterases. Enhanced inhibition of the ASR by a subthreshold pre-pulse was observed at all doses and ACA showed a significant higher DNA damage than vehicle controls in animals exposed to 10 mg/kg CPF. A trend to higher frequencies of EEGp and an increase in amplitude of the first negative wave of the SEP were found at all doses. The first positive wave of the SEP decreased at the CPF dose of 10 mg/kg. In summary, a shift to higher EEG frequencies and alterations of somatosensory and auditory input to the central nervous system were sensitive manifestations of CPF toxicity, associated with depression of B-esterases. The changes in electrical activity of the cerebral cortex and DNA damage observed at doses that do not elicit overt toxicity may be useful in the detection of CPF exposure before clinical signs appear.
Fil: Muller, Mariel. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Hess, Leonardo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Tardivo, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Poletta, Gisela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina
Fil: Yodice, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Lavarello, Simona. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina
Fil: Chialvo, Dante Renato. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scremin, Oscar Umberto. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina. University Of California At Los Angeles. School Of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Chlorpyrifos (CPF) is an organophosphorus cholinesterase inhibitor widely used as an insecticide. Neuro and genotoxicity of this agent were evaluated following daily subcutaneous injections at 0.1, 1 and 10 mg/kg or its vehicle to laboratory rats during one week, at the end of which somatosensory evoked potentials (SEP) and power spectrum of the electroencephalogram (EEGp) were recorded under urethane anesthesia. In another group of conscious animals, auditory startle reflex (ASR) was evaluated followed, after euthanasia, with measurements of plasma B-esterases, and genotoxicity with the alkaline comet assay (ACA) at the same CPF doses. The results indicated a CPF dose related inhibition of B-esterases. Enhanced inhibition of the ASR by a subthreshold pre-pulse was observed at all doses and ACA showed a significant higher DNA damage than vehicle controls in animals exposed to 10 mg/kg CPF. A trend to higher frequencies of EEGp and an increase in amplitude of the first negative wave of the SEP were found at all doses. The first positive wave of the SEP decreased at the CPF dose of 10 mg/kg. In summary, a shift to higher EEG frequencies and alterations of somatosensory and auditory input to the central nervous system were sensitive manifestations of CPF toxicity, associated with depression of B-esterases. The changes in electrical activity of the cerebral cortex and DNA damage observed at doses that do not elicit overt toxicity may be useful in the detection of CPF exposure before clinical signs appear.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30417
Muller, Mariel; Hess, Leonardo; Tardivo, Agustina; Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; et al.; Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos; Elsevier; Neurotoxicology And Teratology.; 45; 9-2014; 22-30
0892-0362
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30417
identifier_str_mv Muller, Mariel; Hess, Leonardo; Tardivo, Agustina; Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; et al.; Neurologic dysfunction and genotoxicity induced by low levels of chlorpyrifos; Elsevier; Neurotoxicology And Teratology.; 45; 9-2014; 22-30
0892-0362
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuro.2014.08.012
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0161813X1400151X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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