Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production

Autores
Lam, Philip Y.; Yin, Fei; Hamilton, Ryan T.; Boveris, Alberto Antonio; Cadenas, Enrique
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study evaluated the effect of ageing on brain mitochondrial function mediated through protein post-translational modifications. Neuronal nitric oxide synthase increased with age and this led to a discreet pattern of nitration of mitochondrial proteins. LC/MS/MS analyses identified the nitrated mitochondrial proteins as succinyl-CoA-transferase and F1-ATPase; the latter was nitrated at Tyr269, suggesting deficient ADP binding to the active site. Activities of succinyl-CoA-transferase, F1-ATPase and cytochrome oxidase decreased with age. The decreased activity of the latter cannot be ascribed to protein modifications and is most likely due to a decreased expression and assembly of complex IV. Mitochondrial protein post-translational modifications were associated with a moderately impaired mitochondrial function, as indicated by the decreased respiratory control ratios as a function of age and by the release of mitochondrial cytochrome c to the cytosol, thus supporting the amplification of apoptotic cascades.
Fil: Lam, Philip Y.. University of Southern California; Estados Unidos
Fil: Yin, Fei. University of Southern California; Estados Unidos
Fil: Hamilton, Ryan T.. University of Southern California; Estados Unidos
Fil: Boveris, Alberto Antonio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cadenas, Enrique. University of Southern California; Estados Unidos
Materia
mtNOS
NITRATION
AGEING
F1-ATPase
SUCCINYL-CoA TRANSFERASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113756
Acceder
id CONICETDig_a1549e23e87601ee36f10c75b36f4237
oai_identifier_str oai:ri.conicet.gov.ar:11336/113756
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy productionLam, Philip Y.Yin, FeiHamilton, Ryan T.Boveris, Alberto AntonioCadenas, EnriquemtNOSNITRATIONAGEINGF1-ATPaseSUCCINYL-CoA TRANSFERASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study evaluated the effect of ageing on brain mitochondrial function mediated through protein post-translational modifications. Neuronal nitric oxide synthase increased with age and this led to a discreet pattern of nitration of mitochondrial proteins. LC/MS/MS analyses identified the nitrated mitochondrial proteins as succinyl-CoA-transferase and F1-ATPase; the latter was nitrated at Tyr269, suggesting deficient ADP binding to the active site. Activities of succinyl-CoA-transferase, F1-ATPase and cytochrome oxidase decreased with age. The decreased activity of the latter cannot be ascribed to protein modifications and is most likely due to a decreased expression and assembly of complex IV. Mitochondrial protein post-translational modifications were associated with a moderately impaired mitochondrial function, as indicated by the decreased respiratory control ratios as a function of age and by the release of mitochondrial cytochrome c to the cytosol, thus supporting the amplification of apoptotic cascades.Fil: Lam, Philip Y.. University of Southern California; Estados UnidosFil: Yin, Fei. University of Southern California; Estados UnidosFil: Hamilton, Ryan T.. University of Southern California; Estados UnidosFil: Boveris, Alberto Antonio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Cadenas, Enrique. University of Southern California; Estados UnidosTaylor & Francis Ltd2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113756Lam, Philip Y.; Yin, Fei; Hamilton, Ryan T.; Boveris, Alberto Antonio; Cadenas, Enrique; Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production; Taylor & Francis Ltd; Free Radical Research; 43; 5; 9-2009; 431-4391071-5762CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10715760902849813info:eu-repo/semantics/altIdentifier/doi/10.1080/10715760902849813info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:29Zoai:ri.conicet.gov.ar:11336/113756instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:29.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
title Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
spellingShingle Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
Lam, Philip Y.
mtNOS
NITRATION
AGEING
F1-ATPase
SUCCINYL-CoA TRANSFERASE
title_short Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
title_full Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
title_fullStr Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
title_full_unstemmed Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
title_sort Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production
dc.creator.none.fl_str_mv Lam, Philip Y.
Yin, Fei
Hamilton, Ryan T.
Boveris, Alberto Antonio
Cadenas, Enrique
author Lam, Philip Y.
author_facet Lam, Philip Y.
Yin, Fei
Hamilton, Ryan T.
Boveris, Alberto Antonio
Cadenas, Enrique
author_role author
author2 Yin, Fei
Hamilton, Ryan T.
Boveris, Alberto Antonio
Cadenas, Enrique
author2_role author
author
author
author
dc.subject.none.fl_str_mv mtNOS
NITRATION
AGEING
F1-ATPase
SUCCINYL-CoA TRANSFERASE
topic mtNOS
NITRATION
AGEING
F1-ATPase
SUCCINYL-CoA TRANSFERASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study evaluated the effect of ageing on brain mitochondrial function mediated through protein post-translational modifications. Neuronal nitric oxide synthase increased with age and this led to a discreet pattern of nitration of mitochondrial proteins. LC/MS/MS analyses identified the nitrated mitochondrial proteins as succinyl-CoA-transferase and F1-ATPase; the latter was nitrated at Tyr269, suggesting deficient ADP binding to the active site. Activities of succinyl-CoA-transferase, F1-ATPase and cytochrome oxidase decreased with age. The decreased activity of the latter cannot be ascribed to protein modifications and is most likely due to a decreased expression and assembly of complex IV. Mitochondrial protein post-translational modifications were associated with a moderately impaired mitochondrial function, as indicated by the decreased respiratory control ratios as a function of age and by the release of mitochondrial cytochrome c to the cytosol, thus supporting the amplification of apoptotic cascades.
Fil: Lam, Philip Y.. University of Southern California; Estados Unidos
Fil: Yin, Fei. University of Southern California; Estados Unidos
Fil: Hamilton, Ryan T.. University of Southern California; Estados Unidos
Fil: Boveris, Alberto Antonio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cadenas, Enrique. University of Southern California; Estados Unidos
description This study evaluated the effect of ageing on brain mitochondrial function mediated through protein post-translational modifications. Neuronal nitric oxide synthase increased with age and this led to a discreet pattern of nitration of mitochondrial proteins. LC/MS/MS analyses identified the nitrated mitochondrial proteins as succinyl-CoA-transferase and F1-ATPase; the latter was nitrated at Tyr269, suggesting deficient ADP binding to the active site. Activities of succinyl-CoA-transferase, F1-ATPase and cytochrome oxidase decreased with age. The decreased activity of the latter cannot be ascribed to protein modifications and is most likely due to a decreased expression and assembly of complex IV. Mitochondrial protein post-translational modifications were associated with a moderately impaired mitochondrial function, as indicated by the decreased respiratory control ratios as a function of age and by the release of mitochondrial cytochrome c to the cytosol, thus supporting the amplification of apoptotic cascades.
publishDate 2009
dc.date.none.fl_str_mv 2009-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113756
Lam, Philip Y.; Yin, Fei; Hamilton, Ryan T.; Boveris, Alberto Antonio; Cadenas, Enrique; Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production; Taylor & Francis Ltd; Free Radical Research; 43; 5; 9-2009; 431-439
1071-5762
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113756
identifier_str_mv Lam, Philip Y.; Yin, Fei; Hamilton, Ryan T.; Boveris, Alberto Antonio; Cadenas, Enrique; Elevated neuronal nitric oxide synthase expression during ageing and mitochondrial energy production; Taylor & Francis Ltd; Free Radical Research; 43; 5; 9-2009; 431-439
1071-5762
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10715760902849813
info:eu-repo/semantics/altIdentifier/doi/10.1080/10715760902849813
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613829859737600
score 13.070432

Publicaciones similares