In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates

Autores
Dominguez, Facundo Gabriel; Vereschuk, Manuela Lizz; Zapata, Pedro Dario; Alvarenga, Adriana Elizabet
Año de publicación
2022
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
White thread blight is a disease caused by a fungal complex that causes drying of leaves, stems and branches in Yerba mateand tea plants and causes serious losses in the yield of these crops. Conventional synthetic fungicides are largely consideredas the most effective and cost-efficient means for disease management. One of the most used broad-spectrum fungicides forthe control of foliar diseases is copper sulphate pentahydrate. Sensitivity of the pathogens to copper varies greatly, dependingon the product and the fungus. However, to date, no published studies are available on the inhibition/tolerance to coppersulphate concentrations of the white thread blight fungal isolates. This research investigated the grow inhibition of sevenisolates (ACK2, AFE1, ASD4, AKD2, ACJ2, ACB1 and APC1) associated with white thread blight disease by the poisonedfood method. Czapek agar medium was supplemented with copper sulphate pentahydrate (CuSO4.5H2O) at concentrations of100ppm, 500ppm, 1000ppm and 5000ppm. Twenty milliliters of each sterile medium were dispensed into Petri dishes andinoculated with a 5mm disc cut from the periphery of a 7 days-old culture. Each isolate was inoculated onto two plates andincubated at 28°C. Mycelial growth of the isolates was determined by linear measurements of colony diameters with anelectronic caliper at four intervals. To determine fungicide or fungistatic effect the discs which concentrations that completelyinhibited growth were inoculated in Potato Dextrose Agar (PDA) medium. Three isolates (AKD2, ACB1, AFE1) showed amaximum inhibition of mycelial growth at 500ppm. Additionally, two isolates (ACK2, ACJ2) were inhibited at a concentrationof 1000 ppm. Maximum effect of inhibition of growth was observed at the highest concentration at 5000 ppm. Infungicide/fungistatic assay we verified that at 1000 ppm copper sulphate acts as a fungistatic, inhibiting the development ofthe fungus but without causing its total elimination. These results suggest that white thread blight pathogens are sensitive tocopper sulphate-based fungicides.
Fil: Dominguez, Facundo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Vereschuk, Manuela Lizz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Alvarenga, Adriana Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
LVII Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XVI Reunión Anual de Sociedad Argentina de Microbiología General
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
Fungicides
Growth inhibition
White thread blight disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210602
Acceder
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network_name_str CONICET Digital (CONICET)
spelling In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolatesDominguez, Facundo GabrielVereschuk, Manuela LizzZapata, Pedro DarioAlvarenga, Adriana ElizabetFungicidesGrowth inhibitionWhite thread blight diseasehttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4White thread blight is a disease caused by a fungal complex that causes drying of leaves, stems and branches in Yerba mateand tea plants and causes serious losses in the yield of these crops. Conventional synthetic fungicides are largely consideredas the most effective and cost-efficient means for disease management. One of the most used broad-spectrum fungicides forthe control of foliar diseases is copper sulphate pentahydrate. Sensitivity of the pathogens to copper varies greatly, dependingon the product and the fungus. However, to date, no published studies are available on the inhibition/tolerance to coppersulphate concentrations of the white thread blight fungal isolates. This research investigated the grow inhibition of sevenisolates (ACK2, AFE1, ASD4, AKD2, ACJ2, ACB1 and APC1) associated with white thread blight disease by the poisonedfood method. Czapek agar medium was supplemented with copper sulphate pentahydrate (CuSO4.5H2O) at concentrations of100ppm, 500ppm, 1000ppm and 5000ppm. Twenty milliliters of each sterile medium were dispensed into Petri dishes andinoculated with a 5mm disc cut from the periphery of a 7 days-old culture. Each isolate was inoculated onto two plates andincubated at 28°C. Mycelial growth of the isolates was determined by linear measurements of colony diameters with anelectronic caliper at four intervals. To determine fungicide or fungistatic effect the discs which concentrations that completelyinhibited growth were inoculated in Potato Dextrose Agar (PDA) medium. Three isolates (AKD2, ACB1, AFE1) showed amaximum inhibition of mycelial growth at 500ppm. Additionally, two isolates (ACK2, ACJ2) were inhibited at a concentrationof 1000 ppm. Maximum effect of inhibition of growth was observed at the highest concentration at 5000 ppm. Infungicide/fungistatic assay we verified that at 1000 ppm copper sulphate acts as a fungistatic, inhibiting the development ofthe fungus but without causing its total elimination. These results suggest that white thread blight pathogens are sensitive tocopper sulphate-based fungicides.Fil: Dominguez, Facundo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Vereschuk, Manuela Lizz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Alvarenga, Adriana Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaLVII Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XVI Reunión Anual de Sociedad Argentina de Microbiología GeneralRosarioArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralSociedad Argentina de Investigaciones en Bioquímica y Biología Molecular2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210602In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates; LVII Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XVI Reunión Anual de Sociedad Argentina de Microbiología General; Rosario; Argentina; 2021; 99-99CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:26Zoai:ri.conicet.gov.ar:11336/210602instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:26.465CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
title In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
spellingShingle In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
Dominguez, Facundo Gabriel
Fungicides
Growth inhibition
White thread blight disease
title_short In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
title_full In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
title_fullStr In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
title_full_unstemmed In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
title_sort In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates
dc.creator.none.fl_str_mv Dominguez, Facundo Gabriel
Vereschuk, Manuela Lizz
Zapata, Pedro Dario
Alvarenga, Adriana Elizabet
author Dominguez, Facundo Gabriel
author_facet Dominguez, Facundo Gabriel
Vereschuk, Manuela Lizz
Zapata, Pedro Dario
Alvarenga, Adriana Elizabet
author_role author
author2 Vereschuk, Manuela Lizz
Zapata, Pedro Dario
Alvarenga, Adriana Elizabet
author2_role author
author
author
dc.subject.none.fl_str_mv Fungicides
Growth inhibition
White thread blight disease
topic Fungicides
Growth inhibition
White thread blight disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv White thread blight is a disease caused by a fungal complex that causes drying of leaves, stems and branches in Yerba mateand tea plants and causes serious losses in the yield of these crops. Conventional synthetic fungicides are largely consideredas the most effective and cost-efficient means for disease management. One of the most used broad-spectrum fungicides forthe control of foliar diseases is copper sulphate pentahydrate. Sensitivity of the pathogens to copper varies greatly, dependingon the product and the fungus. However, to date, no published studies are available on the inhibition/tolerance to coppersulphate concentrations of the white thread blight fungal isolates. This research investigated the grow inhibition of sevenisolates (ACK2, AFE1, ASD4, AKD2, ACJ2, ACB1 and APC1) associated with white thread blight disease by the poisonedfood method. Czapek agar medium was supplemented with copper sulphate pentahydrate (CuSO4.5H2O) at concentrations of100ppm, 500ppm, 1000ppm and 5000ppm. Twenty milliliters of each sterile medium were dispensed into Petri dishes andinoculated with a 5mm disc cut from the periphery of a 7 days-old culture. Each isolate was inoculated onto two plates andincubated at 28°C. Mycelial growth of the isolates was determined by linear measurements of colony diameters with anelectronic caliper at four intervals. To determine fungicide or fungistatic effect the discs which concentrations that completelyinhibited growth were inoculated in Potato Dextrose Agar (PDA) medium. Three isolates (AKD2, ACB1, AFE1) showed amaximum inhibition of mycelial growth at 500ppm. Additionally, two isolates (ACK2, ACJ2) were inhibited at a concentrationof 1000 ppm. Maximum effect of inhibition of growth was observed at the highest concentration at 5000 ppm. Infungicide/fungistatic assay we verified that at 1000 ppm copper sulphate acts as a fungistatic, inhibiting the development ofthe fungus but without causing its total elimination. These results suggest that white thread blight pathogens are sensitive tocopper sulphate-based fungicides.
Fil: Dominguez, Facundo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Vereschuk, Manuela Lizz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Alvarenga, Adriana Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
LVII Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XVI Reunión Anual de Sociedad Argentina de Microbiología General
Rosario
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description White thread blight is a disease caused by a fungal complex that causes drying of leaves, stems and branches in Yerba mateand tea plants and causes serious losses in the yield of these crops. Conventional synthetic fungicides are largely consideredas the most effective and cost-efficient means for disease management. One of the most used broad-spectrum fungicides forthe control of foliar diseases is copper sulphate pentahydrate. Sensitivity of the pathogens to copper varies greatly, dependingon the product and the fungus. However, to date, no published studies are available on the inhibition/tolerance to coppersulphate concentrations of the white thread blight fungal isolates. This research investigated the grow inhibition of sevenisolates (ACK2, AFE1, ASD4, AKD2, ACJ2, ACB1 and APC1) associated with white thread blight disease by the poisonedfood method. Czapek agar medium was supplemented with copper sulphate pentahydrate (CuSO4.5H2O) at concentrations of100ppm, 500ppm, 1000ppm and 5000ppm. Twenty milliliters of each sterile medium were dispensed into Petri dishes andinoculated with a 5mm disc cut from the periphery of a 7 days-old culture. Each isolate was inoculated onto two plates andincubated at 28°C. Mycelial growth of the isolates was determined by linear measurements of colony diameters with anelectronic caliper at four intervals. To determine fungicide or fungistatic effect the discs which concentrations that completelyinhibited growth were inoculated in Potato Dextrose Agar (PDA) medium. Three isolates (AKD2, ACB1, AFE1) showed amaximum inhibition of mycelial growth at 500ppm. Additionally, two isolates (ACK2, ACJ2) were inhibited at a concentrationof 1000 ppm. Maximum effect of inhibition of growth was observed at the highest concentration at 5000 ppm. Infungicide/fungistatic assay we verified that at 1000 ppm copper sulphate acts as a fungistatic, inhibiting the development ofthe fungus but without causing its total elimination. These results suggest that white thread blight pathogens are sensitive tocopper sulphate-based fungicides.
publishDate 2022
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210602
In vitro inhibition assay of copper sulphate as fungicide against White Thread Blight fungal isolates; LVII Reunión Anual Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; XVI Reunión Anual de Sociedad Argentina de Microbiología General; Rosario; Argentina; 2021; 99-99
CONICET Digital
CONICET
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