Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection

Autores
Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; Romero, H.; Santos, E.; Zunino, P.; Antúnez, Karina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health.
Fil: Castelli, L.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Branchiccela, B.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Invernizzi, Ciro. No especifíca;
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Romero, H.. No especifíca;
Fil: Santos, E.. No especifíca;
Fil: Zunino, P.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Materia
APIS MELLIFERA
COLONY LOSSES
MICROBIOTA
NUTRITIONAL STRESS
PATHOGENS
POLLEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/174735
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/174735
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae InfectionCastelli, L.Branchiccela, B.Garrido, Paula MelisaInvernizzi, CiroPorrini, Martín PabloRomero, H.Santos, E.Zunino, P.Antúnez, KarinaAPIS MELLIFERACOLONY LOSSESMICROBIOTANUTRITIONAL STRESSPATHOGENSPOLLENhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health.Fil: Castelli, L.. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Branchiccela, B.. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Invernizzi, Ciro. No especifíca;Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Romero, H.. No especifíca;Fil: Santos, E.. No especifíca;Fil: Zunino, P.. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguaySpringer2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/174735Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; et al.; Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection; Springer; Microbial Ecology; 80; 4; 7-2020; 908-9190095-3628CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00248-020-01538-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00248-020-01538-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:57:13Zoai:ri.conicet.gov.ar:11336/174735instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:57:14.228CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
title Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
spellingShingle Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
Castelli, L.
APIS MELLIFERA
COLONY LOSSES
MICROBIOTA
NUTRITIONAL STRESS
PATHOGENS
POLLEN
title_short Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
title_full Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
title_fullStr Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
title_full_unstemmed Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
title_sort Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection
dc.creator.none.fl_str_mv Castelli, L.
Branchiccela, B.
Garrido, Paula Melisa
Invernizzi, Ciro
Porrini, Martín Pablo
Romero, H.
Santos, E.
Zunino, P.
Antúnez, Karina
author Castelli, L.
author_facet Castelli, L.
Branchiccela, B.
Garrido, Paula Melisa
Invernizzi, Ciro
Porrini, Martín Pablo
Romero, H.
Santos, E.
Zunino, P.
Antúnez, Karina
author_role author
author2 Branchiccela, B.
Garrido, Paula Melisa
Invernizzi, Ciro
Porrini, Martín Pablo
Romero, H.
Santos, E.
Zunino, P.
Antúnez, Karina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APIS MELLIFERA
COLONY LOSSES
MICROBIOTA
NUTRITIONAL STRESS
PATHOGENS
POLLEN
topic APIS MELLIFERA
COLONY LOSSES
MICROBIOTA
NUTRITIONAL STRESS
PATHOGENS
POLLEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health.
Fil: Castelli, L.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Branchiccela, B.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Garrido, Paula Melisa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Invernizzi, Ciro. No especifíca;
Fil: Porrini, Martín Pablo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Romero, H.. No especifíca;
Fil: Santos, E.. No especifíca;
Fil: Zunino, P.. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
Fil: Antúnez, Karina. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
description Honeybees are important pollinators, having an essential role in the ecology of natural and agricultural environments. Honeybee colony losses episodes reported worldwide and have been associated with different pests and pathogens, pesticide exposure, and nutritional stress. This nutritional stress is related to the increase in monoculture areas which leads to a reduction of pollen availability and diversity. In this study, we examined whether nutritional stress affects honeybee gut microbiota, bee immunity, and infection by Nosema ceranae, under laboratory conditions. Consumption of Eucalyptus grandis pollen was used as a nutritionally poor-quality diet to study nutritional stress, in contraposition to the consumption of polyfloral pollen. Honeybees feed with Eucalyptus grandis pollen showed a lower abundance of Lactobacillus mellifer and Lactobacillus apis (Firm-4 and Firm-5, respectively) and Bifidobacterium spp. and a higher abundance of Bartonella apis, than honeybees fed with polyfloral pollen. Besides the impact of nutritional stress on honeybee microbiota, it also decreased the expression levels of vitellogenin and genes associated to immunity (glucose oxidase, hymenoptaecin and lysozyme). Finally, Eucalyptus grandis pollen favored the multiplication of Nosema ceranae. These results show that nutritional stress impacts the honeybee gut microbiota, having consequences on honeybee immunity and pathogen development. Those results may be useful to understand the influence of modern agriculture on honeybee health.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/174735
Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; et al.; Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection; Springer; Microbial Ecology; 80; 4; 7-2020; 908-919
0095-3628
CONICET Digital
CONICET
url http://hdl.handle.net/11336/174735
identifier_str_mv Castelli, L.; Branchiccela, B.; Garrido, Paula Melisa; Invernizzi, Ciro; Porrini, Martín Pablo; et al.; Impact of Nutritional Stress on Honeybee Gut Microbiota, Immunity, and Nosema ceranae Infection; Springer; Microbial Ecology; 80; 4; 7-2020; 908-919
0095-3628
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00248-020-01538-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00248-020-01538-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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