Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-...

Autores: Panei, Carlos Javier; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; Ishizaki, Hiroshi; Matoba, Kazuhiro; Aida, Yoko
Año de publicación: 2013
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most commonneoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistentlymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+Tcells,CD3+Tcells,CD4+Tcells,CD8+Tcells,γ/δT cells, monocytes, and granulocytes in infected cattle that do not have tumors,although the most consistently infected cell is the CD5+B cell. The mechanism by which BLV causes uncontrolledCD5+B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chainreaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates notonly with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The presentstudy reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated fromBLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCR
Fil: Panei, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Discovery Riken Institute; Japón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Takeshima, Shin-nosuke. Discovery Riken Institute; Japón
Fil: Omori, Takashi. Nippon Institute For Biological Sciences; Japón
Fil: Nunoya, Tetsuo. Nippon Institute For Biological Sciences; Japón
Fil: Davis, William C.. Washington State University; Estados Unidos
Fil: Ishizaki, Hiroshi. Naro Institute Of Livestock And Grassland Sciences; Japón
Fil: Matoba, Kazuhiro. Naro Institute Of Livestock And Grassland Sciences; Japón
Fil: Aida, Yoko. Discovery Riken Institute; Japón
Materia: Bovine leukemia virus (BLV)
proviral load
BLV-CoCoMo-qPCR
CD5+IgM+ cell
flow cytometry
cell sorting
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/41613

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCRPanei, Carlos JavierTakeshima, Shin-nosukeOmori, TakashiNunoya, TetsuoDavis, William C.Ishizaki, HiroshiMatoba, KazuhiroAida, YokoBovine leukemia virus (BLV)proviral loadBLV-CoCoMo-qPCRCD5+IgM+ cellflow cytometrycell sortinghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most commonneoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistentlymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+Tcells,CD3+Tcells,CD4+Tcells,CD8+Tcells,γ/δT cells, monocytes, and granulocytes in infected cattle that do not have tumors,although the most consistently infected cell is the CD5+B cell. The mechanism by which BLV causes uncontrolledCD5+B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chainreaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates notonly with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The presentstudy reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated fromBLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCRFil: Panei, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Discovery Riken Institute; Japón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Takeshima, Shin-nosuke. Discovery Riken Institute; JapónFil: Omori, Takashi. Nippon Institute For Biological Sciences; JapónFil: Nunoya, Tetsuo. Nippon Institute For Biological Sciences; JapónFil: Davis, William C.. Washington State University; Estados UnidosFil: Ishizaki, Hiroshi. Naro Institute Of Livestock And Grassland Sciences; JapónFil: Matoba, Kazuhiro. Naro Institute Of Livestock And Grassland Sciences; JapónFil: Aida, Yoko. Discovery Riken Institute; JapónBioMed Central2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41613Panei, Carlos Javier; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; et al.; Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR; BioMed Central; BMC Veterinary Research; 9; 95; 3-2013; 1-81746-6148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-9-95info:eu-repo/semantics/altIdentifier/doi/10.1186/1746-6148-9-95info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:51Zoai:ri.conicet.gov.ar:11336/41613instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:51.906CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
title	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
spellingShingle	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR Panei, Carlos Javier Bovine leukemia virus (BLV) proviral load BLV-CoCoMo-qPCR CD5+IgM+ cell flow cytometry cell sorting
title_short	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
title_full	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
title_fullStr	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
title_full_unstemmed	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
title_sort	Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
dc.creator.none.fl_str_mv	Panei, Carlos Javier Takeshima, Shin-nosuke Omori, Takashi Nunoya, Tetsuo Davis, William C. Ishizaki, Hiroshi Matoba, Kazuhiro Aida, Yoko
author	Panei, Carlos Javier
author_facet	Panei, Carlos Javier Takeshima, Shin-nosuke Omori, Takashi Nunoya, Tetsuo Davis, William C. Ishizaki, Hiroshi Matoba, Kazuhiro Aida, Yoko
author_role	author
author2	Takeshima, Shin-nosuke Omori, Takashi Nunoya, Tetsuo Davis, William C. Ishizaki, Hiroshi Matoba, Kazuhiro Aida, Yoko
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Bovine leukemia virus (BLV) proviral load BLV-CoCoMo-qPCR CD5+IgM+ cell flow cytometry cell sorting
topic	Bovine leukemia virus (BLV) proviral load BLV-CoCoMo-qPCR CD5+IgM+ cell flow cytometry cell sorting
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/4.2 https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv	Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most commonneoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistentlymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+Tcells,CD3+Tcells,CD4+Tcells,CD8+Tcells,γ/δT cells, monocytes, and granulocytes in infected cattle that do not have tumors,although the most consistently infected cell is the CD5+B cell. The mechanism by which BLV causes uncontrolledCD5+B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chainreaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates notonly with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The presentstudy reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated fromBLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCR Fil: Panei, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Discovery Riken Institute; Japón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina Fil: Takeshima, Shin-nosuke. Discovery Riken Institute; Japón Fil: Omori, Takashi. Nippon Institute For Biological Sciences; Japón Fil: Nunoya, Tetsuo. Nippon Institute For Biological Sciences; Japón Fil: Davis, William C.. Washington State University; Estados Unidos Fil: Ishizaki, Hiroshi. Naro Institute Of Livestock And Grassland Sciences; Japón Fil: Matoba, Kazuhiro. Naro Institute Of Livestock And Grassland Sciences; Japón Fil: Aida, Yoko. Discovery Riken Institute; Japón
description	Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most commonneoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistentlymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+Tcells,CD3+Tcells,CD4+Tcells,CD8+Tcells,γ/δT cells, monocytes, and granulocytes in infected cattle that do not have tumors,although the most consistently infected cell is the CD5+B cell. The mechanism by which BLV causes uncontrolledCD5+B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chainreaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates notonly with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The presentstudy reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated fromBLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCR
publishDate	2013
dc.date.none.fl_str_mv	2013-03
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/41613 Panei, Carlos Javier; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; et al.; Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR; BioMed Central; BMC Veterinary Research; 9; 95; 3-2013; 1-8 1746-6148 CONICET Digital CONICET
url	http://hdl.handle.net/11336/41613
identifier_str_mv	Panei, Carlos Javier; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; et al.; Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR; BioMed Central; BMC Veterinary Research; 9; 95; 3-2013; 1-8 1746-6148 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-9-95 info:eu-repo/semantics/altIdentifier/doi/10.1186/1746-6148-9-95
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	BioMed Central
publisher.none.fl_str_mv	BioMed Central
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-...

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