Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine

Autores
Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M.
Fil: Gutiérrez, Alejandro. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Gutiérrez, Silvia. Universidad de Guanajuato; México
Fil: García, Guadalupe. Universidad de Guanajuato; México
Fil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Modified Electrodes
Carbon Nanotubes
Polyethylenimine
8-Hydroxy 2'-Deoxiguanosine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66208
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in PolyethylenimineGutiérrez, AlejandroGutiérrez, SilviaGarcía, GuadalupeGalicia, LauraRivas, Gustavo AdolfoModified ElectrodesCarbon NanotubesPolyethylenimine8-Hydroxy 2'-Deoxiguanosinehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M.Fil: Gutiérrez, Alejandro. Universidad Autónoma Metropolitana Iztapalapa; MéxicoFil: Gutiérrez, Silvia. Universidad de Guanajuato; MéxicoFil: García, Guadalupe. Universidad de Guanajuato; MéxicoFil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; MéxicoFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaWiley VCH Verlag2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66208Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo; Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine; Wiley VCH Verlag; Electroanalysis; 23; 5; 5-2011; 1221-12281040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.201000677info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.201000677info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:47Zoai:ri.conicet.gov.ar:11336/66208instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:48.218CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
title Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
spellingShingle Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
Gutiérrez, Alejandro
Modified Electrodes
Carbon Nanotubes
Polyethylenimine
8-Hydroxy 2'-Deoxiguanosine
title_short Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
title_full Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
title_fullStr Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
title_full_unstemmed Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
title_sort Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
dc.creator.none.fl_str_mv Gutiérrez, Alejandro
Gutiérrez, Silvia
García, Guadalupe
Galicia, Laura
Rivas, Gustavo Adolfo
author Gutiérrez, Alejandro
author_facet Gutiérrez, Alejandro
Gutiérrez, Silvia
García, Guadalupe
Galicia, Laura
Rivas, Gustavo Adolfo
author_role author
author2 Gutiérrez, Silvia
García, Guadalupe
Galicia, Laura
Rivas, Gustavo Adolfo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Modified Electrodes
Carbon Nanotubes
Polyethylenimine
8-Hydroxy 2'-Deoxiguanosine
topic Modified Electrodes
Carbon Nanotubes
Polyethylenimine
8-Hydroxy 2'-Deoxiguanosine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M.
Fil: Gutiérrez, Alejandro. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Gutiérrez, Silvia. Universidad de Guanajuato; México
Fil: García, Guadalupe. Universidad de Guanajuato; México
Fil: Galicia, Laura. Universidad Autónoma Metropolitana Iztapalapa; México
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66208
Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo; Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine; Wiley VCH Verlag; Electroanalysis; 23; 5; 5-2011; 1221-1228
1040-0397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66208
identifier_str_mv Gutiérrez, Alejandro; Gutiérrez, Silvia; García, Guadalupe; Galicia, Laura; Rivas, Gustavo Adolfo; Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine; Wiley VCH Verlag; Electroanalysis; 23; 5; 5-2011; 1221-1228
1040-0397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.201000677
info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.201000677
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614292611006464
score 13.070432

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