Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems

Autores
Hering, Vitor R.; Faulin, Tanize E. S.; Triboni, Eduardo R.; Rodríguez, Silvio David; Bernik, Delia Leticia; Schumacher, Robert I.; Mammana, Victor P.; Faljoni-Alario, Adelaide; Abdalla, Dulcinéia Saes Parra; Gibson, Gary; Politi, Mário J.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fluorescent proteins from the green fluorescent protein family strongly interact with CdSe/ZnS and ZnSe/ZnS nanocrystals at neutral pH. Green emitting CdSe/ZnS nanocrystals and red emitting fluorescent protein dTomato constitute a 72% efficiency FRET system with the largest alteration of the overall photoluminescence profile, following complex formation, observed so far. The substitution of ZnSe/ZnS for CdSe/ZnS nanocrystals as energy donors enabled the use of a green fluorescent protein, GFP5, as energy acceptor. Violet emitting ZnSe/ZnS nanocrystals and green GFP5 constitute a system with 43% FRET efficiency and an unusually strong sensitized emission. ZnSe/ZnS-GFP5 provides a cadmium-free, high-contrast FRET system that covers only the high-energy part of the visible spectrum, leaving room for simultaneous use of the yellow and red color channels. Anisotropic fluorescence measurements confirmed the depolarization of GFP5 sensitized emission.
Fil: Hering, Vitor R.. Universidade de Sao Paulo; Brasil
Fil: Faulin, Tanize E. S.. Universidade de Sao Paulo; Brasil
Fil: Triboni, Eduardo R.. Universidade de Sao Paulo; Brasil
Fil: Rodríguez, Silvio David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Bernik, Delia Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schumacher, Robert I.. Universidade de Sao Paulo; Brasil
Fil: Mammana, Victor P.. Centro de tecnologia da informacao Renato Archer; Brasil
Fil: Faljoni-Alario, Adelaide. Universidade Federal do ABC; Brasil
Fil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; Brasil
Fil: Gibson, Gary. Hewlett Packard Laboratories; . Hewlett Packard Laboratories; Estados Unidos
Fil: Politi, Mário J.. Universidade de Sao Paulo; Brasil
Materia
Nanocrystals
Fret
Green Fluorescent Protein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80442
Acceder
id CONICETDig_9aec88d24047e8b2f92906882772669c
oai_identifier_str oai:ri.conicet.gov.ar:11336/80442
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systemsHering, Vitor R.Faulin, Tanize E. S.Triboni, Eduardo R.Rodríguez, Silvio DavidBernik, Delia LeticiaSchumacher, Robert I.Mammana, Victor P.Faljoni-Alario, AdelaideAbdalla, Dulcinéia Saes ParraGibson, GaryPoliti, Mário J.NanocrystalsFretGreen Fluorescent Proteinhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Fluorescent proteins from the green fluorescent protein family strongly interact with CdSe/ZnS and ZnSe/ZnS nanocrystals at neutral pH. Green emitting CdSe/ZnS nanocrystals and red emitting fluorescent protein dTomato constitute a 72% efficiency FRET system with the largest alteration of the overall photoluminescence profile, following complex formation, observed so far. The substitution of ZnSe/ZnS for CdSe/ZnS nanocrystals as energy donors enabled the use of a green fluorescent protein, GFP5, as energy acceptor. Violet emitting ZnSe/ZnS nanocrystals and green GFP5 constitute a system with 43% FRET efficiency and an unusually strong sensitized emission. ZnSe/ZnS-GFP5 provides a cadmium-free, high-contrast FRET system that covers only the high-energy part of the visible spectrum, leaving room for simultaneous use of the yellow and red color channels. Anisotropic fluorescence measurements confirmed the depolarization of GFP5 sensitized emission.Fil: Hering, Vitor R.. Universidade de Sao Paulo; BrasilFil: Faulin, Tanize E. S.. Universidade de Sao Paulo; BrasilFil: Triboni, Eduardo R.. Universidade de Sao Paulo; BrasilFil: Rodríguez, Silvio David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Bernik, Delia Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schumacher, Robert I.. Universidade de Sao Paulo; BrasilFil: Mammana, Victor P.. Centro de tecnologia da informacao Renato Archer; BrasilFil: Faljoni-Alario, Adelaide. Universidade Federal do ABC; BrasilFil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; BrasilFil: Gibson, Gary. Hewlett Packard Laboratories; . Hewlett Packard Laboratories; Estados UnidosFil: Politi, Mário J.. Universidade de Sao Paulo; BrasilAmerican Chemical Society2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80442Hering, Vitor R.; Faulin, Tanize E. S.; Triboni, Eduardo R.; Rodríguez, Silvio David; Bernik, Delia Leticia; et al.; Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems; American Chemical Society; Bioconjugate Chemistry; 20; 6; 6-2009; 1237-12411043-1802CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/bc9001085info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/bc9001085info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:32Zoai:ri.conicet.gov.ar:11336/80442instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:32.697CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
title Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
spellingShingle Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
Hering, Vitor R.
Nanocrystals
Fret
Green Fluorescent Protein
title_short Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
title_full Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
title_fullStr Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
title_full_unstemmed Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
title_sort Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems
dc.creator.none.fl_str_mv Hering, Vitor R.
Faulin, Tanize E. S.
Triboni, Eduardo R.
Rodríguez, Silvio David
Bernik, Delia Leticia
Schumacher, Robert I.
Mammana, Victor P.
Faljoni-Alario, Adelaide
Abdalla, Dulcinéia Saes Parra
Gibson, Gary
Politi, Mário J.
author Hering, Vitor R.
author_facet Hering, Vitor R.
Faulin, Tanize E. S.
Triboni, Eduardo R.
Rodríguez, Silvio David
Bernik, Delia Leticia
Schumacher, Robert I.
Mammana, Victor P.
Faljoni-Alario, Adelaide
Abdalla, Dulcinéia Saes Parra
Gibson, Gary
Politi, Mário J.
author_role author
author2 Faulin, Tanize E. S.
Triboni, Eduardo R.
Rodríguez, Silvio David
Bernik, Delia Leticia
Schumacher, Robert I.
Mammana, Victor P.
Faljoni-Alario, Adelaide
Abdalla, Dulcinéia Saes Parra
Gibson, Gary
Politi, Mário J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanocrystals
Fret
Green Fluorescent Protein
topic Nanocrystals
Fret
Green Fluorescent Protein
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fluorescent proteins from the green fluorescent protein family strongly interact with CdSe/ZnS and ZnSe/ZnS nanocrystals at neutral pH. Green emitting CdSe/ZnS nanocrystals and red emitting fluorescent protein dTomato constitute a 72% efficiency FRET system with the largest alteration of the overall photoluminescence profile, following complex formation, observed so far. The substitution of ZnSe/ZnS for CdSe/ZnS nanocrystals as energy donors enabled the use of a green fluorescent protein, GFP5, as energy acceptor. Violet emitting ZnSe/ZnS nanocrystals and green GFP5 constitute a system with 43% FRET efficiency and an unusually strong sensitized emission. ZnSe/ZnS-GFP5 provides a cadmium-free, high-contrast FRET system that covers only the high-energy part of the visible spectrum, leaving room for simultaneous use of the yellow and red color channels. Anisotropic fluorescence measurements confirmed the depolarization of GFP5 sensitized emission.
Fil: Hering, Vitor R.. Universidade de Sao Paulo; Brasil
Fil: Faulin, Tanize E. S.. Universidade de Sao Paulo; Brasil
Fil: Triboni, Eduardo R.. Universidade de Sao Paulo; Brasil
Fil: Rodríguez, Silvio David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Bernik, Delia Leticia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schumacher, Robert I.. Universidade de Sao Paulo; Brasil
Fil: Mammana, Victor P.. Centro de tecnologia da informacao Renato Archer; Brasil
Fil: Faljoni-Alario, Adelaide. Universidade Federal do ABC; Brasil
Fil: Abdalla, Dulcinéia Saes Parra. Universidade de Sao Paulo; Brasil
Fil: Gibson, Gary. Hewlett Packard Laboratories; . Hewlett Packard Laboratories; Estados Unidos
Fil: Politi, Mário J.. Universidade de Sao Paulo; Brasil
description Fluorescent proteins from the green fluorescent protein family strongly interact with CdSe/ZnS and ZnSe/ZnS nanocrystals at neutral pH. Green emitting CdSe/ZnS nanocrystals and red emitting fluorescent protein dTomato constitute a 72% efficiency FRET system with the largest alteration of the overall photoluminescence profile, following complex formation, observed so far. The substitution of ZnSe/ZnS for CdSe/ZnS nanocrystals as energy donors enabled the use of a green fluorescent protein, GFP5, as energy acceptor. Violet emitting ZnSe/ZnS nanocrystals and green GFP5 constitute a system with 43% FRET efficiency and an unusually strong sensitized emission. ZnSe/ZnS-GFP5 provides a cadmium-free, high-contrast FRET system that covers only the high-energy part of the visible spectrum, leaving room for simultaneous use of the yellow and red color channels. Anisotropic fluorescence measurements confirmed the depolarization of GFP5 sensitized emission.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80442
Hering, Vitor R.; Faulin, Tanize E. S.; Triboni, Eduardo R.; Rodríguez, Silvio David; Bernik, Delia Leticia; et al.; Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems; American Chemical Society; Bioconjugate Chemistry; 20; 6; 6-2009; 1237-1241
1043-1802
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80442
identifier_str_mv Hering, Vitor R.; Faulin, Tanize E. S.; Triboni, Eduardo R.; Rodríguez, Silvio David; Bernik, Delia Leticia; et al.; Violet ZnSe/ZnS as an alternative to green CdSe/ZnS in nanocrystal- fluorescent protein FRET systems; American Chemical Society; Bioconjugate Chemistry; 20; 6; 6-2009; 1237-1241
1043-1802
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/bc9001085
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/bc9001085
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.260194

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