Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin

Autores
Pérez Serrano, Jorge; Grossmann, Claudio; Urrea París, María Ángeles; Denegri, Guillermo Maria; Casado, Nieves Maria Claudia; Rodriguez Caabeiro, Filomena
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The nematocidal activity of ivermectin (IVM) largely arises from its activity as a potent agonist of muscular and neuronal glutamate-gated chloride channels. A cestocidal effect has also been suggested following in vitro treatments, but the molecular basis of this activity is not clear. We studied the effect of IVM on the metacestode stage of the tapeworm Echinococcus granulosus by assessing the viability, ultrastructure, and tegumental membrane potential as a function of drug concentration and incubation time. Concentrations of 0.1 and 1.0 μg/ml of IVM had no effect on any of these three parameters for up to 6 days of treatment. A concentration of 10 μg/ml, however, elicited a sequence of alterations that started with a ∼20-mV depolarization of the tegumental membrane, and was followed by rostellar disorganization, rigid paralysis and, eventually, loss of viability. It is likely that the IVM-induced depolarization of the tegument acts as the signal that initiates the cascade of degenerative processes that leads to the parasite's death. This would place the tegument as the primary target of action of IVM on cestodes. As an appropriate chemotherapy for the hydatid disease is still lacking, the cestocidal effect of IVM reported here is worth considering.
Fil: Pérez Serrano, Jorge. Universidad de Alcalá; España
Fil: Grossmann, Claudio. State University of New York; Estados Unidos
Fil: Urrea París, María Ángeles. Universidad de Alcalá; España
Fil: Denegri, Guillermo Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Casado, Nieves Maria Claudia. Universidad de Alcalá; España
Fil: Rodriguez Caabeiro, Filomena. Universidad de Alcalá; España
Materia
Membrane Potential
Drug Concentration
Incubation Time
Molecular Basis
Primary Target
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40111
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectinPérez Serrano, JorgeGrossmann, ClaudioUrrea París, María ÁngelesDenegri, Guillermo MariaCasado, Nieves Maria ClaudiaRodriguez Caabeiro, FilomenaMembrane PotentialDrug ConcentrationIncubation TimeMolecular BasisPrimary Targethttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The nematocidal activity of ivermectin (IVM) largely arises from its activity as a potent agonist of muscular and neuronal glutamate-gated chloride channels. A cestocidal effect has also been suggested following in vitro treatments, but the molecular basis of this activity is not clear. We studied the effect of IVM on the metacestode stage of the tapeworm Echinococcus granulosus by assessing the viability, ultrastructure, and tegumental membrane potential as a function of drug concentration and incubation time. Concentrations of 0.1 and 1.0 μg/ml of IVM had no effect on any of these three parameters for up to 6 days of treatment. A concentration of 10 μg/ml, however, elicited a sequence of alterations that started with a ∼20-mV depolarization of the tegumental membrane, and was followed by rostellar disorganization, rigid paralysis and, eventually, loss of viability. It is likely that the IVM-induced depolarization of the tegument acts as the signal that initiates the cascade of degenerative processes that leads to the parasite's death. This would place the tegument as the primary target of action of IVM on cestodes. As an appropriate chemotherapy for the hydatid disease is still lacking, the cestocidal effect of IVM reported here is worth considering.Fil: Pérez Serrano, Jorge. Universidad de Alcalá; EspañaFil: Grossmann, Claudio. State University of New York; Estados UnidosFil: Urrea París, María Ángeles. Universidad de Alcalá; EspañaFil: Denegri, Guillermo Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Casado, Nieves Maria Claudia. Universidad de Alcalá; EspañaFil: Rodriguez Caabeiro, Filomena. Universidad de Alcalá; EspañaSpringer2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40111Pérez Serrano, Jorge; Grossmann, Claudio; Urrea París, María Ángeles; Denegri, Guillermo Maria; Casado, Nieves Maria Claudia; et al.; Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin; Springer; Parasitology Research; 87; 10; 12-2001; 804-8070932-0113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs004360100435info:eu-repo/semantics/altIdentifier/doi/10.1007/s004360100435info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:28Zoai:ri.conicet.gov.ar:11336/40111instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:28.555CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
title Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
spellingShingle Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
Pérez Serrano, Jorge
Membrane Potential
Drug Concentration
Incubation Time
Molecular Basis
Primary Target
title_short Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
title_full Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
title_fullStr Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
title_full_unstemmed Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
title_sort Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin
dc.creator.none.fl_str_mv Pérez Serrano, Jorge
Grossmann, Claudio
Urrea París, María Ángeles
Denegri, Guillermo Maria
Casado, Nieves Maria Claudia
Rodriguez Caabeiro, Filomena
author Pérez Serrano, Jorge
author_facet Pérez Serrano, Jorge
Grossmann, Claudio
Urrea París, María Ángeles
Denegri, Guillermo Maria
Casado, Nieves Maria Claudia
Rodriguez Caabeiro, Filomena
author_role author
author2 Grossmann, Claudio
Urrea París, María Ángeles
Denegri, Guillermo Maria
Casado, Nieves Maria Claudia
Rodriguez Caabeiro, Filomena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Membrane Potential
Drug Concentration
Incubation Time
Molecular Basis
Primary Target
topic Membrane Potential
Drug Concentration
Incubation Time
Molecular Basis
Primary Target
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The nematocidal activity of ivermectin (IVM) largely arises from its activity as a potent agonist of muscular and neuronal glutamate-gated chloride channels. A cestocidal effect has also been suggested following in vitro treatments, but the molecular basis of this activity is not clear. We studied the effect of IVM on the metacestode stage of the tapeworm Echinococcus granulosus by assessing the viability, ultrastructure, and tegumental membrane potential as a function of drug concentration and incubation time. Concentrations of 0.1 and 1.0 μg/ml of IVM had no effect on any of these three parameters for up to 6 days of treatment. A concentration of 10 μg/ml, however, elicited a sequence of alterations that started with a ∼20-mV depolarization of the tegumental membrane, and was followed by rostellar disorganization, rigid paralysis and, eventually, loss of viability. It is likely that the IVM-induced depolarization of the tegument acts as the signal that initiates the cascade of degenerative processes that leads to the parasite's death. This would place the tegument as the primary target of action of IVM on cestodes. As an appropriate chemotherapy for the hydatid disease is still lacking, the cestocidal effect of IVM reported here is worth considering.
Fil: Pérez Serrano, Jorge. Universidad de Alcalá; España
Fil: Grossmann, Claudio. State University of New York; Estados Unidos
Fil: Urrea París, María Ángeles. Universidad de Alcalá; España
Fil: Denegri, Guillermo Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Casado, Nieves Maria Claudia. Universidad de Alcalá; España
Fil: Rodriguez Caabeiro, Filomena. Universidad de Alcalá; España
description The nematocidal activity of ivermectin (IVM) largely arises from its activity as a potent agonist of muscular and neuronal glutamate-gated chloride channels. A cestocidal effect has also been suggested following in vitro treatments, but the molecular basis of this activity is not clear. We studied the effect of IVM on the metacestode stage of the tapeworm Echinococcus granulosus by assessing the viability, ultrastructure, and tegumental membrane potential as a function of drug concentration and incubation time. Concentrations of 0.1 and 1.0 μg/ml of IVM had no effect on any of these three parameters for up to 6 days of treatment. A concentration of 10 μg/ml, however, elicited a sequence of alterations that started with a ∼20-mV depolarization of the tegumental membrane, and was followed by rostellar disorganization, rigid paralysis and, eventually, loss of viability. It is likely that the IVM-induced depolarization of the tegument acts as the signal that initiates the cascade of degenerative processes that leads to the parasite's death. This would place the tegument as the primary target of action of IVM on cestodes. As an appropriate chemotherapy for the hydatid disease is still lacking, the cestocidal effect of IVM reported here is worth considering.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40111
Pérez Serrano, Jorge; Grossmann, Claudio; Urrea París, María Ángeles; Denegri, Guillermo Maria; Casado, Nieves Maria Claudia; et al.; Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin; Springer; Parasitology Research; 87; 10; 12-2001; 804-807
0932-0113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40111
identifier_str_mv Pérez Serrano, Jorge; Grossmann, Claudio; Urrea París, María Ángeles; Denegri, Guillermo Maria; Casado, Nieves Maria Claudia; et al.; Depolarization of the tegument precedes morphological alterations in Echinococcus granulosus protoscoleces incubated with ivermectin; Springer; Parasitology Research; 87; 10; 12-2001; 804-807
0932-0113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs004360100435
info:eu-repo/semantics/altIdentifier/doi/10.1007/s004360100435
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613672301756416
score 13.070432

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