Sperm membrane functionality in the dog assessed by flow cytometry
- Autores
- Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; Sánchez, Raúl; Risopatrón, Jennie
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa.
Fil: Cheuquemán, Carolina. Universidad de La Frontera; Chile
Fil: Bravo, P.. Universidad de La Frontera; Chile
Fil: Treulén, Favian. Universidad de La Frontera; Chile
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Villegas, Juana. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Sánchez, Raúl. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina - Materia
-
Spermatozoa
Dog
Flow Cytometry - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/42645
Ver los metadatos del registro completo
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Sperm membrane functionality in the dog assessed by flow cytometryCheuquemán, CarolinaBravo, P.Treulén, FavianGiojalas, Laura CeciliaVillegas, JuanaSánchez, RaúlRisopatrón, JennieSpermatozoaDogFlow Cytometryhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa.Fil: Cheuquemán, Carolina. Universidad de La Frontera; ChileFil: Bravo, P.. Universidad de La Frontera; ChileFil: Treulén, Favian. Universidad de La Frontera; ChileFil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Villegas, Juana. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Sánchez, Raúl. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Risopatrón, Jennie. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaWiley Blackwell Publishing, Inc2012-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42645Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; et al.; Sperm membrane functionality in the dog assessed by flow cytometry; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 1; 28-2-2012; 39-430936-67681439-0531CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2011.01798.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2011.01798.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:26Zoai:ri.conicet.gov.ar:11336/42645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:26.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sperm membrane functionality in the dog assessed by flow cytometry |
title |
Sperm membrane functionality in the dog assessed by flow cytometry |
spellingShingle |
Sperm membrane functionality in the dog assessed by flow cytometry Cheuquemán, Carolina Spermatozoa Dog Flow Cytometry |
title_short |
Sperm membrane functionality in the dog assessed by flow cytometry |
title_full |
Sperm membrane functionality in the dog assessed by flow cytometry |
title_fullStr |
Sperm membrane functionality in the dog assessed by flow cytometry |
title_full_unstemmed |
Sperm membrane functionality in the dog assessed by flow cytometry |
title_sort |
Sperm membrane functionality in the dog assessed by flow cytometry |
dc.creator.none.fl_str_mv |
Cheuquemán, Carolina Bravo, P. Treulén, Favian Giojalas, Laura Cecilia Villegas, Juana Sánchez, Raúl Risopatrón, Jennie |
author |
Cheuquemán, Carolina |
author_facet |
Cheuquemán, Carolina Bravo, P. Treulén, Favian Giojalas, Laura Cecilia Villegas, Juana Sánchez, Raúl Risopatrón, Jennie |
author_role |
author |
author2 |
Bravo, P. Treulén, Favian Giojalas, Laura Cecilia Villegas, Juana Sánchez, Raúl Risopatrón, Jennie |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Spermatozoa Dog Flow Cytometry |
topic |
Spermatozoa Dog Flow Cytometry |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa. Fil: Cheuquemán, Carolina. Universidad de La Frontera; Chile Fil: Bravo, P.. Universidad de La Frontera; Chile Fil: Treulén, Favian. Universidad de La Frontera; Chile Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina Fil: Villegas, Juana. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina Fil: Sánchez, Raúl. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina |
description |
The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-28 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/42645 Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; et al.; Sperm membrane functionality in the dog assessed by flow cytometry; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 1; 28-2-2012; 39-43 0936-6768 1439-0531 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/42645 |
identifier_str_mv |
Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; et al.; Sperm membrane functionality in the dog assessed by flow cytometry; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 1; 28-2-2012; 39-43 0936-6768 1439-0531 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2011.01798.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2011.01798.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |