Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum

Autores
Carrillo Palomino, Roodney Alberto; Di Tocco, Aylen; Pierini, Gastón Dario; Porcal, Gabriela Valeria; Arevalo, Fernando Javier
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we developed a highly sensitive and reproducible electrochemiluminescent sensor based on a heterostructure of cadmium selenide quantum dots capped with 3-mercaptopropionic acid (MPA) + 3-morpholinoethanesulfonic acid (MES) (QDs CdSe) and carbon nitride nanosheets (g-C3N4) for the detection of H2O2 in lyophilized serum samples. To enhance the sensor sensitivity, g-C3N4 nanosheets were utilized as a platform to immobilize the QDs CdSe. An exhaustive characterization of the heterostructure was conducted, elucidating the interaction mechanism between QDs CdSe and g-C3N4. It was revealed that g-C3N4 acts as a hole (h+) donor, while QDs CdSe act as energy acceptors in a resonance energy transfer process, with the electrochemiluminescence emission originating from the QDs CdSe. The electrochemiluminescence intensity decreases in the presence of H2O2 due to the deactivation of the excited states of the QDs CdSe. This electrochemiluminescent sensor demonstrates exceptional performance for detecting H2O2 in aqueous systems, achieving a remarkably low limit of detection (LOD) of 1.81 nM, which is more sensitive than most reported sensors to detect H2O2. The applicability of the sensor was successfully tested where sub-µM levels of H2O2 were accurately quantified. These results highlight the potential of this electrochemiluminescent sensor as a reliable and pre-treatment-free tool for H2O2 detection in biochemical studies and human health applications.
Fil: Carrillo Palomino, Roodney Alberto. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Di Tocco, Aylen. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Pierini, Gastón Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Porcal, Gabriela Valeria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Materia
CADMIUN SELENIDE QUANTUM DOTS
GRAPHITIC CARBON NITRIDE
ELECTROCHELUMILINESCENCE
H2H2 DETECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281916
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human SerumCarrillo Palomino, Roodney AlbertoDi Tocco, AylenPierini, Gastón DarioPorcal, Gabriela ValeriaArevalo, Fernando JavierCADMIUN SELENIDE QUANTUM DOTSGRAPHITIC CARBON NITRIDEELECTROCHELUMILINESCENCEH2H2 DETECTIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, we developed a highly sensitive and reproducible electrochemiluminescent sensor based on a heterostructure of cadmium selenide quantum dots capped with 3-mercaptopropionic acid (MPA) + 3-morpholinoethanesulfonic acid (MES) (QDs CdSe) and carbon nitride nanosheets (g-C3N4) for the detection of H2O2 in lyophilized serum samples. To enhance the sensor sensitivity, g-C3N4 nanosheets were utilized as a platform to immobilize the QDs CdSe. An exhaustive characterization of the heterostructure was conducted, elucidating the interaction mechanism between QDs CdSe and g-C3N4. It was revealed that g-C3N4 acts as a hole (h+) donor, while QDs CdSe act as energy acceptors in a resonance energy transfer process, with the electrochemiluminescence emission originating from the QDs CdSe. The electrochemiluminescence intensity decreases in the presence of H2O2 due to the deactivation of the excited states of the QDs CdSe. This electrochemiluminescent sensor demonstrates exceptional performance for detecting H2O2 in aqueous systems, achieving a remarkably low limit of detection (LOD) of 1.81 nM, which is more sensitive than most reported sensors to detect H2O2. The applicability of the sensor was successfully tested where sub-µM levels of H2O2 were accurately quantified. These results highlight the potential of this electrochemiluminescent sensor as a reliable and pre-treatment-free tool for H2O2 detection in biochemical studies and human health applications.Fil: Carrillo Palomino, Roodney Alberto. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Di Tocco, Aylen. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Pierini, Gastón Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Porcal, Gabriela Valeria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaMultidisciplinary Digital Publishing Institute2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281916Carrillo Palomino, Roodney Alberto; Di Tocco, Aylen; Pierini, Gastón Dario; Porcal, Gabriela Valeria; Arevalo, Fernando Javier; Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum; Multidisciplinary Digital Publishing Institute; Chemosensors; 13; 5; 5-2025; 1-202227-9040CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9040/13/5/171info:eu-repo/semantics/altIdentifier/doi/10.3390/chemosensors13050171info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T14:57:05Zoai:ri.conicet.gov.ar:11336/281916instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 14:57:05.958CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
title Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
spellingShingle Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
Carrillo Palomino, Roodney Alberto
CADMIUN SELENIDE QUANTUM DOTS
GRAPHITIC CARBON NITRIDE
ELECTROCHELUMILINESCENCE
H2H2 DETECTION
title_short Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
title_full Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
title_fullStr Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
title_full_unstemmed Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
title_sort Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum
dc.creator.none.fl_str_mv Carrillo Palomino, Roodney Alberto
Di Tocco, Aylen
Pierini, Gastón Dario
Porcal, Gabriela Valeria
Arevalo, Fernando Javier
author Carrillo Palomino, Roodney Alberto
author_facet Carrillo Palomino, Roodney Alberto
Di Tocco, Aylen
Pierini, Gastón Dario
Porcal, Gabriela Valeria
Arevalo, Fernando Javier
author_role author
author2 Di Tocco, Aylen
Pierini, Gastón Dario
Porcal, Gabriela Valeria
Arevalo, Fernando Javier
author2_role author
author
author
author
dc.subject.none.fl_str_mv CADMIUN SELENIDE QUANTUM DOTS
GRAPHITIC CARBON NITRIDE
ELECTROCHELUMILINESCENCE
H2H2 DETECTION
topic CADMIUN SELENIDE QUANTUM DOTS
GRAPHITIC CARBON NITRIDE
ELECTROCHELUMILINESCENCE
H2H2 DETECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, we developed a highly sensitive and reproducible electrochemiluminescent sensor based on a heterostructure of cadmium selenide quantum dots capped with 3-mercaptopropionic acid (MPA) + 3-morpholinoethanesulfonic acid (MES) (QDs CdSe) and carbon nitride nanosheets (g-C3N4) for the detection of H2O2 in lyophilized serum samples. To enhance the sensor sensitivity, g-C3N4 nanosheets were utilized as a platform to immobilize the QDs CdSe. An exhaustive characterization of the heterostructure was conducted, elucidating the interaction mechanism between QDs CdSe and g-C3N4. It was revealed that g-C3N4 acts as a hole (h+) donor, while QDs CdSe act as energy acceptors in a resonance energy transfer process, with the electrochemiluminescence emission originating from the QDs CdSe. The electrochemiluminescence intensity decreases in the presence of H2O2 due to the deactivation of the excited states of the QDs CdSe. This electrochemiluminescent sensor demonstrates exceptional performance for detecting H2O2 in aqueous systems, achieving a remarkably low limit of detection (LOD) of 1.81 nM, which is more sensitive than most reported sensors to detect H2O2. The applicability of the sensor was successfully tested where sub-µM levels of H2O2 were accurately quantified. These results highlight the potential of this electrochemiluminescent sensor as a reliable and pre-treatment-free tool for H2O2 detection in biochemical studies and human health applications.
Fil: Carrillo Palomino, Roodney Alberto. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Di Tocco, Aylen. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Pierini, Gastón Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Porcal, Gabriela Valeria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Arevalo, Fernando Javier. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
description In this work, we developed a highly sensitive and reproducible electrochemiluminescent sensor based on a heterostructure of cadmium selenide quantum dots capped with 3-mercaptopropionic acid (MPA) + 3-morpholinoethanesulfonic acid (MES) (QDs CdSe) and carbon nitride nanosheets (g-C3N4) for the detection of H2O2 in lyophilized serum samples. To enhance the sensor sensitivity, g-C3N4 nanosheets were utilized as a platform to immobilize the QDs CdSe. An exhaustive characterization of the heterostructure was conducted, elucidating the interaction mechanism between QDs CdSe and g-C3N4. It was revealed that g-C3N4 acts as a hole (h+) donor, while QDs CdSe act as energy acceptors in a resonance energy transfer process, with the electrochemiluminescence emission originating from the QDs CdSe. The electrochemiluminescence intensity decreases in the presence of H2O2 due to the deactivation of the excited states of the QDs CdSe. This electrochemiluminescent sensor demonstrates exceptional performance for detecting H2O2 in aqueous systems, achieving a remarkably low limit of detection (LOD) of 1.81 nM, which is more sensitive than most reported sensors to detect H2O2. The applicability of the sensor was successfully tested where sub-µM levels of H2O2 were accurately quantified. These results highlight the potential of this electrochemiluminescent sensor as a reliable and pre-treatment-free tool for H2O2 detection in biochemical studies and human health applications.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281916
Carrillo Palomino, Roodney Alberto; Di Tocco, Aylen; Pierini, Gastón Dario; Porcal, Gabriela Valeria; Arevalo, Fernando Javier; Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum; Multidisciplinary Digital Publishing Institute; Chemosensors; 13; 5; 5-2025; 1-20
2227-9040
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281916
identifier_str_mv Carrillo Palomino, Roodney Alberto; Di Tocco, Aylen; Pierini, Gastón Dario; Porcal, Gabriela Valeria; Arevalo, Fernando Javier; Heterostructures of CdSe Quantum Dots and g-C3N4 Applied as Electrochemiluminescent Probes for the Detection of Hydrogen Peroxide in Human Serum; Multidisciplinary Digital Publishing Institute; Chemosensors; 13; 5; 5-2025; 1-20
2227-9040
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9040/13/5/171
info:eu-repo/semantics/altIdentifier/doi/10.3390/chemosensors13050171
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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