Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
- Autores
- Méndez Ubach, Milagros; Hernández Fonseca, Karla; Abate, Paula
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways.
Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Hernández Fonseca, Karla. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research
Córdoba
Argentina
Latin American Society for Biomedical Research on Alcoholism - Materia
-
Ethanol
Prenatal exposure
Locomotor response
Met-enkephalin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/170154
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Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent ratsMéndez Ubach, MilagrosHernández Fonseca, KarlaAbate, PaulaEthanolPrenatal exposureLocomotor responseMet-enkephalinhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways.Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; MéxicoFil: Hernández Fonseca, Karla. Instituto Nacional de Psiquiatría Ramón de la Fuente; MéxicoFil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; ArgentinaIX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic researchCórdobaArgentinaLatin American Society for Biomedical Research on AlcoholismDougmar Group2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170154Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 58-58CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jfasrp.com/index.php/JFASRP/article/view/7Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:15Zoai:ri.conicet.gov.ar:11336/170154instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:15.227CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats |
title |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats |
spellingShingle |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats Méndez Ubach, Milagros Ethanol Prenatal exposure Locomotor response Met-enkephalin |
title_short |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats |
title_full |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats |
title_fullStr |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats |
title_full_unstemmed |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats |
title_sort |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats |
dc.creator.none.fl_str_mv |
Méndez Ubach, Milagros Hernández Fonseca, Karla Abate, Paula |
author |
Méndez Ubach, Milagros |
author_facet |
Méndez Ubach, Milagros Hernández Fonseca, Karla Abate, Paula |
author_role |
author |
author2 |
Hernández Fonseca, Karla Abate, Paula |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Ethanol Prenatal exposure Locomotor response Met-enkephalin |
topic |
Ethanol Prenatal exposure Locomotor response Met-enkephalin |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways. Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; México Fil: Hernández Fonseca, Karla. Instituto Nacional de Psiquiatría Ramón de la Fuente; México Fil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research Córdoba Argentina Latin American Society for Biomedical Research on Alcoholism |
description |
Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Encuentro Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/170154 Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 58-58 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/170154 |
identifier_str_mv |
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 58-58 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.jfasrp.com/index.php/JFASRP/article/view/7 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Dougmar Group |
publisher.none.fl_str_mv |
Dougmar Group |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |