Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry

Autores
Lagares, Antonio; Valverde, Claudio Fabián
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Materia
Polyhydroxybutyrate
Phb
Bacteria
Nile Red
Flow Cytometry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/74268
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/74268
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantification of Bacterial Polyhydroxybutyrate Content by Flow CytometryLagares, AntonioValverde, Claudio FabiánPolyhydroxybutyratePhbBacteriaNile RedFlow Cytometryhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaBio-Protocol LLC2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/74268Lagares, Antonio; Valverde, Claudio Fabián; Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry; Bio-Protocol LLC; Bio-Protocol; 7; 23; 12-2017; 1-72331-8325CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.2638info:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e2638info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:22Zoai:ri.conicet.gov.ar:11336/74268instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:22.698CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
title Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
spellingShingle Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
Lagares, Antonio
Polyhydroxybutyrate
Phb
Bacteria
Nile Red
Flow Cytometry
title_short Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
title_full Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
title_fullStr Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
title_full_unstemmed Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
title_sort Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
dc.creator.none.fl_str_mv Lagares, Antonio
Valverde, Claudio Fabián
author Lagares, Antonio
author_facet Lagares, Antonio
Valverde, Claudio Fabián
author_role author
author2 Valverde, Claudio Fabián
author2_role author
dc.subject.none.fl_str_mv Polyhydroxybutyrate
Phb
Bacteria
Nile Red
Flow Cytometry
topic Polyhydroxybutyrate
Phb
Bacteria
Nile Red
Flow Cytometry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
description We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/74268
Lagares, Antonio; Valverde, Claudio Fabián; Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry; Bio-Protocol LLC; Bio-Protocol; 7; 23; 12-2017; 1-7
2331-8325
CONICET Digital
CONICET
url http://hdl.handle.net/11336/74268
identifier_str_mv Lagares, Antonio; Valverde, Claudio Fabián; Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry; Bio-Protocol LLC; Bio-Protocol; 7; 23; 12-2017; 1-7
2331-8325
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.2638
info:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e2638
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-Protocol LLC
publisher.none.fl_str_mv Bio-Protocol LLC
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268597838151680
score 13.13397

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