Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model

Autores
D'anna, Maria Cecilia; Giorgi, Gisela; Roque, Marta Elena
Año de publicación
2011
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Duodenum, spleen and liver have a crucial role in iron balance on the whole organism and are the major sites of Ferroportin (FPN) expression. Specific regulations between FPN and hepcidin are responsible for changes seen in physiopathological conditions such as inflammation. We studied in vivo effects of turpentine oil-induced acute inflammation on FPN expression, and its relation with prohepcidin and iron mobilization. Immunohistochemical procedures were performed using rabbit anti-mouse FPN and prohepcidin antibodies with goat-labeled polymer-HRP anti-rabbit (DAB) as secondary antibody. Plasma and tissular iron were also studied. Our results showed a notable expression and redistribution of duodenal FPN to basolateral membrane in turpentine-treated mice, compared with supranuclear and the weak basolateral expression observed in healthy mice. Red pulp macrophages of healthy mice showed FPN-hemosiderin co-localization, compared with turpentine-treated mice which showed lack of FPN. In liver of healthy mice, FPN was seen in Kupffer cells, whereas in turpentine-treated mice decreased. In addition, we observed an increment of hepatic prohepcidin with a significant hypoferremia. Our findings demonstrated that acute inflammation induced a differential distribution of FPN, showing a cell type specific response. In macrophages, increased hepatic prohepcidin induced degradation of FPN, resulting in hypoferremia. In enterocytes, the redistribution observed of duodenal FPN reflects a different regulation in this tissue. The observed response of the proteins studied may be part of a cyclical pattern of systemic effects of acute inflammation on mouse tissue.
Fil: D'anna, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Universidad Nacional del Sur; Argentina
Materia
ENTEROCYTES
IRON
MACROPHAGES
MOBILIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66731
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66731
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation modelEstudios inmunohistoquímicos en duodeno, bazo e hígado de ratón: Distribución de ferroportina y prohepcidina en un modelo de inflamaciónD'anna, Maria CeciliaGiorgi, GiselaRoque, Marta ElenaENTEROCYTESIRONMACROPHAGESMOBILIZATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Duodenum, spleen and liver have a crucial role in iron balance on the whole organism and are the major sites of Ferroportin (FPN) expression. Specific regulations between FPN and hepcidin are responsible for changes seen in physiopathological conditions such as inflammation. We studied in vivo effects of turpentine oil-induced acute inflammation on FPN expression, and its relation with prohepcidin and iron mobilization. Immunohistochemical procedures were performed using rabbit anti-mouse FPN and prohepcidin antibodies with goat-labeled polymer-HRP anti-rabbit (DAB) as secondary antibody. Plasma and tissular iron were also studied. Our results showed a notable expression and redistribution of duodenal FPN to basolateral membrane in turpentine-treated mice, compared with supranuclear and the weak basolateral expression observed in healthy mice. Red pulp macrophages of healthy mice showed FPN-hemosiderin co-localization, compared with turpentine-treated mice which showed lack of FPN. In liver of healthy mice, FPN was seen in Kupffer cells, whereas in turpentine-treated mice decreased. In addition, we observed an increment of hepatic prohepcidin with a significant hypoferremia. Our findings demonstrated that acute inflammation induced a differential distribution of FPN, showing a cell type specific response. In macrophages, increased hepatic prohepcidin induced degradation of FPN, resulting in hypoferremia. In enterocytes, the redistribution observed of duodenal FPN reflects a different regulation in this tissue. The observed response of the proteins studied may be part of a cyclical pattern of systemic effects of acute inflammation on mouse tissue.Fil: D'anna, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Roque, Marta Elena. Universidad Nacional del Sur; ArgentinaSociedad Chilena de Anatomía2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66731D'anna, Maria Cecilia; Giorgi, Gisela; Roque, Marta Elena; Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model; Sociedad Chilena de Anatomía; International Journal of Morphology; 29; 3; 9-2011; 747-7530717-93670717-9502CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/doi/10.4067/S0717-95022011000300014info:eu-repo/semantics/altIdentifier/url/https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300014&lng=en&nrm=iso&tlng=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:04Zoai:ri.conicet.gov.ar:11336/66731instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:04.934CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
Estudios inmunohistoquímicos en duodeno, bazo e hígado de ratón: Distribución de ferroportina y prohepcidina en un modelo de inflamación
title Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
spellingShingle Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
D'anna, Maria Cecilia
ENTEROCYTES
IRON
MACROPHAGES
MOBILIZATION
title_short Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
title_full Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
title_fullStr Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
title_full_unstemmed Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
title_sort Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model
dc.creator.none.fl_str_mv D'anna, Maria Cecilia
Giorgi, Gisela
Roque, Marta Elena
author D'anna, Maria Cecilia
author_facet D'anna, Maria Cecilia
Giorgi, Gisela
Roque, Marta Elena
author_role author
author2 Giorgi, Gisela
Roque, Marta Elena
author2_role author
author
dc.subject.none.fl_str_mv ENTEROCYTES
IRON
MACROPHAGES
MOBILIZATION
topic ENTEROCYTES
IRON
MACROPHAGES
MOBILIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Duodenum, spleen and liver have a crucial role in iron balance on the whole organism and are the major sites of Ferroportin (FPN) expression. Specific regulations between FPN and hepcidin are responsible for changes seen in physiopathological conditions such as inflammation. We studied in vivo effects of turpentine oil-induced acute inflammation on FPN expression, and its relation with prohepcidin and iron mobilization. Immunohistochemical procedures were performed using rabbit anti-mouse FPN and prohepcidin antibodies with goat-labeled polymer-HRP anti-rabbit (DAB) as secondary antibody. Plasma and tissular iron were also studied. Our results showed a notable expression and redistribution of duodenal FPN to basolateral membrane in turpentine-treated mice, compared with supranuclear and the weak basolateral expression observed in healthy mice. Red pulp macrophages of healthy mice showed FPN-hemosiderin co-localization, compared with turpentine-treated mice which showed lack of FPN. In liver of healthy mice, FPN was seen in Kupffer cells, whereas in turpentine-treated mice decreased. In addition, we observed an increment of hepatic prohepcidin with a significant hypoferremia. Our findings demonstrated that acute inflammation induced a differential distribution of FPN, showing a cell type specific response. In macrophages, increased hepatic prohepcidin induced degradation of FPN, resulting in hypoferremia. In enterocytes, the redistribution observed of duodenal FPN reflects a different regulation in this tissue. The observed response of the proteins studied may be part of a cyclical pattern of systemic effects of acute inflammation on mouse tissue.
Fil: D'anna, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Universidad Nacional del Sur; Argentina
description Duodenum, spleen and liver have a crucial role in iron balance on the whole organism and are the major sites of Ferroportin (FPN) expression. Specific regulations between FPN and hepcidin are responsible for changes seen in physiopathological conditions such as inflammation. We studied in vivo effects of turpentine oil-induced acute inflammation on FPN expression, and its relation with prohepcidin and iron mobilization. Immunohistochemical procedures were performed using rabbit anti-mouse FPN and prohepcidin antibodies with goat-labeled polymer-HRP anti-rabbit (DAB) as secondary antibody. Plasma and tissular iron were also studied. Our results showed a notable expression and redistribution of duodenal FPN to basolateral membrane in turpentine-treated mice, compared with supranuclear and the weak basolateral expression observed in healthy mice. Red pulp macrophages of healthy mice showed FPN-hemosiderin co-localization, compared with turpentine-treated mice which showed lack of FPN. In liver of healthy mice, FPN was seen in Kupffer cells, whereas in turpentine-treated mice decreased. In addition, we observed an increment of hepatic prohepcidin with a significant hypoferremia. Our findings demonstrated that acute inflammation induced a differential distribution of FPN, showing a cell type specific response. In macrophages, increased hepatic prohepcidin induced degradation of FPN, resulting in hypoferremia. In enterocytes, the redistribution observed of duodenal FPN reflects a different regulation in this tissue. The observed response of the proteins studied may be part of a cyclical pattern of systemic effects of acute inflammation on mouse tissue.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66731
D'anna, Maria Cecilia; Giorgi, Gisela; Roque, Marta Elena; Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model; Sociedad Chilena de Anatomía; International Journal of Morphology; 29; 3; 9-2011; 747-753
0717-9367
0717-9502
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66731
identifier_str_mv D'anna, Maria Cecilia; Giorgi, Gisela; Roque, Marta Elena; Immunohistochemical studies on duodenum, spleen and liver in mice: Distribution of ferroportin and prohepcidin in an inflammation model; Sociedad Chilena de Anatomía; International Journal of Morphology; 29; 3; 9-2011; 747-753
0717-9367
0717-9502
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4067/S0717-95022011000300014
info:eu-repo/semantics/altIdentifier/url/https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300014&lng=en&nrm=iso&tlng=en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Chilena de Anatomía
publisher.none.fl_str_mv Sociedad Chilena de Anatomía
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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