Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats

Autores
Méndez Ubach, Milagros; Hernández Fonseca, Karla; Abate, Paula
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways.
Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Hernández Fonseca, Karla. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research
Córdoba
Argentina
Latin American Society for Biomedical Research on Alcoholism
Materia
Ethanol
Prenatal exposure
Locomotor response
Met-enkephalin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170154

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oai_identifier_str oai:ri.conicet.gov.ar:11336/170154
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network_name_str CONICET Digital (CONICET)
spelling Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent ratsMéndez Ubach, MilagrosHernández Fonseca, KarlaAbate, PaulaEthanolPrenatal exposureLocomotor responseMet-enkephalinhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways.Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; MéxicoFil: Hernández Fonseca, Karla. Instituto Nacional de Psiquiatría Ramón de la Fuente; MéxicoFil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; ArgentinaIX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic researchCórdobaArgentinaLatin American Society for Biomedical Research on AlcoholismDougmar Group2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170154Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 58-58CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jfasrp.com/index.php/JFASRP/article/view/7Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:15Zoai:ri.conicet.gov.ar:11336/170154instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:15.227CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
title Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
spellingShingle Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
Méndez Ubach, Milagros
Ethanol
Prenatal exposure
Locomotor response
Met-enkephalin
title_short Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
title_full Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
title_fullStr Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
title_full_unstemmed Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
title_sort Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats
dc.creator.none.fl_str_mv Méndez Ubach, Milagros
Hernández Fonseca, Karla
Abate, Paula
author Méndez Ubach, Milagros
author_facet Méndez Ubach, Milagros
Hernández Fonseca, Karla
Abate, Paula
author_role author
author2 Hernández Fonseca, Karla
Abate, Paula
author2_role author
author
dc.subject.none.fl_str_mv Ethanol
Prenatal exposure
Locomotor response
Met-enkephalin
topic Ethanol
Prenatal exposure
Locomotor response
Met-enkephalin
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways.
Fil: Méndez Ubach, Milagros. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Hernández Fonseca, Karla. Instituto Nacional de Psiquiatría Ramón de la Fuente; México
Fil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research
Córdoba
Argentina
Latin American Society for Biomedical Research on Alcoholism
description Prenatal ethanol exposure (PEE) facilitates alcohol acceptance and intake, suggesting that ethanol in uteromay increase the probability of drug abuse during adolescence and adulthood. Alcohol reinforcement involves the ethanol-induced activation of opioidergic systems in mesocorticolimbic areas. Changes in opioidneurotransmission may be relevant during ethanol intoxication, as well as in the adaptive neural responsesinduced by the drug. Some studies have assessed the possible changes in opioidergic systems as a functionof ethanol exposure in adolescent animals. However, PEE effects upon locomotive responses elicited by anethanol challenge and modulation of neurotransmission of opioidergic systems remain to be understood. Thiswork assessed the susceptibility of adolescent rats to prenatal and/or postnatal ethanol exposure in terms oflocomotive responses, as well as alcohol-related effects on Methionine-enkephalin (Met-enk) expression inbrain areas related to drug reinforcement. Pregnant rats received a daily intragastric administration of ethanol(2 g/kg) or water, during gestational days 17-20. Adolescents at postnatal day 30 (PD30) were tested in afirst baseline trial (habituation session) and evaluated in terms of spontaneous activity. Thereafter, animalsreceived an ip injection of vehicle (saline 0.9% w/v) (vehicle session) and were immediately evaluated interms of activity during 30 min. After this second trial, animals from both prenatal treatments were injectedwith ethanol (1.0 g/kg ip) or saline, and locomotor activity was immediately assessed for 30 min (drug session). Met-enk content was quantitated by radioimmunoassay in several brain regions: ventral tegmental area[VTA], nucleus accumbens [NAcc], prefrontal cortex [PFC], substantia nigra [SN], caudate-putamen [CP],amygdala, hypothalamus and hippocampus. PEE significantly reduced rearing responses. Ethanol challenge atPD30 decreased horizontal locomotion and showed a tendency to reduce rearings and stereotyped behaviors.PEE increased Met-enk content in the PFC, CP, hypothalamus and hippocampus, but did not alter peptidelevels in the amygdala, VTA and NAcc. These findings suggest that PEE selectively modifies behavioralparameters at PD30 and induces specific changes in Met-enk content in regions of the mesocortical andnigrostriatal pathways, the hypothalamus and hippocampus. Prenatal and postnatal ethanol actions on motoractivity in adolescents could involve activation of specific neural enkephalinergic pathways.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Encuentro
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170154
Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 58-58
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170154
identifier_str_mv Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-Enkephalin content in adolescent rats; IX International Meeting of the Latin American Society for Biomedical Research on Alcoholism: Determinants of Alcoholism: Bridging the gap between epidemiological and basic research; Córdoba; Argentina; 2019; 58-58
CONICET Digital
CONICET
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language eng
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https://creativecommons.org/licenses/by-nc/2.5/ar/
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dc.publisher.none.fl_str_mv Dougmar Group
publisher.none.fl_str_mv Dougmar Group
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