Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
- Autores
- Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M.
Fil: Gutiérrez, Alejandro. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Gutiérrez, Silvia. Universidad de Guanajuato; México
Fil: García, Guadalupe. Universidad de Guanajuato; México
Fil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina - Materia
-
Modified Electrodes
Carbon Nanotubes
Polyethylenimine
8-Hydroxy 2'-Deoxiguanosine - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
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- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/66208
Ver los metadatos del registro completo
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Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in PolyethylenimineGutiérrez, AlejandroGutiérrez, SilviaGarcía, GuadalupeGalicia, LauraRivas, Gustavo AdolfoModified ElectrodesCarbon NanotubesPolyethylenimine8-Hydroxy 2'-Deoxiguanosinehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M.Fil: Gutiérrez, Alejandro. Universidad Autónoma Metropolitana Iztapalapa; MéxicoFil: Gutiérrez, Silvia. Universidad de Guanajuato; MéxicoFil: García, Guadalupe. Universidad de Guanajuato; MéxicoFil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; MéxicoFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaWiley VCH Verlag2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66208Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo; Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine; Wiley VCH Verlag; Electroanalysis; 23; 5; 5-2011; 1221-12281040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.201000677info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.201000677info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:09Zoai:ri.conicet.gov.ar:11336/66208instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:09.542CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine |
| title |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine |
| spellingShingle |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine Gutiérrez, Alejandro Modified Electrodes Carbon Nanotubes Polyethylenimine 8-Hydroxy 2'-Deoxiguanosine |
| title_short |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine |
| title_full |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine |
| title_fullStr |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine |
| title_full_unstemmed |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine |
| title_sort |
Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine |
| dc.creator.none.fl_str_mv |
Gutiérrez, Alejandro Gutiérrez, Silvia García, Guadalupe Galicia, Laura Rivas, Gustavo Adolfo |
| author |
Gutiérrez, Alejandro |
| author_facet |
Gutiérrez, Alejandro Gutiérrez, Silvia García, Guadalupe Galicia, Laura Rivas, Gustavo Adolfo |
| author_role |
author |
| author2 |
Gutiérrez, Silvia García, Guadalupe Galicia, Laura Rivas, Gustavo Adolfo |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Modified Electrodes Carbon Nanotubes Polyethylenimine 8-Hydroxy 2'-Deoxiguanosine |
| topic |
Modified Electrodes Carbon Nanotubes Polyethylenimine 8-Hydroxy 2'-Deoxiguanosine |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M. Fil: Gutiérrez, Alejandro. Universidad Autónoma Metropolitana Iztapalapa; México Fil: Gutiérrez, Silvia. Universidad de Guanajuato; México Fil: García, Guadalupe. Universidad de Guanajuato; México Fil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; México Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina |
| description |
Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-05 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/66208 Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo; Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine; Wiley VCH Verlag; Electroanalysis; 23; 5; 5-2011; 1221-1228 1040-0397 CONICET Digital CONICET |
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http://hdl.handle.net/11336/66208 |
| identifier_str_mv |
Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo; Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine; Wiley VCH Verlag; Electroanalysis; 23; 5; 5-2011; 1221-1228 1040-0397 CONICET Digital CONICET |
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eng |
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eng |
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Wiley VCH Verlag |
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Wiley VCH Verlag |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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