Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights

Autores
Bocanegra, M. P.; Lobartini, Juan Carlos; Orioli, Gustavo Adolfo
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mobilization of iron (Fe) chelated by humic acids (HA) of low (HA10,000) and high molecular weight (HA100,000) fractions and its uptake by plants were investigated in growth experiments with sunflower seedlings. The iron chelates (labeled with 59Fe) contained in dialysis bags (mw. cutoff=3500) were placed in minus iron Hoagland solutions as the Fe source and at the same time fulvic acid (FA), EDTA, and low and high molecular weight HA fractions were added in the solutions as mobilizators. Characterization of FA, HA10,000, and HA100,000 were performed by infrared spectroscopy and chemical analysis, e.g., total acidity, COOH, and phenolic‐OH content. Roots and leaves were harvested, dried, and ground for Fe activity determination. Iron contents and pH in the nutrient solutions were measured before and after treatments. The supply of Fe to the plants was apparently sufficient, because no Fe deficiency has been detected in the test plants but during the whole absorption period, the pH of the nutrient solution was about 4.5. The Fe contents in leaves indicated that part of the Fe was rapidly transported from roots to leaves. Judging from the Fe contents in leaves, it was assumed that the small size HA10,000 and EDTA were the most efficient in affecting transport of Fe from root to leaf tissue. FA, HA10,000, and especially HA100,000 were unable to penetrate the dialysis bags and, hence, were effective in Fe mobilization only after the Fe, dissociated from the Fe‐HA chelate, has passed the dialysis membrane into the nutrient solutions. In contrast, the small size EDTA was expected to have penetrated the dialysis bags, permitting mobilization of chelated Fe by ligand exchange inside the bags, and transporting the Fe to the roots. The results suggested that the humic substances used in this study were able to form with the Fe3+ ion complexes that maintained the iron available to the sunflower plants. In the chemical form of Fe.L, where L was FA o HA, the iron within the bags or in solution or in the roots free space, was available for exchange reactions with the natural sunflower plant chelators for its transport to the leaves.
Fil: Bocanegra, M. P.. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Lobartini, Juan Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Orioli, Gustavo Adolfo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
Humic Acids
Iron
Absorbtion
Mobilization
Complexes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34469

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oai_identifier_str oai:ri.conicet.gov.ar:11336/34469
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Plant Uptake of Iron Chelated by Humic Acids of Different Molecular WeightsBocanegra, M. P.Lobartini, Juan CarlosOrioli, Gustavo AdolfoHumic AcidsIronAbsorbtionMobilizationComplexeshttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Mobilization of iron (Fe) chelated by humic acids (HA) of low (HA10,000) and high molecular weight (HA100,000) fractions and its uptake by plants were investigated in growth experiments with sunflower seedlings. The iron chelates (labeled with 59Fe) contained in dialysis bags (mw. cutoff=3500) were placed in minus iron Hoagland solutions as the Fe source and at the same time fulvic acid (FA), EDTA, and low and high molecular weight HA fractions were added in the solutions as mobilizators. Characterization of FA, HA10,000, and HA100,000 were performed by infrared spectroscopy and chemical analysis, e.g., total acidity, COOH, and phenolic‐OH content. Roots and leaves were harvested, dried, and ground for Fe activity determination. Iron contents and pH in the nutrient solutions were measured before and after treatments. The supply of Fe to the plants was apparently sufficient, because no Fe deficiency has been detected in the test plants but during the whole absorption period, the pH of the nutrient solution was about 4.5. The Fe contents in leaves indicated that part of the Fe was rapidly transported from roots to leaves. Judging from the Fe contents in leaves, it was assumed that the small size HA10,000 and EDTA were the most efficient in affecting transport of Fe from root to leaf tissue. FA, HA10,000, and especially HA100,000 were unable to penetrate the dialysis bags and, hence, were effective in Fe mobilization only after the Fe, dissociated from the Fe‐HA chelate, has passed the dialysis membrane into the nutrient solutions. In contrast, the small size EDTA was expected to have penetrated the dialysis bags, permitting mobilization of chelated Fe by ligand exchange inside the bags, and transporting the Fe to the roots. The results suggested that the humic substances used in this study were able to form with the Fe3+ ion complexes that maintained the iron available to the sunflower plants. In the chemical form of Fe.L, where L was FA o HA, the iron within the bags or in solution or in the roots free space, was available for exchange reactions with the natural sunflower plant chelators for its transport to the leaves.Fil: Bocanegra, M. P.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Lobartini, Juan Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Orioli, Gustavo Adolfo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaTaylor & Francis2006-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34469Bocanegra, M. P.; Lobartini, Juan Carlos; Orioli, Gustavo Adolfo; Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights; Taylor & Francis; Communications in Soil Science and Plant Analysis; 37; 1-2; 2-2006; 239-2480010-3624CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/00103620500408779info:eu-repo/semantics/altIdentifier/doi/10.1080/00103620500408779info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:32Zoai:ri.conicet.gov.ar:11336/34469instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:32.4CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
title Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
spellingShingle Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
Bocanegra, M. P.
Humic Acids
Iron
Absorbtion
Mobilization
Complexes
title_short Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
title_full Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
title_fullStr Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
title_full_unstemmed Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
title_sort Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights
dc.creator.none.fl_str_mv Bocanegra, M. P.
Lobartini, Juan Carlos
Orioli, Gustavo Adolfo
author Bocanegra, M. P.
author_facet Bocanegra, M. P.
Lobartini, Juan Carlos
Orioli, Gustavo Adolfo
author_role author
author2 Lobartini, Juan Carlos
Orioli, Gustavo Adolfo
author2_role author
author
dc.subject.none.fl_str_mv Humic Acids
Iron
Absorbtion
Mobilization
Complexes
topic Humic Acids
Iron
Absorbtion
Mobilization
Complexes
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Mobilization of iron (Fe) chelated by humic acids (HA) of low (HA10,000) and high molecular weight (HA100,000) fractions and its uptake by plants were investigated in growth experiments with sunflower seedlings. The iron chelates (labeled with 59Fe) contained in dialysis bags (mw. cutoff=3500) were placed in minus iron Hoagland solutions as the Fe source and at the same time fulvic acid (FA), EDTA, and low and high molecular weight HA fractions were added in the solutions as mobilizators. Characterization of FA, HA10,000, and HA100,000 were performed by infrared spectroscopy and chemical analysis, e.g., total acidity, COOH, and phenolic‐OH content. Roots and leaves were harvested, dried, and ground for Fe activity determination. Iron contents and pH in the nutrient solutions were measured before and after treatments. The supply of Fe to the plants was apparently sufficient, because no Fe deficiency has been detected in the test plants but during the whole absorption period, the pH of the nutrient solution was about 4.5. The Fe contents in leaves indicated that part of the Fe was rapidly transported from roots to leaves. Judging from the Fe contents in leaves, it was assumed that the small size HA10,000 and EDTA were the most efficient in affecting transport of Fe from root to leaf tissue. FA, HA10,000, and especially HA100,000 were unable to penetrate the dialysis bags and, hence, were effective in Fe mobilization only after the Fe, dissociated from the Fe‐HA chelate, has passed the dialysis membrane into the nutrient solutions. In contrast, the small size EDTA was expected to have penetrated the dialysis bags, permitting mobilization of chelated Fe by ligand exchange inside the bags, and transporting the Fe to the roots. The results suggested that the humic substances used in this study were able to form with the Fe3+ ion complexes that maintained the iron available to the sunflower plants. In the chemical form of Fe.L, where L was FA o HA, the iron within the bags or in solution or in the roots free space, was available for exchange reactions with the natural sunflower plant chelators for its transport to the leaves.
Fil: Bocanegra, M. P.. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Lobartini, Juan Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Orioli, Gustavo Adolfo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Mobilization of iron (Fe) chelated by humic acids (HA) of low (HA10,000) and high molecular weight (HA100,000) fractions and its uptake by plants were investigated in growth experiments with sunflower seedlings. The iron chelates (labeled with 59Fe) contained in dialysis bags (mw. cutoff=3500) were placed in minus iron Hoagland solutions as the Fe source and at the same time fulvic acid (FA), EDTA, and low and high molecular weight HA fractions were added in the solutions as mobilizators. Characterization of FA, HA10,000, and HA100,000 were performed by infrared spectroscopy and chemical analysis, e.g., total acidity, COOH, and phenolic‐OH content. Roots and leaves were harvested, dried, and ground for Fe activity determination. Iron contents and pH in the nutrient solutions were measured before and after treatments. The supply of Fe to the plants was apparently sufficient, because no Fe deficiency has been detected in the test plants but during the whole absorption period, the pH of the nutrient solution was about 4.5. The Fe contents in leaves indicated that part of the Fe was rapidly transported from roots to leaves. Judging from the Fe contents in leaves, it was assumed that the small size HA10,000 and EDTA were the most efficient in affecting transport of Fe from root to leaf tissue. FA, HA10,000, and especially HA100,000 were unable to penetrate the dialysis bags and, hence, were effective in Fe mobilization only after the Fe, dissociated from the Fe‐HA chelate, has passed the dialysis membrane into the nutrient solutions. In contrast, the small size EDTA was expected to have penetrated the dialysis bags, permitting mobilization of chelated Fe by ligand exchange inside the bags, and transporting the Fe to the roots. The results suggested that the humic substances used in this study were able to form with the Fe3+ ion complexes that maintained the iron available to the sunflower plants. In the chemical form of Fe.L, where L was FA o HA, the iron within the bags or in solution or in the roots free space, was available for exchange reactions with the natural sunflower plant chelators for its transport to the leaves.
publishDate 2006
dc.date.none.fl_str_mv 2006-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34469
Bocanegra, M. P.; Lobartini, Juan Carlos; Orioli, Gustavo Adolfo; Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights; Taylor & Francis; Communications in Soil Science and Plant Analysis; 37; 1-2; 2-2006; 239-248
0010-3624
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34469
identifier_str_mv Bocanegra, M. P.; Lobartini, Juan Carlos; Orioli, Gustavo Adolfo; Plant Uptake of Iron Chelated by Humic Acids of Different Molecular Weights; Taylor & Francis; Communications in Soil Science and Plant Analysis; 37; 1-2; 2-2006; 239-248
0010-3624
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/00103620500408779
info:eu-repo/semantics/altIdentifier/doi/10.1080/00103620500408779
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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