Sperm membrane functionality in the dog assessed by flow cytometry

Autores
Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; Sánchez, Raúl; Risopatrón, Jennie
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa.
Fil: Cheuquemán, Carolina. Universidad de La Frontera; Chile
Fil: Bravo, P.. Universidad de La Frontera; Chile
Fil: Treulén, Favian. Universidad de La Frontera; Chile
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Villegas, Juana. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Sánchez, Raúl. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Materia
Spermatozoa
Dog
Flow Cytometry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42645
Acceder
id CONICETDig_2e77b911132a33ec02833e76a902b6a4
oai_identifier_str oai:ri.conicet.gov.ar:11336/42645
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sperm membrane functionality in the dog assessed by flow cytometryCheuquemán, CarolinaBravo, P.Treulén, FavianGiojalas, Laura CeciliaVillegas, JuanaSánchez, RaúlRisopatrón, JennieSpermatozoaDogFlow Cytometryhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa.Fil: Cheuquemán, Carolina. Universidad de La Frontera; ChileFil: Bravo, P.. Universidad de La Frontera; ChileFil: Treulén, Favian. Universidad de La Frontera; ChileFil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Villegas, Juana. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Sánchez, Raúl. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Risopatrón, Jennie. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaWiley Blackwell Publishing, Inc2012-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42645Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; et al.; Sperm membrane functionality in the dog assessed by flow cytometry; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 1; 28-2-2012; 39-430936-67681439-0531CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2011.01798.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2011.01798.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:53Zoai:ri.conicet.gov.ar:11336/42645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:53.877CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sperm membrane functionality in the dog assessed by flow cytometry
title Sperm membrane functionality in the dog assessed by flow cytometry
spellingShingle Sperm membrane functionality in the dog assessed by flow cytometry
Cheuquemán, Carolina
Spermatozoa
Dog
Flow Cytometry
title_short Sperm membrane functionality in the dog assessed by flow cytometry
title_full Sperm membrane functionality in the dog assessed by flow cytometry
title_fullStr Sperm membrane functionality in the dog assessed by flow cytometry
title_full_unstemmed Sperm membrane functionality in the dog assessed by flow cytometry
title_sort Sperm membrane functionality in the dog assessed by flow cytometry
dc.creator.none.fl_str_mv Cheuquemán, Carolina
Bravo, P.
Treulén, Favian
Giojalas, Laura Cecilia
Villegas, Juana
Sánchez, Raúl
Risopatrón, Jennie
author Cheuquemán, Carolina
author_facet Cheuquemán, Carolina
Bravo, P.
Treulén, Favian
Giojalas, Laura Cecilia
Villegas, Juana
Sánchez, Raúl
Risopatrón, Jennie
author_role author
author2 Bravo, P.
Treulén, Favian
Giojalas, Laura Cecilia
Villegas, Juana
Sánchez, Raúl
Risopatrón, Jennie
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Spermatozoa
Dog
Flow Cytometry
topic Spermatozoa
Dog
Flow Cytometry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa.
Fil: Cheuquemán, Carolina. Universidad de La Frontera; Chile
Fil: Bravo, P.. Universidad de La Frontera; Chile
Fil: Treulén, Favian. Universidad de La Frontera; Chile
Fil: Giojalas, Laura Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Villegas, Juana. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Sánchez, Raúl. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Risopatrón, Jennie. Universidad de La Frontera; Chile. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
description The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa. The second fractions of ejaculates from six dogs were pooled, and samples (n = 26) processed to determine the variables: sperm viability and plasma membrane integrity staining; phosphatidylserine (PS) translocation by Annexin-V-FITC and PI labelling; acrosome membrane integrity by FITC-conjugated Pisum sativum agglutinin ⁄ PI labelling; and mitochondrial membrane potential (DYm) by staining with. Means for the 26 examined samples indicated that 82.66 ± 2.8% of the viable spermatozoa showed an intact plasma membrane, 8.4 ± 2.6% were moribund, 72.7 ± 16% had an intact acrosome, 80.9 ± 17% had high DYm and 8.1 ± 11% had PS translocation with a PS translocation index of 2.1 ± 3%. Motility was only correlated with PS translo-cation (R = 0.3901; p = 0.0488), and acrosome membrane integrity was correlated with PS translocation (R = 0.5816;>p = 0.0018). This study provides objective physiological data on the functional capacity of canine spermatozoa.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42645
Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; et al.; Sperm membrane functionality in the dog assessed by flow cytometry; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 1; 28-2-2012; 39-43
0936-6768
1439-0531
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42645
identifier_str_mv Cheuquemán, Carolina; Bravo, P.; Treulén, Favian; Giojalas, Laura Cecilia; Villegas, Juana; et al.; Sperm membrane functionality in the dog assessed by flow cytometry; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 47; 1; 28-2-2012; 39-43
0936-6768
1439-0531
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2011.01798.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2011.01798.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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