Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans
- Autores
- Dulbecco, Andrea Belén; Moriconi, Débora Elizabeth; Calderón Fernández, Gustavo Mario; Lynn, María Soledad; McCarthy, Andrés Norman; Roca Acevedo, Gonzalo; Salamanca Moreno, Jhon A.; Juárez, Marta Patricia; Pedrini, Nicolás
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans.
Instituto de Investigaciones Bioquímicas de La Plata
Centro Regional de Estudios Genómicos - Materia
-
Bioquímica
Biología
Ciencias Naturales
Ciencias Exactas
Triatoma infestans
Cytochrome p450 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/97449
Ver los metadatos del registro completo
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Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestansDulbecco, Andrea BelénMoriconi, Débora ElizabethCalderón Fernández, Gustavo MarioLynn, María SoledadMcCarthy, Andrés NormanRoca Acevedo, GonzaloSalamanca Moreno, Jhon A.Juárez, Marta PatriciaPedrini, NicolásBioquímicaBiologíaCiencias NaturalesCiencias ExactasTriatoma infestansCytochrome p450Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans.Instituto de Investigaciones Bioquímicas de La PlataCentro Regional de Estudios Genómicos2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-12http://sedici.unlp.edu.ar/handle/10915/97449enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/83006info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-28475-xinfo:eu-repo/semantics/altIdentifier/issn/1567-1348info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-28475-xinfo:eu-repo/semantics/altIdentifier/hdl/11336/83006info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:12:23Zoai:sedici.unlp.edu.ar:10915/97449Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:12:24.136SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
title |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
spellingShingle |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans Dulbecco, Andrea Belén Bioquímica Biología Ciencias Naturales Ciencias Exactas Triatoma infestans Cytochrome p450 |
title_short |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
title_full |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
title_fullStr |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
title_full_unstemmed |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
title_sort |
Integument CYP genes of the largest genome-wide cytochrome P450 expansions in triatomines participate in detoxification in deltamethrin-resistant Triatoma infestans |
dc.creator.none.fl_str_mv |
Dulbecco, Andrea Belén Moriconi, Débora Elizabeth Calderón Fernández, Gustavo Mario Lynn, María Soledad McCarthy, Andrés Norman Roca Acevedo, Gonzalo Salamanca Moreno, Jhon A. Juárez, Marta Patricia Pedrini, Nicolás |
author |
Dulbecco, Andrea Belén |
author_facet |
Dulbecco, Andrea Belén Moriconi, Débora Elizabeth Calderón Fernández, Gustavo Mario Lynn, María Soledad McCarthy, Andrés Norman Roca Acevedo, Gonzalo Salamanca Moreno, Jhon A. Juárez, Marta Patricia Pedrini, Nicolás |
author_role |
author |
author2 |
Moriconi, Débora Elizabeth Calderón Fernández, Gustavo Mario Lynn, María Soledad McCarthy, Andrés Norman Roca Acevedo, Gonzalo Salamanca Moreno, Jhon A. Juárez, Marta Patricia Pedrini, Nicolás |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Bioquímica Biología Ciencias Naturales Ciencias Exactas Triatoma infestans Cytochrome p450 |
topic |
Bioquímica Biología Ciencias Naturales Ciencias Exactas Triatoma infestans Cytochrome p450 |
dc.description.none.fl_txt_mv |
Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans. Instituto de Investigaciones Bioquímicas de La Plata Centro Regional de Estudios Genómicos |
description |
Insect resistance to chemical insecticides is attributed to a combination of different mechanisms, such as metabolic resistance, knockdown resistance, and the cuticular resistance or penetration factor. The insect integument offers an efficient barrier against contact insecticides and its role as penetration factor has been previously reported; however, there is no information about its potential function in the metabolic resistance. Cytochrome P450 genes (CYP) are highly expressed in the fat body of several insects and thus play a key role in their metabolic resistance. Here, we describe new members that belong to the highly genome-wide expanded CYP3093A and CYP4EM subfamilies in the Chagas disease vectors Rhodnius prolixus and Triatoma infestans. We modeled the docking of deltamethrin in their active site and detected differences in some amino acids between both species that are critical for a correct interaction with the substrate. We also knocked down the two constitutively most expressed genes in the integument of resistant T. infestans nymphs (CYP3093A11 and CYP4EM10) in order to find clues on their participation in deltamethrin resistance. This is the first report on the role of the insect integument in detoxification events; although these two CYP genes do not fully explain the resistance observed in T. infestans. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/97449 |
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http://sedici.unlp.edu.ar/handle/10915/97449 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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