Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations

Autores
Grosso, Carla Gisela; Blariza, María José; Mougabure Cueto, Gastón Adolfo; Picollo, Maria Ines; Garcia, Beatriz Alicia
Año de publicación
2016
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytochrome P450 monooxygenases play a predominant role in the metabolism of insecticides. Many insect P450genes have frequently been associated with detoxification processes allowing the insect to become tolerant orresistant to insecticides. The increases of expression of P450 genes at transcriptional level are often consider responsiblefor increasing the metabolism of insecticides and seems to be a common phenomenon in the evolutionof resistance development in insects. As pyrethroid resistance has been detected in Triatoma infestans, it was ofinterest to analyze genes associated with resistance to insecticides such as those encoding for cytochromesP450. With this purpose, the cDNA sequences of three cytochrome P450 genes (CYP4EM7, CYP3085B1, andCYP3092A6) were identified in this species. Primers and specific Taqman probes were designed from these sequencesto determine their expression by quantitative PCR. The mRNA levels of the cytochrome P450 genes identifiedwere determined from total RNA extracted from pools of fat body collected from individuals of differentresistant and susceptible strains of T. infestans, and at different interval times after the topical application ofthe lethal doses 50% (LD50) of deltamethrin on the ventral abdomen of insects belonging to the different populationsanalyzed. It was detected overexpression of the CYP4EM7 gene in the most resistant strain of T. infestans andthe expression of the three cytochrome P450 genes isolated was induced by deltamethrin in the susceptible andresistant populations included in this study. These results suggest that these genes would be involved in the detoxificationof deltamethrin and support the hypothesis that considers to the cytochrome P450 genes of importancein the development of pyrethroid resistance.
Fil: Grosso, Carla Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Blariza, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Mougabure Cueto, Gastón Adolfo. Ministerio de Salud. Dirección de Enfermedades Transmisibles Por Vectores. Centro de Referencia de Vectores; Argentina
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Garcia, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Materia
Triatoma Infestans
Chagas´ Disease Vector
P450 Genes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44564
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44564
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populationsGrosso, Carla GiselaBlariza, María JoséMougabure Cueto, Gastón AdolfoPicollo, Maria InesGarcia, Beatriz AliciaTriatoma InfestansChagas´ Disease VectorP450 Geneshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cytochrome P450 monooxygenases play a predominant role in the metabolism of insecticides. Many insect P450genes have frequently been associated with detoxification processes allowing the insect to become tolerant orresistant to insecticides. The increases of expression of P450 genes at transcriptional level are often consider responsiblefor increasing the metabolism of insecticides and seems to be a common phenomenon in the evolutionof resistance development in insects. As pyrethroid resistance has been detected in Triatoma infestans, it was ofinterest to analyze genes associated with resistance to insecticides such as those encoding for cytochromesP450. With this purpose, the cDNA sequences of three cytochrome P450 genes (CYP4EM7, CYP3085B1, andCYP3092A6) were identified in this species. Primers and specific Taqman probes were designed from these sequencesto determine their expression by quantitative PCR. The mRNA levels of the cytochrome P450 genes identifiedwere determined from total RNA extracted from pools of fat body collected from individuals of differentresistant and susceptible strains of T. infestans, and at different interval times after the topical application ofthe lethal doses 50% (LD50) of deltamethrin on the ventral abdomen of insects belonging to the different populationsanalyzed. It was detected overexpression of the CYP4EM7 gene in the most resistant strain of T. infestans andthe expression of the three cytochrome P450 genes isolated was induced by deltamethrin in the susceptible andresistant populations included in this study. These results suggest that these genes would be involved in the detoxificationof deltamethrin and support the hypothesis that considers to the cytochrome P450 genes of importancein the development of pyrethroid resistance.Fil: Grosso, Carla Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Blariza, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Mougabure Cueto, Gastón Adolfo. Ministerio de Salud. Dirección de Enfermedades Transmisibles Por Vectores. Centro de Referencia de Vectores; ArgentinaFil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Garcia, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaElsevier Science2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44564Grosso, Carla Gisela; Blariza, María José; Mougabure Cueto, Gastón Adolfo; Picollo, Maria Ines; Garcia, Beatriz Alicia; Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations; Elsevier Science; Infection, Genetics and Evolution; 44; 5-2016; 459-4701567-1348CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2016.07.027info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134816303161info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:00Zoai:ri.conicet.gov.ar:11336/44564instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:00.679CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
title Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
spellingShingle Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
Grosso, Carla Gisela
Triatoma Infestans
Chagas´ Disease Vector
P450 Genes
title_short Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
title_full Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
title_fullStr Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
title_full_unstemmed Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
title_sort Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations
dc.creator.none.fl_str_mv Grosso, Carla Gisela
Blariza, María José
Mougabure Cueto, Gastón Adolfo
Picollo, Maria Ines
Garcia, Beatriz Alicia
author Grosso, Carla Gisela
author_facet Grosso, Carla Gisela
Blariza, María José
Mougabure Cueto, Gastón Adolfo
Picollo, Maria Ines
Garcia, Beatriz Alicia
author_role author
author2 Blariza, María José
Mougabure Cueto, Gastón Adolfo
Picollo, Maria Ines
Garcia, Beatriz Alicia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Triatoma Infestans
Chagas´ Disease Vector
P450 Genes
topic Triatoma Infestans
Chagas´ Disease Vector
P450 Genes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cytochrome P450 monooxygenases play a predominant role in the metabolism of insecticides. Many insect P450genes have frequently been associated with detoxification processes allowing the insect to become tolerant orresistant to insecticides. The increases of expression of P450 genes at transcriptional level are often consider responsiblefor increasing the metabolism of insecticides and seems to be a common phenomenon in the evolutionof resistance development in insects. As pyrethroid resistance has been detected in Triatoma infestans, it was ofinterest to analyze genes associated with resistance to insecticides such as those encoding for cytochromesP450. With this purpose, the cDNA sequences of three cytochrome P450 genes (CYP4EM7, CYP3085B1, andCYP3092A6) were identified in this species. Primers and specific Taqman probes were designed from these sequencesto determine their expression by quantitative PCR. The mRNA levels of the cytochrome P450 genes identifiedwere determined from total RNA extracted from pools of fat body collected from individuals of differentresistant and susceptible strains of T. infestans, and at different interval times after the topical application ofthe lethal doses 50% (LD50) of deltamethrin on the ventral abdomen of insects belonging to the different populationsanalyzed. It was detected overexpression of the CYP4EM7 gene in the most resistant strain of T. infestans andthe expression of the three cytochrome P450 genes isolated was induced by deltamethrin in the susceptible andresistant populations included in this study. These results suggest that these genes would be involved in the detoxificationof deltamethrin and support the hypothesis that considers to the cytochrome P450 genes of importancein the development of pyrethroid resistance.
Fil: Grosso, Carla Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Blariza, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Mougabure Cueto, Gastón Adolfo. Ministerio de Salud. Dirección de Enfermedades Transmisibles Por Vectores. Centro de Referencia de Vectores; Argentina
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Garcia, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
description Cytochrome P450 monooxygenases play a predominant role in the metabolism of insecticides. Many insect P450genes have frequently been associated with detoxification processes allowing the insect to become tolerant orresistant to insecticides. The increases of expression of P450 genes at transcriptional level are often consider responsiblefor increasing the metabolism of insecticides and seems to be a common phenomenon in the evolutionof resistance development in insects. As pyrethroid resistance has been detected in Triatoma infestans, it was ofinterest to analyze genes associated with resistance to insecticides such as those encoding for cytochromesP450. With this purpose, the cDNA sequences of three cytochrome P450 genes (CYP4EM7, CYP3085B1, andCYP3092A6) were identified in this species. Primers and specific Taqman probes were designed from these sequencesto determine their expression by quantitative PCR. The mRNA levels of the cytochrome P450 genes identifiedwere determined from total RNA extracted from pools of fat body collected from individuals of differentresistant and susceptible strains of T. infestans, and at different interval times after the topical application ofthe lethal doses 50% (LD50) of deltamethrin on the ventral abdomen of insects belonging to the different populationsanalyzed. It was detected overexpression of the CYP4EM7 gene in the most resistant strain of T. infestans andthe expression of the three cytochrome P450 genes isolated was induced by deltamethrin in the susceptible andresistant populations included in this study. These results suggest that these genes would be involved in the detoxificationof deltamethrin and support the hypothesis that considers to the cytochrome P450 genes of importancein the development of pyrethroid resistance.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44564
Grosso, Carla Gisela; Blariza, María José; Mougabure Cueto, Gastón Adolfo; Picollo, Maria Ines; Garcia, Beatriz Alicia; Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations; Elsevier Science; Infection, Genetics and Evolution; 44; 5-2016; 459-470
1567-1348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44564
identifier_str_mv Grosso, Carla Gisela; Blariza, María José; Mougabure Cueto, Gastón Adolfo; Picollo, Maria Ines; Garcia, Beatriz Alicia; Identification of three cytochrome P450 genes in the Chagas' disease vector Triatoma infestans : Expression analysis in deltamethrin susceptible and resistant populations; Elsevier Science; Infection, Genetics and Evolution; 44; 5-2016; 459-470
1567-1348
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2016.07.027
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134816303161
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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