Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails

Autores
Narváez, Cristóbal; Rios, Juan Manuel; Píriz, Gabriela; Sanchez Hernandez, Juan C.; Sabat, Pablo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Effects of pesticides on non-target organisms have been studied in several taxa at different levels of biological organization, from enzymatic to behavioral responses. Although the physiological responses may be associated with higher energy costs, little is known about metabolic costs of pesticide detoxification in birds. To fill this gap, we exposed orally (diet) 15-d old Coturnix coturnix japonica individuals to sublethal doses of chlorpyrifos (10 and 20 mg active ingredient/kg dry food) for four weeks. Carboxylesterase (CbE), butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities were periodically measured in multiple tissues along with measurements of resting (RMR) and maximum metabolic rates (Msum). Furthermore, glucuronic acid in bird excreta was also assessed at the end of the trial. While CbE and BChE activities were inhibited by chlorpyrifos in all tissues during the third and fourth weeks following pesticide treatment, AChE activity was unaffected. At this sampling times, both Msum and RMR expansibility decreased. These results suggest that the exposure to chlorpyrifos caused a negative effect on aerobic performance. Additionally, excretion rate of glucuronic acid was up to 2-fold higher in the 20-mg/kg group than in the control and 10-mg/kg chlorpyrifos groups. The inhibition of CbE and BChE activities corroborated that these enzymes are fulfilling their role as bioscavengers for organophosphate pesticides, decreasing its concentration and thus protecting AChE activity against inhibition by chlorpyrifos.
Fil: Narváez, Cristóbal. Universidad de Chile; Chile
Fil: Rios, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina
Fil: Píriz, Gabriela. Universidad de Chile; Chile
Fil: Sanchez Hernandez, Juan C.. Universidad de Castilla-La Mancha; España
Fil: Sabat, Pablo. Universidad de Chile; Chile
Materia
AEROBIC PERFORMANCE
DETOXIFICATION
ESTERASES
ORGANOPHOSPHORUS PESTICIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182643
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/182643
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quailsNarváez, CristóbalRios, Juan ManuelPíriz, GabrielaSanchez Hernandez, Juan C.Sabat, PabloAEROBIC PERFORMANCEDETOXIFICATIONESTERASESORGANOPHOSPHORUS PESTICIDEShttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Effects of pesticides on non-target organisms have been studied in several taxa at different levels of biological organization, from enzymatic to behavioral responses. Although the physiological responses may be associated with higher energy costs, little is known about metabolic costs of pesticide detoxification in birds. To fill this gap, we exposed orally (diet) 15-d old Coturnix coturnix japonica individuals to sublethal doses of chlorpyrifos (10 and 20 mg active ingredient/kg dry food) for four weeks. Carboxylesterase (CbE), butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities were periodically measured in multiple tissues along with measurements of resting (RMR) and maximum metabolic rates (Msum). Furthermore, glucuronic acid in bird excreta was also assessed at the end of the trial. While CbE and BChE activities were inhibited by chlorpyrifos in all tissues during the third and fourth weeks following pesticide treatment, AChE activity was unaffected. At this sampling times, both Msum and RMR expansibility decreased. These results suggest that the exposure to chlorpyrifos caused a negative effect on aerobic performance. Additionally, excretion rate of glucuronic acid was up to 2-fold higher in the 20-mg/kg group than in the control and 10-mg/kg chlorpyrifos groups. The inhibition of CbE and BChE activities corroborated that these enzymes are fulfilling their role as bioscavengers for organophosphate pesticides, decreasing its concentration and thus protecting AChE activity against inhibition by chlorpyrifos.Fil: Narváez, Cristóbal. Universidad de Chile; ChileFil: Rios, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; ArgentinaFil: Píriz, Gabriela. Universidad de Chile; ChileFil: Sanchez Hernandez, Juan C.. Universidad de Castilla-La Mancha; EspañaFil: Sabat, Pablo. Universidad de Chile; ChilePergamon-Elsevier Science Ltd2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182643Narváez, Cristóbal; Rios, Juan Manuel; Píriz, Gabriela; Sanchez Hernandez, Juan C.; Sabat, Pablo; Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails; Pergamon-Elsevier Science Ltd; Chemosphere; 144; 1-2016; 775-7840045-6535CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0045653515301478info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2015.09.060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:58Zoai:ri.conicet.gov.ar:11336/182643instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:58.471CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
title Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
spellingShingle Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
Narváez, Cristóbal
AEROBIC PERFORMANCE
DETOXIFICATION
ESTERASES
ORGANOPHOSPHORUS PESTICIDES
title_short Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
title_full Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
title_fullStr Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
title_full_unstemmed Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
title_sort Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails
dc.creator.none.fl_str_mv Narváez, Cristóbal
Rios, Juan Manuel
Píriz, Gabriela
Sanchez Hernandez, Juan C.
Sabat, Pablo
author Narváez, Cristóbal
author_facet Narváez, Cristóbal
Rios, Juan Manuel
Píriz, Gabriela
Sanchez Hernandez, Juan C.
Sabat, Pablo
author_role author
author2 Rios, Juan Manuel
Píriz, Gabriela
Sanchez Hernandez, Juan C.
Sabat, Pablo
author2_role author
author
author
author
dc.subject.none.fl_str_mv AEROBIC PERFORMANCE
DETOXIFICATION
ESTERASES
ORGANOPHOSPHORUS PESTICIDES
topic AEROBIC PERFORMANCE
DETOXIFICATION
ESTERASES
ORGANOPHOSPHORUS PESTICIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Effects of pesticides on non-target organisms have been studied in several taxa at different levels of biological organization, from enzymatic to behavioral responses. Although the physiological responses may be associated with higher energy costs, little is known about metabolic costs of pesticide detoxification in birds. To fill this gap, we exposed orally (diet) 15-d old Coturnix coturnix japonica individuals to sublethal doses of chlorpyrifos (10 and 20 mg active ingredient/kg dry food) for four weeks. Carboxylesterase (CbE), butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities were periodically measured in multiple tissues along with measurements of resting (RMR) and maximum metabolic rates (Msum). Furthermore, glucuronic acid in bird excreta was also assessed at the end of the trial. While CbE and BChE activities were inhibited by chlorpyrifos in all tissues during the third and fourth weeks following pesticide treatment, AChE activity was unaffected. At this sampling times, both Msum and RMR expansibility decreased. These results suggest that the exposure to chlorpyrifos caused a negative effect on aerobic performance. Additionally, excretion rate of glucuronic acid was up to 2-fold higher in the 20-mg/kg group than in the control and 10-mg/kg chlorpyrifos groups. The inhibition of CbE and BChE activities corroborated that these enzymes are fulfilling their role as bioscavengers for organophosphate pesticides, decreasing its concentration and thus protecting AChE activity against inhibition by chlorpyrifos.
Fil: Narváez, Cristóbal. Universidad de Chile; Chile
Fil: Rios, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina
Fil: Píriz, Gabriela. Universidad de Chile; Chile
Fil: Sanchez Hernandez, Juan C.. Universidad de Castilla-La Mancha; España
Fil: Sabat, Pablo. Universidad de Chile; Chile
description Effects of pesticides on non-target organisms have been studied in several taxa at different levels of biological organization, from enzymatic to behavioral responses. Although the physiological responses may be associated with higher energy costs, little is known about metabolic costs of pesticide detoxification in birds. To fill this gap, we exposed orally (diet) 15-d old Coturnix coturnix japonica individuals to sublethal doses of chlorpyrifos (10 and 20 mg active ingredient/kg dry food) for four weeks. Carboxylesterase (CbE), butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) activities were periodically measured in multiple tissues along with measurements of resting (RMR) and maximum metabolic rates (Msum). Furthermore, glucuronic acid in bird excreta was also assessed at the end of the trial. While CbE and BChE activities were inhibited by chlorpyrifos in all tissues during the third and fourth weeks following pesticide treatment, AChE activity was unaffected. At this sampling times, both Msum and RMR expansibility decreased. These results suggest that the exposure to chlorpyrifos caused a negative effect on aerobic performance. Additionally, excretion rate of glucuronic acid was up to 2-fold higher in the 20-mg/kg group than in the control and 10-mg/kg chlorpyrifos groups. The inhibition of CbE and BChE activities corroborated that these enzymes are fulfilling their role as bioscavengers for organophosphate pesticides, decreasing its concentration and thus protecting AChE activity against inhibition by chlorpyrifos.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182643
Narváez, Cristóbal; Rios, Juan Manuel; Píriz, Gabriela; Sanchez Hernandez, Juan C.; Sabat, Pablo; Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails; Pergamon-Elsevier Science Ltd; Chemosphere; 144; 1-2016; 775-784
0045-6535
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182643
identifier_str_mv Narváez, Cristóbal; Rios, Juan Manuel; Píriz, Gabriela; Sanchez Hernandez, Juan C.; Sabat, Pablo; Subchronic exposure to chlorpyrifos affects energy expenditure and detoxification capacity in juvenile Japanese quails; Pergamon-Elsevier Science Ltd; Chemosphere; 144; 1-2016; 775-784
0045-6535
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0045653515301478
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2015.09.060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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