α7 Nicotinic expression and function in human retinal pigment epitheliu cells
- Autores
- Mader, Julieta Ailén; Fernandez Delias, María Florencia; Chrestia, Juan Facundo; Sotelo, Florencia Anahí; Esandi, Maria del Carmen; Bouzat, Cecilia Beatriz
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The α7 nicotinic receptor (nAChR) is one of the most abundant nAChRs in the nervous system and is also present in non-neuronal cells, including immune and epithelial cells. It is involved in cognition, memory, pain, neuroprotection and inflammation and its potentiation has emerged as a therapeutic strategy for neurological, neurodegenerative, and inflammatory disorders. Given that the increase in oxidative stress in retinal pigment epithelial cells contributes to the development of age-related macular degeneration and that α7 activation exerts cell protective effects, we explored the presence and functional relevance of α7 in D407 retinal pigment epithelium cells. By confocal microscopy using the α7 specific antagonist α-bungarotoxin labeled with Alexa 488 and real time-PCR we demonstrated the presence of α7 in these epithelial cells. To simulate the events occurring in age-related macular degeneration, we treated cells with 500 µM ferric ammonium citrate (FAC) for 48 h to induce stress damage and measured reactive oxygen species (ROS) with the fluorescent probe DCFH-DA and cell viability by the MTT assay. FAC treatment resulted in a significant 56 ± 23% increase in ROS levels with respect to the control. To determine if α7 protects against oxidative damage, we exposed cells for 4 h to a specific α7 agonist, PNU-282987, before the FAC treatment. This exposure reduced 29 ± 3% basal levels of ROS. Notably, PNU-282987 exhibited protective effects against the FAC treatment, leading to a reduction of 46 ± 15% in ROS levels when compared to the treated cells. In line with these observations, exposure to the α7 agonist restored the 20% reduction in cell viability induced by FAC. Overall, we demonstrated for the first time the presence of α7 in the D407 cell line, which is a model system for studying various retinal diseases, and its protective role against oxidative damage, a key factor linked to the onset of macular degeneration
Fil: Mader, Julieta Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Sotelo, Florencia Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Esandi, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Reunión Conjunta SAIC SAB AAFE AACYTAL 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (saic); XXV Annual Conferences of Sociedad Argentina de Biología (sab); IV Annual Meeting of Asociación Argentina de Farmacología Experimental (aafe); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (aacytal)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio - Materia
-
ALFA 7
Nicotinic receptor
ROS
D407 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/221663
Ver los metadatos del registro completo
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α7 Nicotinic expression and function in human retinal pigment epitheliu cellsMader, Julieta AilénFernandez Delias, María FlorenciaChrestia, Juan FacundoSotelo, Florencia AnahíEsandi, Maria del CarmenBouzat, Cecilia BeatrizALFA 7Nicotinic receptorROSD407https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The α7 nicotinic receptor (nAChR) is one of the most abundant nAChRs in the nervous system and is also present in non-neuronal cells, including immune and epithelial cells. It is involved in cognition, memory, pain, neuroprotection and inflammation and its potentiation has emerged as a therapeutic strategy for neurological, neurodegenerative, and inflammatory disorders. Given that the increase in oxidative stress in retinal pigment epithelial cells contributes to the development of age-related macular degeneration and that α7 activation exerts cell protective effects, we explored the presence and functional relevance of α7 in D407 retinal pigment epithelium cells. By confocal microscopy using the α7 specific antagonist α-bungarotoxin labeled with Alexa 488 and real time-PCR we demonstrated the presence of α7 in these epithelial cells. To simulate the events occurring in age-related macular degeneration, we treated cells with 500 µM ferric ammonium citrate (FAC) for 48 h to induce stress damage and measured reactive oxygen species (ROS) with the fluorescent probe DCFH-DA and cell viability by the MTT assay. FAC treatment resulted in a significant 56 ± 23% increase in ROS levels with respect to the control. To determine if α7 protects against oxidative damage, we exposed cells for 4 h to a specific α7 agonist, PNU-282987, before the FAC treatment. This exposure reduced 29 ± 3% basal levels of ROS. Notably, PNU-282987 exhibited protective effects against the FAC treatment, leading to a reduction of 46 ± 15% in ROS levels when compared to the treated cells. In line with these observations, exposure to the α7 agonist restored the 20% reduction in cell viability induced by FAC. Overall, we demonstrated for the first time the presence of α7 in the D407 cell line, which is a model system for studying various retinal diseases, and its protective role against oxidative damage, a key factor linked to the onset of macular degenerationFil: Mader, Julieta Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Sotelo, Florencia Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Esandi, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaReunión Conjunta SAIC SAB AAFE AACYTAL 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (saic); XXV Annual Conferences of Sociedad Argentina de Biología (sab); IV Annual Meeting of Asociación Argentina de Farmacología Experimental (aafe); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (aacytal)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicinade Vito, EduardoLüthy, IsabelLaudanno, Oscar M.Narvaiz Kantor, Isabel2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221663α7 Nicotinic expression and function in human retinal pigment epitheliu cells; Reunión Conjunta SAIC SAB AAFE AACYTAL 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (saic); XXV Annual Conferences of Sociedad Argentina de Biología (sab); IV Annual Meeting of Asociación Argentina de Farmacología Experimental (aafe); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (aacytal); Mar del Plata; Argentina; 2023; 224-2251669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:25Zoai:ri.conicet.gov.ar:11336/221663instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:26.259CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells |
| title |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells |
| spellingShingle |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells Mader, Julieta Ailén ALFA 7 Nicotinic receptor ROS D407 |
| title_short |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells |
| title_full |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells |
| title_fullStr |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells |
| title_full_unstemmed |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells |
| title_sort |
α7 Nicotinic expression and function in human retinal pigment epitheliu cells |
| dc.creator.none.fl_str_mv |
Mader, Julieta Ailén Fernandez Delias, María Florencia Chrestia, Juan Facundo Sotelo, Florencia Anahí Esandi, Maria del Carmen Bouzat, Cecilia Beatriz |
| author |
Mader, Julieta Ailén |
| author_facet |
Mader, Julieta Ailén Fernandez Delias, María Florencia Chrestia, Juan Facundo Sotelo, Florencia Anahí Esandi, Maria del Carmen Bouzat, Cecilia Beatriz |
| author_role |
author |
| author2 |
Fernandez Delias, María Florencia Chrestia, Juan Facundo Sotelo, Florencia Anahí Esandi, Maria del Carmen Bouzat, Cecilia Beatriz |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
de Vito, Eduardo Lüthy, Isabel Laudanno, Oscar M. Narvaiz Kantor, Isabel |
| dc.subject.none.fl_str_mv |
ALFA 7 Nicotinic receptor ROS D407 |
| topic |
ALFA 7 Nicotinic receptor ROS D407 |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
The α7 nicotinic receptor (nAChR) is one of the most abundant nAChRs in the nervous system and is also present in non-neuronal cells, including immune and epithelial cells. It is involved in cognition, memory, pain, neuroprotection and inflammation and its potentiation has emerged as a therapeutic strategy for neurological, neurodegenerative, and inflammatory disorders. Given that the increase in oxidative stress in retinal pigment epithelial cells contributes to the development of age-related macular degeneration and that α7 activation exerts cell protective effects, we explored the presence and functional relevance of α7 in D407 retinal pigment epithelium cells. By confocal microscopy using the α7 specific antagonist α-bungarotoxin labeled with Alexa 488 and real time-PCR we demonstrated the presence of α7 in these epithelial cells. To simulate the events occurring in age-related macular degeneration, we treated cells with 500 µM ferric ammonium citrate (FAC) for 48 h to induce stress damage and measured reactive oxygen species (ROS) with the fluorescent probe DCFH-DA and cell viability by the MTT assay. FAC treatment resulted in a significant 56 ± 23% increase in ROS levels with respect to the control. To determine if α7 protects against oxidative damage, we exposed cells for 4 h to a specific α7 agonist, PNU-282987, before the FAC treatment. This exposure reduced 29 ± 3% basal levels of ROS. Notably, PNU-282987 exhibited protective effects against the FAC treatment, leading to a reduction of 46 ± 15% in ROS levels when compared to the treated cells. In line with these observations, exposure to the α7 agonist restored the 20% reduction in cell viability induced by FAC. Overall, we demonstrated for the first time the presence of α7 in the D407 cell line, which is a model system for studying various retinal diseases, and its protective role against oxidative damage, a key factor linked to the onset of macular degeneration Fil: Mader, Julieta Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Sotelo, Florencia Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Esandi, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Reunión Conjunta SAIC SAB AAFE AACYTAL 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (saic); XXV Annual Conferences of Sociedad Argentina de Biología (sab); IV Annual Meeting of Asociación Argentina de Farmacología Experimental (aafe); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (aacytal) Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Biología Asociación Argentina de Farmacología Experimental Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio |
| description |
The α7 nicotinic receptor (nAChR) is one of the most abundant nAChRs in the nervous system and is also present in non-neuronal cells, including immune and epithelial cells. It is involved in cognition, memory, pain, neuroprotection and inflammation and its potentiation has emerged as a therapeutic strategy for neurological, neurodegenerative, and inflammatory disorders. Given that the increase in oxidative stress in retinal pigment epithelial cells contributes to the development of age-related macular degeneration and that α7 activation exerts cell protective effects, we explored the presence and functional relevance of α7 in D407 retinal pigment epithelium cells. By confocal microscopy using the α7 specific antagonist α-bungarotoxin labeled with Alexa 488 and real time-PCR we demonstrated the presence of α7 in these epithelial cells. To simulate the events occurring in age-related macular degeneration, we treated cells with 500 µM ferric ammonium citrate (FAC) for 48 h to induce stress damage and measured reactive oxygen species (ROS) with the fluorescent probe DCFH-DA and cell viability by the MTT assay. FAC treatment resulted in a significant 56 ± 23% increase in ROS levels with respect to the control. To determine if α7 protects against oxidative damage, we exposed cells for 4 h to a specific α7 agonist, PNU-282987, before the FAC treatment. This exposure reduced 29 ± 3% basal levels of ROS. Notably, PNU-282987 exhibited protective effects against the FAC treatment, leading to a reduction of 46 ± 15% in ROS levels when compared to the treated cells. In line with these observations, exposure to the α7 agonist restored the 20% reduction in cell viability induced by FAC. Overall, we demonstrated for the first time the presence of α7 in the D407 cell line, which is a model system for studying various retinal diseases, and its protective role against oxidative damage, a key factor linked to the onset of macular degeneration |
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2023 |
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2023 |
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http://hdl.handle.net/11336/221663 α7 Nicotinic expression and function in human retinal pigment epitheliu cells; Reunión Conjunta SAIC SAB AAFE AACYTAL 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (saic); XXV Annual Conferences of Sociedad Argentina de Biología (sab); IV Annual Meeting of Asociación Argentina de Farmacología Experimental (aafe); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (aacytal); Mar del Plata; Argentina; 2023; 224-225 1669-9106 CONICET Digital CONICET |
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α7 Nicotinic expression and function in human retinal pigment epitheliu cells; Reunión Conjunta SAIC SAB AAFE AACYTAL 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (saic); XXV Annual Conferences of Sociedad Argentina de Biología (sab); IV Annual Meeting of Asociación Argentina de Farmacología Experimental (aafe); VIII Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (aacytal); Mar del Plata; Argentina; 2023; 224-225 1669-9106 CONICET Digital CONICET |
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eng |
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