Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
- Autores
- Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; Romero, H.; Santos, E.; Zunino, P.; Antúnez, Karina
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health.
Fil: Castelli, L.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Branchiccela, B.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Invernizzi, Ciro. No especifíca;
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Romero, H.. No especifíca;
Fil: Santos, E.. No especifíca;
Fil: Zunino, P.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay - Materia
-
APIS MELLIFERA
COLONY LOSSES
MICROBIOTA
NUTRITIONAL STRESS
PATHOGENS
POLLEN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/174735
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CONICET Digital (CONICET) |
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Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae InfectionCastelli, L.Branchiccela, B.Garrido, Paula MelisaInvernizzi, CiroPorrini, Martín PabloRomero, H.Santos, E.Zunino, P.Antúnez, KarinaAPIS MELLIFERACOLONY LOSSESMICROBIOTANUTRITIONAL STRESSPATHOGENSPOLLENhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health.Fil: Castelli, L.. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Branchiccela, B.. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Invernizzi, Ciro. No especifíca;Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Romero, H.. No especifíca;Fil: Santos, E.. No especifíca;Fil: Zunino, P.. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguaySpringer2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174735Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; et al.; Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection; Springer; Microbial Ecology; 80; 4; 7-2020; 908-9190095-3628CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00248-020-01538-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00248-020-01538-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:30Zoai:ri.conicet.gov.ar:11336/174735instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:30.542CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection |
title |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection |
spellingShingle |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection Castelli, L. APIS MELLIFERA COLONY LOSSES MICROBIOTA NUTRITIONAL STRESS PATHOGENS POLLEN |
title_short |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection |
title_full |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection |
title_fullStr |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection |
title_full_unstemmed |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection |
title_sort |
Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection |
dc.creator.none.fl_str_mv |
Castelli, L. Branchiccela, B. Garrido, Paula Melisa Invernizzi, Ciro Porrini, Martín Pablo Romero, H. Santos, E. Zunino, P. Antúnez, Karina |
author |
Castelli, L. |
author_facet |
Castelli, L. Branchiccela, B. Garrido, Paula Melisa Invernizzi, Ciro Porrini, Martín Pablo Romero, H. Santos, E. Zunino, P. Antúnez, Karina |
author_role |
author |
author2 |
Branchiccela, B. Garrido, Paula Melisa Invernizzi, Ciro Porrini, Martín Pablo Romero, H. Santos, E. Zunino, P. Antúnez, Karina |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
APIS MELLIFERA COLONY LOSSES MICROBIOTA NUTRITIONAL STRESS PATHOGENS POLLEN |
topic |
APIS MELLIFERA COLONY LOSSES MICROBIOTA NUTRITIONAL STRESS PATHOGENS POLLEN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health. Fil: Castelli, L.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Branchiccela, B.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina Fil: Invernizzi, Ciro. No especifíca; Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina Fil: Romero, H.. No especifíca; Fil: Santos, E.. No especifíca; Fil: Zunino, P.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay |
description |
Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/174735 Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; et al.; Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection; Springer; Microbial Ecology; 80; 4; 7-2020; 908-919 0095-3628 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/174735 |
identifier_str_mv |
Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; et al.; Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection; Springer; Microbial Ecology; 80; 4; 7-2020; 908-919 0095-3628 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00248-020-01538-1 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00248-020-01538-1 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614241578909696 |
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13.070432 |