Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants

Autores
Jaime, Camila Lourdes; Muchut, Sebastián Elías; Reutemann Arnolfo, Andrea Guadalupe; Gieco, J.; Dunger, Ricardo German
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, infections by alfalfa dwarf virus (ADV) affect the cultivation of alfalfa, which is globally one of the most important forage plants. The main objective of this study was to improve current understanding of the underlying mechanisms related to the dwarfism phenotype developed during viral infection. Hydrogen peroxide production, callose deposition and PR5 gene expression levels were evaluated to determine if ADV induces plant defence responses. At the morphological level, higher epicuticular wax production and an increase in proliferation of cells from the fundamental parenchyma were observed when plants were infected by ADV. Infected plants had reduced photosynthesis, stomatal conductance and transpiration/evaporation rate, but interestingly the production of chlorophylls was induced. Finally, using transcriptional analysis, ADV was observed to negatively affect the expression of genes related to synthesis of auxins, cytokinins and brassinosteroids. These results suggest that ADV infection induces a hormonal imbalance leading to an increase in chlorophyll pigment synthesis, stomatal closure and generation of tissue deformation. Overall, the results show a morphological, physiological and photosynthetic characterization of dwarf plants affected by ADV.
Fil: Jaime, Camila Lourdes. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Muchut, Sebastián Elías. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Reutemann Arnolfo, Andrea Guadalupe. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Gieco, J.. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; Argentina
Fil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; Argentina
Materia
CHLOROPHYLL
DWARFISM
FORAGE
PHYTOHORMONES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151911
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/151911
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plantsJaime, Camila LourdesMuchut, Sebastián ElíasReutemann Arnolfo, Andrea GuadalupeGieco, J.Dunger, Ricardo GermanCHLOROPHYLLDWARFISMFORAGEPHYTOHORMONEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Argentina, infections by alfalfa dwarf virus (ADV) affect the cultivation of alfalfa, which is globally one of the most important forage plants. The main objective of this study was to improve current understanding of the underlying mechanisms related to the dwarfism phenotype developed during viral infection. Hydrogen peroxide production, callose deposition and PR5 gene expression levels were evaluated to determine if ADV induces plant defence responses. At the morphological level, higher epicuticular wax production and an increase in proliferation of cells from the fundamental parenchyma were observed when plants were infected by ADV. Infected plants had reduced photosynthesis, stomatal conductance and transpiration/evaporation rate, but interestingly the production of chlorophylls was induced. Finally, using transcriptional analysis, ADV was observed to negatively affect the expression of genes related to synthesis of auxins, cytokinins and brassinosteroids. These results suggest that ADV infection induces a hormonal imbalance leading to an increase in chlorophyll pigment synthesis, stomatal closure and generation of tissue deformation. Overall, the results show a morphological, physiological and photosynthetic characterization of dwarf plants affected by ADV.Fil: Jaime, Camila Lourdes. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Muchut, Sebastián Elías. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Reutemann Arnolfo, Andrea Guadalupe. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Gieco, J.. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; ArgentinaFil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; ArgentinaWiley Blackwell Publishing, Inc2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151911Jaime, Camila Lourdes; Muchut, Sebastián Elías; Reutemann Arnolfo, Andrea Guadalupe; Gieco, J.; Dunger, Ricardo German; Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants; Wiley Blackwell Publishing, Inc; Plant Pathology; 69; 2; 10-2019; 393-4020032-0862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13109info:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.13109info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:28Zoai:ri.conicet.gov.ar:11336/151911instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:28.707CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
title Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
spellingShingle Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
Jaime, Camila Lourdes
CHLOROPHYLL
DWARFISM
FORAGE
PHYTOHORMONES
title_short Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
title_full Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
title_fullStr Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
title_full_unstemmed Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
title_sort Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants
dc.creator.none.fl_str_mv Jaime, Camila Lourdes
Muchut, Sebastián Elías
Reutemann Arnolfo, Andrea Guadalupe
Gieco, J.
Dunger, Ricardo German
author Jaime, Camila Lourdes
author_facet Jaime, Camila Lourdes
Muchut, Sebastián Elías
Reutemann Arnolfo, Andrea Guadalupe
Gieco, J.
Dunger, Ricardo German
author_role author
author2 Muchut, Sebastián Elías
Reutemann Arnolfo, Andrea Guadalupe
Gieco, J.
Dunger, Ricardo German
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHLOROPHYLL
DWARFISM
FORAGE
PHYTOHORMONES
topic CHLOROPHYLL
DWARFISM
FORAGE
PHYTOHORMONES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Argentina, infections by alfalfa dwarf virus (ADV) affect the cultivation of alfalfa, which is globally one of the most important forage plants. The main objective of this study was to improve current understanding of the underlying mechanisms related to the dwarfism phenotype developed during viral infection. Hydrogen peroxide production, callose deposition and PR5 gene expression levels were evaluated to determine if ADV induces plant defence responses. At the morphological level, higher epicuticular wax production and an increase in proliferation of cells from the fundamental parenchyma were observed when plants were infected by ADV. Infected plants had reduced photosynthesis, stomatal conductance and transpiration/evaporation rate, but interestingly the production of chlorophylls was induced. Finally, using transcriptional analysis, ADV was observed to negatively affect the expression of genes related to synthesis of auxins, cytokinins and brassinosteroids. These results suggest that ADV infection induces a hormonal imbalance leading to an increase in chlorophyll pigment synthesis, stomatal closure and generation of tissue deformation. Overall, the results show a morphological, physiological and photosynthetic characterization of dwarf plants affected by ADV.
Fil: Jaime, Camila Lourdes. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Muchut, Sebastián Elías. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Reutemann Arnolfo, Andrea Guadalupe. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Gieco, J.. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; Argentina
Fil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Departamento de Producción Animal; Argentina
description In Argentina, infections by alfalfa dwarf virus (ADV) affect the cultivation of alfalfa, which is globally one of the most important forage plants. The main objective of this study was to improve current understanding of the underlying mechanisms related to the dwarfism phenotype developed during viral infection. Hydrogen peroxide production, callose deposition and PR5 gene expression levels were evaluated to determine if ADV induces plant defence responses. At the morphological level, higher epicuticular wax production and an increase in proliferation of cells from the fundamental parenchyma were observed when plants were infected by ADV. Infected plants had reduced photosynthesis, stomatal conductance and transpiration/evaporation rate, but interestingly the production of chlorophylls was induced. Finally, using transcriptional analysis, ADV was observed to negatively affect the expression of genes related to synthesis of auxins, cytokinins and brassinosteroids. These results suggest that ADV infection induces a hormonal imbalance leading to an increase in chlorophyll pigment synthesis, stomatal closure and generation of tissue deformation. Overall, the results show a morphological, physiological and photosynthetic characterization of dwarf plants affected by ADV.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151911
Jaime, Camila Lourdes; Muchut, Sebastián Elías; Reutemann Arnolfo, Andrea Guadalupe; Gieco, J.; Dunger, Ricardo German; Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants; Wiley Blackwell Publishing, Inc; Plant Pathology; 69; 2; 10-2019; 393-402
0032-0862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151911
identifier_str_mv Jaime, Camila Lourdes; Muchut, Sebastián Elías; Reutemann Arnolfo, Andrea Guadalupe; Gieco, J.; Dunger, Ricardo German; Morphological changes, alteration of photosynthetic parameters and chlorophyll production induced by infection with alfalfa dwarf virus in Medicago sativa plants; Wiley Blackwell Publishing, Inc; Plant Pathology; 69; 2; 10-2019; 393-402
0032-0862
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13109
info:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.13109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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