Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations

Autores
Ruiz, María Sol; Sánchez, María Belén; Vera Contreras, Yuly Masiel; Agrielo, Evangelina; Alonso, Marta; Altuna, María Eugenia; Anchordoqui, María Sol; Asinari, Mariana; Bonetto, María Elisa; Camargo, Mauricio; Giere, Isabel; González, Javier; Granda Alacote, Ana Cecilia; Guerra, Javier; Gutiérrez, Marina; Maldonado, Cecilia; Makiya, Ricard; Manrique, Gonzalo; Monaco, María Eugenia; Rozo, Juan Carlos; Santamaría Martín, Carlos Jose; Seravalle, Analía; Zea, Olga; Zubillaga, María Noel; Mordoh, José; Larripa, Irene Beatriz; Bianchini, Michele
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The quantitation of BCR-ABL1 mRNA is mandatory for chronic myeloid leukemia (CML) patients, and RT-qPCR is the most extensively used method in testing laboratories worldwide. Nevertheless, substantial variation in RT-qPCR results makes inter-laboratory comparability hard. To facilitate inter-laboratory comparative assessment, an international scale (IS) for BCR-ABL1 was proposed.The laboratory-specific conversion factor (CF) to the IS can be derived from the World Health Organization (WHO) genetic reference panel; however, this material is limited to the manufacturers to produce and calibrate secondary reference reagents. Therefore, we developed secondary reference calibrators, as lyophilized cellular material, aligned to the IS. Our purpose was both to re-evaluate the CF in 18 previously harmonized laboratories and to propagate the IS to new laboratories.Results Our field trial including 30 laboratories across Latin America showed that, after correction of raw BCR-ABL1/ABL1 ratios using CF, the relative mean bias was significantly reduced. We also performed a follow-up of participating laboratories by annually revalidating the process; our results support the need for continuous revalidation of CFs. All participating laboratories also received a calibrator to determine the limit of quantification (LOQ); 90% of them could reproducibly detect BCR-ABL1, indicating that these laboratories can report a consistent deep molecular response. In addition, aiming to investigate the variability of BCR-ABL1 measurements across different RNA inputs, we calculated PCR efficiency for each individual assay by using different amounts of RNA.Conclusions In conclusion, for the first time in Latin America, we have successfully organized a harmonization platform for BCR-ABL1 measurement that could be of immediate clinical benefit for monitoring the molecular response of patients in low-resource regions.
Fil: Ruiz, María Sol. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sánchez, María Belén. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
Fil: Vera Contreras, Yuly Masiel. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
Fil: Agrielo, Evangelina. Laboratorio Especialidades Bioquímicas; Argentina
Fil: Alonso, Marta. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Altuna, María Eugenia. Clinica Roberto Raña; Argentina
Fil: Anchordoqui, María Sol. Argenomics; Argentina
Fil: Asinari, Mariana. Hospital Privado Centro Médico de Córdoba; Argentina
Fil: Bonetto, María Elisa. Hospital Rawson San Juan; Argentina
Fil: Camargo, Mauricio. Laboratorio Medellin; Colombia
Fil: Giere, Isabel. Fundación Para Combatir la Leucemia; Argentina
Fil: González, Javier. Meyer Lab; Paraguay
Fil: Granda Alacote, Ana Cecilia. Laboratorios Medicos Lima; Perú
Fil: Guerra, Javier. Nanopharmacia Diagnóstica Mexico; México
Fil: Gutiérrez, Marina. Stamboulian; Argentina
Fil: Maldonado, Cecilia. Manlab; Argentina
Fil: Makiya, Ricard. No especifíca;
Fil: Manrique, Gonzalo. Asociacion Española de Montevideo; Uruguay
Fil: Monaco, María Eugenia. Laboratorio Tucuman; Argentina
Fil: Rozo, Juan Carlos. Unidad de Diagnóstico Hemato Oncológico; Colombia
Fil: Santamaría Martín, Carlos Jose. Hospital de Niños San Jose de Costa Rica; Costa Rica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Seravalle, Analía. Civic Rosario; Argentina
Fil: Zea, Olga. Laboratorio Medellin Colombia; Colombia
Fil: Zubillaga, María Noel. Asociación Española de Socorros Mutuos; Uruguay
Fil: Mordoh, José. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
Fil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bianchini, Michele. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
LMC
BCR-ABL1
Harmonization
International Scale
RT-QPCR
PH-IS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146466

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oai_identifier_str oai:ri.conicet.gov.ar:11336/146466
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendationsRuiz, María SolSánchez, María BelénVera Contreras, Yuly MasielAgrielo, EvangelinaAlonso, MartaAltuna, María EugeniaAnchordoqui, María SolAsinari, MarianaBonetto, María ElisaCamargo, MauricioGiere, IsabelGonzález, JavierGranda Alacote, Ana CeciliaGuerra, JavierGutiérrez, MarinaMaldonado, CeciliaMakiya, RicardManrique, GonzaloMonaco, María EugeniaRozo, Juan CarlosSantamaría Martín, Carlos JoseSeravalle, AnalíaZea, OlgaZubillaga, María NoelMordoh, JoséLarripa, Irene BeatrizBianchini, MicheleLMCBCR-ABL1HarmonizationInternational ScaleRT-QPCRPH-IShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The quantitation of BCR-ABL1 mRNA is mandatory for chronic myeloid leukemia (CML) patients, and RT-qPCR is the most extensively used method in testing laboratories worldwide. Nevertheless, substantial variation in RT-qPCR results makes inter-laboratory comparability hard. To facilitate inter-laboratory comparative assessment, an international scale (IS) for BCR-ABL1 was proposed.The laboratory-specific conversion factor (CF) to the IS can be derived from the World Health Organization (WHO) genetic reference panel; however, this material is limited to the manufacturers to produce and calibrate secondary reference reagents. Therefore, we developed secondary reference calibrators, as lyophilized cellular material, aligned to the IS. Our purpose was both to re-evaluate the CF in 18 previously harmonized laboratories and to propagate the IS to new laboratories.Results Our field trial including 30 laboratories across Latin America showed that, after correction of raw BCR-ABL1/ABL1 ratios using CF, the relative mean bias was significantly reduced. We also performed a follow-up of participating laboratories by annually revalidating the process; our results support the need for continuous revalidation of CFs. All participating laboratories also received a calibrator to determine the limit of quantification (LOQ); 90% of them could reproducibly detect BCR-ABL1, indicating that these laboratories can report a consistent deep molecular response. In addition, aiming to investigate the variability of BCR-ABL1 measurements across different RNA inputs, we calculated PCR efficiency for each individual assay by using different amounts of RNA.Conclusions In conclusion, for the first time in Latin America, we have successfully organized a harmonization platform for BCR-ABL1 measurement that could be of immediate clinical benefit for monitoring the molecular response of patients in low-resource regions.Fil: Ruiz, María Sol. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez, María Belén. Fundación Cáncer. Centro de Investigaciones Oncológicas; ArgentinaFil: Vera Contreras, Yuly Masiel. Fundación Cáncer. Centro de Investigaciones Oncológicas; ArgentinaFil: Agrielo, Evangelina. Laboratorio Especialidades Bioquímicas; ArgentinaFil: Alonso, Marta. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Altuna, María Eugenia. Clinica Roberto Raña; ArgentinaFil: Anchordoqui, María Sol. Argenomics; ArgentinaFil: Asinari, Mariana. Hospital Privado Centro Médico de Córdoba; ArgentinaFil: Bonetto, María Elisa. Hospital Rawson San Juan; ArgentinaFil: Camargo, Mauricio. Laboratorio Medellin; ColombiaFil: Giere, Isabel. Fundación Para Combatir la Leucemia; ArgentinaFil: González, Javier. Meyer Lab; ParaguayFil: Granda Alacote, Ana Cecilia. Laboratorios Medicos Lima; PerúFil: Guerra, Javier. Nanopharmacia Diagnóstica Mexico; MéxicoFil: Gutiérrez, Marina. Stamboulian; ArgentinaFil: Maldonado, Cecilia. Manlab; ArgentinaFil: Makiya, Ricard. No especifíca;Fil: Manrique, Gonzalo. Asociacion Española de Montevideo; UruguayFil: Monaco, María Eugenia. Laboratorio Tucuman; ArgentinaFil: Rozo, Juan Carlos. Unidad de Diagnóstico Hemato Oncológico; ColombiaFil: Santamaría Martín, Carlos Jose. Hospital de Niños San Jose de Costa Rica; Costa Rica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Seravalle, Analía. Civic Rosario; ArgentinaFil: Zea, Olga. Laboratorio Medellin Colombia; ColombiaFil: Zubillaga, María Noel. Asociación Española de Socorros Mutuos; UruguayFil: Mordoh, José. Fundación Cáncer. Centro de Investigaciones Oncológicas; ArgentinaFil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Bianchini, Michele. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaDe Gruyter2020-05-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146466Ruiz, María Sol; Sánchez, María Belén; Vera Contreras, Yuly Masiel; Agrielo, Evangelina; Alonso, Marta; et al.; Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations; De Gruyter; Clinical Chemistry and Laboratory Medicine; 58; 12; 11-5-2020; 2025-20351434-6621CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/journals/cclm/ahead-of-print/article-10.1515-cclm-2019-1283/article-10.1515-cclm-2019-1283.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1515/cclm-2019-1283info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:47Zoai:ri.conicet.gov.ar:11336/146466instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:47.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
title Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
spellingShingle Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
Ruiz, María Sol
LMC
BCR-ABL1
Harmonization
International Scale
RT-QPCR
PH-IS
title_short Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
title_full Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
title_fullStr Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
title_full_unstemmed Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
title_sort Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations
dc.creator.none.fl_str_mv Ruiz, María Sol
Sánchez, María Belén
Vera Contreras, Yuly Masiel
Agrielo, Evangelina
Alonso, Marta
Altuna, María Eugenia
Anchordoqui, María Sol
Asinari, Mariana
Bonetto, María Elisa
Camargo, Mauricio
Giere, Isabel
González, Javier
Granda Alacote, Ana Cecilia
Guerra, Javier
Gutiérrez, Marina
Maldonado, Cecilia
Makiya, Ricard
Manrique, Gonzalo
Monaco, María Eugenia
Rozo, Juan Carlos
Santamaría Martín, Carlos Jose
Seravalle, Analía
Zea, Olga
Zubillaga, María Noel
Mordoh, José
Larripa, Irene Beatriz
Bianchini, Michele
author Ruiz, María Sol
author_facet Ruiz, María Sol
Sánchez, María Belén
Vera Contreras, Yuly Masiel
Agrielo, Evangelina
Alonso, Marta
Altuna, María Eugenia
Anchordoqui, María Sol
Asinari, Mariana
Bonetto, María Elisa
Camargo, Mauricio
Giere, Isabel
González, Javier
Granda Alacote, Ana Cecilia
Guerra, Javier
Gutiérrez, Marina
Maldonado, Cecilia
Makiya, Ricard
Manrique, Gonzalo
Monaco, María Eugenia
Rozo, Juan Carlos
Santamaría Martín, Carlos Jose
Seravalle, Analía
Zea, Olga
Zubillaga, María Noel
Mordoh, José
Larripa, Irene Beatriz
Bianchini, Michele
author_role author
author2 Sánchez, María Belén
Vera Contreras, Yuly Masiel
Agrielo, Evangelina
Alonso, Marta
Altuna, María Eugenia
Anchordoqui, María Sol
Asinari, Mariana
Bonetto, María Elisa
Camargo, Mauricio
Giere, Isabel
González, Javier
Granda Alacote, Ana Cecilia
Guerra, Javier
Gutiérrez, Marina
Maldonado, Cecilia
Makiya, Ricard
Manrique, Gonzalo
Monaco, María Eugenia
Rozo, Juan Carlos
Santamaría Martín, Carlos Jose
Seravalle, Analía
Zea, Olga
Zubillaga, María Noel
Mordoh, José
Larripa, Irene Beatriz
Bianchini, Michele
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LMC
BCR-ABL1
Harmonization
International Scale
RT-QPCR
PH-IS
topic LMC
BCR-ABL1
Harmonization
International Scale
RT-QPCR
PH-IS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The quantitation of BCR-ABL1 mRNA is mandatory for chronic myeloid leukemia (CML) patients, and RT-qPCR is the most extensively used method in testing laboratories worldwide. Nevertheless, substantial variation in RT-qPCR results makes inter-laboratory comparability hard. To facilitate inter-laboratory comparative assessment, an international scale (IS) for BCR-ABL1 was proposed.The laboratory-specific conversion factor (CF) to the IS can be derived from the World Health Organization (WHO) genetic reference panel; however, this material is limited to the manufacturers to produce and calibrate secondary reference reagents. Therefore, we developed secondary reference calibrators, as lyophilized cellular material, aligned to the IS. Our purpose was both to re-evaluate the CF in 18 previously harmonized laboratories and to propagate the IS to new laboratories.Results Our field trial including 30 laboratories across Latin America showed that, after correction of raw BCR-ABL1/ABL1 ratios using CF, the relative mean bias was significantly reduced. We also performed a follow-up of participating laboratories by annually revalidating the process; our results support the need for continuous revalidation of CFs. All participating laboratories also received a calibrator to determine the limit of quantification (LOQ); 90% of them could reproducibly detect BCR-ABL1, indicating that these laboratories can report a consistent deep molecular response. In addition, aiming to investigate the variability of BCR-ABL1 measurements across different RNA inputs, we calculated PCR efficiency for each individual assay by using different amounts of RNA.Conclusions In conclusion, for the first time in Latin America, we have successfully organized a harmonization platform for BCR-ABL1 measurement that could be of immediate clinical benefit for monitoring the molecular response of patients in low-resource regions.
Fil: Ruiz, María Sol. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sánchez, María Belén. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
Fil: Vera Contreras, Yuly Masiel. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
Fil: Agrielo, Evangelina. Laboratorio Especialidades Bioquímicas; Argentina
Fil: Alonso, Marta. Hospital Nacional Profesor Alejandro Posadas; Argentina
Fil: Altuna, María Eugenia. Clinica Roberto Raña; Argentina
Fil: Anchordoqui, María Sol. Argenomics; Argentina
Fil: Asinari, Mariana. Hospital Privado Centro Médico de Córdoba; Argentina
Fil: Bonetto, María Elisa. Hospital Rawson San Juan; Argentina
Fil: Camargo, Mauricio. Laboratorio Medellin; Colombia
Fil: Giere, Isabel. Fundación Para Combatir la Leucemia; Argentina
Fil: González, Javier. Meyer Lab; Paraguay
Fil: Granda Alacote, Ana Cecilia. Laboratorios Medicos Lima; Perú
Fil: Guerra, Javier. Nanopharmacia Diagnóstica Mexico; México
Fil: Gutiérrez, Marina. Stamboulian; Argentina
Fil: Maldonado, Cecilia. Manlab; Argentina
Fil: Makiya, Ricard. No especifíca;
Fil: Manrique, Gonzalo. Asociacion Española de Montevideo; Uruguay
Fil: Monaco, María Eugenia. Laboratorio Tucuman; Argentina
Fil: Rozo, Juan Carlos. Unidad de Diagnóstico Hemato Oncológico; Colombia
Fil: Santamaría Martín, Carlos Jose. Hospital de Niños San Jose de Costa Rica; Costa Rica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Seravalle, Analía. Civic Rosario; Argentina
Fil: Zea, Olga. Laboratorio Medellin Colombia; Colombia
Fil: Zubillaga, María Noel. Asociación Española de Socorros Mutuos; Uruguay
Fil: Mordoh, José. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina
Fil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Bianchini, Michele. Fundación Cáncer. Centro de Investigaciones Oncológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The quantitation of BCR-ABL1 mRNA is mandatory for chronic myeloid leukemia (CML) patients, and RT-qPCR is the most extensively used method in testing laboratories worldwide. Nevertheless, substantial variation in RT-qPCR results makes inter-laboratory comparability hard. To facilitate inter-laboratory comparative assessment, an international scale (IS) for BCR-ABL1 was proposed.The laboratory-specific conversion factor (CF) to the IS can be derived from the World Health Organization (WHO) genetic reference panel; however, this material is limited to the manufacturers to produce and calibrate secondary reference reagents. Therefore, we developed secondary reference calibrators, as lyophilized cellular material, aligned to the IS. Our purpose was both to re-evaluate the CF in 18 previously harmonized laboratories and to propagate the IS to new laboratories.Results Our field trial including 30 laboratories across Latin America showed that, after correction of raw BCR-ABL1/ABL1 ratios using CF, the relative mean bias was significantly reduced. We also performed a follow-up of participating laboratories by annually revalidating the process; our results support the need for continuous revalidation of CFs. All participating laboratories also received a calibrator to determine the limit of quantification (LOQ); 90% of them could reproducibly detect BCR-ABL1, indicating that these laboratories can report a consistent deep molecular response. In addition, aiming to investigate the variability of BCR-ABL1 measurements across different RNA inputs, we calculated PCR efficiency for each individual assay by using different amounts of RNA.Conclusions In conclusion, for the first time in Latin America, we have successfully organized a harmonization platform for BCR-ABL1 measurement that could be of immediate clinical benefit for monitoring the molecular response of patients in low-resource regions.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146466
Ruiz, María Sol; Sánchez, María Belén; Vera Contreras, Yuly Masiel; Agrielo, Evangelina; Alonso, Marta; et al.; Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations; De Gruyter; Clinical Chemistry and Laboratory Medicine; 58; 12; 11-5-2020; 2025-2035
1434-6621
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146466
identifier_str_mv Ruiz, María Sol; Sánchez, María Belén; Vera Contreras, Yuly Masiel; Agrielo, Evangelina; Alonso, Marta; et al.; Programme for Harmonization to the International Scale in Latin America for BCR-ABL1 quantification in CML patients: Findings and recommendations; De Gruyter; Clinical Chemistry and Laboratory Medicine; 58; 12; 11-5-2020; 2025-2035
1434-6621
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1515/cclm-2019-1283
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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