Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)

Autores
Pacheco, Mariana Soledad; Dominguez, Facundo Gabriel; Torres, Mariela Analía; Martorell, María Martha; Ruberto, Lucas Adolfo Mauro; Mac Cormack, Walter Patricio; Martinez Alvarez, Lucas Manuel; Pajot, Hipolito Fernando; Nieto Peñalver, Carlos Gabriel
Año de publicación
2023
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Antarctic environments typically present low temperatures, high solar radiation and low nutrient availability, being one of the harshestenvironments on Earth. Psychrophilic and psychrotolerant yeasts from Antarctic soils and cryoconites have adapted to such conditions,being an interesting source of new enzymes with great biotechnological potential. This work intends to prove the textile dye decolorizingpotential of 139 yeasts isolated from soil and cryoconite samples from 25 de Mayo / King George Island, Antarctica. Isolates were cultivatedin YM, and NDM (Normal Decolorization Medium) media at 15, 20 and 25°C, and classified into psychrophilic and psychrotolerantaccording to their growth profiles. Textile dye decolorization was evaluated in the same two media, plus 200 mg/L of one of fourcommercially available reactive azo dyes: Vilmafix® Blue RR-BB (CI, Reactive Blue 221), Vilmafix® Red 7B-HE (CI, Reactive Red141), Vilmafix® Black B-V (CI, Reactive Black 5), or Vilmafix® Green RR-4B (CI, Reactive Green). Plates were incubated for 72 h at15, 20 or 25°C, depending on the yeast isolate. Dye decolorization haloes and colony dyeing were recorded daily and used to select themost promising isolates. Of 139 isolates, 34% were classified as psychrophilic (growing only at 15°C) while the remaining 66% wereclassified as psychrotolerant (growing at all the assayed temperatures), irrespective of the medium assayed. Forty-five isolates producehaloes in at least one of the tested dyes in NDM, while only 40 yeast produced haloes in YM. 15 isolates were selected for further studiesIsolates A075 and Y28D produced intense haloes without colony dyeing; isolates Y67, Y70 and Y75 produced intense haloes and get dyedonly at the edge of the colonies while the remaining ten isolates, A092, A099, A104, A105, A106, A107, Y6, Y59, Y73 and Y84, producedneat haloes with a significant colorization of the entire colonies. The obtained results prove the biotechnological potential of Antarcticyeasts, raising the possibility of designing more efficient dye-decolorizing methods at low temperatures.
Fil: Pacheco, Mariana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Dominguez, Facundo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Martinez Alvarez, Lucas Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XL Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Materia
DECOLORATION
TEXTILE DYE
YEAST
ANTARCTICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213140
Acceder
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spelling Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)Pacheco, Mariana SoledadDominguez, Facundo GabrielTorres, Mariela AnalíaMartorell, María MarthaRuberto, Lucas Adolfo MauroMac Cormack, Walter PatricioMartinez Alvarez, Lucas ManuelPajot, Hipolito FernandoNieto Peñalver, Carlos GabrielDECOLORATIONTEXTILE DYEYEASTANTARCTICAhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Antarctic environments typically present low temperatures, high solar radiation and low nutrient availability, being one of the harshestenvironments on Earth. Psychrophilic and psychrotolerant yeasts from Antarctic soils and cryoconites have adapted to such conditions,being an interesting source of new enzymes with great biotechnological potential. This work intends to prove the textile dye decolorizingpotential of 139 yeasts isolated from soil and cryoconite samples from 25 de Mayo / King George Island, Antarctica. Isolates were cultivatedin YM, and NDM (Normal Decolorization Medium) media at 15, 20 and 25°C, and classified into psychrophilic and psychrotolerantaccording to their growth profiles. Textile dye decolorization was evaluated in the same two media, plus 200 mg/L of one of fourcommercially available reactive azo dyes: Vilmafix® Blue RR-BB (CI, Reactive Blue 221), Vilmafix® Red 7B-HE (CI, Reactive Red141), Vilmafix® Black B-V (CI, Reactive Black 5), or Vilmafix® Green RR-4B (CI, Reactive Green). Plates were incubated for 72 h at15, 20 or 25°C, depending on the yeast isolate. Dye decolorization haloes and colony dyeing were recorded daily and used to select themost promising isolates. Of 139 isolates, 34% were classified as psychrophilic (growing only at 15°C) while the remaining 66% wereclassified as psychrotolerant (growing at all the assayed temperatures), irrespective of the medium assayed. Forty-five isolates producehaloes in at least one of the tested dyes in NDM, while only 40 yeast produced haloes in YM. 15 isolates were selected for further studiesIsolates A075 and Y28D produced intense haloes without colony dyeing; isolates Y67, Y70 and Y75 produced intense haloes and get dyedonly at the edge of the colonies while the remaining ten isolates, A092, A099, A104, A105, A106, A107, Y6, Y59, Y73 and Y84, producedneat haloes with a significant colorization of the entire colonies. The obtained results prove the biotechnological potential of Antarcticyeasts, raising the possibility of designing more efficient dye-decolorizing methods at low temperatures.Fil: Pacheco, Mariana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Dominguez, Facundo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Martinez Alvarez, Lucas Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaXL Reunión Científica Anual de la Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213140Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica); XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 8-8CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2022/12/libro-resumenes-2022.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:40:28Zoai:ri.conicet.gov.ar:11336/213140instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:40:28.94CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
title Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
spellingShingle Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
Pacheco, Mariana Soledad
DECOLORATION
TEXTILE DYE
YEAST
ANTARCTICA
title_short Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
title_full Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
title_fullStr Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
title_full_unstemmed Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
title_sort Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica)
dc.creator.none.fl_str_mv Pacheco, Mariana Soledad
Dominguez, Facundo Gabriel
Torres, Mariela Analía
Martorell, María Martha
Ruberto, Lucas Adolfo Mauro
Mac Cormack, Walter Patricio
Martinez Alvarez, Lucas Manuel
Pajot, Hipolito Fernando
Nieto Peñalver, Carlos Gabriel
author Pacheco, Mariana Soledad
author_facet Pacheco, Mariana Soledad
Dominguez, Facundo Gabriel
Torres, Mariela Analía
Martorell, María Martha
Ruberto, Lucas Adolfo Mauro
Mac Cormack, Walter Patricio
Martinez Alvarez, Lucas Manuel
Pajot, Hipolito Fernando
Nieto Peñalver, Carlos Gabriel
author_role author
author2 Dominguez, Facundo Gabriel
Torres, Mariela Analía
Martorell, María Martha
Ruberto, Lucas Adolfo Mauro
Mac Cormack, Walter Patricio
Martinez Alvarez, Lucas Manuel
Pajot, Hipolito Fernando
Nieto Peñalver, Carlos Gabriel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DECOLORATION
TEXTILE DYE
YEAST
ANTARCTICA
topic DECOLORATION
TEXTILE DYE
YEAST
ANTARCTICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Antarctic environments typically present low temperatures, high solar radiation and low nutrient availability, being one of the harshestenvironments on Earth. Psychrophilic and psychrotolerant yeasts from Antarctic soils and cryoconites have adapted to such conditions,being an interesting source of new enzymes with great biotechnological potential. This work intends to prove the textile dye decolorizingpotential of 139 yeasts isolated from soil and cryoconite samples from 25 de Mayo / King George Island, Antarctica. Isolates were cultivatedin YM, and NDM (Normal Decolorization Medium) media at 15, 20 and 25°C, and classified into psychrophilic and psychrotolerantaccording to their growth profiles. Textile dye decolorization was evaluated in the same two media, plus 200 mg/L of one of fourcommercially available reactive azo dyes: Vilmafix® Blue RR-BB (CI, Reactive Blue 221), Vilmafix® Red 7B-HE (CI, Reactive Red141), Vilmafix® Black B-V (CI, Reactive Black 5), or Vilmafix® Green RR-4B (CI, Reactive Green). Plates were incubated for 72 h at15, 20 or 25°C, depending on the yeast isolate. Dye decolorization haloes and colony dyeing were recorded daily and used to select themost promising isolates. Of 139 isolates, 34% were classified as psychrophilic (growing only at 15°C) while the remaining 66% wereclassified as psychrotolerant (growing at all the assayed temperatures), irrespective of the medium assayed. Forty-five isolates producehaloes in at least one of the tested dyes in NDM, while only 40 yeast produced haloes in YM. 15 isolates were selected for further studiesIsolates A075 and Y28D produced intense haloes without colony dyeing; isolates Y67, Y70 and Y75 produced intense haloes and get dyedonly at the edge of the colonies while the remaining ten isolates, A092, A099, A104, A105, A106, A107, Y6, Y59, Y73 and Y84, producedneat haloes with a significant colorization of the entire colonies. The obtained results prove the biotechnological potential of Antarcticyeasts, raising the possibility of designing more efficient dye-decolorizing methods at low temperatures.
Fil: Pacheco, Mariana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Dominguez, Facundo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Martinez Alvarez, Lucas Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XL Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
description Antarctic environments typically present low temperatures, high solar radiation and low nutrient availability, being one of the harshestenvironments on Earth. Psychrophilic and psychrotolerant yeasts from Antarctic soils and cryoconites have adapted to such conditions,being an interesting source of new enzymes with great biotechnological potential. This work intends to prove the textile dye decolorizingpotential of 139 yeasts isolated from soil and cryoconite samples from 25 de Mayo / King George Island, Antarctica. Isolates were cultivatedin YM, and NDM (Normal Decolorization Medium) media at 15, 20 and 25°C, and classified into psychrophilic and psychrotolerantaccording to their growth profiles. Textile dye decolorization was evaluated in the same two media, plus 200 mg/L of one of fourcommercially available reactive azo dyes: Vilmafix® Blue RR-BB (CI, Reactive Blue 221), Vilmafix® Red 7B-HE (CI, Reactive Red141), Vilmafix® Black B-V (CI, Reactive Black 5), or Vilmafix® Green RR-4B (CI, Reactive Green). Plates were incubated for 72 h at15, 20 or 25°C, depending on the yeast isolate. Dye decolorization haloes and colony dyeing were recorded daily and used to select themost promising isolates. Of 139 isolates, 34% were classified as psychrophilic (growing only at 15°C) while the remaining 66% wereclassified as psychrotolerant (growing at all the assayed temperatures), irrespective of the medium assayed. Forty-five isolates producehaloes in at least one of the tested dyes in NDM, while only 40 yeast produced haloes in YM. 15 isolates were selected for further studiesIsolates A075 and Y28D produced intense haloes without colony dyeing; isolates Y67, Y70 and Y75 produced intense haloes and get dyedonly at the edge of the colonies while the remaining ten isolates, A092, A099, A104, A105, A106, A107, Y6, Y59, Y73 and Y84, producedneat haloes with a significant colorization of the entire colonies. The obtained results prove the biotechnological potential of Antarcticyeasts, raising the possibility of designing more efficient dye-decolorizing methods at low temperatures.
publishDate 2023
dc.date.none.fl_str_mv 2023
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213140
Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica); XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 8-8
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213140
identifier_str_mv Assessing the textile-dye decolorization potential of psychrophilic and psychrotolerant yeasts from 25 de mayo/King George island (Antarctica); XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2022; 8-8
CONICET Digital
CONICET
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