Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments

Autores
Zaobornyj, Tamara; Valdez, Laura Batriz; Iglesias, Dario Ezequiel; Gasco, Manuel; Gonzales, Gustavo F.; Boveris, Alberto Antonio
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, L-NAME, and L-arginine treatments. Am J Physiol Heart Circ Physiol 296: H1741–H1747, 2009. First published April 3, 2009; doi:10.1152/ajpheart.00422.2008.—Rats submitted to high altitude (Cerro de Pasco, Peru´, 4,340 m, PO2 12.2 kPa) for up to 84 days showed a physiological adaptive response with decreased body weight gain (15%), increased right ventricle weight (100%), and increased hematocrit (40%) compared with sea level animals. These classical parameters of adaptation to high altitude were accompanied by an increase in heart mitochondrial enzymes: complexes I-III activity by 34% and mitochondrial nitric oxide synthase (mtNOS) activity and expression by 75%. The hyperbolic increase for mtNOS activity during adaptation to high altitude was similar to the observed pattern for hematocrit. Hematocrit and mtNOS activity mean values correlated linearly (r2 0.75, P 0.05). Chronic treatment for 28 days with sildenafil (50 mg kg1 day1 ) decreased the response of mtNOS to high altitude by 25%. Conversely, NGnitro-L-arginine methyl ester treatment (8.3 mg kg1 day1 ) increased such response by 40%, whereas L-arginine treatment (106 mg kg1 day1 ) had no effect. Nitric oxide (NO) production by mtNOS accounts for 49% of total cellular NO production in sea level rats and for 54% in rats exposed to high altitude for 84 days. It is concluded that mtNOS is a substantial source of cardiac NO, a factor in the adaptive response to sustained heart hypoxia that is susceptible to be modified by pharmacological treatments.
Fil: Zaobornyj, Tamara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valdez, Laura Batriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias, Dario Ezequiel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gasco, Manuel. Universidad Peruana Cayetano Heredia; Perú
Fil: Gonzales, Gustavo F.. Universidad Peruana Cayetano Heredia; Perú
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
mtNOS ACTIVITY
mtNOS EXPRESSION
MITOCHONDRIAL RESPIRATORY COMPLEXES
HEMATOCRIT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102551
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102551
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatmentsZaobornyj, TamaraValdez, Laura BatrizIglesias, Dario EzequielGasco, ManuelGonzales, Gustavo F.Boveris, Alberto AntoniomtNOS ACTIVITYmtNOS EXPRESSIONMITOCHONDRIAL RESPIRATORY COMPLEXESHEMATOCRIThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, L-NAME, and L-arginine treatments. Am J Physiol Heart Circ Physiol 296: H1741–H1747, 2009. First published April 3, 2009; doi:10.1152/ajpheart.00422.2008.—Rats submitted to high altitude (Cerro de Pasco, Peru´, 4,340 m, PO2 12.2 kPa) for up to 84 days showed a physiological adaptive response with decreased body weight gain (15%), increased right ventricle weight (100%), and increased hematocrit (40%) compared with sea level animals. These classical parameters of adaptation to high altitude were accompanied by an increase in heart mitochondrial enzymes: complexes I-III activity by 34% and mitochondrial nitric oxide synthase (mtNOS) activity and expression by 75%. The hyperbolic increase for mtNOS activity during adaptation to high altitude was similar to the observed pattern for hematocrit. Hematocrit and mtNOS activity mean values correlated linearly (r2 0.75, P 0.05). Chronic treatment for 28 days with sildenafil (50 mg kg1 day1 ) decreased the response of mtNOS to high altitude by 25%. Conversely, NGnitro-L-arginine methyl ester treatment (8.3 mg kg1 day1 ) increased such response by 40%, whereas L-arginine treatment (106 mg kg1 day1 ) had no effect. Nitric oxide (NO) production by mtNOS accounts for 49% of total cellular NO production in sea level rats and for 54% in rats exposed to high altitude for 84 days. It is concluded that mtNOS is a substantial source of cardiac NO, a factor in the adaptive response to sustained heart hypoxia that is susceptible to be modified by pharmacological treatments.Fil: Zaobornyj, Tamara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valdez, Laura Batriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iglesias, Dario Ezequiel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gasco, Manuel. Universidad Peruana Cayetano Heredia; PerúFil: Gonzales, Gustavo F.. Universidad Peruana Cayetano Heredia; PerúFil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaAmerican Physiological Society2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102551Zaobornyj, Tamara; Valdez, Laura Batriz; Iglesias, Dario Ezequiel; Gasco, Manuel; Gonzales, Gustavo F.; et al.; Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments; American Physiological Society; American Journal of Physiology - Heart and Circulatory Physiology; 296; 6; 6-2009; H1741-H17470363-6135CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1152/ajpheart.00422.2008info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/19346458/info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpheart.00422.2008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:24:49Zoai:ri.conicet.gov.ar:11336/102551instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:24:49.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
title Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
spellingShingle Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
Zaobornyj, Tamara
mtNOS ACTIVITY
mtNOS EXPRESSION
MITOCHONDRIAL RESPIRATORY COMPLEXES
HEMATOCRIT
title_short Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
title_full Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
title_fullStr Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
title_full_unstemmed Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
title_sort Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments
dc.creator.none.fl_str_mv Zaobornyj, Tamara
Valdez, Laura Batriz
Iglesias, Dario Ezequiel
Gasco, Manuel
Gonzales, Gustavo F.
Boveris, Alberto Antonio
author Zaobornyj, Tamara
author_facet Zaobornyj, Tamara
Valdez, Laura Batriz
Iglesias, Dario Ezequiel
Gasco, Manuel
Gonzales, Gustavo F.
Boveris, Alberto Antonio
author_role author
author2 Valdez, Laura Batriz
Iglesias, Dario Ezequiel
Gasco, Manuel
Gonzales, Gustavo F.
Boveris, Alberto Antonio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv mtNOS ACTIVITY
mtNOS EXPRESSION
MITOCHONDRIAL RESPIRATORY COMPLEXES
HEMATOCRIT
topic mtNOS ACTIVITY
mtNOS EXPRESSION
MITOCHONDRIAL RESPIRATORY COMPLEXES
HEMATOCRIT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, L-NAME, and L-arginine treatments. Am J Physiol Heart Circ Physiol 296: H1741–H1747, 2009. First published April 3, 2009; doi:10.1152/ajpheart.00422.2008.—Rats submitted to high altitude (Cerro de Pasco, Peru´, 4,340 m, PO2 12.2 kPa) for up to 84 days showed a physiological adaptive response with decreased body weight gain (15%), increased right ventricle weight (100%), and increased hematocrit (40%) compared with sea level animals. These classical parameters of adaptation to high altitude were accompanied by an increase in heart mitochondrial enzymes: complexes I-III activity by 34% and mitochondrial nitric oxide synthase (mtNOS) activity and expression by 75%. The hyperbolic increase for mtNOS activity during adaptation to high altitude was similar to the observed pattern for hematocrit. Hematocrit and mtNOS activity mean values correlated linearly (r2 0.75, P 0.05). Chronic treatment for 28 days with sildenafil (50 mg kg1 day1 ) decreased the response of mtNOS to high altitude by 25%. Conversely, NGnitro-L-arginine methyl ester treatment (8.3 mg kg1 day1 ) increased such response by 40%, whereas L-arginine treatment (106 mg kg1 day1 ) had no effect. Nitric oxide (NO) production by mtNOS accounts for 49% of total cellular NO production in sea level rats and for 54% in rats exposed to high altitude for 84 days. It is concluded that mtNOS is a substantial source of cardiac NO, a factor in the adaptive response to sustained heart hypoxia that is susceptible to be modified by pharmacological treatments.
Fil: Zaobornyj, Tamara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valdez, Laura Batriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias, Dario Ezequiel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gasco, Manuel. Universidad Peruana Cayetano Heredia; Perú
Fil: Gonzales, Gustavo F.. Universidad Peruana Cayetano Heredia; Perú
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, L-NAME, and L-arginine treatments. Am J Physiol Heart Circ Physiol 296: H1741–H1747, 2009. First published April 3, 2009; doi:10.1152/ajpheart.00422.2008.—Rats submitted to high altitude (Cerro de Pasco, Peru´, 4,340 m, PO2 12.2 kPa) for up to 84 days showed a physiological adaptive response with decreased body weight gain (15%), increased right ventricle weight (100%), and increased hematocrit (40%) compared with sea level animals. These classical parameters of adaptation to high altitude were accompanied by an increase in heart mitochondrial enzymes: complexes I-III activity by 34% and mitochondrial nitric oxide synthase (mtNOS) activity and expression by 75%. The hyperbolic increase for mtNOS activity during adaptation to high altitude was similar to the observed pattern for hematocrit. Hematocrit and mtNOS activity mean values correlated linearly (r2 0.75, P 0.05). Chronic treatment for 28 days with sildenafil (50 mg kg1 day1 ) decreased the response of mtNOS to high altitude by 25%. Conversely, NGnitro-L-arginine methyl ester treatment (8.3 mg kg1 day1 ) increased such response by 40%, whereas L-arginine treatment (106 mg kg1 day1 ) had no effect. Nitric oxide (NO) production by mtNOS accounts for 49% of total cellular NO production in sea level rats and for 54% in rats exposed to high altitude for 84 days. It is concluded that mtNOS is a substantial source of cardiac NO, a factor in the adaptive response to sustained heart hypoxia that is susceptible to be modified by pharmacological treatments.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102551
Zaobornyj, Tamara; Valdez, Laura Batriz; Iglesias, Dario Ezequiel; Gasco, Manuel; Gonzales, Gustavo F.; et al.; Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments; American Physiological Society; American Journal of Physiology - Heart and Circulatory Physiology; 296; 6; 6-2009; H1741-H1747
0363-6135
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102551
identifier_str_mv Zaobornyj, Tamara; Valdez, Laura Batriz; Iglesias, Dario Ezequiel; Gasco, Manuel; Gonzales, Gustavo F.; et al.; Mitochondrial nitric oxide metabolism during rat heart adaptation to high altitude: effect of sildenafil, l -NAME, and l -arginine treatments; American Physiological Society; American Journal of Physiology - Heart and Circulatory Physiology; 296; 6; 6-2009; H1741-H1747
0363-6135
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpheart.00422.2008
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/19346458/
info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpheart.00422.2008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082674707398656
score 13.22299

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