Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations

• Autores: Gavara, Poondi Rajesh; Cabrera, Rosa; Vennapusa, Rami Reddy; Grasselli, Mariano; Fernandez-Lahore, Marcelo
• Año de publicación: 2012
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Column Packing; Composite Fibrous Adsorbent; Dynamic Binding Capacity; Hydrogel; Permeability Coefficient; Resolution Factor
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Fibrous materials are proposed as novel chromatographic supports depicting high throughput and high product capacity. In this work, a composite fiber harboring strong cation-exchange moieties has been investigated. Such materials were characterized by a plethora of physical methods including degree of swelling (DS), scanning electron microscope (SEM), confocal laser scanning microscopy (CLSM), and Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR). The composite showed a high degree of grafting (∼30%) and exhibited a high swelling ratio (∼300%). Moreover, homogenous grafting and the development of an internal (functional) hydrogel were observed. The fibrous adsorbent was packed utilizing a designed " double roll" supported-structure and subsequently tested for packing efficiency and chromatography performance. The mentioned system showed similar packing efficiency of height equivalent to a theoretical plate (HETP) value and higher permeability coefficient (0.92×10 -7cm 2) than commercial resins. Experimentally determined Peclet number (Pe) values were within the range 60-90, suggesting a close-to-plug-flow condition. Total ionic capacity of the fibrous adsorbent was determined by the transition pH method. A capacity of 6.5mequiv./g was obtained. Moreover, a high dynamic binding capacity for lysozyme was found to be 283mg/g. On the other hand, a bed of randomly packed fiber also demonstrated high-resolution ability when a mixture of model protein was utilized to that end. Resolution was maintained at high flow rates (up to 900cm/h) and utilizing shorter gradient development routines. Direct sequestration of a model protein (lysozyme) was also possible from an artificial mixture containing 1.5% yeast homogenate. Summarizing, the composite fibrous adsorbents exhibited superior performance during early protein capture and intermediate-resolution applications.
Fil: Gavara, Poondi Rajesh. Universitat Bremen; Alemania
Fil: Cabrera, Rosa. Universitat Bremen; Alemania
Fil: Vennapusa, Rami Reddy. Universitat Bremen; Alemania
Fil: Grasselli, Mariano. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Fernandez-Lahore, Marcelo. Universitat Bremen; Alemania