Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain

Autores
Arnal, Laura; Grunert, Tom; Cattelan, Natalia; De Gouw, Daan; Villalba, Maria I.; Serra, Diego Omar; Mooi, Frits R.; Ehling Schulz, Monika; Yantorno, Osvaldo Miguel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pertussis is a highly contagious disease mainly caused by Bordetella pertussis. Despite the massive use of vaccines, since the 1950s the disease has become re-emergent in 2000 with a shift in incidence from infants to adolescents and adults. Clearly, the efficacy of current cellular or acellular vaccines, formulated from bacteria grown in stirred bioreactors is limited, presenting a challenge for future vaccine development. For gaining insights into the role of B. pertussis biofilm development for host colonization and persistence within the host, we examined the biofilm forming capacity of eight argentinean clinical isolates recovered from 2001 to 2007. All clinical isolates showed an enhanced potential for biofilm formation compared to the reference strain Tohama I. We further selected the clinical isolate B. pertussis 2723, exhibiting the highest biofilm biomass production, for quantitative proteomic profiling by means of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry, which was accompanied by targeted transcriptional analysis. Results revealed an elevated expression of several virulence factors, including adhesins involved in biofilm development. In addition, we observed a higher expression of energy metabolism enzymes in the clinical isolate compared to the Tohama I strain. Furthermore, all clinical isolates carried a polymorphism in the bvgS gene. This mutation was associated to an increased sensitivity to modulation and a faster rate of adhesion to abiotic surfaces. Thus, the phenotypic biofilm characteristics shown by the clinical isolates might represent an important, hitherto underestimated, adaptive strategy for host colonization and long time persistence within the host.
Fil: Arnal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Grunert, Tom. University of Veterinary Medicine Vienna; Austria
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: De Gouw, Daan. Radboud Universiteit Nijmegen; Países Bajos
Fil: Villalba, Maria I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina. Universita Zu Berlin; Alemania
Fil: Mooi, Frits R.. National Institute for Public Health and the Environment; Países Bajos
Fil: Ehling Schulz, Monika. University of Veterinary Medicine Vienna; Austria
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Materia
WHOOPING COUGH
BORDETELLA PERTUSSIS
CLINICAL ISOLATES
PROTEOMIC
REAL TIME PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16115
Acceder
id CONICETDig_9accb010c81dad6c9b432008e3ba690a
oai_identifier_str oai:ri.conicet.gov.ar:11336/16115
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strainArnal, LauraGrunert, TomCattelan, NataliaDe Gouw, DaanVillalba, Maria I.Serra, Diego OmarMooi, Frits R.Ehling Schulz, MonikaYantorno, Osvaldo MiguelWHOOPING COUGHBORDETELLA PERTUSSISCLINICAL ISOLATESPROTEOMICREAL TIME PCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pertussis is a highly contagious disease mainly caused by Bordetella pertussis. Despite the massive use of vaccines, since the 1950s the disease has become re-emergent in 2000 with a shift in incidence from infants to adolescents and adults. Clearly, the efficacy of current cellular or acellular vaccines, formulated from bacteria grown in stirred bioreactors is limited, presenting a challenge for future vaccine development. For gaining insights into the role of B. pertussis biofilm development for host colonization and persistence within the host, we examined the biofilm forming capacity of eight argentinean clinical isolates recovered from 2001 to 2007. All clinical isolates showed an enhanced potential for biofilm formation compared to the reference strain Tohama I. We further selected the clinical isolate B. pertussis 2723, exhibiting the highest biofilm biomass production, for quantitative proteomic profiling by means of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry, which was accompanied by targeted transcriptional analysis. Results revealed an elevated expression of several virulence factors, including adhesins involved in biofilm development. In addition, we observed a higher expression of energy metabolism enzymes in the clinical isolate compared to the Tohama I strain. Furthermore, all clinical isolates carried a polymorphism in the bvgS gene. This mutation was associated to an increased sensitivity to modulation and a faster rate of adhesion to abiotic surfaces. Thus, the phenotypic biofilm characteristics shown by the clinical isolates might represent an important, hitherto underestimated, adaptive strategy for host colonization and long time persistence within the host.Fil: Arnal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Grunert, Tom. University of Veterinary Medicine Vienna; AustriaFil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: De Gouw, Daan. Radboud Universiteit Nijmegen; Países BajosFil: Villalba, Maria I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina. Universita Zu Berlin; AlemaniaFil: Mooi, Frits R.. National Institute for Public Health and the Environment; Países BajosFil: Ehling Schulz, Monika. University of Veterinary Medicine Vienna; AustriaFil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFrontiers2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16115Arnal, Laura; Grunert, Tom; Cattelan, Natalia; De Gouw, Daan; Villalba, Maria I.; et al.; Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain; Frontiers; Frontiers in Microbiology; 6; 12-2015; 1-12: 13521664-302Xenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2015.01352info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fmicb.2015.01352/fullinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672677/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:18Zoai:ri.conicet.gov.ar:11336/16115instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:18.356CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
spellingShingle Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
Arnal, Laura
WHOOPING COUGH
BORDETELLA PERTUSSIS
CLINICAL ISOLATES
PROTEOMIC
REAL TIME PCR
title_short Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_full Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_fullStr Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_full_unstemmed Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
title_sort Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain
dc.creator.none.fl_str_mv Arnal, Laura
Grunert, Tom
Cattelan, Natalia
De Gouw, Daan
Villalba, Maria I.
Serra, Diego Omar
Mooi, Frits R.
Ehling Schulz, Monika
Yantorno, Osvaldo Miguel
author Arnal, Laura
author_facet Arnal, Laura
Grunert, Tom
Cattelan, Natalia
De Gouw, Daan
Villalba, Maria I.
Serra, Diego Omar
Mooi, Frits R.
Ehling Schulz, Monika
Yantorno, Osvaldo Miguel
author_role author
author2 Grunert, Tom
Cattelan, Natalia
De Gouw, Daan
Villalba, Maria I.
Serra, Diego Omar
Mooi, Frits R.
Ehling Schulz, Monika
Yantorno, Osvaldo Miguel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv WHOOPING COUGH
BORDETELLA PERTUSSIS
CLINICAL ISOLATES
PROTEOMIC
REAL TIME PCR
topic WHOOPING COUGH
BORDETELLA PERTUSSIS
CLINICAL ISOLATES
PROTEOMIC
REAL TIME PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pertussis is a highly contagious disease mainly caused by Bordetella pertussis. Despite the massive use of vaccines, since the 1950s the disease has become re-emergent in 2000 with a shift in incidence from infants to adolescents and adults. Clearly, the efficacy of current cellular or acellular vaccines, formulated from bacteria grown in stirred bioreactors is limited, presenting a challenge for future vaccine development. For gaining insights into the role of B. pertussis biofilm development for host colonization and persistence within the host, we examined the biofilm forming capacity of eight argentinean clinical isolates recovered from 2001 to 2007. All clinical isolates showed an enhanced potential for biofilm formation compared to the reference strain Tohama I. We further selected the clinical isolate B. pertussis 2723, exhibiting the highest biofilm biomass production, for quantitative proteomic profiling by means of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry, which was accompanied by targeted transcriptional analysis. Results revealed an elevated expression of several virulence factors, including adhesins involved in biofilm development. In addition, we observed a higher expression of energy metabolism enzymes in the clinical isolate compared to the Tohama I strain. Furthermore, all clinical isolates carried a polymorphism in the bvgS gene. This mutation was associated to an increased sensitivity to modulation and a faster rate of adhesion to abiotic surfaces. Thus, the phenotypic biofilm characteristics shown by the clinical isolates might represent an important, hitherto underestimated, adaptive strategy for host colonization and long time persistence within the host.
Fil: Arnal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Grunert, Tom. University of Veterinary Medicine Vienna; Austria
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: De Gouw, Daan. Radboud Universiteit Nijmegen; Países Bajos
Fil: Villalba, Maria I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina. Universita Zu Berlin; Alemania
Fil: Mooi, Frits R.. National Institute for Public Health and the Environment; Países Bajos
Fil: Ehling Schulz, Monika. University of Veterinary Medicine Vienna; Austria
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
description Pertussis is a highly contagious disease mainly caused by Bordetella pertussis. Despite the massive use of vaccines, since the 1950s the disease has become re-emergent in 2000 with a shift in incidence from infants to adolescents and adults. Clearly, the efficacy of current cellular or acellular vaccines, formulated from bacteria grown in stirred bioreactors is limited, presenting a challenge for future vaccine development. For gaining insights into the role of B. pertussis biofilm development for host colonization and persistence within the host, we examined the biofilm forming capacity of eight argentinean clinical isolates recovered from 2001 to 2007. All clinical isolates showed an enhanced potential for biofilm formation compared to the reference strain Tohama I. We further selected the clinical isolate B. pertussis 2723, exhibiting the highest biofilm biomass production, for quantitative proteomic profiling by means of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with mass spectrometry, which was accompanied by targeted transcriptional analysis. Results revealed an elevated expression of several virulence factors, including adhesins involved in biofilm development. In addition, we observed a higher expression of energy metabolism enzymes in the clinical isolate compared to the Tohama I strain. Furthermore, all clinical isolates carried a polymorphism in the bvgS gene. This mutation was associated to an increased sensitivity to modulation and a faster rate of adhesion to abiotic surfaces. Thus, the phenotypic biofilm characteristics shown by the clinical isolates might represent an important, hitherto underestimated, adaptive strategy for host colonization and long time persistence within the host.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16115
Arnal, Laura; Grunert, Tom; Cattelan, Natalia; De Gouw, Daan; Villalba, Maria I.; et al.; Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain; Frontiers; Frontiers in Microbiology; 6; 12-2015; 1-12: 1352
1664-302X
url http://hdl.handle.net/11336/16115
identifier_str_mv Arnal, Laura; Grunert, Tom; Cattelan, Natalia; De Gouw, Daan; Villalba, Maria I.; et al.; Bordetella Pertussis isolates from argentinean whooping cough patients display enhanced biofilm formation capacity compared to Tohama I reference strain; Frontiers; Frontiers in Microbiology; 6; 12-2015; 1-12: 1352
1664-302X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2015.01352
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info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672677/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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