Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay

Autores
Salvioni Recalde, Oscar Daniel; Rolón, Miriam Soledad; Velázquez, Myriam Celeste; Kowalewski, Miguel Martin; Ruiz Diaz, Jorge Javier Alfonso; Rojas de Arias, Antonieta; Ozório Moraes, Milton; Magdinier Gomes, Harrison; de Azevedo Baêta, Bruna; Dias Cordeiro, Matheus; Vega Gómez, María Celeste
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity of this bacterial family in ticks and hosts, both domestic and wild, as well as blood meal sources of free-living ticks in northeastern Paraguay. The bacteria were identified using PCR-HRM, a method optimized for this purpose, while the identification of ticks and their blood meal was performed using conventional PCR. All amplified products were subsequently sequenced. The bacteria detected in the blood hosts included Ehrlichia canis, Anaplasma platys, and Anaplasma phagocytophilum, Candidatus Anaplasma boleense, and Wolbachia spp., which had not been previously reported in the country. Free-living and parasitic ticks on dogs (Canis lupus familiaris) and wild armadillos (Dasypus novemcinctus) were collected and identified as Rhipicephalus sanguineus and Amblyomma spp. The species E. canis, A. platys, A. phagocytophilum, and Ca. A. boleense were detected in domestic dog ticks, and E. canis and A. platys were found for the first time in armadillos and free-living ticks. Blood feeding sources detected in free-living ticks were rodents, humans, armadillos and dogs. Results show a high diversity of tick-borne pathogens circulating among domestic and wild animals in the northeastern region of Paraguay.
Fil: Salvioni Recalde, Oscar Daniel. Centro para el Desarrollo de la Investigación Cientifica; Paraguay. Instituto Oswaldo Cruz; Brasil
Fil: Rolón, Miriam Soledad. Centro para el Desarrollo de la Investigación Cientifica; Paraguay
Fil: Velázquez, Myriam Celeste. Fundacion Moises Bertoni; Paraguay
Fil: Kowalewski, Miguel Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia". Estación Biológica de Usos Múltiples (Sede Corrientes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina
Fil: Ruiz Diaz, Jorge Javier Alfonso. Centro para el Desarrollo de la Investigación Cientifica; Paraguay
Fil: Rojas de Arias, Antonieta. Centro para el Desarrollo de la Investigación Cientifica; Paraguay
Fil: Ozório Moraes, Milton. Instituto Oswaldo Cruz; Brasil
Fil: Magdinier Gomes, Harrison. Instituto Oswaldo Cruz; Brasil
Fil: de Azevedo Baêta, Bruna. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: Dias Cordeiro, Matheus. Universidade Federal do Estado do Rio de Janeiro; Brasil
Fil: Vega Gómez, María Celeste. Centro para el Desarrollo de la Investigación Cientifica; Paraguay. Instituto Oswaldo Cruz; Brasil
Materia
FREE-LIVING TICKS
TICK-BORNE DISEASES
EHRLICHIA SPP
PARASITOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262625
Acceder
id CONICETDig_9cbf1676967f49119bda226b2654754b
oai_identifier_str oai:ri.conicet.gov.ar:11336/262625
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in ParaguaySalvioni Recalde, Oscar DanielRolón, Miriam SoledadVelázquez, Myriam CelesteKowalewski, Miguel MartinRuiz Diaz, Jorge Javier AlfonsoRojas de Arias, AntonietaOzório Moraes, MiltonMagdinier Gomes, Harrisonde Azevedo Baêta, BrunaDias Cordeiro, MatheusVega Gómez, María CelesteFREE-LIVING TICKSTICK-BORNE DISEASESEHRLICHIA SPPPARASITOLOGYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity of this bacterial family in ticks and hosts, both domestic and wild, as well as blood meal sources of free-living ticks in northeastern Paraguay. The bacteria were identified using PCR-HRM, a method optimized for this purpose, while the identification of ticks and their blood meal was performed using conventional PCR. All amplified products were subsequently sequenced. The bacteria detected in the blood hosts included Ehrlichia canis, Anaplasma platys, and Anaplasma phagocytophilum, Candidatus Anaplasma boleense, and Wolbachia spp., which had not been previously reported in the country. Free-living and parasitic ticks on dogs (Canis lupus familiaris) and wild armadillos (Dasypus novemcinctus) were collected and identified as Rhipicephalus sanguineus and Amblyomma spp. The species E. canis, A. platys, A. phagocytophilum, and Ca. A. boleense were detected in domestic dog ticks, and E. canis and A. platys were found for the first time in armadillos and free-living ticks. Blood feeding sources detected in free-living ticks were rodents, humans, armadillos and dogs. Results show a high diversity of tick-borne pathogens circulating among domestic and wild animals in the northeastern region of Paraguay.Fil: Salvioni Recalde, Oscar Daniel. Centro para el Desarrollo de la Investigación Cientifica; Paraguay. Instituto Oswaldo Cruz; BrasilFil: Rolón, Miriam Soledad. Centro para el Desarrollo de la Investigación Cientifica; ParaguayFil: Velázquez, Myriam Celeste. Fundacion Moises Bertoni; ParaguayFil: Kowalewski, Miguel Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia". Estación Biológica de Usos Múltiples (Sede Corrientes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; ArgentinaFil: Ruiz Diaz, Jorge Javier Alfonso. Centro para el Desarrollo de la Investigación Cientifica; ParaguayFil: Rojas de Arias, Antonieta. Centro para el Desarrollo de la Investigación Cientifica; ParaguayFil: Ozório Moraes, Milton. Instituto Oswaldo Cruz; BrasilFil: Magdinier Gomes, Harrison. Instituto Oswaldo Cruz; BrasilFil: de Azevedo Baêta, Bruna. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Dias Cordeiro, Matheus. Universidade Federal do Estado do Rio de Janeiro; BrasilFil: Vega Gómez, María Celeste. Centro para el Desarrollo de la Investigación Cientifica; Paraguay. Instituto Oswaldo Cruz; BrasilMultidisciplinary Digital Publishing Institute2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262625Salvioni Recalde, Oscar Daniel; Rolón, Miriam Soledad; Velázquez, Myriam Celeste; Kowalewski, Miguel Martin; Ruiz Diaz, Jorge Javier Alfonso; et al.; Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay; Multidisciplinary Digital Publishing Institute; Microorganisms; 12; 9; 9-2024; 1-182076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/12/9/1893info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12091893info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:53Zoai:ri.conicet.gov.ar:11336/262625instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:53.747CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
title Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
spellingShingle Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
Salvioni Recalde, Oscar Daniel
FREE-LIVING TICKS
TICK-BORNE DISEASES
EHRLICHIA SPP
PARASITOLOGY
title_short Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
title_full Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
title_fullStr Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
title_full_unstemmed Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
title_sort Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay
dc.creator.none.fl_str_mv Salvioni Recalde, Oscar Daniel
Rolón, Miriam Soledad
Velázquez, Myriam Celeste
Kowalewski, Miguel Martin
Ruiz Diaz, Jorge Javier Alfonso
Rojas de Arias, Antonieta
Ozório Moraes, Milton
Magdinier Gomes, Harrison
de Azevedo Baêta, Bruna
Dias Cordeiro, Matheus
Vega Gómez, María Celeste
author Salvioni Recalde, Oscar Daniel
author_facet Salvioni Recalde, Oscar Daniel
Rolón, Miriam Soledad
Velázquez, Myriam Celeste
Kowalewski, Miguel Martin
Ruiz Diaz, Jorge Javier Alfonso
Rojas de Arias, Antonieta
Ozório Moraes, Milton
Magdinier Gomes, Harrison
de Azevedo Baêta, Bruna
Dias Cordeiro, Matheus
Vega Gómez, María Celeste
author_role author
author2 Rolón, Miriam Soledad
Velázquez, Myriam Celeste
Kowalewski, Miguel Martin
Ruiz Diaz, Jorge Javier Alfonso
Rojas de Arias, Antonieta
Ozório Moraes, Milton
Magdinier Gomes, Harrison
de Azevedo Baêta, Bruna
Dias Cordeiro, Matheus
Vega Gómez, María Celeste
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FREE-LIVING TICKS
TICK-BORNE DISEASES
EHRLICHIA SPP
PARASITOLOGY
topic FREE-LIVING TICKS
TICK-BORNE DISEASES
EHRLICHIA SPP
PARASITOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity of this bacterial family in ticks and hosts, both domestic and wild, as well as blood meal sources of free-living ticks in northeastern Paraguay. The bacteria were identified using PCR-HRM, a method optimized for this purpose, while the identification of ticks and their blood meal was performed using conventional PCR. All amplified products were subsequently sequenced. The bacteria detected in the blood hosts included Ehrlichia canis, Anaplasma platys, and Anaplasma phagocytophilum, Candidatus Anaplasma boleense, and Wolbachia spp., which had not been previously reported in the country. Free-living and parasitic ticks on dogs (Canis lupus familiaris) and wild armadillos (Dasypus novemcinctus) were collected and identified as Rhipicephalus sanguineus and Amblyomma spp. The species E. canis, A. platys, A. phagocytophilum, and Ca. A. boleense were detected in domestic dog ticks, and E. canis and A. platys were found for the first time in armadillos and free-living ticks. Blood feeding sources detected in free-living ticks were rodents, humans, armadillos and dogs. Results show a high diversity of tick-borne pathogens circulating among domestic and wild animals in the northeastern region of Paraguay.
Fil: Salvioni Recalde, Oscar Daniel. Centro para el Desarrollo de la Investigación Cientifica; Paraguay. Instituto Oswaldo Cruz; Brasil
Fil: Rolón, Miriam Soledad. Centro para el Desarrollo de la Investigación Cientifica; Paraguay
Fil: Velázquez, Myriam Celeste. Fundacion Moises Bertoni; Paraguay
Fil: Kowalewski, Miguel Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia". Estación Biológica de Usos Múltiples (Sede Corrientes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina
Fil: Ruiz Diaz, Jorge Javier Alfonso. Centro para el Desarrollo de la Investigación Cientifica; Paraguay
Fil: Rojas de Arias, Antonieta. Centro para el Desarrollo de la Investigación Cientifica; Paraguay
Fil: Ozório Moraes, Milton. Instituto Oswaldo Cruz; Brasil
Fil: Magdinier Gomes, Harrison. Instituto Oswaldo Cruz; Brasil
Fil: de Azevedo Baêta, Bruna. Universidade Federal Rural do Rio de Janeiro; Brasil
Fil: Dias Cordeiro, Matheus. Universidade Federal do Estado do Rio de Janeiro; Brasil
Fil: Vega Gómez, María Celeste. Centro para el Desarrollo de la Investigación Cientifica; Paraguay. Instituto Oswaldo Cruz; Brasil
description Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity of this bacterial family in ticks and hosts, both domestic and wild, as well as blood meal sources of free-living ticks in northeastern Paraguay. The bacteria were identified using PCR-HRM, a method optimized for this purpose, while the identification of ticks and their blood meal was performed using conventional PCR. All amplified products were subsequently sequenced. The bacteria detected in the blood hosts included Ehrlichia canis, Anaplasma platys, and Anaplasma phagocytophilum, Candidatus Anaplasma boleense, and Wolbachia spp., which had not been previously reported in the country. Free-living and parasitic ticks on dogs (Canis lupus familiaris) and wild armadillos (Dasypus novemcinctus) were collected and identified as Rhipicephalus sanguineus and Amblyomma spp. The species E. canis, A. platys, A. phagocytophilum, and Ca. A. boleense were detected in domestic dog ticks, and E. canis and A. platys were found for the first time in armadillos and free-living ticks. Blood feeding sources detected in free-living ticks were rodents, humans, armadillos and dogs. Results show a high diversity of tick-borne pathogens circulating among domestic and wild animals in the northeastern region of Paraguay.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262625
Salvioni Recalde, Oscar Daniel; Rolón, Miriam Soledad; Velázquez, Myriam Celeste; Kowalewski, Miguel Martin; Ruiz Diaz, Jorge Javier Alfonso; et al.; Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay; Multidisciplinary Digital Publishing Institute; Microorganisms; 12; 9; 9-2024; 1-18
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262625
identifier_str_mv Salvioni Recalde, Oscar Daniel; Rolón, Miriam Soledad; Velázquez, Myriam Celeste; Kowalewski, Miguel Martin; Ruiz Diaz, Jorge Javier Alfonso; et al.; Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of Anaplasma phagocytophilum and Candidatus Anaplasma boleense in Paraguay; Multidisciplinary Digital Publishing Institute; Microorganisms; 12; 9; 9-2024; 1-18
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/12/9/1893
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms12091893
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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