Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology

Autores
Guadagnoli, Tamara; Caltana, Laura Romina; Vacotto, Marina; Gironacci, Mariela Mercedes; Brusco, Herminia Alicia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The deleterious effects of ethanol (EtOH) on the brain have been widely described, but its effects on theneuronal cytoskeleton during differentiation have not yet been firmly established. In this context, ouraim was to investigate the direct effect of EtOH on cortical neurons during the period of differentiation.Primary cultures of cortical neurons obtained from 1-day-old rats were exposed to EtOH after 7 daysof culture, and viability and morphology were analyzed at structural and ultrastructural levels after24-h EtOH exposure. EtOH caused a significant reduction of 73±7% in the viability of cultured corticalneurons, by preferentially inducing apoptotic cellular death. This effect was accompanied by an increasein caspase 3 and 9 expression. Furthermore, EtOH induced a reduction in total dendrite length and in thenumber of dendrites per cell. Ultrastructural studies showed that EtOH increased the number of lipidicvacuoles, lysosomes and multilamellar vesicles and induced a dilated endoplasmatic reticulum lumenand a disorganized Golgi apparatus with a ring-shape appearance. Microtubules showed a disorganizeddistribution. Apposition between pre- and postsynaptic membranes without a defined synaptic cleft anda delay in presynaptic vesicle organization were also observed. Synaptophysin and PSD95 expression,proteins pre- and postsynaptically located, were reduced in EtOH-exposed cultures. Overall, our studyshows that EtOH induces neuronal apoptosis and changes in the cytoskeleton and membrane proteinsrelated with the establishment of mature synapses. These direct effects of EtOH on neurons may partiallyexplain its effects on brain development.
Fil: Guadagnoli, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Vacotto, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Materia
Ethanol
Neuronal Death
Neuronal Morphology
Synapse
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47040
Acceder
id CONICETDig_9ae8856acba2a339380ae79123ec05cf
oai_identifier_str oai:ri.conicet.gov.ar:11336/47040
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphologyGuadagnoli, TamaraCaltana, Laura RominaVacotto, MarinaGironacci, Mariela MercedesBrusco, Herminia AliciaEthanolNeuronal DeathNeuronal MorphologySynapsehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The deleterious effects of ethanol (EtOH) on the brain have been widely described, but its effects on theneuronal cytoskeleton during differentiation have not yet been firmly established. In this context, ouraim was to investigate the direct effect of EtOH on cortical neurons during the period of differentiation.Primary cultures of cortical neurons obtained from 1-day-old rats were exposed to EtOH after 7 daysof culture, and viability and morphology were analyzed at structural and ultrastructural levels after24-h EtOH exposure. EtOH caused a significant reduction of 73±7% in the viability of cultured corticalneurons, by preferentially inducing apoptotic cellular death. This effect was accompanied by an increasein caspase 3 and 9 expression. Furthermore, EtOH induced a reduction in total dendrite length and in thenumber of dendrites per cell. Ultrastructural studies showed that EtOH increased the number of lipidicvacuoles, lysosomes and multilamellar vesicles and induced a dilated endoplasmatic reticulum lumenand a disorganized Golgi apparatus with a ring-shape appearance. Microtubules showed a disorganizeddistribution. Apposition between pre- and postsynaptic membranes without a defined synaptic cleft anda delay in presynaptic vesicle organization were also observed. Synaptophysin and PSD95 expression,proteins pre- and postsynaptically located, were reduced in EtOH-exposed cultures. Overall, our studyshows that EtOH induces neuronal apoptosis and changes in the cytoskeleton and membrane proteinsrelated with the establishment of mature synapses. These direct effects of EtOH on neurons may partiallyexplain its effects on brain development.Fil: Guadagnoli, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Vacotto, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaPergamon-Elsevier Science Ltd2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47040Guadagnoli, Tamara; Caltana, Laura Romina; Vacotto, Marina; Gironacci, Mariela Mercedes; Brusco, Herminia Alicia; Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 127; 10-2016; 177-1860361-9230CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainresbull.2016.09.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0361923016302842info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:21:05Zoai:ri.conicet.gov.ar:11336/47040instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:21:05.635CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
title Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
spellingShingle Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
Guadagnoli, Tamara
Ethanol
Neuronal Death
Neuronal Morphology
Synapse
title_short Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
title_full Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
title_fullStr Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
title_full_unstemmed Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
title_sort Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology
dc.creator.none.fl_str_mv Guadagnoli, Tamara
Caltana, Laura Romina
Vacotto, Marina
Gironacci, Mariela Mercedes
Brusco, Herminia Alicia
author Guadagnoli, Tamara
author_facet Guadagnoli, Tamara
Caltana, Laura Romina
Vacotto, Marina
Gironacci, Mariela Mercedes
Brusco, Herminia Alicia
author_role author
author2 Caltana, Laura Romina
Vacotto, Marina
Gironacci, Mariela Mercedes
Brusco, Herminia Alicia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ethanol
Neuronal Death
Neuronal Morphology
Synapse
topic Ethanol
Neuronal Death
Neuronal Morphology
Synapse
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The deleterious effects of ethanol (EtOH) on the brain have been widely described, but its effects on theneuronal cytoskeleton during differentiation have not yet been firmly established. In this context, ouraim was to investigate the direct effect of EtOH on cortical neurons during the period of differentiation.Primary cultures of cortical neurons obtained from 1-day-old rats were exposed to EtOH after 7 daysof culture, and viability and morphology were analyzed at structural and ultrastructural levels after24-h EtOH exposure. EtOH caused a significant reduction of 73±7% in the viability of cultured corticalneurons, by preferentially inducing apoptotic cellular death. This effect was accompanied by an increasein caspase 3 and 9 expression. Furthermore, EtOH induced a reduction in total dendrite length and in thenumber of dendrites per cell. Ultrastructural studies showed that EtOH increased the number of lipidicvacuoles, lysosomes and multilamellar vesicles and induced a dilated endoplasmatic reticulum lumenand a disorganized Golgi apparatus with a ring-shape appearance. Microtubules showed a disorganizeddistribution. Apposition between pre- and postsynaptic membranes without a defined synaptic cleft anda delay in presynaptic vesicle organization were also observed. Synaptophysin and PSD95 expression,proteins pre- and postsynaptically located, were reduced in EtOH-exposed cultures. Overall, our studyshows that EtOH induces neuronal apoptosis and changes in the cytoskeleton and membrane proteinsrelated with the establishment of mature synapses. These direct effects of EtOH on neurons may partiallyexplain its effects on brain development.
Fil: Guadagnoli, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Caltana, Laura Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Vacotto, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
description The deleterious effects of ethanol (EtOH) on the brain have been widely described, but its effects on theneuronal cytoskeleton during differentiation have not yet been firmly established. In this context, ouraim was to investigate the direct effect of EtOH on cortical neurons during the period of differentiation.Primary cultures of cortical neurons obtained from 1-day-old rats were exposed to EtOH after 7 daysof culture, and viability and morphology were analyzed at structural and ultrastructural levels after24-h EtOH exposure. EtOH caused a significant reduction of 73±7% in the viability of cultured corticalneurons, by preferentially inducing apoptotic cellular death. This effect was accompanied by an increasein caspase 3 and 9 expression. Furthermore, EtOH induced a reduction in total dendrite length and in thenumber of dendrites per cell. Ultrastructural studies showed that EtOH increased the number of lipidicvacuoles, lysosomes and multilamellar vesicles and induced a dilated endoplasmatic reticulum lumenand a disorganized Golgi apparatus with a ring-shape appearance. Microtubules showed a disorganizeddistribution. Apposition between pre- and postsynaptic membranes without a defined synaptic cleft anda delay in presynaptic vesicle organization were also observed. Synaptophysin and PSD95 expression,proteins pre- and postsynaptically located, were reduced in EtOH-exposed cultures. Overall, our studyshows that EtOH induces neuronal apoptosis and changes in the cytoskeleton and membrane proteinsrelated with the establishment of mature synapses. These direct effects of EtOH on neurons may partiallyexplain its effects on brain development.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47040
Guadagnoli, Tamara; Caltana, Laura Romina; Vacotto, Marina; Gironacci, Mariela Mercedes; Brusco, Herminia Alicia; Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 127; 10-2016; 177-186
0361-9230
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47040
identifier_str_mv Guadagnoli, Tamara; Caltana, Laura Romina; Vacotto, Marina; Gironacci, Mariela Mercedes; Brusco, Herminia Alicia; Direct effects of ethanol on neuronal differentiation: An in vitro analysis of viability and morphology; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 127; 10-2016; 177-186
0361-9230
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainresbull.2016.09.013
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0361923016302842
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1852335093343846400
score 12.952241

Publicaciones similares