In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes

Autores
Trinks, Julieta; Sugiyama, Masaya; Tanaka, Yasuhito; Kurbanov, Fuat; Benetucci, Jorge; Giménez, Edgardo; Weissenbacher, Mercedes Crecencia; Mizokami, Masashi; Oubiña, Jose Raul
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hepatitis B virus (HBV) DNA recombinants contribute to ~30% of the overall full-length sequences already deposited in GenBank. However, their biological behaviour has not been analysed so far. In this study, the in vitro replication kinetics of the first D/A recombinant from the American continent differed from its parental genotypes, exhibiting higher extracellular levels of HBV DNA and hepatitis B e antigen. Southern blots of intracellular core-associated HBV DNA were in agreement with such results. Because this recombinant was obtained from an Argentinian injecting drug user belonging to a vulnerable community, these results are of singular relevance for regional public health. Further in vivo studies are urgently needed to determine the pathogenicity of these replicative competent clones.
Fil: Trinks, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Sugiyama, Masaya. Nagoya City University; Japón. National Center for Global Health and Medicine; Japón
Fil: Tanaka, Yasuhito. Nagoya City University; Japón
Fil: Kurbanov, Fuat. Nagoya City University; Japón
Fil: Benetucci, Jorge. Fundación de Ayuda al Inmunodeficiente; Argentina
Fil: Giménez, Edgardo. Hospital Zonal General de Agudos "Dr Isidoro G. Iriarte"; Argentina
Fil: Weissenbacher, Mercedes Crecencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Mizokami, Masashi. National Center for Global Health and Medicine; Japón
Fil: Oubiña, Jose Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Materia
HBV
RECOMBINANT
GENOTYPES
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89349
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/89349
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypesTrinks, JulietaSugiyama, MasayaTanaka, YasuhitoKurbanov, FuatBenetucci, JorgeGiménez, EdgardoWeissenbacher, Mercedes CrecenciaMizokami, MasashiOubiña, Jose RaulHBVRECOMBINANTGENOTYPESARGENTINAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Hepatitis B virus (HBV) DNA recombinants contribute to ~30% of the overall full-length sequences already deposited in GenBank. However, their biological behaviour has not been analysed so far. In this study, the in vitro replication kinetics of the first D/A recombinant from the American continent differed from its parental genotypes, exhibiting higher extracellular levels of HBV DNA and hepatitis B e antigen. Southern blots of intracellular core-associated HBV DNA were in agreement with such results. Because this recombinant was obtained from an Argentinian injecting drug user belonging to a vulnerable community, these results are of singular relevance for regional public health. Further in vivo studies are urgently needed to determine the pathogenicity of these replicative competent clones.Fil: Trinks, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Sugiyama, Masaya. Nagoya City University; Japón. National Center for Global Health and Medicine; JapónFil: Tanaka, Yasuhito. Nagoya City University; JapónFil: Kurbanov, Fuat. Nagoya City University; JapónFil: Benetucci, Jorge. Fundación de Ayuda al Inmunodeficiente; ArgentinaFil: Giménez, Edgardo. Hospital Zonal General de Agudos "Dr Isidoro G. Iriarte"; ArgentinaFil: Weissenbacher, Mercedes Crecencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Mizokami, Masashi. National Center for Global Health and Medicine; JapónFil: Oubiña, Jose Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaSociety for General Microbiology2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89349Trinks, Julieta; Sugiyama, Masaya; Tanaka, Yasuhito; Kurbanov, Fuat; Benetucci, Jorge; et al.; In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes; Society for General Microbiology; Journal of General Virology; 94; 12; 12-2013; 2724-27280022-1317CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.053595-0info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.053595-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:23Zoai:ri.conicet.gov.ar:11336/89349instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:25.555CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
title In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
spellingShingle In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
Trinks, Julieta
HBV
RECOMBINANT
GENOTYPES
ARGENTINA
title_short In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
title_full In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
title_fullStr In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
title_full_unstemmed In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
title_sort In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes
dc.creator.none.fl_str_mv Trinks, Julieta
Sugiyama, Masaya
Tanaka, Yasuhito
Kurbanov, Fuat
Benetucci, Jorge
Giménez, Edgardo
Weissenbacher, Mercedes Crecencia
Mizokami, Masashi
Oubiña, Jose Raul
author Trinks, Julieta
author_facet Trinks, Julieta
Sugiyama, Masaya
Tanaka, Yasuhito
Kurbanov, Fuat
Benetucci, Jorge
Giménez, Edgardo
Weissenbacher, Mercedes Crecencia
Mizokami, Masashi
Oubiña, Jose Raul
author_role author
author2 Sugiyama, Masaya
Tanaka, Yasuhito
Kurbanov, Fuat
Benetucci, Jorge
Giménez, Edgardo
Weissenbacher, Mercedes Crecencia
Mizokami, Masashi
Oubiña, Jose Raul
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HBV
RECOMBINANT
GENOTYPES
ARGENTINA
topic HBV
RECOMBINANT
GENOTYPES
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Hepatitis B virus (HBV) DNA recombinants contribute to ~30% of the overall full-length sequences already deposited in GenBank. However, their biological behaviour has not been analysed so far. In this study, the in vitro replication kinetics of the first D/A recombinant from the American continent differed from its parental genotypes, exhibiting higher extracellular levels of HBV DNA and hepatitis B e antigen. Southern blots of intracellular core-associated HBV DNA were in agreement with such results. Because this recombinant was obtained from an Argentinian injecting drug user belonging to a vulnerable community, these results are of singular relevance for regional public health. Further in vivo studies are urgently needed to determine the pathogenicity of these replicative competent clones.
Fil: Trinks, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Sugiyama, Masaya. Nagoya City University; Japón. National Center for Global Health and Medicine; Japón
Fil: Tanaka, Yasuhito. Nagoya City University; Japón
Fil: Kurbanov, Fuat. Nagoya City University; Japón
Fil: Benetucci, Jorge. Fundación de Ayuda al Inmunodeficiente; Argentina
Fil: Giménez, Edgardo. Hospital Zonal General de Agudos "Dr Isidoro G. Iriarte"; Argentina
Fil: Weissenbacher, Mercedes Crecencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Mizokami, Masashi. National Center for Global Health and Medicine; Japón
Fil: Oubiña, Jose Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
description Hepatitis B virus (HBV) DNA recombinants contribute to ~30% of the overall full-length sequences already deposited in GenBank. However, their biological behaviour has not been analysed so far. In this study, the in vitro replication kinetics of the first D/A recombinant from the American continent differed from its parental genotypes, exhibiting higher extracellular levels of HBV DNA and hepatitis B e antigen. Southern blots of intracellular core-associated HBV DNA were in agreement with such results. Because this recombinant was obtained from an Argentinian injecting drug user belonging to a vulnerable community, these results are of singular relevance for regional public health. Further in vivo studies are urgently needed to determine the pathogenicity of these replicative competent clones.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89349
Trinks, Julieta; Sugiyama, Masaya; Tanaka, Yasuhito; Kurbanov, Fuat; Benetucci, Jorge; et al.; In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes; Society for General Microbiology; Journal of General Virology; 94; 12; 12-2013; 2724-2728
0022-1317
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89349
identifier_str_mv Trinks, Julieta; Sugiyama, Masaya; Tanaka, Yasuhito; Kurbanov, Fuat; Benetucci, Jorge; et al.; In vitro replication competence of a hepatitis B genotype D/A recombinant virus: Dissimilar biological behaviour regarding its parental genotypes; Society for General Microbiology; Journal of General Virology; 94; 12; 12-2013; 2724-2728
0022-1317
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/vir.0.053595-0
info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.053595-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268972182929408
score 13.13397

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