MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces

Autores
Mohammadi, Mohammad Ali; Mansouri, Mehdi; Derakhshani, Ali; Rezaie, Masoud; Borhani, Mehdi; Nasibi, Saeid; Mousavi, Seyed Mohammad; Afgar, Ali; Macchiaroli, Natalia; Rosenzvit, Mara Cecilia; Harandi, Majid Fasihi
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Echinococcus granulosus sensu lato has a complex developmental biology with a variety of factors relating to both intermediate and final hosts. To achieve maximum parasite adaptability, the development of the cestode is dependent on essential changes in transcript regulation. Transcription factors (TFs) and miRNAs are known as master regulators that affect the expression of downstream genes through a wide range of metabolic and signaling pathways. In this study, we aimed to develop a regulatory miRNA-Transcription factor (miRNA-TF) network across early developmental stages of E. granulosus protoscoleces by performing in silico analysis, and to experimentally validate TFs expression in protoscoleces obtained from in vitro culture, and from in vivo experiments. Results: We obtained list of 394 unique E. granulosus TFs and matched them with 818 differentially expressed genes which identified 41 predicted TFs with differential expression. These TFs were used to predict the potential targets of 31 differentially expressed miRNAs. As a result, eight miRNAs and eight TFs were found, and the predicted network was constructed using Cytoscape. At least four miRNAs (egr-miR-124a, egr-miR-124b-3p, egr-miR-745-3p, and egr-miR-87-3p) and their corresponding differentially expressed TFs (Zinc finger protein 45, Early growth response protein 3, Ecdysone induced protein 78c and ETS transcription factor elf 2) were highlighted in this investigation. The expression of predicted differentially expressed TFs obtained from in vitro and in vivo experiments, were experimentally validated by quantitative polymerase chain reaction. This confirmed findings of RNA-seq data. Conclusion: miRNA-TF networks presented in this study control some of the most important metabolic and signaling pathways in the development and life cycle of E. granulosus, providing a potential approach for disrupting the early hours of dog infection and preventing the development of the helminth in the final host.
Fil: Mohammadi, Mohammad Ali. Kerman University of Medical Sciences; Irán
Fil: Mansouri, Mehdi. Shahid Bahonar University of Kerman; Irán
Fil: Derakhshani, Ali. Kerman University of Medical Sciences; Irán
Fil: Rezaie, Masoud. Kerman University of Medical Sciences; Irán
Fil: Borhani, Mehdi. Jilin University; China
Fil: Nasibi, Saeid. Kerman University of Medical Sciences; Irán
Fil: Mousavi, Seyed Mohammad. Kerman University of Medical Sciences; Irán
Fil: Afgar, Ali. Kerman University of Medical Sciences; Irán
Fil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Harandi, Majid Fasihi. Kerman University of Medical Sciences; Irán
Materia
CANINE ECHINOCOCCOSIS
DOG INTESTINE
ECHINOCOCCUS GRANULOSUS SENSU STRICTO
GENE REGULATION
PEPSIN
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227986
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227986
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscolecesMohammadi, Mohammad AliMansouri, MehdiDerakhshani, AliRezaie, MasoudBorhani, MehdiNasibi, SaeidMousavi, Seyed MohammadAfgar, AliMacchiaroli, NataliaRosenzvit, Mara CeciliaHarandi, Majid FasihiCANINE ECHINOCOCCOSISDOG INTESTINEECHINOCOCCUS GRANULOSUS SENSU STRICTOGENE REGULATIONPEPSINTRANSCRIPTOMEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Echinococcus granulosus sensu lato has a complex developmental biology with a variety of factors relating to both intermediate and final hosts. To achieve maximum parasite adaptability, the development of the cestode is dependent on essential changes in transcript regulation. Transcription factors (TFs) and miRNAs are known as master regulators that affect the expression of downstream genes through a wide range of metabolic and signaling pathways. In this study, we aimed to develop a regulatory miRNA-Transcription factor (miRNA-TF) network across early developmental stages of E. granulosus protoscoleces by performing in silico analysis, and to experimentally validate TFs expression in protoscoleces obtained from in vitro culture, and from in vivo experiments. Results: We obtained list of 394 unique E. granulosus TFs and matched them with 818 differentially expressed genes which identified 41 predicted TFs with differential expression. These TFs were used to predict the potential targets of 31 differentially expressed miRNAs. As a result, eight miRNAs and eight TFs were found, and the predicted network was constructed using Cytoscape. At least four miRNAs (egr-miR-124a, egr-miR-124b-3p, egr-miR-745-3p, and egr-miR-87-3p) and their corresponding differentially expressed TFs (Zinc finger protein 45, Early growth response protein 3, Ecdysone induced protein 78c and ETS transcription factor elf 2) were highlighted in this investigation. The expression of predicted differentially expressed TFs obtained from in vitro and in vivo experiments, were experimentally validated by quantitative polymerase chain reaction. This confirmed findings of RNA-seq data. Conclusion: miRNA-TF networks presented in this study control some of the most important metabolic and signaling pathways in the development and life cycle of E. granulosus, providing a potential approach for disrupting the early hours of dog infection and preventing the development of the helminth in the final host.Fil: Mohammadi, Mohammad Ali. Kerman University of Medical Sciences; IránFil: Mansouri, Mehdi. Shahid Bahonar University of Kerman; IránFil: Derakhshani, Ali. Kerman University of Medical Sciences; IránFil: Rezaie, Masoud. Kerman University of Medical Sciences; IránFil: Borhani, Mehdi. Jilin University; ChinaFil: Nasibi, Saeid. Kerman University of Medical Sciences; IránFil: Mousavi, Seyed Mohammad. Kerman University of Medical Sciences; IránFil: Afgar, Ali. Kerman University of Medical Sciences; IránFil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Harandi, Majid Fasihi. Kerman University of Medical Sciences; IránBioMed Central2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227986Mohammadi, Mohammad Ali; Mansouri, Mehdi; Derakhshani, Ali; Rezaie, Masoud; Borhani, Mehdi; et al.; MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces; BioMed Central; BMC Genomics; 24; 1; 3-2023; 1-131471-2164CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-023-09199-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:29Zoai:ri.conicet.gov.ar:11336/227986instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:29.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
title MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
spellingShingle MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
Mohammadi, Mohammad Ali
CANINE ECHINOCOCCOSIS
DOG INTESTINE
ECHINOCOCCUS GRANULOSUS SENSU STRICTO
GENE REGULATION
PEPSIN
TRANSCRIPTOME
title_short MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
title_full MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
title_fullStr MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
title_full_unstemmed MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
title_sort MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces
dc.creator.none.fl_str_mv Mohammadi, Mohammad Ali
Mansouri, Mehdi
Derakhshani, Ali
Rezaie, Masoud
Borhani, Mehdi
Nasibi, Saeid
Mousavi, Seyed Mohammad
Afgar, Ali
Macchiaroli, Natalia
Rosenzvit, Mara Cecilia
Harandi, Majid Fasihi
author Mohammadi, Mohammad Ali
author_facet Mohammadi, Mohammad Ali
Mansouri, Mehdi
Derakhshani, Ali
Rezaie, Masoud
Borhani, Mehdi
Nasibi, Saeid
Mousavi, Seyed Mohammad
Afgar, Ali
Macchiaroli, Natalia
Rosenzvit, Mara Cecilia
Harandi, Majid Fasihi
author_role author
author2 Mansouri, Mehdi
Derakhshani, Ali
Rezaie, Masoud
Borhani, Mehdi
Nasibi, Saeid
Mousavi, Seyed Mohammad
Afgar, Ali
Macchiaroli, Natalia
Rosenzvit, Mara Cecilia
Harandi, Majid Fasihi
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CANINE ECHINOCOCCOSIS
DOG INTESTINE
ECHINOCOCCUS GRANULOSUS SENSU STRICTO
GENE REGULATION
PEPSIN
TRANSCRIPTOME
topic CANINE ECHINOCOCCOSIS
DOG INTESTINE
ECHINOCOCCUS GRANULOSUS SENSU STRICTO
GENE REGULATION
PEPSIN
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Echinococcus granulosus sensu lato has a complex developmental biology with a variety of factors relating to both intermediate and final hosts. To achieve maximum parasite adaptability, the development of the cestode is dependent on essential changes in transcript regulation. Transcription factors (TFs) and miRNAs are known as master regulators that affect the expression of downstream genes through a wide range of metabolic and signaling pathways. In this study, we aimed to develop a regulatory miRNA-Transcription factor (miRNA-TF) network across early developmental stages of E. granulosus protoscoleces by performing in silico analysis, and to experimentally validate TFs expression in protoscoleces obtained from in vitro culture, and from in vivo experiments. Results: We obtained list of 394 unique E. granulosus TFs and matched them with 818 differentially expressed genes which identified 41 predicted TFs with differential expression. These TFs were used to predict the potential targets of 31 differentially expressed miRNAs. As a result, eight miRNAs and eight TFs were found, and the predicted network was constructed using Cytoscape. At least four miRNAs (egr-miR-124a, egr-miR-124b-3p, egr-miR-745-3p, and egr-miR-87-3p) and their corresponding differentially expressed TFs (Zinc finger protein 45, Early growth response protein 3, Ecdysone induced protein 78c and ETS transcription factor elf 2) were highlighted in this investigation. The expression of predicted differentially expressed TFs obtained from in vitro and in vivo experiments, were experimentally validated by quantitative polymerase chain reaction. This confirmed findings of RNA-seq data. Conclusion: miRNA-TF networks presented in this study control some of the most important metabolic and signaling pathways in the development and life cycle of E. granulosus, providing a potential approach for disrupting the early hours of dog infection and preventing the development of the helminth in the final host.
Fil: Mohammadi, Mohammad Ali. Kerman University of Medical Sciences; Irán
Fil: Mansouri, Mehdi. Shahid Bahonar University of Kerman; Irán
Fil: Derakhshani, Ali. Kerman University of Medical Sciences; Irán
Fil: Rezaie, Masoud. Kerman University of Medical Sciences; Irán
Fil: Borhani, Mehdi. Jilin University; China
Fil: Nasibi, Saeid. Kerman University of Medical Sciences; Irán
Fil: Mousavi, Seyed Mohammad. Kerman University of Medical Sciences; Irán
Fil: Afgar, Ali. Kerman University of Medical Sciences; Irán
Fil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Harandi, Majid Fasihi. Kerman University of Medical Sciences; Irán
description Background: Echinococcus granulosus sensu lato has a complex developmental biology with a variety of factors relating to both intermediate and final hosts. To achieve maximum parasite adaptability, the development of the cestode is dependent on essential changes in transcript regulation. Transcription factors (TFs) and miRNAs are known as master regulators that affect the expression of downstream genes through a wide range of metabolic and signaling pathways. In this study, we aimed to develop a regulatory miRNA-Transcription factor (miRNA-TF) network across early developmental stages of E. granulosus protoscoleces by performing in silico analysis, and to experimentally validate TFs expression in protoscoleces obtained from in vitro culture, and from in vivo experiments. Results: We obtained list of 394 unique E. granulosus TFs and matched them with 818 differentially expressed genes which identified 41 predicted TFs with differential expression. These TFs were used to predict the potential targets of 31 differentially expressed miRNAs. As a result, eight miRNAs and eight TFs were found, and the predicted network was constructed using Cytoscape. At least four miRNAs (egr-miR-124a, egr-miR-124b-3p, egr-miR-745-3p, and egr-miR-87-3p) and their corresponding differentially expressed TFs (Zinc finger protein 45, Early growth response protein 3, Ecdysone induced protein 78c and ETS transcription factor elf 2) were highlighted in this investigation. The expression of predicted differentially expressed TFs obtained from in vitro and in vivo experiments, were experimentally validated by quantitative polymerase chain reaction. This confirmed findings of RNA-seq data. Conclusion: miRNA-TF networks presented in this study control some of the most important metabolic and signaling pathways in the development and life cycle of E. granulosus, providing a potential approach for disrupting the early hours of dog infection and preventing the development of the helminth in the final host.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227986
Mohammadi, Mohammad Ali; Mansouri, Mehdi; Derakhshani, Ali; Rezaie, Masoud; Borhani, Mehdi; et al.; MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces; BioMed Central; BMC Genomics; 24; 1; 3-2023; 1-13
1471-2164
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227986
identifier_str_mv Mohammadi, Mohammad Ali; Mansouri, Mehdi; Derakhshani, Ali; Rezaie, Masoud; Borhani, Mehdi; et al.; MicroRNA-Transcription factor regulatory networks in the early strobilar development of Echinococcus granulosus protoscoleces; BioMed Central; BMC Genomics; 24; 1; 3-2023; 1-13
1471-2164
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-023-09199-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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