Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans

Autores
Dulbecco, Andrea Belén; Moriconi, Débora Elizabeth; Calderón Fernández, Gustavo Mario; Lynn, María Soledad; McCarthy, Andrés Norman; Roca Acevedo, Gonzalo; Salamanca Moreno, Jhon A.; Juárez, Marta Patricia; Pedrini, Nicolás
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans.
Instituto de Investigaciones Bioquímicas de La Plata
Centro Regional de Estudios Genómicos
Materia
Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/97449
Acceder
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network_name_str SEDICI (UNLP)
spelling Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestansDulbecco, Andrea BelénMoriconi, Débora ElizabethCalderón Fernández, Gustavo MarioLynn, María SoledadMcCarthy, Andrés NormanRoca Acevedo, GonzaloSalamanca Moreno, Jhon A.Juárez, Marta PatriciaPedrini, NicolásBioquímicaBiologíaCiencias NaturalesCiencias ExactasTriatoma infestansCytochrome p450Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans.Instituto de Investigaciones Bioquímicas de La PlataCentro Regional de Estudios Genómicos2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-12http://sedici.unlp.edu.ar/handle/10915/97449enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/83006info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-28475-xinfo:eu-repo/semantics/altIdentifier/issn/1567-1348info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-28475-xinfo:eu-repo/semantics/altIdentifier/hdl/11336/83006info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:12:23Zoai:sedici.unlp.edu.ar:10915/97449Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:12:24.136SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
spellingShingle Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
Dulbecco, Andrea Belén
Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
title_short Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_full Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_fullStr Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_full_unstemmed Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
title_sort Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
dc.creator.none.fl_str_mv Dulbecco, Andrea Belén
Moriconi, Débora Elizabeth
Calderón Fernández, Gustavo Mario
Lynn, María Soledad
McCarthy, Andrés Norman
Roca Acevedo, Gonzalo
Salamanca Moreno, Jhon A.
Juárez, Marta Patricia
Pedrini, Nicolás
author Dulbecco, Andrea Belén
author_facet Dulbecco, Andrea Belén
Moriconi, Débora Elizabeth
Calderón Fernández, Gustavo Mario
Lynn, María Soledad
McCarthy, Andrés Norman
Roca Acevedo, Gonzalo
Salamanca Moreno, Jhon A.
Juárez, Marta Patricia
Pedrini, Nicolás
author_role author
author2 Moriconi, Débora Elizabeth
Calderón Fernández, Gustavo Mario
Lynn, María Soledad
McCarthy, Andrés Norman
Roca Acevedo, Gonzalo
Salamanca Moreno, Jhon A.
Juárez, Marta Patricia
Pedrini, Nicolás
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
topic Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450
dc.description.none.fl_txt_mv Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans.
Instituto de Investigaciones Bioquímicas de La Plata
Centro Regional de Estudios Genómicos
description Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/97449
url http://sedici.unlp.edu.ar/handle/10915/97449
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/83006
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-28475-x
info:eu-repo/semantics/altIdentifier/issn/1567-1348
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-28475-x
info:eu-repo/semantics/altIdentifier/hdl/11336/83006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1-12
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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