Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed

Autores
Rybak Wolf, Agnieszka; Stottmeister, Christin; Glažar, Petar; Jens, Marvin; Pino, Natalia; Giusti, Sebastian; Hanan, Mor; Behm, Mikaela; Bartok, Osnat; Ashwal Fluss, Reut; Herzog, Margareta; Schreyer, Luisa; Papavasileiou, Panagiotis; Ivanov, Andranik; Öhman, Marie; Refojo, Damian; Kadener, Sebastian; Rajewsky, Nikolaus
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite their abundance, their function and expression in the nervous system are unknown. Therefore, we sequenced RNA from different brain regions, primary neurons, isolated synapses, as well as during neuronal differentiation. Using these and other available data, we discovered and analyzed thousands of neuronal human and mouse circRNAs. circRNAs were extraordinarily enriched in the mammalian brain, well conserved in sequence, often expressed as circRNAs in both human and mouse, and sometimes even detected in Drosophila brains. circRNAs were overall upregulated during neuronal differentiation, highly enriched in synapses, and often differentially expressed compared to their mRNA isoforms. circRNA expression correlated negatively with expression of the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated circRNA expression. Together, we provide a circRNA brain expression atlas and evidence for important circRNA functions and values as biomarkers.
Fil: Rybak Wolf, Agnieszka. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Stottmeister, Christin. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Glažar, Petar. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Jens, Marvin. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Pino, Natalia. Max Planck Institute of Psychiatry; Alemania
Fil: Giusti, Sebastian. Max Planck Institute of Psychiatry; Alemania
Fil: Hanan, Mor. The Hebrew University of Jerusalem; Israel
Fil: Behm, Mikaela. Stockholms Universitet; Suecia
Fil: Bartok, Osnat. The Hebrew University of Jerusalem; Israel
Fil: Ashwal Fluss, Reut. The Hebrew University of Jerusalem; Israel
Fil: Herzog, Margareta. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Schreyer, Luisa. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Papavasileiou, Panagiotis. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Ivanov, Andranik. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Öhman, Marie. Stockholms Universitet; Suecia
Fil: Refojo, Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina. Max Planck Institute of Psychiatry; Alemania
Fil: Kadener, Sebastian. The Hebrew University of Jerusalem; Israel
Fil: Rajewsky, Nikolaus. Max-Delbrück Center for Molecular Medicine; Alemania
Materia
circular RNAs
non-coding RNA
brain
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85840
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85840
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically ExpressedRybak Wolf, AgnieszkaStottmeister, ChristinGlažar, PetarJens, MarvinPino, NataliaGiusti, SebastianHanan, MorBehm, MikaelaBartok, OsnatAshwal Fluss, ReutHerzog, MargaretaSchreyer, LuisaPapavasileiou, PanagiotisIvanov, AndranikÖhman, MarieRefojo, DamianKadener, SebastianRajewsky, Nikolauscircular RNAsnon-coding RNAbrainhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite their abundance, their function and expression in the nervous system are unknown. Therefore, we sequenced RNA from different brain regions, primary neurons, isolated synapses, as well as during neuronal differentiation. Using these and other available data, we discovered and analyzed thousands of neuronal human and mouse circRNAs. circRNAs were extraordinarily enriched in the mammalian brain, well conserved in sequence, often expressed as circRNAs in both human and mouse, and sometimes even detected in Drosophila brains. circRNAs were overall upregulated during neuronal differentiation, highly enriched in synapses, and often differentially expressed compared to their mRNA isoforms. circRNA expression correlated negatively with expression of the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated circRNA expression. Together, we provide a circRNA brain expression atlas and evidence for important circRNA functions and values as biomarkers.Fil: Rybak Wolf, Agnieszka. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Stottmeister, Christin. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Glažar, Petar. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Jens, Marvin. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Pino, Natalia. Max Planck Institute of Psychiatry; AlemaniaFil: Giusti, Sebastian. Max Planck Institute of Psychiatry; AlemaniaFil: Hanan, Mor. The Hebrew University of Jerusalem; IsraelFil: Behm, Mikaela. Stockholms Universitet; SueciaFil: Bartok, Osnat. The Hebrew University of Jerusalem; IsraelFil: Ashwal Fluss, Reut. The Hebrew University of Jerusalem; IsraelFil: Herzog, Margareta. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Schreyer, Luisa. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Papavasileiou, Panagiotis. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Ivanov, Andranik. Max-Delbrück Center for Molecular Medicine; AlemaniaFil: Öhman, Marie. Stockholms Universitet; SueciaFil: Refojo, Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina. Max Planck Institute of Psychiatry; AlemaniaFil: Kadener, Sebastian. The Hebrew University of Jerusalem; IsraelFil: Rajewsky, Nikolaus. Max-Delbrück Center for Molecular Medicine; AlemaniaCell Press2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85840Rybak Wolf, Agnieszka; Stottmeister, Christin; Glažar, Petar; Jens, Marvin; Pino, Natalia; et al.; Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed; Cell Press; Molecular Cell; 58; 5; 12-2014; 870-8851097-2765CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/doi/https://doi.org/10.1016/j.molcel.2015.05.027info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcel.2015.03.027info:eu-repo/semantics/altIdentifier/url/sciencedirect.com/science/article/pii/S109727651500218Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:13Zoai:ri.conicet.gov.ar:11336/85840instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:13.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
title Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
spellingShingle Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
Rybak Wolf, Agnieszka
circular RNAs
non-coding RNA
brain
title_short Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
title_full Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
title_fullStr Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
title_full_unstemmed Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
title_sort Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed
dc.creator.none.fl_str_mv Rybak Wolf, Agnieszka
Stottmeister, Christin
Glažar, Petar
Jens, Marvin
Pino, Natalia
Giusti, Sebastian
Hanan, Mor
Behm, Mikaela
Bartok, Osnat
Ashwal Fluss, Reut
Herzog, Margareta
Schreyer, Luisa
Papavasileiou, Panagiotis
Ivanov, Andranik
Öhman, Marie
Refojo, Damian
Kadener, Sebastian
Rajewsky, Nikolaus
author Rybak Wolf, Agnieszka
author_facet Rybak Wolf, Agnieszka
Stottmeister, Christin
Glažar, Petar
Jens, Marvin
Pino, Natalia
Giusti, Sebastian
Hanan, Mor
Behm, Mikaela
Bartok, Osnat
Ashwal Fluss, Reut
Herzog, Margareta
Schreyer, Luisa
Papavasileiou, Panagiotis
Ivanov, Andranik
Öhman, Marie
Refojo, Damian
Kadener, Sebastian
Rajewsky, Nikolaus
author_role author
author2 Stottmeister, Christin
Glažar, Petar
Jens, Marvin
Pino, Natalia
Giusti, Sebastian
Hanan, Mor
Behm, Mikaela
Bartok, Osnat
Ashwal Fluss, Reut
Herzog, Margareta
Schreyer, Luisa
Papavasileiou, Panagiotis
Ivanov, Andranik
Öhman, Marie
Refojo, Damian
Kadener, Sebastian
Rajewsky, Nikolaus
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv circular RNAs
non-coding RNA
brain
topic circular RNAs
non-coding RNA
brain
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite their abundance, their function and expression in the nervous system are unknown. Therefore, we sequenced RNA from different brain regions, primary neurons, isolated synapses, as well as during neuronal differentiation. Using these and other available data, we discovered and analyzed thousands of neuronal human and mouse circRNAs. circRNAs were extraordinarily enriched in the mammalian brain, well conserved in sequence, often expressed as circRNAs in both human and mouse, and sometimes even detected in Drosophila brains. circRNAs were overall upregulated during neuronal differentiation, highly enriched in synapses, and often differentially expressed compared to their mRNA isoforms. circRNA expression correlated negatively with expression of the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated circRNA expression. Together, we provide a circRNA brain expression atlas and evidence for important circRNA functions and values as biomarkers.
Fil: Rybak Wolf, Agnieszka. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Stottmeister, Christin. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Glažar, Petar. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Jens, Marvin. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Pino, Natalia. Max Planck Institute of Psychiatry; Alemania
Fil: Giusti, Sebastian. Max Planck Institute of Psychiatry; Alemania
Fil: Hanan, Mor. The Hebrew University of Jerusalem; Israel
Fil: Behm, Mikaela. Stockholms Universitet; Suecia
Fil: Bartok, Osnat. The Hebrew University of Jerusalem; Israel
Fil: Ashwal Fluss, Reut. The Hebrew University of Jerusalem; Israel
Fil: Herzog, Margareta. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Schreyer, Luisa. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Papavasileiou, Panagiotis. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Ivanov, Andranik. Max-Delbrück Center for Molecular Medicine; Alemania
Fil: Öhman, Marie. Stockholms Universitet; Suecia
Fil: Refojo, Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina. Max Planck Institute of Psychiatry; Alemania
Fil: Kadener, Sebastian. The Hebrew University of Jerusalem; Israel
Fil: Rajewsky, Nikolaus. Max-Delbrück Center for Molecular Medicine; Alemania
description Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite their abundance, their function and expression in the nervous system are unknown. Therefore, we sequenced RNA from different brain regions, primary neurons, isolated synapses, as well as during neuronal differentiation. Using these and other available data, we discovered and analyzed thousands of neuronal human and mouse circRNAs. circRNAs were extraordinarily enriched in the mammalian brain, well conserved in sequence, often expressed as circRNAs in both human and mouse, and sometimes even detected in Drosophila brains. circRNAs were overall upregulated during neuronal differentiation, highly enriched in synapses, and often differentially expressed compared to their mRNA isoforms. circRNA expression correlated negatively with expression of the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated circRNA expression. Together, we provide a circRNA brain expression atlas and evidence for important circRNA functions and values as biomarkers.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85840
Rybak Wolf, Agnieszka; Stottmeister, Christin; Glažar, Petar; Jens, Marvin; Pino, Natalia; et al.; Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed; Cell Press; Molecular Cell; 58; 5; 12-2014; 870-885
1097-2765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85840
identifier_str_mv Rybak Wolf, Agnieszka; Stottmeister, Christin; Glažar, Petar; Jens, Marvin; Pino, Natalia; et al.; Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed; Cell Press; Molecular Cell; 58; 5; 12-2014; 870-885
1097-2765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/doi/https://doi.org/10.1016/j.molcel.2015.05.027
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcel.2015.03.027
info:eu-repo/semantics/altIdentifier/url/sciencedirect.com/science/article/pii/S109727651500218X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269081300893696
score 13.13397

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