UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes

Autores
Soni, Awakash; Klebanov Akopyan, Olga; Erben, Esteban Daniel; Plaschkes, Inbar; Benyamini, Hadar; Mitesser, Vera; Harel, Amnon; Yamin, Katereena; Onn, Itay; Shlomai, Joseph
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.
Fil: Soni, Awakash. The Hebrew University of Jerusalem; Israel
Fil: Klebanov Akopyan, Olga. The Hebrew University of Jerusalem; Israel
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Plaschkes, Inbar. The Hebrew University of Jerusalem; Israel
Fil: Benyamini, Hadar. The Hebrew University of Jerusalem; Israel
Fil: Mitesser, Vera. The Hebrew University of Jerusalem; Israel
Fil: Harel, Amnon. Bar-Ilan University; Israel
Fil: Yamin, Katereena. Bar-Ilan University; Israel
Fil: Onn, Itay. Bar-Ilan University; Israel
Fil: Shlomai, Joseph. The Hebrew University of Jerusalem; Israel
Materia
TRYPANOSOMA BRUCEI
UMSBP2
CHROMATIN
ANTIGENIC VARIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229068
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/229068
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomesSoni, AwakashKlebanov Akopyan, OlgaErben, Esteban DanielPlaschkes, InbarBenyamini, HadarMitesser, VeraHarel, AmnonYamin, KatereenaOnn, ItayShlomai, JosephTRYPANOSOMA BRUCEIUMSBP2CHROMATINANTIGENIC VARIATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.Fil: Soni, Awakash. The Hebrew University of Jerusalem; IsraelFil: Klebanov Akopyan, Olga. The Hebrew University of Jerusalem; IsraelFil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; AlemaniaFil: Plaschkes, Inbar. The Hebrew University of Jerusalem; IsraelFil: Benyamini, Hadar. The Hebrew University of Jerusalem; IsraelFil: Mitesser, Vera. The Hebrew University of Jerusalem; IsraelFil: Harel, Amnon. Bar-Ilan University; IsraelFil: Yamin, Katereena. Bar-Ilan University; IsraelFil: Onn, Itay. Bar-Ilan University; IsraelFil: Shlomai, Joseph. The Hebrew University of Jerusalem; IsraelOxford University Press2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229068Soni, Awakash; Klebanov Akopyan, Olga; Erben, Esteban Daniel; Plaschkes, Inbar; Benyamini, Hadar; et al.; UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes; Oxford University Press; Nucleic Acids Research; 51; 11; 5-2023; 5678-56980305-10481362-4962CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad402/7173837info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkad402info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:24Zoai:ri.conicet.gov.ar:11336/229068instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:24.479CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
title UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
spellingShingle UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
Soni, Awakash
TRYPANOSOMA BRUCEI
UMSBP2
CHROMATIN
ANTIGENIC VARIATION
title_short UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
title_full UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
title_fullStr UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
title_full_unstemmed UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
title_sort UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes
dc.creator.none.fl_str_mv Soni, Awakash
Klebanov Akopyan, Olga
Erben, Esteban Daniel
Plaschkes, Inbar
Benyamini, Hadar
Mitesser, Vera
Harel, Amnon
Yamin, Katereena
Onn, Itay
Shlomai, Joseph
author Soni, Awakash
author_facet Soni, Awakash
Klebanov Akopyan, Olga
Erben, Esteban Daniel
Plaschkes, Inbar
Benyamini, Hadar
Mitesser, Vera
Harel, Amnon
Yamin, Katereena
Onn, Itay
Shlomai, Joseph
author_role author
author2 Klebanov Akopyan, Olga
Erben, Esteban Daniel
Plaschkes, Inbar
Benyamini, Hadar
Mitesser, Vera
Harel, Amnon
Yamin, Katereena
Onn, Itay
Shlomai, Joseph
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA BRUCEI
UMSBP2
CHROMATIN
ANTIGENIC VARIATION
topic TRYPANOSOMA BRUCEI
UMSBP2
CHROMATIN
ANTIGENIC VARIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.
Fil: Soni, Awakash. The Hebrew University of Jerusalem; Israel
Fil: Klebanov Akopyan, Olga. The Hebrew University of Jerusalem; Israel
Fil: Erben, Esteban Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina. Ruprecht Karls Universitat Heidelberg; Alemania
Fil: Plaschkes, Inbar. The Hebrew University of Jerusalem; Israel
Fil: Benyamini, Hadar. The Hebrew University of Jerusalem; Israel
Fil: Mitesser, Vera. The Hebrew University of Jerusalem; Israel
Fil: Harel, Amnon. Bar-Ilan University; Israel
Fil: Yamin, Katereena. Bar-Ilan University; Israel
Fil: Onn, Itay. Bar-Ilan University; Israel
Fil: Shlomai, Joseph. The Hebrew University of Jerusalem; Israel
description Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229068
Soni, Awakash; Klebanov Akopyan, Olga; Erben, Esteban Daniel; Plaschkes, Inbar; Benyamini, Hadar; et al.; UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes; Oxford University Press; Nucleic Acids Research; 51; 11; 5-2023; 5678-5698
0305-1048
1362-4962
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229068
identifier_str_mv Soni, Awakash; Klebanov Akopyan, Olga; Erben, Esteban Daniel; Plaschkes, Inbar; Benyamini, Hadar; et al.; UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes; Oxford University Press; Nucleic Acids Research; 51; 11; 5-2023; 5678-5698
0305-1048
1362-4962
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad402/7173837
info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkad402
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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