Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells

Autores
Laborde, Evangelina Andrea; Vanzulli, Silvia; Beigier Bompadre, Macarena; Isturiz, Martín Amadeo; Ruggiero, Raul Alejandro; Fourcade, Mariano G.; Catalan Pellet, Antonio C.; Sozzani, Silvano; Vulcano, Marisa
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The interaction between immune complexes (IC) and the receptors for the Fc portion of IgG (Fc Rs) triggers regulatory and effector functions in the immune system. In this study, we investigated the effects of IC on differentiation, maturation, and functions of human monocyte-derived dendritic cells (DC). When IC were added on day 0, DC generated on day 6 (IC-DC) showed lower levels of CD1a and increased expression of CD14, MHC class II, and the macrophage marker CD68, as compared with normally differentiated DC. The use of specific blocking Fc R mAbs indicated that the effect of IC was exerted mainly through their interaction with Fc RI and to a lesser extend with Fc RII. Immature IC-DC also expressed higher levels of CD83, CD86, and CD40 and the expression of these maturation markers was not further regulated by LPS. The apparent lack of maturation following TLR stimulation was associated with a decreased production of IL-12, normal secretion of IL-10 and CCL22, and increased production of CXCL8 and CCL2. IC-DC displayed low endocytic activity and a reduced ability to induce allogeneic T cell proliferation both at basal and LPS-stimulated conditions. Altogether, these data reveal that IC strongly affect DC differentiation and maturation. Skewing of DC function from Ag presentation to a proinflammatory phenotype by IC resembles the state of activation observed in DC obtained from patients with chronic inflammatory autoimmune disorders, such as systemic lupus erythematosus disease and arthritis. Therefore, the altered maturation of DC induced by IC may be involved in the pathogenesis of autoimmune diseases.
Fil: Laborde, Evangelina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Vanzulli, Silvia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; Argentina
Fil: Beigier Bompadre, Macarena. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ruggiero, Raul Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fourcade, Mariano G.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos Bernardino Rivadavia; Argentina
Fil: Catalan Pellet, Antonio C.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos Bernardino Rivadavia; Argentina
Fil: Sozzani, Silvano. Universita Degli Studi Di Brescia; Italia
Fil: Vulcano, Marisa. Humanitas Research Hospital; . Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
Antigen Antibody
Cell Differentiation
Dendritic Cells
Lupus Erythematosus
Monocytes
Receptors Igg
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57500
Acceder
id CONICETDig_9a7ad7efa4b4acc540f7beb86f0a8027
oai_identifier_str oai:ri.conicet.gov.ar:11336/57500
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cellsLaborde, Evangelina AndreaVanzulli, SilviaBeigier Bompadre, MacarenaIsturiz, Martín AmadeoRuggiero, Raul AlejandroFourcade, Mariano G.Catalan Pellet, Antonio C.Sozzani, SilvanoVulcano, MarisaAntigen AntibodyCell DifferentiationDendritic CellsLupus ErythematosusMonocytesReceptors Igghttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The interaction between immune complexes (IC) and the receptors for the Fc portion of IgG (Fc Rs) triggers regulatory and effector functions in the immune system. In this study, we investigated the effects of IC on differentiation, maturation, and functions of human monocyte-derived dendritic cells (DC). When IC were added on day 0, DC generated on day 6 (IC-DC) showed lower levels of CD1a and increased expression of CD14, MHC class II, and the macrophage marker CD68, as compared with normally differentiated DC. The use of specific blocking Fc R mAbs indicated that the effect of IC was exerted mainly through their interaction with Fc RI and to a lesser extend with Fc RII. Immature IC-DC also expressed higher levels of CD83, CD86, and CD40 and the expression of these maturation markers was not further regulated by LPS. The apparent lack of maturation following TLR stimulation was associated with a decreased production of IL-12, normal secretion of IL-10 and CCL22, and increased production of CXCL8 and CCL2. IC-DC displayed low endocytic activity and a reduced ability to induce allogeneic T cell proliferation both at basal and LPS-stimulated conditions. Altogether, these data reveal that IC strongly affect DC differentiation and maturation. Skewing of DC function from Ag presentation to a proinflammatory phenotype by IC resembles the state of activation observed in DC obtained from patients with chronic inflammatory autoimmune disorders, such as systemic lupus erythematosus disease and arthritis. Therefore, the altered maturation of DC induced by IC may be involved in the pathogenesis of autoimmune diseases.Fil: Laborde, Evangelina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Vanzulli, Silvia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; ArgentinaFil: Beigier Bompadre, Macarena. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Ruggiero, Raul Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Fourcade, Mariano G.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos Bernardino Rivadavia; ArgentinaFil: Catalan Pellet, Antonio C.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos Bernardino Rivadavia; ArgentinaFil: Sozzani, Silvano. Universita Degli Studi Di Brescia; ItaliaFil: Vulcano, Marisa. Humanitas Research Hospital; . Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaAmerican Association of Immunologists2007-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57500Laborde, Evangelina Andrea; Vanzulli, Silvia; Beigier Bompadre, Macarena; Isturiz, Martín Amadeo; Ruggiero, Raul Alejandro; et al.; Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells; American Association of Immunologists; Journal of Immunology; 179; 1; 1-7-2007; 673-6810022-1767CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.179.1.673info:eu-repo/semantics/altIdentifier/url/http://www.jimmunol.org/content/179/1/673.longinfo:eu-repo/semantics/altIdentifier/pmid/17579090info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:36Zoai:ri.conicet.gov.ar:11336/57500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:37.163CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
title Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
spellingShingle Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
Laborde, Evangelina Andrea
Antigen Antibody
Cell Differentiation
Dendritic Cells
Lupus Erythematosus
Monocytes
Receptors Igg
title_short Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
title_full Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
title_fullStr Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
title_full_unstemmed Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
title_sort Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells
dc.creator.none.fl_str_mv Laborde, Evangelina Andrea
Vanzulli, Silvia
Beigier Bompadre, Macarena
Isturiz, Martín Amadeo
Ruggiero, Raul Alejandro
Fourcade, Mariano G.
Catalan Pellet, Antonio C.
Sozzani, Silvano
Vulcano, Marisa
author Laborde, Evangelina Andrea
author_facet Laborde, Evangelina Andrea
Vanzulli, Silvia
Beigier Bompadre, Macarena
Isturiz, Martín Amadeo
Ruggiero, Raul Alejandro
Fourcade, Mariano G.
Catalan Pellet, Antonio C.
Sozzani, Silvano
Vulcano, Marisa
author_role author
author2 Vanzulli, Silvia
Beigier Bompadre, Macarena
Isturiz, Martín Amadeo
Ruggiero, Raul Alejandro
Fourcade, Mariano G.
Catalan Pellet, Antonio C.
Sozzani, Silvano
Vulcano, Marisa
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antigen Antibody
Cell Differentiation
Dendritic Cells
Lupus Erythematosus
Monocytes
Receptors Igg
topic Antigen Antibody
Cell Differentiation
Dendritic Cells
Lupus Erythematosus
Monocytes
Receptors Igg
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The interaction between immune complexes (IC) and the receptors for the Fc portion of IgG (Fc Rs) triggers regulatory and effector functions in the immune system. In this study, we investigated the effects of IC on differentiation, maturation, and functions of human monocyte-derived dendritic cells (DC). When IC were added on day 0, DC generated on day 6 (IC-DC) showed lower levels of CD1a and increased expression of CD14, MHC class II, and the macrophage marker CD68, as compared with normally differentiated DC. The use of specific blocking Fc R mAbs indicated that the effect of IC was exerted mainly through their interaction with Fc RI and to a lesser extend with Fc RII. Immature IC-DC also expressed higher levels of CD83, CD86, and CD40 and the expression of these maturation markers was not further regulated by LPS. The apparent lack of maturation following TLR stimulation was associated with a decreased production of IL-12, normal secretion of IL-10 and CCL22, and increased production of CXCL8 and CCL2. IC-DC displayed low endocytic activity and a reduced ability to induce allogeneic T cell proliferation both at basal and LPS-stimulated conditions. Altogether, these data reveal that IC strongly affect DC differentiation and maturation. Skewing of DC function from Ag presentation to a proinflammatory phenotype by IC resembles the state of activation observed in DC obtained from patients with chronic inflammatory autoimmune disorders, such as systemic lupus erythematosus disease and arthritis. Therefore, the altered maturation of DC induced by IC may be involved in the pathogenesis of autoimmune diseases.
Fil: Laborde, Evangelina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Vanzulli, Silvia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; Argentina
Fil: Beigier Bompadre, Macarena. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ruggiero, Raul Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fourcade, Mariano G.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos Bernardino Rivadavia; Argentina
Fil: Catalan Pellet, Antonio C.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos Bernardino Rivadavia; Argentina
Fil: Sozzani, Silvano. Universita Degli Studi Di Brescia; Italia
Fil: Vulcano, Marisa. Humanitas Research Hospital; . Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description The interaction between immune complexes (IC) and the receptors for the Fc portion of IgG (Fc Rs) triggers regulatory and effector functions in the immune system. In this study, we investigated the effects of IC on differentiation, maturation, and functions of human monocyte-derived dendritic cells (DC). When IC were added on day 0, DC generated on day 6 (IC-DC) showed lower levels of CD1a and increased expression of CD14, MHC class II, and the macrophage marker CD68, as compared with normally differentiated DC. The use of specific blocking Fc R mAbs indicated that the effect of IC was exerted mainly through their interaction with Fc RI and to a lesser extend with Fc RII. Immature IC-DC also expressed higher levels of CD83, CD86, and CD40 and the expression of these maturation markers was not further regulated by LPS. The apparent lack of maturation following TLR stimulation was associated with a decreased production of IL-12, normal secretion of IL-10 and CCL22, and increased production of CXCL8 and CCL2. IC-DC displayed low endocytic activity and a reduced ability to induce allogeneic T cell proliferation both at basal and LPS-stimulated conditions. Altogether, these data reveal that IC strongly affect DC differentiation and maturation. Skewing of DC function from Ag presentation to a proinflammatory phenotype by IC resembles the state of activation observed in DC obtained from patients with chronic inflammatory autoimmune disorders, such as systemic lupus erythematosus disease and arthritis. Therefore, the altered maturation of DC induced by IC may be involved in the pathogenesis of autoimmune diseases.
publishDate 2007
dc.date.none.fl_str_mv 2007-07-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57500
Laborde, Evangelina Andrea; Vanzulli, Silvia; Beigier Bompadre, Macarena; Isturiz, Martín Amadeo; Ruggiero, Raul Alejandro; et al.; Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells; American Association of Immunologists; Journal of Immunology; 179; 1; 1-7-2007; 673-681
0022-1767
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57500
identifier_str_mv Laborde, Evangelina Andrea; Vanzulli, Silvia; Beigier Bompadre, Macarena; Isturiz, Martín Amadeo; Ruggiero, Raul Alejandro; et al.; Immune complexes inhibit differentiation, maturation, and function of human monocyte-derived dendritic cells; American Association of Immunologists; Journal of Immunology; 179; 1; 1-7-2007; 673-681
0022-1767
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.179.1.673
info:eu-repo/semantics/altIdentifier/url/http://www.jimmunol.org/content/179/1/673.long
info:eu-repo/semantics/altIdentifier/pmid/17579090
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269919453904896
score 13.13397

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