Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus

Autores
Azcárate Peril, María Andrea; Raya, Raul Ricardo
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs.
Nous présentons la première séquence complétée d’un plasmide cryptique de Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Le plasmide pLBB1 est composé de 6127 pb ayant un contenu en CG de 44,8 %. Aucun ADN simple brin n’a été détecté par l’expérience d’hybridation, ce qui est conforme avec la notion que pLBB1 ne se réplique pas par un mécanisme de cercle roulant. Une éventuelle région de réplication de pLBB1 a été clonée et s’est avérée fonctionnelle chez Lactobacillus johnsonii et Lactococcus lactis. Le plasmide pLBB1 a démontré une similitude significative avec le plasmide pLL1212 de Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (no . d’identification GenBank AF109691). Quatre cadres de lecture ouverts (CLOs) plus grands que 100 acides aminés ont été identifiés. ORFA ressemblait à un système de primase–hélicase putatif, et ORFB et ORFC présentaient des identités limitées avec une protéine de mobilisation et une transposase, respectivement. Des expériences de cure du plasmide n’ont pas permis de décerner des fonctions aux ORFs.
Fil: Azcárate Peril, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Cryptic Plasmid
Lactobacillus
Sequence Analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54892

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spelling Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricusAzcárate Peril, María AndreaRaya, Raul RicardoCryptic PlasmidLactobacillusSequence AnalysisThe first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs.Nous présentons la première séquence complétée d’un plasmide cryptique de Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Le plasmide pLBB1 est composé de 6127 pb ayant un contenu en CG de 44,8 %. Aucun ADN simple brin n’a été détecté par l’expérience d’hybridation, ce qui est conforme avec la notion que pLBB1 ne se réplique pas par un mécanisme de cercle roulant. Une éventuelle région de réplication de pLBB1 a été clonée et s’est avérée fonctionnelle chez Lactobacillus johnsonii et Lactococcus lactis. Le plasmide pLBB1 a démontré une similitude significative avec le plasmide pLL1212 de Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (no . d’identification GenBank AF109691). Quatre cadres de lecture ouverts (CLOs) plus grands que 100 acides aminés ont été identifiés. ORFA ressemblait à un système de primase–hélicase putatif, et ORFB et ORFC présentaient des identités limitées avec une protéine de mobilisation et une transposase, respectivement. Des expériences de cure du plasmide n’ont pas permis de décerner des fonctions aux ORFs.Fil: Azcárate Peril, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaNational Research Council Canada-NRC Research Press2002-02-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54892Azcárate Peril, María Andrea; Raya, Raul Ricardo; Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 48; 2; 21-2-2002; 105-1120008-41661480-3275CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/W01-137info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/w01-137#.W2yseM5KiUkinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:06Zoai:ri.conicet.gov.ar:11336/54892instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:07.143CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
title Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
spellingShingle Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
Azcárate Peril, María Andrea
Cryptic Plasmid
Lactobacillus
Sequence Analysis
title_short Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
title_full Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
title_fullStr Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
title_full_unstemmed Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
title_sort Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus
dc.creator.none.fl_str_mv Azcárate Peril, María Andrea
Raya, Raul Ricardo
author Azcárate Peril, María Andrea
author_facet Azcárate Peril, María Andrea
Raya, Raul Ricardo
author_role author
author2 Raya, Raul Ricardo
author2_role author
dc.subject.none.fl_str_mv Cryptic Plasmid
Lactobacillus
Sequence Analysis
topic Cryptic Plasmid
Lactobacillus
Sequence Analysis
dc.description.none.fl_txt_mv The first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs.
Nous présentons la première séquence complétée d’un plasmide cryptique de Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Le plasmide pLBB1 est composé de 6127 pb ayant un contenu en CG de 44,8 %. Aucun ADN simple brin n’a été détecté par l’expérience d’hybridation, ce qui est conforme avec la notion que pLBB1 ne se réplique pas par un mécanisme de cercle roulant. Une éventuelle région de réplication de pLBB1 a été clonée et s’est avérée fonctionnelle chez Lactobacillus johnsonii et Lactococcus lactis. Le plasmide pLBB1 a démontré une similitude significative avec le plasmide pLL1212 de Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (no . d’identification GenBank AF109691). Quatre cadres de lecture ouverts (CLOs) plus grands que 100 acides aminés ont été identifiés. ORFA ressemblait à un système de primase–hélicase putatif, et ORFB et ORFC présentaient des identités limitées avec une protéine de mobilisation et une transposase, respectivement. Des expériences de cure du plasmide n’ont pas permis de décerner des fonctions aux ORFs.
Fil: Azcárate Peril, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description The first report of the complete nucleotide sequence of a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a GC content of 44.8%. No ssDNA was detected by hybridization experiments, which is consistent with the notion that pLBB1 does not replicate by a rolling circle mechanism. A putative replication region of pLBB1 was cloned and found to be functional in Lactobacillus johnsonii and Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp. lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691). Four open reading frames (ORFs) larger than 100 amino acids were identified. ORFA shared similarity with a putative primase-helicase system, and ORFB and ORFC exhibited limited identity with a mobilization protein and a transposase, respectively. Curing experiments did not allowed us to assign a function to the ORFs.
publishDate 2002
dc.date.none.fl_str_mv 2002-02-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54892
Azcárate Peril, María Andrea; Raya, Raul Ricardo; Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 48; 2; 21-2-2002; 105-112
0008-4166
1480-3275
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54892
identifier_str_mv Azcárate Peril, María Andrea; Raya, Raul Ricardo; Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 48; 2; 21-2-2002; 105-112
0008-4166
1480-3275
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1139/W01-137
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/w01-137#.W2yseM5KiUk
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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