Androgen Deprivation and Cytoprotective Parameters in Rat Lung

Autores
Alvarez, Silvina Monica; Biaggio, Veronica Silvina; Ciminari, Maria E.; Piguillem, Silvana N.; Pacheco Guiñazú, Anahi Belén; Escobar Correas, Sophia Melanie; Perez Chaca, Maria Valeria; Gomez, Nidia N.; Alvarez Olmedo, Daiana Gisela; Fanelli, Mariel Andrea
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim was to study the effect of androgen deprivation, focusing on oxidative stress, and cytoprotective markers, in order to better understand the possible causes of pulmonary diseases in elder people. Adult male Wistar rats were divided into three groups: control (Co), a group subjected to castration by simple orchidectomy (Ca) and a group subjected to orchidectomy that received testosterone (100 μg/kg body weight) daily from day 25 to day 30 after castration (Ca + T). Bronchoalveolar lavages (BAL) and lungs were obtained. We observed a decrease in body weight in the castrated groups. Protein content in BAL was increased in both castrated groups while nitrites did not show differences among the groups. TBARS, Catalase (CAT) and Glutathione Peroxidase (GPx) activities were significantly increased in the castrated group, returning to the control values after the administration of testosterone. AR expression was increased in the castrated groups. Nrf-2 factor was increased in both castrated groups, while NADPH oxidase (NOX) and GPx expression increased in the Ca group but showed a decrease to control values in the Ca+T group. Superoxide Dismutase (SOD) did not show differences among the treatments. The immunohistochemistry showed a decreased expression of Hsp27 and increased expression of Hsp70 The absence of androgens induces oxidative stress in lung, together with changes in the expression of cytoprotective markers. This would lead to weak lung stroma, susceptible to undergo several pulmonary diseases.
Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Biaggio, Veronica Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ciminari, Maria E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Piguillem, Silvana N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Pacheco Guiñazú, Anahi Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Escobar Correas, Sophia Melanie. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Perez Chaca, Maria Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Gomez, Nidia N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Alvarez Olmedo, Daiana Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Fanelli, Mariel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
CYTOPROTECTIVE MECHANISMS
CASTRATION
LUNG
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6987
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6987
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Androgen Deprivation and Cytoprotective Parameters in Rat LungAlvarez, Silvina MonicaBiaggio, Veronica SilvinaCiminari, Maria E.Piguillem, Silvana N.Pacheco Guiñazú, Anahi BelénEscobar Correas, Sophia MelaniePerez Chaca, Maria ValeriaGomez, Nidia N.Alvarez Olmedo, Daiana GiselaFanelli, Mariel AndreaCYTOPROTECTIVE MECHANISMSCASTRATIONLUNGhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim was to study the effect of androgen deprivation, focusing on oxidative stress, and cytoprotective markers, in order to better understand the possible causes of pulmonary diseases in elder people. Adult male Wistar rats were divided into three groups: control (Co), a group subjected to castration by simple orchidectomy (Ca) and a group subjected to orchidectomy that received testosterone (100 μg/kg body weight) daily from day 25 to day 30 after castration (Ca + T). Bronchoalveolar lavages (BAL) and lungs were obtained. We observed a decrease in body weight in the castrated groups. Protein content in BAL was increased in both castrated groups while nitrites did not show differences among the groups. TBARS, Catalase (CAT) and Glutathione Peroxidase (GPx) activities were significantly increased in the castrated group, returning to the control values after the administration of testosterone. AR expression was increased in the castrated groups. Nrf-2 factor was increased in both castrated groups, while NADPH oxidase (NOX) and GPx expression increased in the Ca group but showed a decrease to control values in the Ca+T group. Superoxide Dismutase (SOD) did not show differences among the treatments. The immunohistochemistry showed a decreased expression of Hsp27 and increased expression of Hsp70 The absence of androgens induces oxidative stress in lung, together with changes in the expression of cytoprotective markers. This would lead to weak lung stroma, susceptible to undergo several pulmonary diseases.Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Biaggio, Veronica Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Ciminari, Maria E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Piguillem, Silvana N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Pacheco Guiñazú, Anahi Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Escobar Correas, Sophia Melanie. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Perez Chaca, Maria Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Gomez, Nidia N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Alvarez Olmedo, Daiana Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Fanelli, Mariel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaARC Journals2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6987Alvarez, Silvina Monica; Biaggio, Veronica Silvina; Ciminari, Maria E.; Piguillem, Silvana N.; Pacheco Guiñazú, Anahi Belén; et al.; Androgen Deprivation and Cytoprotective Parameters in Rat Lung; ARC Journals; International Journal of Research Studies in Biosciences; 3; 1; 1-2015; 150-1632349-0357enginfo:eu-repo/semantics/altIdentifier/url/https://www.arcjournals.org/pdfs/ijrsb/v3-i1/18.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:14Zoai:ri.conicet.gov.ar:11336/6987instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:14.834CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Androgen Deprivation and Cytoprotective Parameters in Rat Lung
title Androgen Deprivation and Cytoprotective Parameters in Rat Lung
spellingShingle Androgen Deprivation and Cytoprotective Parameters in Rat Lung
Alvarez, Silvina Monica
CYTOPROTECTIVE MECHANISMS
CASTRATION
LUNG
title_short Androgen Deprivation and Cytoprotective Parameters in Rat Lung
title_full Androgen Deprivation and Cytoprotective Parameters in Rat Lung
title_fullStr Androgen Deprivation and Cytoprotective Parameters in Rat Lung
title_full_unstemmed Androgen Deprivation and Cytoprotective Parameters in Rat Lung
title_sort Androgen Deprivation and Cytoprotective Parameters in Rat Lung
dc.creator.none.fl_str_mv Alvarez, Silvina Monica
Biaggio, Veronica Silvina
Ciminari, Maria E.
Piguillem, Silvana N.
Pacheco Guiñazú, Anahi Belén
Escobar Correas, Sophia Melanie
Perez Chaca, Maria Valeria
Gomez, Nidia N.
Alvarez Olmedo, Daiana Gisela
Fanelli, Mariel Andrea
author Alvarez, Silvina Monica
author_facet Alvarez, Silvina Monica
Biaggio, Veronica Silvina
Ciminari, Maria E.
Piguillem, Silvana N.
Pacheco Guiñazú, Anahi Belén
Escobar Correas, Sophia Melanie
Perez Chaca, Maria Valeria
Gomez, Nidia N.
Alvarez Olmedo, Daiana Gisela
Fanelli, Mariel Andrea
author_role author
author2 Biaggio, Veronica Silvina
Ciminari, Maria E.
Piguillem, Silvana N.
Pacheco Guiñazú, Anahi Belén
Escobar Correas, Sophia Melanie
Perez Chaca, Maria Valeria
Gomez, Nidia N.
Alvarez Olmedo, Daiana Gisela
Fanelli, Mariel Andrea
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CYTOPROTECTIVE MECHANISMS
CASTRATION
LUNG
topic CYTOPROTECTIVE MECHANISMS
CASTRATION
LUNG
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim was to study the effect of androgen deprivation, focusing on oxidative stress, and cytoprotective markers, in order to better understand the possible causes of pulmonary diseases in elder people. Adult male Wistar rats were divided into three groups: control (Co), a group subjected to castration by simple orchidectomy (Ca) and a group subjected to orchidectomy that received testosterone (100 μg/kg body weight) daily from day 25 to day 30 after castration (Ca + T). Bronchoalveolar lavages (BAL) and lungs were obtained. We observed a decrease in body weight in the castrated groups. Protein content in BAL was increased in both castrated groups while nitrites did not show differences among the groups. TBARS, Catalase (CAT) and Glutathione Peroxidase (GPx) activities were significantly increased in the castrated group, returning to the control values after the administration of testosterone. AR expression was increased in the castrated groups. Nrf-2 factor was increased in both castrated groups, while NADPH oxidase (NOX) and GPx expression increased in the Ca group but showed a decrease to control values in the Ca+T group. Superoxide Dismutase (SOD) did not show differences among the treatments. The immunohistochemistry showed a decreased expression of Hsp27 and increased expression of Hsp70 The absence of androgens induces oxidative stress in lung, together with changes in the expression of cytoprotective markers. This would lead to weak lung stroma, susceptible to undergo several pulmonary diseases.
Fil: Alvarez, Silvina Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Biaggio, Veronica Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Ciminari, Maria E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Piguillem, Silvana N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Pacheco Guiñazú, Anahi Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Escobar Correas, Sophia Melanie. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Perez Chaca, Maria Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Gomez, Nidia N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Alvarez Olmedo, Daiana Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Fanelli, Mariel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description The aim was to study the effect of androgen deprivation, focusing on oxidative stress, and cytoprotective markers, in order to better understand the possible causes of pulmonary diseases in elder people. Adult male Wistar rats were divided into three groups: control (Co), a group subjected to castration by simple orchidectomy (Ca) and a group subjected to orchidectomy that received testosterone (100 μg/kg body weight) daily from day 25 to day 30 after castration (Ca + T). Bronchoalveolar lavages (BAL) and lungs were obtained. We observed a decrease in body weight in the castrated groups. Protein content in BAL was increased in both castrated groups while nitrites did not show differences among the groups. TBARS, Catalase (CAT) and Glutathione Peroxidase (GPx) activities were significantly increased in the castrated group, returning to the control values after the administration of testosterone. AR expression was increased in the castrated groups. Nrf-2 factor was increased in both castrated groups, while NADPH oxidase (NOX) and GPx expression increased in the Ca group but showed a decrease to control values in the Ca+T group. Superoxide Dismutase (SOD) did not show differences among the treatments. The immunohistochemistry showed a decreased expression of Hsp27 and increased expression of Hsp70 The absence of androgens induces oxidative stress in lung, together with changes in the expression of cytoprotective markers. This would lead to weak lung stroma, susceptible to undergo several pulmonary diseases.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6987
Alvarez, Silvina Monica; Biaggio, Veronica Silvina; Ciminari, Maria E.; Piguillem, Silvana N.; Pacheco Guiñazú, Anahi Belén; et al.; Androgen Deprivation and Cytoprotective Parameters in Rat Lung; ARC Journals; International Journal of Research Studies in Biosciences; 3; 1; 1-2015; 150-163
2349-0357
url http://hdl.handle.net/11336/6987
identifier_str_mv Alvarez, Silvina Monica; Biaggio, Veronica Silvina; Ciminari, Maria E.; Piguillem, Silvana N.; Pacheco Guiñazú, Anahi Belén; et al.; Androgen Deprivation and Cytoprotective Parameters in Rat Lung; ARC Journals; International Journal of Research Studies in Biosciences; 3; 1; 1-2015; 150-163
2349-0357
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.arcjournals.org/pdfs/ijrsb/v3-i1/18.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv ARC Journals
publisher.none.fl_str_mv ARC Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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