Differential role of AHCYL1 gene in tumor plasticity

Autores
Budnik, Nicolás; Muñoz Bernart, Melina Daniela; Manzi, Malena; Monge, Maria Eugenia; Espinoza, Joaquin M.; Mostoslavsky, Gustavo; Perez Castro, Carolina Ines
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Malignant reprogramming of cells is responsible for tumor development. During this process stem-like tumor cells that acquired self-renew capacity produce heterogeneity, tumor dissemination, and relapse after cancer therapy. We have previously identified AHCYL1 as a potential regulation target of core transcription factors OCT-4, SOX-2, and NANOG responsible for cell reprogramming. We studied AHCYL1 by analyzing its cellular location and expression levels during cell plasticity events of tumor cells. We used the glioblastoma (GBM) cell line U87 and lung carcinoma (LC) cell line H1299 as in vitro models since brain and lung have the highest Ahcyl1 expression. We cultured these cell lines in a 3D format in DMEM/F12 medium supplied with FGF, EGF, and B27 and compared with 2D format cultured cells with DMEM serum complemented medium. Ahcy1 localization was determined by immunofluorescence assay and cell fractioning followed by western blotting. To generate U87 and H1299 Ahcyl1 knockdown stable lines, three different shRNAs were tested and the expression levels of Ahcyl1 and the core factors were determined by Western blot and RT-qPCR. Stemness potency was evaluated by ELDA assay (extreme limiting dilution analysis). We found that AHCYL1 localizes both in nuclei and cytosol, in addition to a putative processed isoform in nuclei. In 3D cultures, Ahcyl1 expression is differently regulated compared to 2D cultures. Also, in GBM, AHCYL1 expression was significantly increased, in contrast in LC was decreased (p 0.0001 and p 0.05 respectively). In Ahcyl1-depleted LC cells, the core factors expression levels and the stem potency were increased (p 0.05). Altogether, we conclude Ahcyl1 has a key role as a regulator of stem potency and would be dependent on tumor type.
Fil: Budnik, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muñoz Bernart, Melina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Manzi, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Espinoza, Joaquin M.. University of Colorado; Estados Unidos
Fil: Mostoslavsky, Gustavo. University Of Boston. School Of Medicine. Center For Regenerative Medicine.; Estados Unidos
Fil: Perez Castro, Carolina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
Materia
Ahcyl1
GBM
Lung cancer
Cancer stem cell
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237931
Acceder
id CONICETDig_9fd1884cd9a7af6ea6279b1675323967
oai_identifier_str oai:ri.conicet.gov.ar:11336/237931
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential role of AHCYL1 gene in tumor plasticityBudnik, NicolásMuñoz Bernart, Melina DanielaManzi, MalenaMonge, Maria EugeniaEspinoza, Joaquin M.Mostoslavsky, GustavoPerez Castro, Carolina InesAhcyl1GBMLung cancerCancer stem cellhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Malignant reprogramming of cells is responsible for tumor development. During this process stem-like tumor cells that acquired self-renew capacity produce heterogeneity, tumor dissemination, and relapse after cancer therapy. We have previously identified AHCYL1 as a potential regulation target of core transcription factors OCT-4, SOX-2, and NANOG responsible for cell reprogramming. We studied AHCYL1 by analyzing its cellular location and expression levels during cell plasticity events of tumor cells. We used the glioblastoma (GBM) cell line U87 and lung carcinoma (LC) cell line H1299 as in vitro models since brain and lung have the highest Ahcyl1 expression. We cultured these cell lines in a 3D format in DMEM/F12 medium supplied with FGF, EGF, and B27 and compared with 2D format cultured cells with DMEM serum complemented medium. Ahcy1 localization was determined by immunofluorescence assay and cell fractioning followed by western blotting. To generate U87 and H1299 Ahcyl1 knockdown stable lines, three different shRNAs were tested and the expression levels of Ahcyl1 and the core factors were determined by Western blot and RT-qPCR. Stemness potency was evaluated by ELDA assay (extreme limiting dilution analysis). We found that AHCYL1 localizes both in nuclei and cytosol, in addition to a putative processed isoform in nuclei. In 3D cultures, Ahcyl1 expression is differently regulated compared to 2D cultures. Also, in GBM, AHCYL1 expression was significantly increased, in contrast in LC was decreased (p 0.0001 and p 0.05 respectively). In Ahcyl1-depleted LC cells, the core factors expression levels and the stem potency were increased (p 0.05). Altogether, we conclude Ahcyl1 has a key role as a regulator of stem potency and would be dependent on tumor type.Fil: Budnik, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Muñoz Bernart, Melina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Manzi, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; ArgentinaFil: Espinoza, Joaquin M.. University of Colorado; Estados UnidosFil: Mostoslavsky, Gustavo. University Of Boston. School Of Medicine. Center For Regenerative Medicine.; Estados UnidosFil: Perez Castro, Carolina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioThe Histochemical SocietyFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237931Differential role of AHCYL1 gene in tumor plasticity; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 91-921669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:00Zoai:ri.conicet.gov.ar:11336/237931instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:00.978CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential role of AHCYL1 gene in tumor plasticity
title Differential role of AHCYL1 gene in tumor plasticity
spellingShingle Differential role of AHCYL1 gene in tumor plasticity
Budnik, Nicolás
Ahcyl1
GBM
Lung cancer
Cancer stem cell
title_short Differential role of AHCYL1 gene in tumor plasticity
title_full Differential role of AHCYL1 gene in tumor plasticity
title_fullStr Differential role of AHCYL1 gene in tumor plasticity
title_full_unstemmed Differential role of AHCYL1 gene in tumor plasticity
title_sort Differential role of AHCYL1 gene in tumor plasticity
dc.creator.none.fl_str_mv Budnik, Nicolás
Muñoz Bernart, Melina Daniela
Manzi, Malena
Monge, Maria Eugenia
Espinoza, Joaquin M.
Mostoslavsky, Gustavo
Perez Castro, Carolina Ines
author Budnik, Nicolás
author_facet Budnik, Nicolás
Muñoz Bernart, Melina Daniela
Manzi, Malena
Monge, Maria Eugenia
Espinoza, Joaquin M.
Mostoslavsky, Gustavo
Perez Castro, Carolina Ines
author_role author
author2 Muñoz Bernart, Melina Daniela
Manzi, Malena
Monge, Maria Eugenia
Espinoza, Joaquin M.
Mostoslavsky, Gustavo
Perez Castro, Carolina Ines
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ahcyl1
GBM
Lung cancer
Cancer stem cell
topic Ahcyl1
GBM
Lung cancer
Cancer stem cell
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Malignant reprogramming of cells is responsible for tumor development. During this process stem-like tumor cells that acquired self-renew capacity produce heterogeneity, tumor dissemination, and relapse after cancer therapy. We have previously identified AHCYL1 as a potential regulation target of core transcription factors OCT-4, SOX-2, and NANOG responsible for cell reprogramming. We studied AHCYL1 by analyzing its cellular location and expression levels during cell plasticity events of tumor cells. We used the glioblastoma (GBM) cell line U87 and lung carcinoma (LC) cell line H1299 as in vitro models since brain and lung have the highest Ahcyl1 expression. We cultured these cell lines in a 3D format in DMEM/F12 medium supplied with FGF, EGF, and B27 and compared with 2D format cultured cells with DMEM serum complemented medium. Ahcy1 localization was determined by immunofluorescence assay and cell fractioning followed by western blotting. To generate U87 and H1299 Ahcyl1 knockdown stable lines, three different shRNAs were tested and the expression levels of Ahcyl1 and the core factors were determined by Western blot and RT-qPCR. Stemness potency was evaluated by ELDA assay (extreme limiting dilution analysis). We found that AHCYL1 localizes both in nuclei and cytosol, in addition to a putative processed isoform in nuclei. In 3D cultures, Ahcyl1 expression is differently regulated compared to 2D cultures. Also, in GBM, AHCYL1 expression was significantly increased, in contrast in LC was decreased (p 0.0001 and p 0.05 respectively). In Ahcyl1-depleted LC cells, the core factors expression levels and the stem potency were increased (p 0.05). Altogether, we conclude Ahcyl1 has a key role as a regulator of stem potency and would be dependent on tumor type.
Fil: Budnik, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muñoz Bernart, Melina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Manzi, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Espinoza, Joaquin M.. University of Colorado; Estados Unidos
Fil: Mostoslavsky, Gustavo. University Of Boston. School Of Medicine. Center For Regenerative Medicine.; Estados Unidos
Fil: Perez Castro, Carolina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
description Malignant reprogramming of cells is responsible for tumor development. During this process stem-like tumor cells that acquired self-renew capacity produce heterogeneity, tumor dissemination, and relapse after cancer therapy. We have previously identified AHCYL1 as a potential regulation target of core transcription factors OCT-4, SOX-2, and NANOG responsible for cell reprogramming. We studied AHCYL1 by analyzing its cellular location and expression levels during cell plasticity events of tumor cells. We used the glioblastoma (GBM) cell line U87 and lung carcinoma (LC) cell line H1299 as in vitro models since brain and lung have the highest Ahcyl1 expression. We cultured these cell lines in a 3D format in DMEM/F12 medium supplied with FGF, EGF, and B27 and compared with 2D format cultured cells with DMEM serum complemented medium. Ahcy1 localization was determined by immunofluorescence assay and cell fractioning followed by western blotting. To generate U87 and H1299 Ahcyl1 knockdown stable lines, three different shRNAs were tested and the expression levels of Ahcyl1 and the core factors were determined by Western blot and RT-qPCR. Stemness potency was evaluated by ELDA assay (extreme limiting dilution analysis). We found that AHCYL1 localizes both in nuclei and cytosol, in addition to a putative processed isoform in nuclei. In 3D cultures, Ahcyl1 expression is differently regulated compared to 2D cultures. Also, in GBM, AHCYL1 expression was significantly increased, in contrast in LC was decreased (p 0.0001 and p 0.05 respectively). In Ahcyl1-depleted LC cells, the core factors expression levels and the stem potency were increased (p 0.05). Altogether, we conclude Ahcyl1 has a key role as a regulator of stem potency and would be dependent on tumor type.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237931
Differential role of AHCYL1 gene in tumor plasticity; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 91-92
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237931
identifier_str_mv Differential role of AHCYL1 gene in tumor plasticity; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 91-92
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613519601827840
score 13.070432

Publicaciones similares