Severe haemophilia A patients have reduced numbers of peripheral memory B cells

Autores
Irigoyen, María Belén; Felippo, Marta Elena; Primiani, L.; Candela, M.; Bianco, R. P.; de Elizalde, Maria Marta; Galassi, Nora Virginia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of inhibitors is a complication of replacement treatment in Haemophilia. Loss of FVIII-specific memory B cells in the spleen is associated to down regulation of antibodies in mice treated with high doses of FVIII, but changes in B cell memory have not been described in haemophilic patients.  The aim of this study was to evaluate the phenotype of circulating lymphocytes in Severe Haemophilia A. Twenty patients with inhibitors (PI), 22 without inhibitors (P), 9 patients during Immune Tolerance Induction (ITI) treatment and 20 healthy donors (HD) were included. Peripheral blood lymphocytes were  examined by flow cytometry. Anti-FVIII antibodies were measured by Bethesda and flow cytometry. Percentages of T subsets and B lymphocytes were similar in all groups. But memory B cells (CD27+) were decreased in PI and P compared to HD but the level of significance was higher in PI (p=0.001) than P (p=0.01). PI with high level of anti-FVIII antibodies presented the lowest B memory values. CD70 expression was also lowest in PI. Non-switched CD27+ subpopulation (IgD+) was prevalent in PI but did not show statistical significance. When ITI failed, the percentages of CD27+ B cells after 12 months of ITI were lowest. In a longitudinal study performed in 4 patients, an increased percentage of CD27+ and CD70+ B cells during ITI was found. This work suggests that different peripheral lymphocyte markers as CD27 an CD70 on B cells, may be helpful to evaluate anti-FVIII response and to monitor the success of ITI.
Fil: Irigoyen, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Felippo, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Primiani, L.. Fundación Argentina de Hemofilia; Argentina
Fil: Candela, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Bianco, R. P.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: de Elizalde, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Galassi, Nora Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
anti-FVIII antibodies
Haemophilia
memory B cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271757
Acceder
id CONICETDig_9edf647279e94eaa729fdaea6e85d8f7
oai_identifier_str oai:ri.conicet.gov.ar:11336/271757
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Severe haemophilia A patients have reduced numbers of peripheral memory B cellsIrigoyen, María BelénFelippo, Marta ElenaPrimiani, L.Candela, M.Bianco, R. P.de Elizalde, Maria MartaGalassi, Nora Virginiaanti-FVIII antibodiesHaemophiliamemory B cellshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The development of inhibitors is a complication of replacement treatment in Haemophilia. Loss of FVIII-specific memory B cells in the spleen is associated to down regulation of antibodies in mice treated with high doses of FVIII, but changes in B cell memory have not been described in haemophilic patients.  The aim of this study was to evaluate the phenotype of circulating lymphocytes in Severe Haemophilia A. Twenty patients with inhibitors (PI), 22 without inhibitors (P), 9 patients during Immune Tolerance Induction (ITI) treatment and 20 healthy donors (HD) were included. Peripheral blood lymphocytes were  examined by flow cytometry. Anti-FVIII antibodies were measured by Bethesda and flow cytometry. Percentages of T subsets and B lymphocytes were similar in all groups. But memory B cells (CD27+) were decreased in PI and P compared to HD but the level of significance was higher in PI (p=0.001) than P (p=0.01). PI with high level of anti-FVIII antibodies presented the lowest B memory values. CD70 expression was also lowest in PI. Non-switched CD27+ subpopulation (IgD+) was prevalent in PI but did not show statistical significance. When ITI failed, the percentages of CD27+ B cells after 12 months of ITI were lowest. In a longitudinal study performed in 4 patients, an increased percentage of CD27+ and CD70+ B cells during ITI was found. This work suggests that different peripheral lymphocyte markers as CD27 an CD70 on B cells, may be helpful to evaluate anti-FVIII response and to monitor the success of ITI.Fil: Irigoyen, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Felippo, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Primiani, L.. Fundación Argentina de Hemofilia; ArgentinaFil: Candela, M.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Bianco, R. P.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: de Elizalde, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Galassi, Nora Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaWiley Blackwell Publishing, Inc2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271757Irigoyen, María Belén; Felippo, Marta Elena; Primiani, L.; Candela, M.; Bianco, R. P.; et al.; Severe haemophilia A patients have reduced numbers of peripheral memory B cells; Wiley Blackwell Publishing, Inc; Haemophilia The Official Journal Of The World Federation Of Hemophilia; 18; 3; 9-2011; 437-4431351-8216CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2516.2011.02642.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2516.2011.02642.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:53Zoai:ri.conicet.gov.ar:11336/271757instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:53.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Severe haemophilia A patients have reduced numbers of peripheral memory B cells
title Severe haemophilia A patients have reduced numbers of peripheral memory B cells
spellingShingle Severe haemophilia A patients have reduced numbers of peripheral memory B cells
Irigoyen, María Belén
anti-FVIII antibodies
Haemophilia
memory B cells
title_short Severe haemophilia A patients have reduced numbers of peripheral memory B cells
title_full Severe haemophilia A patients have reduced numbers of peripheral memory B cells
title_fullStr Severe haemophilia A patients have reduced numbers of peripheral memory B cells
title_full_unstemmed Severe haemophilia A patients have reduced numbers of peripheral memory B cells
title_sort Severe haemophilia A patients have reduced numbers of peripheral memory B cells
dc.creator.none.fl_str_mv Irigoyen, María Belén
Felippo, Marta Elena
Primiani, L.
Candela, M.
Bianco, R. P.
de Elizalde, Maria Marta
Galassi, Nora Virginia
author Irigoyen, María Belén
author_facet Irigoyen, María Belén
Felippo, Marta Elena
Primiani, L.
Candela, M.
Bianco, R. P.
de Elizalde, Maria Marta
Galassi, Nora Virginia
author_role author
author2 Felippo, Marta Elena
Primiani, L.
Candela, M.
Bianco, R. P.
de Elizalde, Maria Marta
Galassi, Nora Virginia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv anti-FVIII antibodies
Haemophilia
memory B cells
topic anti-FVIII antibodies
Haemophilia
memory B cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The development of inhibitors is a complication of replacement treatment in Haemophilia. Loss of FVIII-specific memory B cells in the spleen is associated to down regulation of antibodies in mice treated with high doses of FVIII, but changes in B cell memory have not been described in haemophilic patients.  The aim of this study was to evaluate the phenotype of circulating lymphocytes in Severe Haemophilia A. Twenty patients with inhibitors (PI), 22 without inhibitors (P), 9 patients during Immune Tolerance Induction (ITI) treatment and 20 healthy donors (HD) were included. Peripheral blood lymphocytes were  examined by flow cytometry. Anti-FVIII antibodies were measured by Bethesda and flow cytometry. Percentages of T subsets and B lymphocytes were similar in all groups. But memory B cells (CD27+) were decreased in PI and P compared to HD but the level of significance was higher in PI (p=0.001) than P (p=0.01). PI with high level of anti-FVIII antibodies presented the lowest B memory values. CD70 expression was also lowest in PI. Non-switched CD27+ subpopulation (IgD+) was prevalent in PI but did not show statistical significance. When ITI failed, the percentages of CD27+ B cells after 12 months of ITI were lowest. In a longitudinal study performed in 4 patients, an increased percentage of CD27+ and CD70+ B cells during ITI was found. This work suggests that different peripheral lymphocyte markers as CD27 an CD70 on B cells, may be helpful to evaluate anti-FVIII response and to monitor the success of ITI.
Fil: Irigoyen, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Felippo, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Primiani, L.. Fundación Argentina de Hemofilia; Argentina
Fil: Candela, M.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Bianco, R. P.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: de Elizalde, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Galassi, Nora Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description The development of inhibitors is a complication of replacement treatment in Haemophilia. Loss of FVIII-specific memory B cells in the spleen is associated to down regulation of antibodies in mice treated with high doses of FVIII, but changes in B cell memory have not been described in haemophilic patients.  The aim of this study was to evaluate the phenotype of circulating lymphocytes in Severe Haemophilia A. Twenty patients with inhibitors (PI), 22 without inhibitors (P), 9 patients during Immune Tolerance Induction (ITI) treatment and 20 healthy donors (HD) were included. Peripheral blood lymphocytes were  examined by flow cytometry. Anti-FVIII antibodies were measured by Bethesda and flow cytometry. Percentages of T subsets and B lymphocytes were similar in all groups. But memory B cells (CD27+) were decreased in PI and P compared to HD but the level of significance was higher in PI (p=0.001) than P (p=0.01). PI with high level of anti-FVIII antibodies presented the lowest B memory values. CD70 expression was also lowest in PI. Non-switched CD27+ subpopulation (IgD+) was prevalent in PI but did not show statistical significance. When ITI failed, the percentages of CD27+ B cells after 12 months of ITI were lowest. In a longitudinal study performed in 4 patients, an increased percentage of CD27+ and CD70+ B cells during ITI was found. This work suggests that different peripheral lymphocyte markers as CD27 an CD70 on B cells, may be helpful to evaluate anti-FVIII response and to monitor the success of ITI.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271757
Irigoyen, María Belén; Felippo, Marta Elena; Primiani, L.; Candela, M.; Bianco, R. P.; et al.; Severe haemophilia A patients have reduced numbers of peripheral memory B cells; Wiley Blackwell Publishing, Inc; Haemophilia The Official Journal Of The World Federation Of Hemophilia; 18; 3; 9-2011; 437-443
1351-8216
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271757
identifier_str_mv Irigoyen, María Belén; Felippo, Marta Elena; Primiani, L.; Candela, M.; Bianco, R. P.; et al.; Severe haemophilia A patients have reduced numbers of peripheral memory B cells; Wiley Blackwell Publishing, Inc; Haemophilia The Official Journal Of The World Federation Of Hemophilia; 18; 3; 9-2011; 437-443
1351-8216
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2516.2011.02642.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2516.2011.02642.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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