Chemical constituents and biological activity of Euglena gracilis extracts

Autores
Tolivia, Analía Alejandra; Conforti, Visitacion Teresa D.; Córdoba, Osvaldo León; Flores, Maria Lujan
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements,suggesting the existence of diverse physiological patterns. The aim of this work is to carry out a study about secondary metabolites biosynthesis on two strains of E. gracilis cultured in vitro. Methods: Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolated from an urban polluted river (MAT) were screened for preliminary identification of chemical constituents. Both strains were studied in their photosynthetic and bleached forms, on their exponential and stationary growth phases. Chromatographic analysis of pigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition, and toxic activity were tested in vitro. Results: The phytochemical analysis of extracts indicated the presence of steroids in all samples, cardenolids and triterpenes in the exponential growth phase. With the exception of the photosynthetic MAT strain, tannins were present in all the other on exponential phase samples and flavonoids were only observed in the stationary phase of both photosynthetic strains. Chromatographic profiles show that chlorophyll content decreased while carotenoids content increased in the stationary phase of both photosynthetic strains, and reveal the presence of flavonols derived from quercetin. In concordance with the presence of polyphenols, the fractions with the highest polarity showed antioxidant activity against DPPH and growth inhibition activity in vitro even in the absence of paramylon, previously reported to have antitumoral properties. Conclusion: This work constitutes the first report about polyphenol production in Euglenoids, which allows us a first assessment of the potential of E. gracilis as a source of bioactive products.
Fil: Tolivia, Analía Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Conforti, Visitacion Teresa D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Córdoba, Osvaldo León. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Flores, Maria Lujan. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Materia
ANTIOXIDANT ACTIVITY
EUGLENA GRACILIS
GROWTH INHIBITION ACTIVITY
PHYTOCHEMICAL SCREENING
POLYPHENOLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85079
Acceder
id CONICETDig_a05654bfc09612bf5240c59cb9c5bc57
oai_identifier_str oai:ri.conicet.gov.ar:11336/85079
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chemical constituents and biological activity of Euglena gracilis extractsTolivia, Analía AlejandraConforti, Visitacion Teresa D.Córdoba, Osvaldo LeónFlores, Maria LujanANTIOXIDANT ACTIVITYEUGLENA GRACILISGROWTH INHIBITION ACTIVITYPHYTOCHEMICAL SCREENINGPOLYPHENOLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements,suggesting the existence of diverse physiological patterns. The aim of this work is to carry out a study about secondary metabolites biosynthesis on two strains of E. gracilis cultured in vitro. Methods: Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolated from an urban polluted river (MAT) were screened for preliminary identification of chemical constituents. Both strains were studied in their photosynthetic and bleached forms, on their exponential and stationary growth phases. Chromatographic analysis of pigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition, and toxic activity were tested in vitro. Results: The phytochemical analysis of extracts indicated the presence of steroids in all samples, cardenolids and triterpenes in the exponential growth phase. With the exception of the photosynthetic MAT strain, tannins were present in all the other on exponential phase samples and flavonoids were only observed in the stationary phase of both photosynthetic strains. Chromatographic profiles show that chlorophyll content decreased while carotenoids content increased in the stationary phase of both photosynthetic strains, and reveal the presence of flavonols derived from quercetin. In concordance with the presence of polyphenols, the fractions with the highest polarity showed antioxidant activity against DPPH and growth inhibition activity in vitro even in the absence of paramylon, previously reported to have antitumoral properties. Conclusion: This work constitutes the first report about polyphenol production in Euglenoids, which allows us a first assessment of the potential of E. gracilis as a source of bioactive products.Fil: Tolivia, Analía Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Conforti, Visitacion Teresa D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Córdoba, Osvaldo León. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Flores, Maria Lujan. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaElsevier2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85079Tolivia, Analía Alejandra; Conforti, Visitacion Teresa D.; Córdoba, Osvaldo León; Flores, Maria Lujan; Chemical constituents and biological activity of Euglena gracilis extracts; Elsevier; Journal of Pharmacy Research; 7; 5-2013; 209-2140974-6943CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jopr.2013.03.019info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S097469431300128Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:30Zoai:ri.conicet.gov.ar:11336/85079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:30.956CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chemical constituents and biological activity of Euglena gracilis extracts
title Chemical constituents and biological activity of Euglena gracilis extracts
spellingShingle Chemical constituents and biological activity of Euglena gracilis extracts
Tolivia, Analía Alejandra
ANTIOXIDANT ACTIVITY
EUGLENA GRACILIS
GROWTH INHIBITION ACTIVITY
PHYTOCHEMICAL SCREENING
POLYPHENOLS
title_short Chemical constituents and biological activity of Euglena gracilis extracts
title_full Chemical constituents and biological activity of Euglena gracilis extracts
title_fullStr Chemical constituents and biological activity of Euglena gracilis extracts
title_full_unstemmed Chemical constituents and biological activity of Euglena gracilis extracts
title_sort Chemical constituents and biological activity of Euglena gracilis extracts
dc.creator.none.fl_str_mv Tolivia, Analía Alejandra
Conforti, Visitacion Teresa D.
Córdoba, Osvaldo León
Flores, Maria Lujan
author Tolivia, Analía Alejandra
author_facet Tolivia, Analía Alejandra
Conforti, Visitacion Teresa D.
Córdoba, Osvaldo León
Flores, Maria Lujan
author_role author
author2 Conforti, Visitacion Teresa D.
Córdoba, Osvaldo León
Flores, Maria Lujan
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT ACTIVITY
EUGLENA GRACILIS
GROWTH INHIBITION ACTIVITY
PHYTOCHEMICAL SCREENING
POLYPHENOLS
topic ANTIOXIDANT ACTIVITY
EUGLENA GRACILIS
GROWTH INHIBITION ACTIVITY
PHYTOCHEMICAL SCREENING
POLYPHENOLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements,suggesting the existence of diverse physiological patterns. The aim of this work is to carry out a study about secondary metabolites biosynthesis on two strains of E. gracilis cultured in vitro. Methods: Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolated from an urban polluted river (MAT) were screened for preliminary identification of chemical constituents. Both strains were studied in their photosynthetic and bleached forms, on their exponential and stationary growth phases. Chromatographic analysis of pigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition, and toxic activity were tested in vitro. Results: The phytochemical analysis of extracts indicated the presence of steroids in all samples, cardenolids and triterpenes in the exponential growth phase. With the exception of the photosynthetic MAT strain, tannins were present in all the other on exponential phase samples and flavonoids were only observed in the stationary phase of both photosynthetic strains. Chromatographic profiles show that chlorophyll content decreased while carotenoids content increased in the stationary phase of both photosynthetic strains, and reveal the presence of flavonols derived from quercetin. In concordance with the presence of polyphenols, the fractions with the highest polarity showed antioxidant activity against DPPH and growth inhibition activity in vitro even in the absence of paramylon, previously reported to have antitumoral properties. Conclusion: This work constitutes the first report about polyphenol production in Euglenoids, which allows us a first assessment of the potential of E. gracilis as a source of bioactive products.
Fil: Tolivia, Analía Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Conforti, Visitacion Teresa D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Córdoba, Osvaldo León. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Flores, Maria Lujan. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
description Background: Euglena gracilis is a microalgae with a wide range of nutritional requirements,suggesting the existence of diverse physiological patterns. The aim of this work is to carry out a study about secondary metabolites biosynthesis on two strains of E. gracilis cultured in vitro. Methods: Extracts from a Euglena gracilis (Klebs) commercial strain and a wild type isolated from an urban polluted river (MAT) were screened for preliminary identification of chemical constituents. Both strains were studied in their photosynthetic and bleached forms, on their exponential and stationary growth phases. Chromatographic analysis of pigments, lipids, and flavonoids were performed. Besides antioxidant, growth inhibition, and toxic activity were tested in vitro. Results: The phytochemical analysis of extracts indicated the presence of steroids in all samples, cardenolids and triterpenes in the exponential growth phase. With the exception of the photosynthetic MAT strain, tannins were present in all the other on exponential phase samples and flavonoids were only observed in the stationary phase of both photosynthetic strains. Chromatographic profiles show that chlorophyll content decreased while carotenoids content increased in the stationary phase of both photosynthetic strains, and reveal the presence of flavonols derived from quercetin. In concordance with the presence of polyphenols, the fractions with the highest polarity showed antioxidant activity against DPPH and growth inhibition activity in vitro even in the absence of paramylon, previously reported to have antitumoral properties. Conclusion: This work constitutes the first report about polyphenol production in Euglenoids, which allows us a first assessment of the potential of E. gracilis as a source of bioactive products.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85079
Tolivia, Analía Alejandra; Conforti, Visitacion Teresa D.; Córdoba, Osvaldo León; Flores, Maria Lujan; Chemical constituents and biological activity of Euglena gracilis extracts; Elsevier; Journal of Pharmacy Research; 7; 5-2013; 209-214
0974-6943
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85079
identifier_str_mv Tolivia, Analía Alejandra; Conforti, Visitacion Teresa D.; Córdoba, Osvaldo León; Flores, Maria Lujan; Chemical constituents and biological activity of Euglena gracilis extracts; Elsevier; Journal of Pharmacy Research; 7; 5-2013; 209-214
0974-6943
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jopr.2013.03.019
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S097469431300128X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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