Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina

Autores
Sannazzaro, Analía Inés; Torres Tejerizo, Gonzalo Arturo; Fontana, María Florencia; Cumpa Velázquez, Liz; Hansen, Lars H.; Pistorio, Mariano; Estrella, Maria Julia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two rhizobial strains, BSA136T and BSA150, related to the genus Mesorhizobium were isolated from root nodules of Lotus tenuis grown in saline-alkaline lowlands soil from Argentina. These strains showed different repetitive element palindromic PCR fingerprinting patterns but shared more than 99 % sequence similarity for both 16S rRNA and recA genes. Despite the symbiotic nodC gene sequences of our strains being related to the canonical Lotus biovar species comprising Mesorhizobium loti and Mesorhizobium japonicum, the 16S rRNA phylogenetic marker suggests that their taxonomical identities are closely related to Mesorhizobium helmanticense, Mesorhizobium metallidurans, Mesorhizobium thianshanense, Mesorhizobium gobiense and Mesorhizobium tarimense. Multilocus sequence analysis performed with seven housekeeping genes confirmed that BSA136T belongs to a separate clade within the genus Mesorhizobium. The results of comparisons for in silico DNA–DNA hybridization and average nucleotide identity indexes between the genomes of BSA136T and closest-related Mesorhizobium species were below the threshold for species delineation. Phenotypic features differentiated BSA136T from its closestrelated species. On the basis of our results, BSA136T and BSA150 can be considered to represent a novel species of the genus Mesorhizobium, for which the name Mesorhizobium sanjuanii sp. nov. is hereby proposed. The type strain of this species is BSA136T (=CECT 9305T =LMG 30060T ), for which the draft genome sequence is available.
Fil: Sannazzaro, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Fontana, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Cumpa Velázquez, Liz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Hansen, Lars H.. University Aarhus; Dinamarca
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Estrella, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
LOTUS TENUIS
MESORHIZOBIUM
NEW SPECIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100257
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, ArgentinaSannazzaro, Analía InésTorres Tejerizo, Gonzalo ArturoFontana, María FlorenciaCumpa Velázquez, LizHansen, Lars H.Pistorio, MarianoEstrella, Maria JuliaLOTUS TENUISMESORHIZOBIUMNEW SPECIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two rhizobial strains, BSA136T and BSA150, related to the genus Mesorhizobium were isolated from root nodules of Lotus tenuis grown in saline-alkaline lowlands soil from Argentina. These strains showed different repetitive element palindromic PCR fingerprinting patterns but shared more than 99 % sequence similarity for both 16S rRNA and recA genes. Despite the symbiotic nodC gene sequences of our strains being related to the canonical Lotus biovar species comprising Mesorhizobium loti and Mesorhizobium japonicum, the 16S rRNA phylogenetic marker suggests that their taxonomical identities are closely related to Mesorhizobium helmanticense, Mesorhizobium metallidurans, Mesorhizobium thianshanense, Mesorhizobium gobiense and Mesorhizobium tarimense. Multilocus sequence analysis performed with seven housekeeping genes confirmed that BSA136T belongs to a separate clade within the genus Mesorhizobium. The results of comparisons for in silico DNA–DNA hybridization and average nucleotide identity indexes between the genomes of BSA136T and closest-related Mesorhizobium species were below the threshold for species delineation. Phenotypic features differentiated BSA136T from its closestrelated species. On the basis of our results, BSA136T and BSA150 can be considered to represent a novel species of the genus Mesorhizobium, for which the name Mesorhizobium sanjuanii sp. nov. is hereby proposed. The type strain of this species is BSA136T (=CECT 9305T =LMG 30060T ), for which the draft genome sequence is available.Fil: Sannazzaro, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Fontana, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Cumpa Velázquez, Liz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Hansen, Lars H.. University Aarhus; DinamarcaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Estrella, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaSociety for General Microbiology2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100257Sannazzaro, Analía Inés; Torres Tejerizo, Gonzalo Arturo; Fontana, María Florencia; Cumpa Velázquez, Liz; Hansen, Lars H.; et al.; Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 68; 9; 9-2018; 2936-29421466-5026CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.002924info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002924info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:38Zoai:ri.conicet.gov.ar:11336/100257instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:39.094CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
title Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
spellingShingle Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
Sannazzaro, Analía Inés
LOTUS TENUIS
MESORHIZOBIUM
NEW SPECIES
title_short Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
title_full Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
title_fullStr Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
title_full_unstemmed Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
title_sort Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina
dc.creator.none.fl_str_mv Sannazzaro, Analía Inés
Torres Tejerizo, Gonzalo Arturo
Fontana, María Florencia
Cumpa Velázquez, Liz
Hansen, Lars H.
Pistorio, Mariano
Estrella, Maria Julia
author Sannazzaro, Analía Inés
author_facet Sannazzaro, Analía Inés
Torres Tejerizo, Gonzalo Arturo
Fontana, María Florencia
Cumpa Velázquez, Liz
Hansen, Lars H.
Pistorio, Mariano
Estrella, Maria Julia
author_role author
author2 Torres Tejerizo, Gonzalo Arturo
Fontana, María Florencia
Cumpa Velázquez, Liz
Hansen, Lars H.
Pistorio, Mariano
Estrella, Maria Julia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv LOTUS TENUIS
MESORHIZOBIUM
NEW SPECIES
topic LOTUS TENUIS
MESORHIZOBIUM
NEW SPECIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two rhizobial strains, BSA136T and BSA150, related to the genus Mesorhizobium were isolated from root nodules of Lotus tenuis grown in saline-alkaline lowlands soil from Argentina. These strains showed different repetitive element palindromic PCR fingerprinting patterns but shared more than 99 % sequence similarity for both 16S rRNA and recA genes. Despite the symbiotic nodC gene sequences of our strains being related to the canonical Lotus biovar species comprising Mesorhizobium loti and Mesorhizobium japonicum, the 16S rRNA phylogenetic marker suggests that their taxonomical identities are closely related to Mesorhizobium helmanticense, Mesorhizobium metallidurans, Mesorhizobium thianshanense, Mesorhizobium gobiense and Mesorhizobium tarimense. Multilocus sequence analysis performed with seven housekeeping genes confirmed that BSA136T belongs to a separate clade within the genus Mesorhizobium. The results of comparisons for in silico DNA–DNA hybridization and average nucleotide identity indexes between the genomes of BSA136T and closest-related Mesorhizobium species were below the threshold for species delineation. Phenotypic features differentiated BSA136T from its closestrelated species. On the basis of our results, BSA136T and BSA150 can be considered to represent a novel species of the genus Mesorhizobium, for which the name Mesorhizobium sanjuanii sp. nov. is hereby proposed. The type strain of this species is BSA136T (=CECT 9305T =LMG 30060T ), for which the draft genome sequence is available.
Fil: Sannazzaro, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Fontana, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Cumpa Velázquez, Liz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Hansen, Lars H.. University Aarhus; Dinamarca
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Estrella, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
description Two rhizobial strains, BSA136T and BSA150, related to the genus Mesorhizobium were isolated from root nodules of Lotus tenuis grown in saline-alkaline lowlands soil from Argentina. These strains showed different repetitive element palindromic PCR fingerprinting patterns but shared more than 99 % sequence similarity for both 16S rRNA and recA genes. Despite the symbiotic nodC gene sequences of our strains being related to the canonical Lotus biovar species comprising Mesorhizobium loti and Mesorhizobium japonicum, the 16S rRNA phylogenetic marker suggests that their taxonomical identities are closely related to Mesorhizobium helmanticense, Mesorhizobium metallidurans, Mesorhizobium thianshanense, Mesorhizobium gobiense and Mesorhizobium tarimense. Multilocus sequence analysis performed with seven housekeeping genes confirmed that BSA136T belongs to a separate clade within the genus Mesorhizobium. The results of comparisons for in silico DNA–DNA hybridization and average nucleotide identity indexes between the genomes of BSA136T and closest-related Mesorhizobium species were below the threshold for species delineation. Phenotypic features differentiated BSA136T from its closestrelated species. On the basis of our results, BSA136T and BSA150 can be considered to represent a novel species of the genus Mesorhizobium, for which the name Mesorhizobium sanjuanii sp. nov. is hereby proposed. The type strain of this species is BSA136T (=CECT 9305T =LMG 30060T ), for which the draft genome sequence is available.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100257
Sannazzaro, Analía Inés; Torres Tejerizo, Gonzalo Arturo; Fontana, María Florencia; Cumpa Velázquez, Liz; Hansen, Lars H.; et al.; Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 68; 9; 9-2018; 2936-2942
1466-5026
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100257
identifier_str_mv Sannazzaro, Analía Inés; Torres Tejerizo, Gonzalo Arturo; Fontana, María Florencia; Cumpa Velázquez, Liz; Hansen, Lars H.; et al.; Mesorhizobium sanjuanii sp. Nov., isolated from nodules of lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 68; 9; 9-2018; 2936-2942
1466-5026
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.002924
info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002924
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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