Cryopreservation of in vitro-produced ovine embryos

Autores
Martínez, A.G.; Valcárcel, A.; Furnus, Cecilia Cristina; de Matos, D.G.; Iorio, G.; de las Heras, M.A.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experiment, ovine embryos cryopreserved in ethylene glycol using 3 steps (E-3S) showed a higher hatching rate and nuclei number than freezing with glycerol in 3 steps (G-3S) (40.0% versus 20.0% and 135.4 ± 20.7 versus 118.5 ± 19.5, respectively). In a second experiment, vitrification with ethylene glycol + Ficoll 70 + sucrose (EFS) recorded a higher hatching rate and nuclei numbers than vitrification with propylene glycol + glycerol (Pg + Gly) (51.1% versus 31.1% and 133.8 ± 36.8 versus 113.5 ± 22.0, respectively). In a third phase, vitrification of embryos with ethylene glycol + glycerol (Eg + Gly) resulted in higher development and hatching rates and nuclei number than EFS (87.3% versus 65.4%; 76.4% versus 54.5% and 135.7 ± 34.5 versus 113.1 ± 14.1, respectively). In a fourth experiment, fresh in vivo embryos produced a higher lambing rate (67.8%) than the other methods, and E-3S in vitro resulted in lower lambing rates (23.0%). E-3S in vivo, Eg + Gly in vivo, fresh in vitro and Eg + Gly in vitro recorded similar lambing rates (42.8, 37.5, 37.5 and 26.6%, respectively). Ewes receiving in vitro-produced ovine embryos resulted in higher assisted births and perinatal losses than those receiving in vivo-produced embryos (3.6% versus 16.9% and 1.2% versus 10.1%, respectively). Results demonstrate that the transfer of in vitro-produced embryos vitrified with Eg + Gly could have wide application, if the negative side effects of the produced offspring can be managed.
Fil: Martínez, A.G.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: Valcárcel, A.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: Furnus, Cecilia Cristina. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: de Matos, D.G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: Iorio, G.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: de las Heras, M.A.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Materia
In Vitro Survival
Lambing
Transfer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82439
Acceder
id CONICETDig_a4acc3f591c608998b65e138447afabd
oai_identifier_str oai:ri.conicet.gov.ar:11336/82439
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cryopreservation of in vitro-produced ovine embryosMartínez, A.G.Valcárcel, A.Furnus, Cecilia Cristinade Matos, D.G.Iorio, G.de las Heras, M.A.In Vitro SurvivalLambingTransferhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experiment, ovine embryos cryopreserved in ethylene glycol using 3 steps (E-3S) showed a higher hatching rate and nuclei number than freezing with glycerol in 3 steps (G-3S) (40.0% versus 20.0% and 135.4 ± 20.7 versus 118.5 ± 19.5, respectively). In a second experiment, vitrification with ethylene glycol + Ficoll 70 + sucrose (EFS) recorded a higher hatching rate and nuclei numbers than vitrification with propylene glycol + glycerol (Pg + Gly) (51.1% versus 31.1% and 133.8 ± 36.8 versus 113.5 ± 22.0, respectively). In a third phase, vitrification of embryos with ethylene glycol + glycerol (Eg + Gly) resulted in higher development and hatching rates and nuclei number than EFS (87.3% versus 65.4%; 76.4% versus 54.5% and 135.7 ± 34.5 versus 113.1 ± 14.1, respectively). In a fourth experiment, fresh in vivo embryos produced a higher lambing rate (67.8%) than the other methods, and E-3S in vitro resulted in lower lambing rates (23.0%). E-3S in vivo, Eg + Gly in vivo, fresh in vitro and Eg + Gly in vitro recorded similar lambing rates (42.8, 37.5, 37.5 and 26.6%, respectively). Ewes receiving in vitro-produced ovine embryos resulted in higher assisted births and perinatal losses than those receiving in vivo-produced embryos (3.6% versus 16.9% and 1.2% versus 10.1%, respectively). Results demonstrate that the transfer of in vitro-produced embryos vitrified with Eg + Gly could have wide application, if the negative side effects of the produced offspring can be managed.Fil: Martínez, A.G.. Centro de Investigaciones Reproductivas Perez Companc; ArgentinaFil: Valcárcel, A.. Centro de Investigaciones Reproductivas Perez Companc; ArgentinaFil: Furnus, Cecilia Cristina. Centro de Investigaciones Reproductivas Perez Companc; ArgentinaFil: de Matos, D.G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Centro de Investigaciones Reproductivas Perez Companc; ArgentinaFil: Iorio, G.. Centro de Investigaciones Reproductivas Perez Companc; ArgentinaFil: de las Heras, M.A.. Centro de Investigaciones Reproductivas Perez Companc; ArgentinaElsevier Science2006-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82439Martínez, A.G.; Valcárcel, A.; Furnus, Cecilia Cristina; de Matos, D.G.; Iorio, G.; et al.; Cryopreservation of in vitro-produced ovine embryos; Elsevier Science; Journal of Small Ruminant Research; 63; 3; 6-2006; 288-2960921-4488CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2005.03.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0921448805000945info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:36:12Zoai:ri.conicet.gov.ar:11336/82439instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:36:12.932CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cryopreservation of in vitro-produced ovine embryos
title Cryopreservation of in vitro-produced ovine embryos
spellingShingle Cryopreservation of in vitro-produced ovine embryos
Martínez, A.G.
In Vitro Survival
Lambing
Transfer
title_short Cryopreservation of in vitro-produced ovine embryos
title_full Cryopreservation of in vitro-produced ovine embryos
title_fullStr Cryopreservation of in vitro-produced ovine embryos
title_full_unstemmed Cryopreservation of in vitro-produced ovine embryos
title_sort Cryopreservation of in vitro-produced ovine embryos
dc.creator.none.fl_str_mv Martínez, A.G.
Valcárcel, A.
Furnus, Cecilia Cristina
de Matos, D.G.
Iorio, G.
de las Heras, M.A.
author Martínez, A.G.
author_facet Martínez, A.G.
Valcárcel, A.
Furnus, Cecilia Cristina
de Matos, D.G.
Iorio, G.
de las Heras, M.A.
author_role author
author2 Valcárcel, A.
Furnus, Cecilia Cristina
de Matos, D.G.
Iorio, G.
de las Heras, M.A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv In Vitro Survival
Lambing
Transfer
topic In Vitro Survival
Lambing
Transfer
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experiment, ovine embryos cryopreserved in ethylene glycol using 3 steps (E-3S) showed a higher hatching rate and nuclei number than freezing with glycerol in 3 steps (G-3S) (40.0% versus 20.0% and 135.4 ± 20.7 versus 118.5 ± 19.5, respectively). In a second experiment, vitrification with ethylene glycol + Ficoll 70 + sucrose (EFS) recorded a higher hatching rate and nuclei numbers than vitrification with propylene glycol + glycerol (Pg + Gly) (51.1% versus 31.1% and 133.8 ± 36.8 versus 113.5 ± 22.0, respectively). In a third phase, vitrification of embryos with ethylene glycol + glycerol (Eg + Gly) resulted in higher development and hatching rates and nuclei number than EFS (87.3% versus 65.4%; 76.4% versus 54.5% and 135.7 ± 34.5 versus 113.1 ± 14.1, respectively). In a fourth experiment, fresh in vivo embryos produced a higher lambing rate (67.8%) than the other methods, and E-3S in vitro resulted in lower lambing rates (23.0%). E-3S in vivo, Eg + Gly in vivo, fresh in vitro and Eg + Gly in vitro recorded similar lambing rates (42.8, 37.5, 37.5 and 26.6%, respectively). Ewes receiving in vitro-produced ovine embryos resulted in higher assisted births and perinatal losses than those receiving in vivo-produced embryos (3.6% versus 16.9% and 1.2% versus 10.1%, respectively). Results demonstrate that the transfer of in vitro-produced embryos vitrified with Eg + Gly could have wide application, if the negative side effects of the produced offspring can be managed.
Fil: Martínez, A.G.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: Valcárcel, A.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: Furnus, Cecilia Cristina. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: de Matos, D.G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: Iorio, G.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
Fil: de las Heras, M.A.. Centro de Investigaciones Reproductivas Perez Companc; Argentina
description The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experiment, ovine embryos cryopreserved in ethylene glycol using 3 steps (E-3S) showed a higher hatching rate and nuclei number than freezing with glycerol in 3 steps (G-3S) (40.0% versus 20.0% and 135.4 ± 20.7 versus 118.5 ± 19.5, respectively). In a second experiment, vitrification with ethylene glycol + Ficoll 70 + sucrose (EFS) recorded a higher hatching rate and nuclei numbers than vitrification with propylene glycol + glycerol (Pg + Gly) (51.1% versus 31.1% and 133.8 ± 36.8 versus 113.5 ± 22.0, respectively). In a third phase, vitrification of embryos with ethylene glycol + glycerol (Eg + Gly) resulted in higher development and hatching rates and nuclei number than EFS (87.3% versus 65.4%; 76.4% versus 54.5% and 135.7 ± 34.5 versus 113.1 ± 14.1, respectively). In a fourth experiment, fresh in vivo embryos produced a higher lambing rate (67.8%) than the other methods, and E-3S in vitro resulted in lower lambing rates (23.0%). E-3S in vivo, Eg + Gly in vivo, fresh in vitro and Eg + Gly in vitro recorded similar lambing rates (42.8, 37.5, 37.5 and 26.6%, respectively). Ewes receiving in vitro-produced ovine embryos resulted in higher assisted births and perinatal losses than those receiving in vivo-produced embryos (3.6% versus 16.9% and 1.2% versus 10.1%, respectively). Results demonstrate that the transfer of in vitro-produced embryos vitrified with Eg + Gly could have wide application, if the negative side effects of the produced offspring can be managed.
publishDate 2006
dc.date.none.fl_str_mv 2006-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82439
Martínez, A.G.; Valcárcel, A.; Furnus, Cecilia Cristina; de Matos, D.G.; Iorio, G.; et al.; Cryopreservation of in vitro-produced ovine embryos; Elsevier Science; Journal of Small Ruminant Research; 63; 3; 6-2006; 288-296
0921-4488
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82439
identifier_str_mv Martínez, A.G.; Valcárcel, A.; Furnus, Cecilia Cristina; de Matos, D.G.; Iorio, G.; et al.; Cryopreservation of in vitro-produced ovine embryos; Elsevier Science; Journal of Small Ruminant Research; 63; 3; 6-2006; 288-296
0921-4488
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2005.03.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0921448805000945
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848597244204285952
score 13.24909

Publicaciones similares