PLAGL1 gene function during hepatoma cells proliferation

Autores
Vega Benedetti, Ana Florencia; Saucedo, Cinthia Natalia; Zavattari, Patrizia; Vanni, Roberta; Royo, Felix; LLavero, Freancisco; Zugaza, José L.; Parada, Luis Antonio
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hepatocellular carcinoma develops as a multistep process, in which cell cycle deregulation is a central feature, resulting in unscheduled proliferation. The PLAGL1 gene encodes a homonym zinc finger protein that is involved in cell-proliferation control. We determined the genomic profile and the transcription and expression level of PLAGL1, simultaneously with that of its molecular partners p53, PPARγ and p21, in cell-lines derived from patients with liver cancer, during in vitro cell growth. Our investigations revealed that genomic and epigenetic changes of PLAGL1 are also present in hepatoma cell-lines. Transcription of PLAGL1 in tumor cells is significantly lower than in normal fibroblasts, but no significant differences in terms of protein expression were detected between these two cell-types, indicating that there is not a direct relationship between the gene transcriptional activity and protein expression. RT-PCR analyses on normal fibroblasts, used as control, also showed that PLAGL1 and p53 genes transcription occurs as an apparent orchestrated process during normal cells proliferation, which gets disturbed in cancer cells. Furthermore, abnormal trafficking of the PLAGL1 protein may occur in hepatocarcinogenesis.
Fil: Vega Benedetti, Ana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Saucedo, Cinthia Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Zavattari, Patrizia. University of Cagliari; Italia
Fil: Vanni, Roberta. University of Cagliari; Italia
Fil: Royo, Felix. CIC BioGUNE CIBERehd; España
Fil: LLavero, Freancisco. Universidad del País Vasco; España. IKERBASQUE; España
Fil: Zugaza, José L.. Universidad del País Vasco; España. IKERBASQUE; España
Fil: Parada, Luis Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Materia
CELL PROLIFERATION
CHROMOSOME
HEPATOCELLULAR CARCINOMA
METHYLATION
PLAGL1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/86657
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/86657
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PLAGL1 gene function during hepatoma cells proliferationVega Benedetti, Ana FlorenciaSaucedo, Cinthia NataliaZavattari, PatriziaVanni, RobertaRoyo, FelixLLavero, FreanciscoZugaza, José L.Parada, Luis AntonioCELL PROLIFERATIONCHROMOSOMEHEPATOCELLULAR CARCINOMAMETHYLATIONPLAGL1https://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Hepatocellular carcinoma develops as a multistep process, in which cell cycle deregulation is a central feature, resulting in unscheduled proliferation. The PLAGL1 gene encodes a homonym zinc finger protein that is involved in cell-proliferation control. We determined the genomic profile and the transcription and expression level of PLAGL1, simultaneously with that of its molecular partners p53, PPARγ and p21, in cell-lines derived from patients with liver cancer, during in vitro cell growth. Our investigations revealed that genomic and epigenetic changes of PLAGL1 are also present in hepatoma cell-lines. Transcription of PLAGL1 in tumor cells is significantly lower than in normal fibroblasts, but no significant differences in terms of protein expression were detected between these two cell-types, indicating that there is not a direct relationship between the gene transcriptional activity and protein expression. RT-PCR analyses on normal fibroblasts, used as control, also showed that PLAGL1 and p53 genes transcription occurs as an apparent orchestrated process during normal cells proliferation, which gets disturbed in cancer cells. Furthermore, abnormal trafficking of the PLAGL1 protein may occur in hepatocarcinogenesis.Fil: Vega Benedetti, Ana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Saucedo, Cinthia Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Zavattari, Patrizia. University of Cagliari; ItaliaFil: Vanni, Roberta. University of Cagliari; ItaliaFil: Royo, Felix. CIC BioGUNE CIBERehd; EspañaFil: LLavero, Freancisco. Universidad del País Vasco; España. IKERBASQUE; EspañaFil: Zugaza, José L.. Universidad del País Vasco; España. IKERBASQUE; EspañaFil: Parada, Luis Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaImpact Journals LLC2018-08-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/86657Vega Benedetti, Ana Florencia; Saucedo, Cinthia Natalia; Zavattari, Patrizia; Vanni, Roberta; Royo, Felix; et al.; PLAGL1 gene function during hepatoma cells proliferation; Impact Journals LLC; Oncotarget; 9; 67; 28-8-2018; 32775-327941949-2553CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.25996info:eu-repo/semantics/altIdentifier/url/http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=25996&path[]=81113info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:57Zoai:ri.conicet.gov.ar:11336/86657instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:57.653CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PLAGL1 gene function during hepatoma cells proliferation
title PLAGL1 gene function during hepatoma cells proliferation
spellingShingle PLAGL1 gene function during hepatoma cells proliferation
Vega Benedetti, Ana Florencia
CELL PROLIFERATION
CHROMOSOME
HEPATOCELLULAR CARCINOMA
METHYLATION
PLAGL1
title_short PLAGL1 gene function during hepatoma cells proliferation
title_full PLAGL1 gene function during hepatoma cells proliferation
title_fullStr PLAGL1 gene function during hepatoma cells proliferation
title_full_unstemmed PLAGL1 gene function during hepatoma cells proliferation
title_sort PLAGL1 gene function during hepatoma cells proliferation
dc.creator.none.fl_str_mv Vega Benedetti, Ana Florencia
Saucedo, Cinthia Natalia
Zavattari, Patrizia
Vanni, Roberta
Royo, Felix
LLavero, Freancisco
Zugaza, José L.
Parada, Luis Antonio
author Vega Benedetti, Ana Florencia
author_facet Vega Benedetti, Ana Florencia
Saucedo, Cinthia Natalia
Zavattari, Patrizia
Vanni, Roberta
Royo, Felix
LLavero, Freancisco
Zugaza, José L.
Parada, Luis Antonio
author_role author
author2 Saucedo, Cinthia Natalia
Zavattari, Patrizia
Vanni, Roberta
Royo, Felix
LLavero, Freancisco
Zugaza, José L.
Parada, Luis Antonio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELL PROLIFERATION
CHROMOSOME
HEPATOCELLULAR CARCINOMA
METHYLATION
PLAGL1
topic CELL PROLIFERATION
CHROMOSOME
HEPATOCELLULAR CARCINOMA
METHYLATION
PLAGL1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Hepatocellular carcinoma develops as a multistep process, in which cell cycle deregulation is a central feature, resulting in unscheduled proliferation. The PLAGL1 gene encodes a homonym zinc finger protein that is involved in cell-proliferation control. We determined the genomic profile and the transcription and expression level of PLAGL1, simultaneously with that of its molecular partners p53, PPARγ and p21, in cell-lines derived from patients with liver cancer, during in vitro cell growth. Our investigations revealed that genomic and epigenetic changes of PLAGL1 are also present in hepatoma cell-lines. Transcription of PLAGL1 in tumor cells is significantly lower than in normal fibroblasts, but no significant differences in terms of protein expression were detected between these two cell-types, indicating that there is not a direct relationship between the gene transcriptional activity and protein expression. RT-PCR analyses on normal fibroblasts, used as control, also showed that PLAGL1 and p53 genes transcription occurs as an apparent orchestrated process during normal cells proliferation, which gets disturbed in cancer cells. Furthermore, abnormal trafficking of the PLAGL1 protein may occur in hepatocarcinogenesis.
Fil: Vega Benedetti, Ana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Saucedo, Cinthia Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Zavattari, Patrizia. University of Cagliari; Italia
Fil: Vanni, Roberta. University of Cagliari; Italia
Fil: Royo, Felix. CIC BioGUNE CIBERehd; España
Fil: LLavero, Freancisco. Universidad del País Vasco; España. IKERBASQUE; España
Fil: Zugaza, José L.. Universidad del País Vasco; España. IKERBASQUE; España
Fil: Parada, Luis Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
description Hepatocellular carcinoma develops as a multistep process, in which cell cycle deregulation is a central feature, resulting in unscheduled proliferation. The PLAGL1 gene encodes a homonym zinc finger protein that is involved in cell-proliferation control. We determined the genomic profile and the transcription and expression level of PLAGL1, simultaneously with that of its molecular partners p53, PPARγ and p21, in cell-lines derived from patients with liver cancer, during in vitro cell growth. Our investigations revealed that genomic and epigenetic changes of PLAGL1 are also present in hepatoma cell-lines. Transcription of PLAGL1 in tumor cells is significantly lower than in normal fibroblasts, but no significant differences in terms of protein expression were detected between these two cell-types, indicating that there is not a direct relationship between the gene transcriptional activity and protein expression. RT-PCR analyses on normal fibroblasts, used as control, also showed that PLAGL1 and p53 genes transcription occurs as an apparent orchestrated process during normal cells proliferation, which gets disturbed in cancer cells. Furthermore, abnormal trafficking of the PLAGL1 protein may occur in hepatocarcinogenesis.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/86657
Vega Benedetti, Ana Florencia; Saucedo, Cinthia Natalia; Zavattari, Patrizia; Vanni, Roberta; Royo, Felix; et al.; PLAGL1 gene function during hepatoma cells proliferation; Impact Journals LLC; Oncotarget; 9; 67; 28-8-2018; 32775-32794
1949-2553
CONICET Digital
CONICET
url http://hdl.handle.net/11336/86657
identifier_str_mv Vega Benedetti, Ana Florencia; Saucedo, Cinthia Natalia; Zavattari, Patrizia; Vanni, Roberta; Royo, Felix; et al.; PLAGL1 gene function during hepatoma cells proliferation; Impact Journals LLC; Oncotarget; 9; 67; 28-8-2018; 32775-32794
1949-2553
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.25996
info:eu-repo/semantics/altIdentifier/url/http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=25996&path[]=81113
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Impact Journals LLC
publisher.none.fl_str_mv Impact Journals LLC
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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