Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid

Autores
Laserna, María Paula; López, C. G.; Aulicino, Mónica Beatriz; Maddonni, Gustavo Angel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies have documented that transgene introduction may alter the phenotypic expression of several traits (e.g., biomass production, grain yield). We hypothesized that genetic diversity could influence the phenotypic variation among hybrids of a same genetic background and also among plants of a hybrid. The objectives of this preliminary study were: (i) to quantify the genetic diversity between the non-transgenic (DK747) and transgenic versions (DK747MG, DK747RR and DK747MGRR) of a single-cross maize hybrid and among plants of each version, (ii) to observe the distribution of genetic diversity along the genome and (iii) to explore relationships between phenotypic variability and genetic diversity. Hybrids were cultivated at field conditions during two growing seasons and plants of each hybrid with high, intermediate and low biomass at physiological maturity were selected to perform a study of single nucleotide polymorphisms (SNP). Genetic diversity among plants of each version was greater than among versions and both sources of variation were significant (ΦST = 0.45, P < 0.01). Genetic diversity of the non-transgenic DK747 was higher than those of the transgenic versions, probably reflecting the conventional breeding history of these iso-hybrids. Similarity coefficients indicate that the most homogeneous group was that composed by plants of DK747MGRR. A Fisher's exact test together with a principal component analysis identified certain SNPs related to the contrasting plant biomass of DK747, 747MG and DK747RR. Caution should be taken with these results, because of the small sample size for SNPs study and the narrow set of tested hybrids.
Fil: Laserna, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: López, C. G.. Universidad Nacional de Lomas de Zamora; Argentina
Fil: Aulicino, Mónica Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Instituto Fitotécnico de "Santa Catalina"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maddonni, Gustavo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Materia
Zea Mays
Single Nucleotide Polymorphism
Genetic Diversity
Non-Transgenic Hybrid
Transgenic Hybridation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17447
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17447
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybridLaserna, María PaulaLópez, C. G.Aulicino, Mónica BeatrizMaddonni, Gustavo AngelZea MaysSingle Nucleotide PolymorphismGenetic DiversityNon-Transgenic HybridTransgenic Hybridationhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Previous studies have documented that transgene introduction may alter the phenotypic expression of several traits (e.g., biomass production, grain yield). We hypothesized that genetic diversity could influence the phenotypic variation among hybrids of a same genetic background and also among plants of a hybrid. The objectives of this preliminary study were: (i) to quantify the genetic diversity between the non-transgenic (DK747) and transgenic versions (DK747MG, DK747RR and DK747MGRR) of a single-cross maize hybrid and among plants of each version, (ii) to observe the distribution of genetic diversity along the genome and (iii) to explore relationships between phenotypic variability and genetic diversity. Hybrids were cultivated at field conditions during two growing seasons and plants of each hybrid with high, intermediate and low biomass at physiological maturity were selected to perform a study of single nucleotide polymorphisms (SNP). Genetic diversity among plants of each version was greater than among versions and both sources of variation were significant (ΦST = 0.45, P < 0.01). Genetic diversity of the non-transgenic DK747 was higher than those of the transgenic versions, probably reflecting the conventional breeding history of these iso-hybrids. Similarity coefficients indicate that the most homogeneous group was that composed by plants of DK747MGRR. A Fisher's exact test together with a principal component analysis identified certain SNPs related to the contrasting plant biomass of DK747, 747MG and DK747RR. Caution should be taken with these results, because of the small sample size for SNPs study and the narrow set of tested hybrids.Fil: Laserna, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: López, C. G.. Universidad Nacional de Lomas de Zamora; ArgentinaFil: Aulicino, Mónica Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Instituto Fitotécnico de "Santa Catalina"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maddonni, Gustavo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaElsevier Science2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17447Laserna, María Paula; López, C. G.; Aulicino, Mónica Beatriz; Maddonni, Gustavo Angel; Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid; Elsevier Science; Field Crops Research; 176; 5-2015; 56-600378-4290enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fcr.2015.02.012info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378429015000544info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:15:30Zoai:ri.conicet.gov.ar:11336/17447instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:15:30.846CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
title Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
spellingShingle Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
Laserna, María Paula
Zea Mays
Single Nucleotide Polymorphism
Genetic Diversity
Non-Transgenic Hybrid
Transgenic Hybridation
title_short Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
title_full Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
title_fullStr Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
title_full_unstemmed Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
title_sort Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid
dc.creator.none.fl_str_mv Laserna, María Paula
López, C. G.
Aulicino, Mónica Beatriz
Maddonni, Gustavo Angel
author Laserna, María Paula
author_facet Laserna, María Paula
López, C. G.
Aulicino, Mónica Beatriz
Maddonni, Gustavo Angel
author_role author
author2 López, C. G.
Aulicino, Mónica Beatriz
Maddonni, Gustavo Angel
author2_role author
author
author
dc.subject.none.fl_str_mv Zea Mays
Single Nucleotide Polymorphism
Genetic Diversity
Non-Transgenic Hybrid
Transgenic Hybridation
topic Zea Mays
Single Nucleotide Polymorphism
Genetic Diversity
Non-Transgenic Hybrid
Transgenic Hybridation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Previous studies have documented that transgene introduction may alter the phenotypic expression of several traits (e.g., biomass production, grain yield). We hypothesized that genetic diversity could influence the phenotypic variation among hybrids of a same genetic background and also among plants of a hybrid. The objectives of this preliminary study were: (i) to quantify the genetic diversity between the non-transgenic (DK747) and transgenic versions (DK747MG, DK747RR and DK747MGRR) of a single-cross maize hybrid and among plants of each version, (ii) to observe the distribution of genetic diversity along the genome and (iii) to explore relationships between phenotypic variability and genetic diversity. Hybrids were cultivated at field conditions during two growing seasons and plants of each hybrid with high, intermediate and low biomass at physiological maturity were selected to perform a study of single nucleotide polymorphisms (SNP). Genetic diversity among plants of each version was greater than among versions and both sources of variation were significant (ΦST = 0.45, P < 0.01). Genetic diversity of the non-transgenic DK747 was higher than those of the transgenic versions, probably reflecting the conventional breeding history of these iso-hybrids. Similarity coefficients indicate that the most homogeneous group was that composed by plants of DK747MGRR. A Fisher's exact test together with a principal component analysis identified certain SNPs related to the contrasting plant biomass of DK747, 747MG and DK747RR. Caution should be taken with these results, because of the small sample size for SNPs study and the narrow set of tested hybrids.
Fil: Laserna, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: López, C. G.. Universidad Nacional de Lomas de Zamora; Argentina
Fil: Aulicino, Mónica Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Instituto Fitotécnico de "Santa Catalina"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maddonni, Gustavo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
description Previous studies have documented that transgene introduction may alter the phenotypic expression of several traits (e.g., biomass production, grain yield). We hypothesized that genetic diversity could influence the phenotypic variation among hybrids of a same genetic background and also among plants of a hybrid. The objectives of this preliminary study were: (i) to quantify the genetic diversity between the non-transgenic (DK747) and transgenic versions (DK747MG, DK747RR and DK747MGRR) of a single-cross maize hybrid and among plants of each version, (ii) to observe the distribution of genetic diversity along the genome and (iii) to explore relationships between phenotypic variability and genetic diversity. Hybrids were cultivated at field conditions during two growing seasons and plants of each hybrid with high, intermediate and low biomass at physiological maturity were selected to perform a study of single nucleotide polymorphisms (SNP). Genetic diversity among plants of each version was greater than among versions and both sources of variation were significant (ΦST = 0.45, P < 0.01). Genetic diversity of the non-transgenic DK747 was higher than those of the transgenic versions, probably reflecting the conventional breeding history of these iso-hybrids. Similarity coefficients indicate that the most homogeneous group was that composed by plants of DK747MGRR. A Fisher's exact test together with a principal component analysis identified certain SNPs related to the contrasting plant biomass of DK747, 747MG and DK747RR. Caution should be taken with these results, because of the small sample size for SNPs study and the narrow set of tested hybrids.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17447
Laserna, María Paula; López, C. G.; Aulicino, Mónica Beatriz; Maddonni, Gustavo Angel; Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid; Elsevier Science; Field Crops Research; 176; 5-2015; 56-60
0378-4290
url http://hdl.handle.net/11336/17447
identifier_str_mv Laserna, María Paula; López, C. G.; Aulicino, Mónica Beatriz; Maddonni, Gustavo Angel; Genetic diversity among plants of non-transgenic and transgenic versions of a single cross maize hybrid; Elsevier Science; Field Crops Research; 176; 5-2015; 56-60
0378-4290
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fcr.2015.02.012
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378429015000544
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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