The role of pH fronts in reversible electroporation

• Autores: Turjanski, P.; Olaiz, N.; Maglietti, F.; Michinski, S.; Suárez, C.; Molina, F.V.; Marshall, G.
• Año de publicación: 2011
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: PLoS ONE 2011;6(4)
• Materia: acidification; article; comparative study; controlled study; electrode; electrogenetherapy; electroporation; electrostimulation therapy; gene therapy; ion transport; pH; reversible electroporation; tissue necrosis; Animals; Electrochemistry; Electrodes; Electroporation; Genetic Techniques; Genetic Therapy; Humans; Hydrogen-Ion Concentration; Models, Theoretical; Necrosis; Plasmids; Time Factors
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

We present experimental measurements and theoretical predictions of ion transport in agar gels during reversible electroporation (ECT) for conditions typical to many clinical studies found in the literature, revealing the presence of pH fronts emerging from both electrodes. These results suggest that pH fronts are immediate and substantial. Since they might give rise to tissue necrosis, an unwanted condition in clinical applications of ECT as well as in irreversible electroporation (IRE) and in electrogenetherapy (EGT), it is important to quantify their extent and evolution. Here, a tracking technique is used to follow the space-time evolution of these pH fronts. It is found that they scale in time as t1/2 characteristic of a predominantly diffusive process. Comparing ECT pH fronts with those arising in electrotherapy (EChT), another treatment applying constant electric fields whose main goal is tissue necrosis, a striking result is observed: anodic acidification is larger in ECT than in EChT, suggesting that tissue necrosis could also be greater. Ways to minimize these adverse effects in ECT are suggested. © 2011 Turjanski et al.
Fil:Turjanski, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Suárez, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Molina, F.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.