Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells

Autores
Marcon, Bruna H.; Holetz, Fabíola B.; Eastman, Guillermo; Origa Alves, Ana Carolina; Amorós, Mariana Andrea; de Aguiar, Alessandra Melo; Rebelatto, Carmen K.; Brofman, Paulo R.S.; Sotelo-Silveira, Jose; Dallagiovanna, Bruno
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction of whole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteins were downregulated at the translational level. This negative regulation of the essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1, a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational regulation occurred during the early stage of the adipogenic differentiation of hASCs.
Fil: Marcon, Bruna H.. Fundación Oswaldo Cruz; Brasil
Fil: Holetz, Fabíola B.. Fundación Oswaldo Cruz; Brasil
Fil: Eastman, Guillermo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Origa Alves, Ana Carolina. Fundación Oswaldo Cruz; Brasil
Fil: Amorós, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: de Aguiar, Alessandra Melo. Fundación Oswaldo Cruz; Brasil
Fil: Rebelatto, Carmen K.. Pontificia Universidade Catolica Do Parana; Brasil
Fil: Brofman, Paulo R.S.. Pontificia Universidade Catolica Do Parana; Brasil
Fil: Sotelo-Silveira, Jose. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay. Universidad de la República; Uruguay
Fil: Dallagiovanna, Bruno. Fundación Oswaldo Cruz; Brasil
Materia
ADIPOGENESIS
HASCS
RIBOSOME PROFILING
TRANSLATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64505
Acceder
id CONICETDig_a6173e9b4908c243db8d69929428f06c
oai_identifier_str oai:ri.conicet.gov.ar:11336/64505
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cellsMarcon, Bruna H.Holetz, Fabíola B.Eastman, GuillermoOriga Alves, Ana CarolinaAmorós, Mariana Andreade Aguiar, Alessandra MeloRebelatto, Carmen K.Brofman, Paulo R.S.Sotelo-Silveira, JoseDallagiovanna, BrunoADIPOGENESISHASCSRIBOSOME PROFILINGTRANSLATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction of whole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteins were downregulated at the translational level. This negative regulation of the essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1, a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational regulation occurred during the early stage of the adipogenic differentiation of hASCs.Fil: Marcon, Bruna H.. Fundación Oswaldo Cruz; BrasilFil: Holetz, Fabíola B.. Fundación Oswaldo Cruz; BrasilFil: Eastman, Guillermo. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Origa Alves, Ana Carolina. Fundación Oswaldo Cruz; BrasilFil: Amorós, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: de Aguiar, Alessandra Melo. Fundación Oswaldo Cruz; BrasilFil: Rebelatto, Carmen K.. Pontificia Universidade Catolica Do Parana; BrasilFil: Brofman, Paulo R.S.. Pontificia Universidade Catolica Do Parana; BrasilFil: Sotelo-Silveira, Jose. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay. Universidad de la República; UruguayFil: Dallagiovanna, Bruno. Fundación Oswaldo Cruz; BrasilElsevier Science2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64505Marcon, Bruna H.; Holetz, Fabíola B.; Eastman, Guillermo; Origa Alves, Ana Carolina; Amorós, Mariana Andrea; et al.; Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells; Elsevier Science; Stem Cell Research; 25; 12-2017; 191-2011873-5061CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scr.2017.10.027info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1873506117302301info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:04Zoai:ri.conicet.gov.ar:11336/64505instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:04.865CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
title Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
spellingShingle Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
Marcon, Bruna H.
ADIPOGENESIS
HASCS
RIBOSOME PROFILING
TRANSLATION
title_short Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
title_full Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
title_fullStr Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
title_full_unstemmed Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
title_sort Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
dc.creator.none.fl_str_mv Marcon, Bruna H.
Holetz, Fabíola B.
Eastman, Guillermo
Origa Alves, Ana Carolina
Amorós, Mariana Andrea
de Aguiar, Alessandra Melo
Rebelatto, Carmen K.
Brofman, Paulo R.S.
Sotelo-Silveira, Jose
Dallagiovanna, Bruno
author Marcon, Bruna H.
author_facet Marcon, Bruna H.
Holetz, Fabíola B.
Eastman, Guillermo
Origa Alves, Ana Carolina
Amorós, Mariana Andrea
de Aguiar, Alessandra Melo
Rebelatto, Carmen K.
Brofman, Paulo R.S.
Sotelo-Silveira, Jose
Dallagiovanna, Bruno
author_role author
author2 Holetz, Fabíola B.
Eastman, Guillermo
Origa Alves, Ana Carolina
Amorós, Mariana Andrea
de Aguiar, Alessandra Melo
Rebelatto, Carmen K.
Brofman, Paulo R.S.
Sotelo-Silveira, Jose
Dallagiovanna, Bruno
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADIPOGENESIS
HASCS
RIBOSOME PROFILING
TRANSLATION
topic ADIPOGENESIS
HASCS
RIBOSOME PROFILING
TRANSLATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction of whole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteins were downregulated at the translational level. This negative regulation of the essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1, a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational regulation occurred during the early stage of the adipogenic differentiation of hASCs.
Fil: Marcon, Bruna H.. Fundación Oswaldo Cruz; Brasil
Fil: Holetz, Fabíola B.. Fundación Oswaldo Cruz; Brasil
Fil: Eastman, Guillermo. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Origa Alves, Ana Carolina. Fundación Oswaldo Cruz; Brasil
Fil: Amorós, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: de Aguiar, Alessandra Melo. Fundación Oswaldo Cruz; Brasil
Fil: Rebelatto, Carmen K.. Pontificia Universidade Catolica Do Parana; Brasil
Fil: Brofman, Paulo R.S.. Pontificia Universidade Catolica Do Parana; Brasil
Fil: Sotelo-Silveira, Jose. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay. Universidad de la República; Uruguay
Fil: Dallagiovanna, Bruno. Fundación Oswaldo Cruz; Brasil
description Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction of whole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteins were downregulated at the translational level. This negative regulation of the essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1, a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational regulation occurred during the early stage of the adipogenic differentiation of hASCs.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64505
Marcon, Bruna H.; Holetz, Fabíola B.; Eastman, Guillermo; Origa Alves, Ana Carolina; Amorós, Mariana Andrea; et al.; Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells; Elsevier Science; Stem Cell Research; 25; 12-2017; 191-201
1873-5061
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64505
identifier_str_mv Marcon, Bruna H.; Holetz, Fabíola B.; Eastman, Guillermo; Origa Alves, Ana Carolina; Amorós, Mariana Andrea; et al.; Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells; Elsevier Science; Stem Cell Research; 25; 12-2017; 191-201
1873-5061
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scr.2017.10.027
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1873506117302301
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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