Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
- Autores
- Azcárate Peril, María Andrea; Raya, Raul Ricardo
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs.
Nous présentons la première séquence complétée d’un plasmide cryptique de Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Le plasmide pLBB1 est composé de 6127 pb ayant un contenu en CG de 44,8 %. Aucun ADN simple brin n’a été détecté par l’expérience d’hybridation, ce qui est conforme avec la notion que pLBB1 ne se réplique pas par un mécanisme de cercle roulant. Une éventuelle région de réplication de pLBB1 a été clonée et s’est avérée fonctionnelle chez Lactobacillus johnsonii et Lactococcus lactis. Le plasmide pLBB1 a démontré une similitude significative avec le plasmide pLL1212 de Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (no . d’identification GenBank AF109691). Quatre cadres de lecture ouverts (CLOs) plus grands que 100 acides aminés ont été identifiés. ORFA ressemblait à un système de primase–hélicase putatif, et ORFB et ORFC présentaient des identités limitées avec une protéine de mobilisation et une transposase, respectivement. Des expériences de cure du plasmide n’ont pas permis de décerner des fonctions aux ORFs.
Fil: Azcárate Peril, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina - Materia
-
Cryptic Plasmid
Lactobacillus
Sequence Analysis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/54892
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Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricusAzcárate Peril, María AndreaRaya, Raul RicardoCryptic PlasmidLactobacillusSequence AnalysisThe first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs.Nous présentons la première séquence complétée d’un plasmide cryptique de Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Le plasmide pLBB1 est composé de 6127 pb ayant un contenu en CG de 44,8 %. Aucun ADN simple brin n’a été détecté par l’expérience d’hybridation, ce qui est conforme avec la notion que pLBB1 ne se réplique pas par un mécanisme de cercle roulant. Une éventuelle région de réplication de pLBB1 a été clonée et s’est avérée fonctionnelle chez Lactobacillus johnsonii et Lactococcus lactis. Le plasmide pLBB1 a démontré une similitude significative avec le plasmide pLL1212 de Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (no . d’identification GenBank AF109691). Quatre cadres de lecture ouverts (CLOs) plus grands que 100 acides aminés ont été identifiés. ORFA ressemblait à un système de primase–hélicase putatif, et ORFB et ORFC présentaient des identités limitées avec une protéine de mobilisation et une transposase, respectivement. Des expériences de cure du plasmide n’ont pas permis de décerner des fonctions aux ORFs.Fil: Azcárate Peril, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaNational Research Council Canada-NRC Research Press2002-02-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54892Azcárate Peril, María Andrea; Raya, Raul Ricardo; Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 48; 2; 21-2-2002; 105-1120008-41661480-3275CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/W01-137info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/w01-137#.W2yseM5KiUkinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:06Zoai:ri.conicet.gov.ar:11336/54892instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:07.143CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus |
title |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus |
spellingShingle |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus Azcárate Peril, María Andrea Cryptic Plasmid Lactobacillus Sequence Analysis |
title_short |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus |
title_full |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus |
title_fullStr |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus |
title_full_unstemmed |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus |
title_sort |
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus |
dc.creator.none.fl_str_mv |
Azcárate Peril, María Andrea Raya, Raul Ricardo |
author |
Azcárate Peril, María Andrea |
author_facet |
Azcárate Peril, María Andrea Raya, Raul Ricardo |
author_role |
author |
author2 |
Raya, Raul Ricardo |
author2_role |
author |
dc.subject.none.fl_str_mv |
Cryptic Plasmid Lactobacillus Sequence Analysis |
topic |
Cryptic Plasmid Lactobacillus Sequence Analysis |
dc.description.none.fl_txt_mv |
The first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs. Nous présentons la première séquence complétée d’un plasmide cryptique de Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Le plasmide pLBB1 est composé de 6127 pb ayant un contenu en CG de 44,8 %. Aucun ADN simple brin n’a été détecté par l’expérience d’hybridation, ce qui est conforme avec la notion que pLBB1 ne se réplique pas par un mécanisme de cercle roulant. Une éventuelle région de réplication de pLBB1 a été clonée et s’est avérée fonctionnelle chez Lactobacillus johnsonii et Lactococcus lactis. Le plasmide pLBB1 a démontré une similitude significative avec le plasmide pLL1212 de Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (no . d’identification GenBank AF109691). Quatre cadres de lecture ouverts (CLOs) plus grands que 100 acides aminés ont été identifiés. ORFA ressemblait à un système de primase–hélicase putatif, et ORFB et ORFC présentaient des identités limitées avec une protéine de mobilisation et une transposase, respectivement. Des expériences de cure du plasmide n’ont pas permis de décerner des fonctions aux ORFs. Fil: Azcárate Peril, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina |
description |
The first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-02-21 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/54892 Azcárate Peril, María Andrea; Raya, Raul Ricardo; Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 48; 2; 21-2-2002; 105-112 0008-4166 1480-3275 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/54892 |
identifier_str_mv |
Azcárate Peril, María Andrea; Raya, Raul Ricardo; Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 48; 2; 21-2-2002; 105-112 0008-4166 1480-3275 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1139/W01-137 info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/w01-137#.W2yseM5KiUk |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
National Research Council Canada-NRC Research Press |
publisher.none.fl_str_mv |
National Research Council Canada-NRC Research Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |