Phospholipase D pathway modulates key signaling events in activated T cells

Autores
Mateos, Melina Valeria; Barreira, Maria; Ojeda, Virginia; Bustelo, Xosè R.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The T cell receptor (TCR) triggers several intracellular signaling events that are crucial for proper T cell development and function. The aim of the present work was to study the participation of the phospholipase D (PLD) pathway in signaling events elicited by the TCR stimulation in Jurkat T cells, namely: protein kinase D 1 (PKD1), extracellular signal-regulated kinase (ERK1/2) and p21- activated kinase 1 (PAK1). To suppress phosphatidic acid (PA) and diacylglycerol (DAG) generation by the PLD pathway, cells were preincubated for 1 h with 0.4% n-butanol (since in the presence of primary alcohols PLD generates phosphatidylalcohols which cannot be further dephosphorylated to DAG) and the TCR was activated with anti-CD3 antibodies. Western blot assays showed that n-butanol treatment reduced TCR-induced PKD and PAK1 activation while ERK1/2 activation was not affected. Moreover, pull down assays showed that n-butanol also reduced Rac1 activation after 10 min of stimulation with anti-CD3. Previous reports evidenced that in activated T cells PKD phosphorylates histone deacetylase 7 (HDAC7) and induces its nuclear export allowing gene expression. In agreement with the inhibition of PKD, our results showed that n-butanol also restrained the nuclear export of EGFP-HDAC7 in activated Jurkat T cells. Thus, the PLD pathway modulates key signaling events elicited by the TCR engagement
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barreira, Maria. Universidad de Salamanca; España
Fil: Ojeda, Virginia. Universidad de Salamanca; España
Fil: Bustelo, Xosè R.. Universidad de Salamanca; España
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular «Molecular mechanisms in cell signaling and gene expression»
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
PLD
T CELL
PKD
HDAC-7
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247477
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/247477
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phospholipase D pathway modulates key signaling events in activated T cellsMateos, Melina ValeriaBarreira, MariaOjeda, VirginiaBustelo, Xosè R.PLDT CELLPKDHDAC-7https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The T cell receptor (TCR) triggers several intracellular signaling events that are crucial for proper T cell development and function. The aim of the present work was to study the participation of the phospholipase D (PLD) pathway in signaling events elicited by the TCR stimulation in Jurkat T cells, namely: protein kinase D 1 (PKD1), extracellular signal-regulated kinase (ERK1/2) and p21- activated kinase 1 (PAK1). To suppress phosphatidic acid (PA) and diacylglycerol (DAG) generation by the PLD pathway, cells were preincubated for 1 h with 0.4% n-butanol (since in the presence of primary alcohols PLD generates phosphatidylalcohols which cannot be further dephosphorylated to DAG) and the TCR was activated with anti-CD3 antibodies. Western blot assays showed that n-butanol treatment reduced TCR-induced PKD and PAK1 activation while ERK1/2 activation was not affected. Moreover, pull down assays showed that n-butanol also reduced Rac1 activation after 10 min of stimulation with anti-CD3. Previous reports evidenced that in activated T cells PKD phosphorylates histone deacetylase 7 (HDAC7) and induces its nuclear export allowing gene expression. In agreement with the inhibition of PKD, our results showed that n-butanol also restrained the nuclear export of EGFP-HDAC7 in activated Jurkat T cells. Thus, the PLD pathway modulates key signaling events elicited by the TCR engagementFil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Barreira, Maria. Universidad de Salamanca; EspañaFil: Ojeda, Virginia. Universidad de Salamanca; EspañaFil: Bustelo, Xosè R.. Universidad de Salamanca; EspañaSociedad Argentina de Investigación en Bioquímica y Biología Molecular «Molecular mechanisms in cell signaling and gene expression»Buenos AiresArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología “Dr. Mario H. Burgos”2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247477Phospholipase D pathway modulates key signaling events in activated T cells; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular «Molecular mechanisms in cell signaling and gene expression»; Buenos Aires; Argentina; 2013; 47-470327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:04Zoai:ri.conicet.gov.ar:11336/247477instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:04.646CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phospholipase D pathway modulates key signaling events in activated T cells
title Phospholipase D pathway modulates key signaling events in activated T cells
spellingShingle Phospholipase D pathway modulates key signaling events in activated T cells
Mateos, Melina Valeria
PLD
T CELL
PKD
HDAC-7
title_short Phospholipase D pathway modulates key signaling events in activated T cells
title_full Phospholipase D pathway modulates key signaling events in activated T cells
title_fullStr Phospholipase D pathway modulates key signaling events in activated T cells
title_full_unstemmed Phospholipase D pathway modulates key signaling events in activated T cells
title_sort Phospholipase D pathway modulates key signaling events in activated T cells
dc.creator.none.fl_str_mv Mateos, Melina Valeria
Barreira, Maria
Ojeda, Virginia
Bustelo, Xosè R.
author Mateos, Melina Valeria
author_facet Mateos, Melina Valeria
Barreira, Maria
Ojeda, Virginia
Bustelo, Xosè R.
author_role author
author2 Barreira, Maria
Ojeda, Virginia
Bustelo, Xosè R.
author2_role author
author
author
dc.subject.none.fl_str_mv PLD
T CELL
PKD
HDAC-7
topic PLD
T CELL
PKD
HDAC-7
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The T cell receptor (TCR) triggers several intracellular signaling events that are crucial for proper T cell development and function. The aim of the present work was to study the participation of the phospholipase D (PLD) pathway in signaling events elicited by the TCR stimulation in Jurkat T cells, namely: protein kinase D 1 (PKD1), extracellular signal-regulated kinase (ERK1/2) and p21- activated kinase 1 (PAK1). To suppress phosphatidic acid (PA) and diacylglycerol (DAG) generation by the PLD pathway, cells were preincubated for 1 h with 0.4% n-butanol (since in the presence of primary alcohols PLD generates phosphatidylalcohols which cannot be further dephosphorylated to DAG) and the TCR was activated with anti-CD3 antibodies. Western blot assays showed that n-butanol treatment reduced TCR-induced PKD and PAK1 activation while ERK1/2 activation was not affected. Moreover, pull down assays showed that n-butanol also reduced Rac1 activation after 10 min of stimulation with anti-CD3. Previous reports evidenced that in activated T cells PKD phosphorylates histone deacetylase 7 (HDAC7) and induces its nuclear export allowing gene expression. In agreement with the inhibition of PKD, our results showed that n-butanol also restrained the nuclear export of EGFP-HDAC7 in activated Jurkat T cells. Thus, the PLD pathway modulates key signaling events elicited by the TCR engagement
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barreira, Maria. Universidad de Salamanca; España
Fil: Ojeda, Virginia. Universidad de Salamanca; España
Fil: Bustelo, Xosè R.. Universidad de Salamanca; España
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular «Molecular mechanisms in cell signaling and gene expression»
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description The T cell receptor (TCR) triggers several intracellular signaling events that are crucial for proper T cell development and function. The aim of the present work was to study the participation of the phospholipase D (PLD) pathway in signaling events elicited by the TCR stimulation in Jurkat T cells, namely: protein kinase D 1 (PKD1), extracellular signal-regulated kinase (ERK1/2) and p21- activated kinase 1 (PAK1). To suppress phosphatidic acid (PA) and diacylglycerol (DAG) generation by the PLD pathway, cells were preincubated for 1 h with 0.4% n-butanol (since in the presence of primary alcohols PLD generates phosphatidylalcohols which cannot be further dephosphorylated to DAG) and the TCR was activated with anti-CD3 antibodies. Western blot assays showed that n-butanol treatment reduced TCR-induced PKD and PAK1 activation while ERK1/2 activation was not affected. Moreover, pull down assays showed that n-butanol also reduced Rac1 activation after 10 min of stimulation with anti-CD3. Previous reports evidenced that in activated T cells PKD phosphorylates histone deacetylase 7 (HDAC7) and induces its nuclear export allowing gene expression. In agreement with the inhibition of PKD, our results showed that n-butanol also restrained the nuclear export of EGFP-HDAC7 in activated Jurkat T cells. Thus, the PLD pathway modulates key signaling events elicited by the TCR engagement
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247477
Phospholipase D pathway modulates key signaling events in activated T cells; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular «Molecular mechanisms in cell signaling and gene expression»; Buenos Aires; Argentina; 2013; 47-47
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247477
identifier_str_mv Phospholipase D pathway modulates key signaling events in activated T cells; Sociedad Argentina de Investigación en Bioquímica y Biología Molecular «Molecular mechanisms in cell signaling and gene expression»; Buenos Aires; Argentina; 2013; 47-47
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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