DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase

Autores
Soria, Ramiro Gasto; Belluscio, Laura María; van Cappellen, W. A.; Kanaar, Roland; Essers, Jeroen; Gottifredi, Vanesa
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
When DNA is damaged in cells progressing through S phase, replication blockage can be avoided by TLS (Translesion DNA synthesis). This is an auxiliary replication mechanism that relies on the function of specialized polymerases that accomplish DNA damage bypass. Intriguingly, recent evidence has linked TLS polymerases to processes that can also take place outside S phase such as nucleotide excision repair (NER). Here we show that Pol eta is recruited to UV-induced DNA lesions in cells outside S phase including cells permanently arrested in G(1). This observation was confirmed by different strategies including global UV irradiation, local UV irradiation and local multi-photon laser irradiation of single nuclei in living cells. The potential connection between Pol eta recruitment to DNA lesions outside S phase and NER was further evaluated. Interestingly, the recruitment of Pol eta to damage sites outside S phase did not depend on active NER, as UV-induced focus formation occurred normally in XPA, XPG and XPF deficient fibroblasts. Our data reveals that the re-localization of the TLS polymerase Pol eta to photo-lesions might be temporally and mechanistically uncoupled from replicative DNA synthesis and from DNA damage processing.
Fil: Soria, Ramiro Gasto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Belluscio, Laura María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: van Cappellen, W. A.. Erasmus MC; Países Bajos
Fil: Kanaar, Roland. Erasmus MC; Países Bajos
Fil: Essers, Jeroen. Erasmus MC; Países Bajos
Fil: Gottifredi, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Materia
Pcna
Pol H (Eta)
Cell Cycle
Tranlesion Dna Synthesis (Tls)
Nucleotide Excision Repair (Ner)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25933
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25933
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA damage induced Pol eta recruitment takes place independently of the cell cycle phaseSoria, Ramiro GastoBelluscio, Laura Maríavan Cappellen, W. A.Kanaar, RolandEssers, JeroenGottifredi, VanesaPcnaPol H (Eta)Cell CycleTranlesion Dna Synthesis (Tls)Nucleotide Excision Repair (Ner)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1When DNA is damaged in cells progressing through S phase, replication blockage can be avoided by TLS (Translesion DNA synthesis). This is an auxiliary replication mechanism that relies on the function of specialized polymerases that accomplish DNA damage bypass. Intriguingly, recent evidence has linked TLS polymerases to processes that can also take place outside S phase such as nucleotide excision repair (NER). Here we show that Pol eta is recruited to UV-induced DNA lesions in cells outside S phase including cells permanently arrested in G(1). This observation was confirmed by different strategies including global UV irradiation, local UV irradiation and local multi-photon laser irradiation of single nuclei in living cells. The potential connection between Pol eta recruitment to DNA lesions outside S phase and NER was further evaluated. Interestingly, the recruitment of Pol eta to damage sites outside S phase did not depend on active NER, as UV-induced focus formation occurred normally in XPA, XPG and XPF deficient fibroblasts. Our data reveals that the re-localization of the TLS polymerase Pol eta to photo-lesions might be temporally and mechanistically uncoupled from replicative DNA synthesis and from DNA damage processing.Fil: Soria, Ramiro Gasto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; ArgentinaFil: Belluscio, Laura María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; ArgentinaFil: van Cappellen, W. A.. Erasmus MC; Países BajosFil: Kanaar, Roland. Erasmus MC; Países BajosFil: Essers, Jeroen. Erasmus MC; Países BajosFil: Gottifredi, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; ArgentinaLandes Bioscience2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25933Soria, Ramiro Gasto; Belluscio, Laura María; van Cappellen, W. A.; Kanaar, Roland; Essers, Jeroen; et al.; DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase; Landes Bioscience; Cell Cycle; 8; 20; 10-2009; 3340-33481538-41011551-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.4161/cc.8.20.9836info:eu-repo/semantics/altIdentifier/doi/10.4161/cc.8.20.9836info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:58Zoai:ri.conicet.gov.ar:11336/25933instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:59.063CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
title DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
spellingShingle DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
Soria, Ramiro Gasto
Pcna
Pol H (Eta)
Cell Cycle
Tranlesion Dna Synthesis (Tls)
Nucleotide Excision Repair (Ner)
title_short DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
title_full DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
title_fullStr DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
title_full_unstemmed DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
title_sort DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase
dc.creator.none.fl_str_mv Soria, Ramiro Gasto
Belluscio, Laura María
van Cappellen, W. A.
Kanaar, Roland
Essers, Jeroen
Gottifredi, Vanesa
author Soria, Ramiro Gasto
author_facet Soria, Ramiro Gasto
Belluscio, Laura María
van Cappellen, W. A.
Kanaar, Roland
Essers, Jeroen
Gottifredi, Vanesa
author_role author
author2 Belluscio, Laura María
van Cappellen, W. A.
Kanaar, Roland
Essers, Jeroen
Gottifredi, Vanesa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pcna
Pol H (Eta)
Cell Cycle
Tranlesion Dna Synthesis (Tls)
Nucleotide Excision Repair (Ner)
topic Pcna
Pol H (Eta)
Cell Cycle
Tranlesion Dna Synthesis (Tls)
Nucleotide Excision Repair (Ner)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv When DNA is damaged in cells progressing through S phase, replication blockage can be avoided by TLS (Translesion DNA synthesis). This is an auxiliary replication mechanism that relies on the function of specialized polymerases that accomplish DNA damage bypass. Intriguingly, recent evidence has linked TLS polymerases to processes that can also take place outside S phase such as nucleotide excision repair (NER). Here we show that Pol eta is recruited to UV-induced DNA lesions in cells outside S phase including cells permanently arrested in G(1). This observation was confirmed by different strategies including global UV irradiation, local UV irradiation and local multi-photon laser irradiation of single nuclei in living cells. The potential connection between Pol eta recruitment to DNA lesions outside S phase and NER was further evaluated. Interestingly, the recruitment of Pol eta to damage sites outside S phase did not depend on active NER, as UV-induced focus formation occurred normally in XPA, XPG and XPF deficient fibroblasts. Our data reveals that the re-localization of the TLS polymerase Pol eta to photo-lesions might be temporally and mechanistically uncoupled from replicative DNA synthesis and from DNA damage processing.
Fil: Soria, Ramiro Gasto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: Belluscio, Laura María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
Fil: van Cappellen, W. A.. Erasmus MC; Países Bajos
Fil: Kanaar, Roland. Erasmus MC; Países Bajos
Fil: Essers, Jeroen. Erasmus MC; Países Bajos
Fil: Gottifredi, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires; Argentina
description When DNA is damaged in cells progressing through S phase, replication blockage can be avoided by TLS (Translesion DNA synthesis). This is an auxiliary replication mechanism that relies on the function of specialized polymerases that accomplish DNA damage bypass. Intriguingly, recent evidence has linked TLS polymerases to processes that can also take place outside S phase such as nucleotide excision repair (NER). Here we show that Pol eta is recruited to UV-induced DNA lesions in cells outside S phase including cells permanently arrested in G(1). This observation was confirmed by different strategies including global UV irradiation, local UV irradiation and local multi-photon laser irradiation of single nuclei in living cells. The potential connection between Pol eta recruitment to DNA lesions outside S phase and NER was further evaluated. Interestingly, the recruitment of Pol eta to damage sites outside S phase did not depend on active NER, as UV-induced focus formation occurred normally in XPA, XPG and XPF deficient fibroblasts. Our data reveals that the re-localization of the TLS polymerase Pol eta to photo-lesions might be temporally and mechanistically uncoupled from replicative DNA synthesis and from DNA damage processing.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25933
Soria, Ramiro Gasto; Belluscio, Laura María; van Cappellen, W. A.; Kanaar, Roland; Essers, Jeroen; et al.; DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase; Landes Bioscience; Cell Cycle; 8; 20; 10-2009; 3340-3348
1538-4101
1551-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25933
identifier_str_mv Soria, Ramiro Gasto; Belluscio, Laura María; van Cappellen, W. A.; Kanaar, Roland; Essers, Jeroen; et al.; DNA damage induced Pol eta recruitment takes place independently of the cell cycle phase; Landes Bioscience; Cell Cycle; 8; 20; 10-2009; 3340-3348
1538-4101
1551-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.4161/cc.8.20.9836
info:eu-repo/semantics/altIdentifier/doi/10.4161/cc.8.20.9836
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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