Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis

Autores
Lascano, Cecilia Ines; Ferrari, Ana; Gauna, Lidia Esther; Cocca, Claudia Marcela; Cochón, Adriana C.; Verrengia Guerrero, Noemí Rosario; Venturino, Andres
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of the present study was to evaluate the concentration- and time-dependent effects of the organophosphorus insecticides malathion and azinphos-methyl on polyamine metabolism, and relate them to normal and altered embryonic development of the common toad Rhinella arenarum. Control embryos showed that the higher polyamines spermidine and spermine acquired importance with respect to the diamine putrescine as embryonic development progressed. The activity of ornithine decarboxylase significantly decreased in complete operculum embryos. Continuous exposure to malathion caused a decrease in polyamine levels during embryonic development. However, there was an increase in putrescine levels in complete operculum embryos exposed to a sublethal concentration of the insecticide. Embryos exposed to malathion displayed a decrease in fresh weight and size, along with an increase in the number of malformed individuals. R. arenarum embryos exposed to a lethal concentration of azinphos-methyl showed an increase in putrescine levels and a decrease in spermidine and spermine levels, accompanied by an increase in  ornithine decarboxylase activity. In conclusion, as the embryonic development of the toad R. arenarum progresses, polyamine metabolism shifts to higher polyamine levels with a more preponderant contribution of spermidine and spermine with respect to putrescine and involves a dramatic change in ornithine decarboxylase activity, one of the key regulatory enzymes of the pathway.Organophosphorus insecticides are capable of altering polyamine metabolism, slowing embryo development in parallel with a reduction in spermidine and spermine levels. An increase in the oxidative degradation of polyamines might be involved in the toxic action of organophosphorus insecticides and might also be related to other effects such as teratogenesis.
Fil: Lascano, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Ferrari, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina
Fil: Gauna, Lidia Esther. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Cocca, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Cochón, Adriana C.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Verrengia Guerrero, Noemí Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Venturino, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina
Materia
ORGANOPHOSPHORUS PESTICIDE
SPERMIDINE
PUTRESCINE
SPERMINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273226
Acceder
id CONICETDig_99e99d14832c747f83154b5f04a860fd
oai_identifier_str oai:ri.conicet.gov.ar:11336/273226
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesisLascano, Cecilia InesFerrari, AnaGauna, Lidia EstherCocca, Claudia MarcelaCochón, Adriana C.Verrengia Guerrero, Noemí RosarioVenturino, AndresORGANOPHOSPHORUS PESTICIDESPERMIDINEPUTRESCINESPERMINEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The objective of the present study was to evaluate the concentration- and time-dependent effects of the organophosphorus insecticides malathion and azinphos-methyl on polyamine metabolism, and relate them to normal and altered embryonic development of the common toad Rhinella arenarum. Control embryos showed that the higher polyamines spermidine and spermine acquired importance with respect to the diamine putrescine as embryonic development progressed. The activity of ornithine decarboxylase significantly decreased in complete operculum embryos. Continuous exposure to malathion caused a decrease in polyamine levels during embryonic development. However, there was an increase in putrescine levels in complete operculum embryos exposed to a sublethal concentration of the insecticide. Embryos exposed to malathion displayed a decrease in fresh weight and size, along with an increase in the number of malformed individuals. R. arenarum embryos exposed to a lethal concentration of azinphos-methyl showed an increase in putrescine levels and a decrease in spermidine and spermine levels, accompanied by an increase in  ornithine decarboxylase activity. In conclusion, as the embryonic development of the toad R. arenarum progresses, polyamine metabolism shifts to higher polyamine levels with a more preponderant contribution of spermidine and spermine with respect to putrescine and involves a dramatic change in ornithine decarboxylase activity, one of the key regulatory enzymes of the pathway.Organophosphorus insecticides are capable of altering polyamine metabolism, slowing embryo development in parallel with a reduction in spermidine and spermine levels. An increase in the oxidative degradation of polyamines might be involved in the toxic action of organophosphorus insecticides and might also be related to other effects such as teratogenesis.Fil: Lascano, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; ArgentinaFil: Ferrari, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; ArgentinaFil: Gauna, Lidia Esther. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; ArgentinaFil: Cocca, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; ArgentinaFil: Cochón, Adriana C.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Verrengia Guerrero, Noemí Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Venturino, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; ArgentinaAcademic Press Inc Elsevier Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273226Lascano, Cecilia Ines; Ferrari, Ana; Gauna, Lidia Esther; Cocca, Claudia Marcela; Cochón, Adriana C.; et al.; Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis; Academic Press Inc Elsevier Science; Pesticide Biochemistry And Physiology; 101; 3; 11-2011; 240-2470048-3575CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S004835751100157Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2011.10.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:38:26Zoai:ri.conicet.gov.ar:11336/273226instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:38:26.328CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
title Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
spellingShingle Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
Lascano, Cecilia Ines
ORGANOPHOSPHORUS PESTICIDE
SPERMIDINE
PUTRESCINE
SPERMINE
title_short Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
title_full Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
title_fullStr Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
title_full_unstemmed Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
title_sort Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
dc.creator.none.fl_str_mv Lascano, Cecilia Ines
Ferrari, Ana
Gauna, Lidia Esther
Cocca, Claudia Marcela
Cochón, Adriana C.
Verrengia Guerrero, Noemí Rosario
Venturino, Andres
author Lascano, Cecilia Ines
author_facet Lascano, Cecilia Ines
Ferrari, Ana
Gauna, Lidia Esther
Cocca, Claudia Marcela
Cochón, Adriana C.
Verrengia Guerrero, Noemí Rosario
Venturino, Andres
author_role author
author2 Ferrari, Ana
Gauna, Lidia Esther
Cocca, Claudia Marcela
Cochón, Adriana C.
Verrengia Guerrero, Noemí Rosario
Venturino, Andres
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ORGANOPHOSPHORUS PESTICIDE
SPERMIDINE
PUTRESCINE
SPERMINE
topic ORGANOPHOSPHORUS PESTICIDE
SPERMIDINE
PUTRESCINE
SPERMINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The objective of the present study was to evaluate the concentration- and time-dependent effects of the organophosphorus insecticides malathion and azinphos-methyl on polyamine metabolism, and relate them to normal and altered embryonic development of the common toad Rhinella arenarum. Control embryos showed that the higher polyamines spermidine and spermine acquired importance with respect to the diamine putrescine as embryonic development progressed. The activity of ornithine decarboxylase significantly decreased in complete operculum embryos. Continuous exposure to malathion caused a decrease in polyamine levels during embryonic development. However, there was an increase in putrescine levels in complete operculum embryos exposed to a sublethal concentration of the insecticide. Embryos exposed to malathion displayed a decrease in fresh weight and size, along with an increase in the number of malformed individuals. R. arenarum embryos exposed to a lethal concentration of azinphos-methyl showed an increase in putrescine levels and a decrease in spermidine and spermine levels, accompanied by an increase in  ornithine decarboxylase activity. In conclusion, as the embryonic development of the toad R. arenarum progresses, polyamine metabolism shifts to higher polyamine levels with a more preponderant contribution of spermidine and spermine with respect to putrescine and involves a dramatic change in ornithine decarboxylase activity, one of the key regulatory enzymes of the pathway.Organophosphorus insecticides are capable of altering polyamine metabolism, slowing embryo development in parallel with a reduction in spermidine and spermine levels. An increase in the oxidative degradation of polyamines might be involved in the toxic action of organophosphorus insecticides and might also be related to other effects such as teratogenesis.
Fil: Lascano, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Ferrari, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina
Fil: Gauna, Lidia Esther. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Cocca, Claudia Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Cochón, Adriana C.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Verrengia Guerrero, Noemí Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Venturino, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería. Departamento de Química. Laboratorio de Investigaciones Bioquímicas, Químicas y de Medio Ambiente; Argentina
description The objective of the present study was to evaluate the concentration- and time-dependent effects of the organophosphorus insecticides malathion and azinphos-methyl on polyamine metabolism, and relate them to normal and altered embryonic development of the common toad Rhinella arenarum. Control embryos showed that the higher polyamines spermidine and spermine acquired importance with respect to the diamine putrescine as embryonic development progressed. The activity of ornithine decarboxylase significantly decreased in complete operculum embryos. Continuous exposure to malathion caused a decrease in polyamine levels during embryonic development. However, there was an increase in putrescine levels in complete operculum embryos exposed to a sublethal concentration of the insecticide. Embryos exposed to malathion displayed a decrease in fresh weight and size, along with an increase in the number of malformed individuals. R. arenarum embryos exposed to a lethal concentration of azinphos-methyl showed an increase in putrescine levels and a decrease in spermidine and spermine levels, accompanied by an increase in  ornithine decarboxylase activity. In conclusion, as the embryonic development of the toad R. arenarum progresses, polyamine metabolism shifts to higher polyamine levels with a more preponderant contribution of spermidine and spermine with respect to putrescine and involves a dramatic change in ornithine decarboxylase activity, one of the key regulatory enzymes of the pathway.Organophosphorus insecticides are capable of altering polyamine metabolism, slowing embryo development in parallel with a reduction in spermidine and spermine levels. An increase in the oxidative degradation of polyamines might be involved in the toxic action of organophosphorus insecticides and might also be related to other effects such as teratogenesis.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273226
Lascano, Cecilia Ines; Ferrari, Ana; Gauna, Lidia Esther; Cocca, Claudia Marcela; Cochón, Adriana C.; et al.; Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis; Academic Press Inc Elsevier Science; Pesticide Biochemistry And Physiology; 101; 3; 11-2011; 240-247
0048-3575
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273226
identifier_str_mv Lascano, Cecilia Ines; Ferrari, Ana; Gauna, Lidia Esther; Cocca, Claudia Marcela; Cochón, Adriana C.; et al.; Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis; Academic Press Inc Elsevier Science; Pesticide Biochemistry And Physiology; 101; 3; 11-2011; 240-247
0048-3575
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S004835751100157X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2011.10.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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