In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis

Autores
Aiassa, Virginia; Barnes, Ana Isabel; Albesa, Inés
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus epidermidis is a common pathogen in medical device-associated infections. Its major pathogenic factor is the ability to form adherent biofilms. In this work three strains of S. epidermidis isolated from infected catheters was chosen with the objective to investigate the effect of D-glucosamine (D-Glu) on reactive oxygen species (ROS) production, adhesion and biofilm formation. We used the assay of chemiluminescence and nitroblue tetrazolium reduction to determinate the ROS production by planktonic S. epidermidis and microtiter plate assay to quantify in vitro biofilm formation. D-Glu generated a dose-dependent increase in ROS in planktonic cells with maximum stimuli at a concentration of 0.05 mM, and reduced the adhesion and biofilm formation. Whereas, glucose showed an anti-oxidative stress action and promoted the adhesion and growth of biofilm. This study suggests a potential application of D-Glu against infections associated with indwelling medical devices, since the oxidative stress caused by this hexosamine in planktonic S. epidermidis contributed to reduce the biofilm formation.
Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Barnes, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Materia
Staphylococcus epidermidis
D-glucosamine
Reactive oxygen species
Biofilm
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195610
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195610
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidisAiassa, VirginiaBarnes, Ana IsabelAlbesa, InésStaphylococcus epidermidisD-glucosamineReactive oxygen speciesBiofilmhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Staphylococcus epidermidis is a common pathogen in medical device-associated infections. Its major pathogenic factor is the ability to form adherent biofilms. In this work three strains of S. epidermidis isolated from infected catheters was chosen with the objective to investigate the effect of D-glucosamine (D-Glu) on reactive oxygen species (ROS) production, adhesion and biofilm formation. We used the assay of chemiluminescence and nitroblue tetrazolium reduction to determinate the ROS production by planktonic S. epidermidis and microtiter plate assay to quantify in vitro biofilm formation. D-Glu generated a dose-dependent increase in ROS in planktonic cells with maximum stimuli at a concentration of 0.05 mM, and reduced the adhesion and biofilm formation. Whereas, glucose showed an anti-oxidative stress action and promoted the adhesion and growth of biofilm. This study suggests a potential application of D-Glu against infections associated with indwelling medical devices, since the oxidative stress caused by this hexosamine in planktonic S. epidermidis contributed to reduce the biofilm formation.Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Barnes, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaAsociación Argentina de Microbiología2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195610Aiassa, Virginia; Barnes, Ana Isabel; Albesa, Inés; In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 44; 1; 1-2012; 16-200325-75411851-7617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/22610292/info:eu-repo/semantics/altIdentifier/doi/10.1590/S0325-75412012000100004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:20Zoai:ri.conicet.gov.ar:11336/195610instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:20.487CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
title In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
spellingShingle In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
Aiassa, Virginia
Staphylococcus epidermidis
D-glucosamine
Reactive oxygen species
Biofilm
title_short In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
title_full In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
title_fullStr In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
title_full_unstemmed In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
title_sort In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis
dc.creator.none.fl_str_mv Aiassa, Virginia
Barnes, Ana Isabel
Albesa, Inés
author Aiassa, Virginia
author_facet Aiassa, Virginia
Barnes, Ana Isabel
Albesa, Inés
author_role author
author2 Barnes, Ana Isabel
Albesa, Inés
author2_role author
author
dc.subject.none.fl_str_mv Staphylococcus epidermidis
D-glucosamine
Reactive oxygen species
Biofilm
topic Staphylococcus epidermidis
D-glucosamine
Reactive oxygen species
Biofilm
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Staphylococcus epidermidis is a common pathogen in medical device-associated infections. Its major pathogenic factor is the ability to form adherent biofilms. In this work three strains of S. epidermidis isolated from infected catheters was chosen with the objective to investigate the effect of D-glucosamine (D-Glu) on reactive oxygen species (ROS) production, adhesion and biofilm formation. We used the assay of chemiluminescence and nitroblue tetrazolium reduction to determinate the ROS production by planktonic S. epidermidis and microtiter plate assay to quantify in vitro biofilm formation. D-Glu generated a dose-dependent increase in ROS in planktonic cells with maximum stimuli at a concentration of 0.05 mM, and reduced the adhesion and biofilm formation. Whereas, glucose showed an anti-oxidative stress action and promoted the adhesion and growth of biofilm. This study suggests a potential application of D-Glu against infections associated with indwelling medical devices, since the oxidative stress caused by this hexosamine in planktonic S. epidermidis contributed to reduce the biofilm formation.
Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Barnes, Ana Isabel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
description Staphylococcus epidermidis is a common pathogen in medical device-associated infections. Its major pathogenic factor is the ability to form adherent biofilms. In this work three strains of S. epidermidis isolated from infected catheters was chosen with the objective to investigate the effect of D-glucosamine (D-Glu) on reactive oxygen species (ROS) production, adhesion and biofilm formation. We used the assay of chemiluminescence and nitroblue tetrazolium reduction to determinate the ROS production by planktonic S. epidermidis and microtiter plate assay to quantify in vitro biofilm formation. D-Glu generated a dose-dependent increase in ROS in planktonic cells with maximum stimuli at a concentration of 0.05 mM, and reduced the adhesion and biofilm formation. Whereas, glucose showed an anti-oxidative stress action and promoted the adhesion and growth of biofilm. This study suggests a potential application of D-Glu against infections associated with indwelling medical devices, since the oxidative stress caused by this hexosamine in planktonic S. epidermidis contributed to reduce the biofilm formation.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195610
Aiassa, Virginia; Barnes, Ana Isabel; Albesa, Inés; In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 44; 1; 1-2012; 16-20
0325-7541
1851-7617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195610
identifier_str_mv Aiassa, Virginia; Barnes, Ana Isabel; Albesa, Inés; In vitro oxidant effects of D-glucosamine reduced adhesion and biofilm formation of Staphylococcus epidermidis; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 44; 1; 1-2012; 16-20
0325-7541
1851-7617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/22610292/
info:eu-repo/semantics/altIdentifier/doi/10.1590/S0325-75412012000100004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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