A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi

Autores
Camara, María de los Milagros; Bouvier, León Alberto; Reigada, Chantal; Di Girolamo, Fabio Augusto; Martínez Sayé, Melisa Soledad; Pereira, Claudio Alejandro
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases.
Fil: Camara, María de los Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina. Universidad Argentina de la Empresa. Facultad de Ingeniería y Ciencias Exactas. Instituto de Tecnología; Argentina
Fil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Reigada, Chantal. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Di Girolamo, Fabio Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Martínez Sayé, Melisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Materia
ENERGY METABOLISM
TRYPANOSOMATIDS
CHAGAS DISEASE
GLYCOSOMES
TRYPOMASTIGOTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47227
Acceder
id CONICETDig_a5abcb07173e98a2ccb7c5d496292a99
oai_identifier_str oai:ri.conicet.gov.ar:11336/47227
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruziCamara, María de los MilagrosBouvier, León AlbertoReigada, ChantalDi Girolamo, Fabio AugustoMartínez Sayé, Melisa SoledadPereira, Claudio AlejandroENERGY METABOLISMTRYPANOSOMATIDSCHAGAS DISEASEGLYCOSOMESTRYPOMASTIGOTEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases.Fil: Camara, María de los Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina. Universidad Argentina de la Empresa. Facultad de Ingeniería y Ciencias Exactas. Instituto de Tecnología; ArgentinaFil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Reigada, Chantal. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Di Girolamo, Fabio Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Martínez Sayé, Melisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaCzech Academy of Sciences. Institute of Parasitology2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47227Camara, María de los Milagros; Bouvier, León Alberto; Reigada, Chantal; Di Girolamo, Fabio Augusto; Martínez Sayé, Melisa Soledad; et al.; A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi; Czech Academy of Sciences. Institute of Parasitology; Folia Parasitologica; 64; 2-2017; 1-60015-56831803-6465CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://folia.paru.cas.cz/artkey/fol-201701-0006_a_novel_stage-specific_glycosomal_nucleoside_diphosphate_kinase_from_trypanosoma_cruzi.phpinfo:eu-repo/semantics/altIdentifier/doi/10.14411/fp.2017.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:12:33Zoai:ri.conicet.gov.ar:11336/47227instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:12:34.137CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
title A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
spellingShingle A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
Camara, María de los Milagros
ENERGY METABOLISM
TRYPANOSOMATIDS
CHAGAS DISEASE
GLYCOSOMES
TRYPOMASTIGOTES
title_short A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
title_full A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
title_fullStr A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
title_full_unstemmed A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
title_sort A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
dc.creator.none.fl_str_mv Camara, María de los Milagros
Bouvier, León Alberto
Reigada, Chantal
Di Girolamo, Fabio Augusto
Martínez Sayé, Melisa Soledad
Pereira, Claudio Alejandro
author Camara, María de los Milagros
author_facet Camara, María de los Milagros
Bouvier, León Alberto
Reigada, Chantal
Di Girolamo, Fabio Augusto
Martínez Sayé, Melisa Soledad
Pereira, Claudio Alejandro
author_role author
author2 Bouvier, León Alberto
Reigada, Chantal
Di Girolamo, Fabio Augusto
Martínez Sayé, Melisa Soledad
Pereira, Claudio Alejandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ENERGY METABOLISM
TRYPANOSOMATIDS
CHAGAS DISEASE
GLYCOSOMES
TRYPOMASTIGOTES
topic ENERGY METABOLISM
TRYPANOSOMATIDS
CHAGAS DISEASE
GLYCOSOMES
TRYPOMASTIGOTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases.
Fil: Camara, María de los Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina. Universidad Argentina de la Empresa. Facultad de Ingeniería y Ciencias Exactas. Instituto de Tecnología; Argentina
Fil: Bouvier, León Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Reigada, Chantal. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Di Girolamo, Fabio Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Martínez Sayé, Melisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
description Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47227
Camara, María de los Milagros; Bouvier, León Alberto; Reigada, Chantal; Di Girolamo, Fabio Augusto; Martínez Sayé, Melisa Soledad; et al.; A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi; Czech Academy of Sciences. Institute of Parasitology; Folia Parasitologica; 64; 2-2017; 1-6
0015-5683
1803-6465
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47227
identifier_str_mv Camara, María de los Milagros; Bouvier, León Alberto; Reigada, Chantal; Di Girolamo, Fabio Augusto; Martínez Sayé, Melisa Soledad; et al.; A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi; Czech Academy of Sciences. Institute of Parasitology; Folia Parasitologica; 64; 2-2017; 1-6
0015-5683
1803-6465
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://folia.paru.cas.cz/artkey/fol-201701-0006_a_novel_stage-specific_glycosomal_nucleoside_diphosphate_kinase_from_trypanosoma_cruzi.php
info:eu-repo/semantics/altIdentifier/doi/10.14411/fp.2017.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Czech Academy of Sciences. Institute of Parasitology
publisher.none.fl_str_mv Czech Academy of Sciences. Institute of Parasitology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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